This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 79.14 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 193,816 (6.4%) Reads that were too short: 119,594 (4.0%) Reads written (passing filters): 74,222 (2.5%) Total basepairs processed: 905,907,359 bp Total written (filtered): 18,231,050 bp (2.0%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 193816 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 631 734.8 0 631 7 415 734.8 0 415 8 39739 734.8 0 39739 9 5371 734.8 0 5371 10 52 734.8 0 52 11 41 734.8 0 41 12 39 734.8 0 39 13 64 734.8 0 64 14 60 734.8 0 60 15 32 734.8 0 32 16 46 734.8 0 46 17 41 734.8 0 41 18 260 734.8 0 260 19 51 734.8 0 51 20 43 734.8 0 43 21 65 734.8 0 65 22 30 734.8 0 30 23 25 734.8 0 25 24 46 734.8 0 46 25 35 734.8 0 35 26 34 734.8 0 34 27 25 734.8 0 25 28 32 734.8 0 32 29 33 734.8 0 33 30 44 734.8 0 44 31 71 734.8 0 71 32 47 734.8 0 47 33 45 734.8 0 45 34 31 734.8 0 31 35 34 734.8 0 34 36 23 734.8 0 23 37 45 734.8 0 45 38 77 734.8 0 77 39 32 734.8 0 32 40 45 734.8 0 45 41 143 734.8 0 143 42 30 734.8 0 30 43 100 734.8 0 100 44 121 734.8 0 121 45 77 734.8 0 77 46 1172 734.8 0 1172 47 208 734.8 0 208 48 88 734.8 0 88 49 48 734.8 0 48 50 50 734.8 0 50 51 50 734.8 0 50 52 102 734.8 0 102 53 231 734.8 0 231 54 242 734.8 0 242 55 51 734.8 0 51 56 58 734.8 0 58 57 443 734.8 0 443 58 46 734.8 0 46 59 42 734.8 0 42 60 37 734.8 0 37 61 26 734.8 0 26 62 43 734.8 0 43 63 19 734.8 0 19 64 46 734.8 0 46 65 44 734.8 0 44 66 65 734.8 0 65 67 31 734.8 0 31 68 30 734.8 0 30 69 32 734.8 0 32 70 21 734.8 0 21 71 31 734.8 0 31 72 34 734.8 0 34 73 58 734.8 0 58 74 40 734.8 0 40 75 51 734.8 0 51 76 46 734.8 0 46 77 64 734.8 0 64 78 63 734.8 0 63 79 33 734.8 0 33 80 26 734.8 0 26 81 32 734.8 0 32 82 70 734.8 0 70 83 63 734.8 0 63 84 84 734.8 0 84 85 92 734.8 0 92 86 91 734.8 0 91 87 56 734.8 0 56 88 63 734.8 0 63 89 91 734.8 0 91 90 58 734.8 0 58 91 61 734.8 0 61 92 71 734.8 0 71 93 71 734.8 0 71 94 144 734.8 0 144 95 91 734.8 0 91 96 90 734.8 0 90 97 115 734.8 0 115 98 122 734.8 0 122 99 148 734.8 0 148 100 67 734.8 0 67 101 46 734.8 0 46 102 65 734.8 0 65 103 61 734.8 0 61 104 46 734.8 0 46 105 53 734.8 0 53 106 75 734.8 0 75 107 120 734.8 0 120 108 96 734.8 0 96 109 47 734.8 0 47 110 86 734.8 0 86 111 77 734.8 0 77 112 90 734.8 0 90 113 70 734.8 0 70 114 103 734.8 0 103 115 138 734.8 0 138 116 140 734.8 0 140 117 120 734.8 0 120 118 125 734.8 0 125 119 171 734.8 0 171 120 216 734.8 0 216 121 113 734.8 0 113 122 67 734.8 0 67 123 72 734.8 0 72 124 121 734.8 0 121 125 133 734.8 0 133 126 165 734.8 0 165 127 163 734.8 0 163 128 127 734.8 0 127 129 106 734.8 0 106 130 114 734.8 0 114 131 82 734.8 0 82 132 82 734.8 0 82 133 111 734.8 0 111 134 215 734.8 0 215 135 342 734.8 0 342 136 285 734.8 0 285 137 234 734.8 0 234 138 163 734.8 0 163 139 124 734.8 0 124 140 129 734.8 0 129 141 137 734.8 0 137 142 105 734.8 0 105 143 112 734.8 0 112 144 144 734.8 0 144 145 711 734.8 0 711 146 389 734.8 0 389 147 350 734.8 0 350 148 125 734.8 0 125 149 114 734.8 0 114 150 106 734.8 0 106 151 118 734.8 0 118 152 132 734.8 0 132 153 193 734.8 0 193 154 209 734.8 0 209 155 173 734.8 0 173 156 166 734.8 0 166 157 197 734.8 0 197 158 202 734.8 0 202 159 192 734.8 0 192 160 144 734.8 0 144 161 137 734.8 0 137 162 157 734.8 0 157 163 119 734.8 0 119 164 188 734.8 0 188 165 364 734.8 0 364 166 342 734.8 0 342 167 208 734.8 0 208 168 151 734.8 0 151 169 145 734.8 0 145 170 135 734.8 0 135 171 160 734.8 0 160 172 165 734.8 0 165 173 849 734.8 0 849 174 1857 734.8 0 1857 175 867 734.8 0 867 176 288 734.8 0 288 177 101 734.8 0 101 178 147 734.8 0 147 179 167 734.8 0 167 180 219 734.8 0 219 181 362 734.8 0 362 182 470 734.8 0 470 183 381 734.8 0 381 184 233 734.8 0 233 185 148 734.8 0 148 186 320 734.8 0 320 187 243 734.8 0 243 188 212 734.8 0 212 189 182 734.8 0 182 190 178 734.8 0 178 191 247 734.8 0 247 192 356 734.8 0 356 193 417 734.8 0 417 194 227 734.8 0 227 195 109 734.8 0 109 196 93 734.8 0 93 197 152 734.8 0 152 198 235 734.8 0 235 199 181 734.8 0 181 200 120 734.8 0 120 201 152 734.8 0 152 202 223 734.8 0 223 203 265 734.8 0 265 204 285 734.8 0 285 205 225 734.8 0 225 206 300 734.8 0 300 207 244 734.8 0 244 208 198 734.8 0 198 209 128 734.8 0 128 210 139 734.8 0 139 211 178 734.8 0 178 212 200 734.8 0 200 213 222 734.8 0 222 214 296 734.8 0 296 215 237 734.8 0 237 216 228 734.8 0 228 217 227 734.8 0 227 218 292 734.8 0 292 219 334 734.8 0 334 220 343 734.8 0 343 221 317 734.8 0 317 222 323 734.8 0 323 223 282 734.8 0 282 224 345 734.8 0 345 225 4139 734.8 0 4139 226 8707 734.8 0 8707 227 1486 734.8 0 1486 228 249 734.8 0 249 229 335 734.8 0 335 230 842 734.8 0 842 231 1173 734.8 0 1173 232 1016 734.8 0 1016 233 589 734.8 0 589 234 379 734.8 0 379 235 332 734.8 0 332 236 210 734.8 0 210 237 252 734.8 0 252 238 221 734.8 0 221 239 287 734.8 0 287 240 201 734.8 0 201 241 214 734.8 0 214 242 261 734.8 0 261 243 224 734.8 0 224 244 207 734.8 0 207 245 201 734.8 0 201 246 451 734.8 0 451 247 425 734.8 0 425 248 223 734.8 0 223 249 93 734.8 0 93 250 2822 734.8 0 2822 251 4016 734.8 0 4016 252 2420 734.8 0 2420 253 820 734.8 0 820 254 643 734.8 0 643 255 297 734.8 0 297 256 277 734.8 0 277 257 243 734.8 0 243 258 252 734.8 0 252 259 158 734.8 0 158 260 203 734.8 0 203 261 231 734.8 0 231 262 226 734.8 0 226 263 236 734.8 0 236 264 226 734.8 0 226 265 693 734.8 0 693 266 477 734.8 0 477 267 359 734.8 0 359 268 213 734.8 0 213 269 183 734.8 0 183 270 236 734.8 0 236 271 664 734.8 0 664 272 1262 734.8 0 1262 273 362 734.8 0 362 274 419 734.8 0 419 275 900 734.8 0 900 276 556 734.8 0 556 277 314 734.8 0 314 278 381 734.8 0 381 279 578 734.8 0 578 280 1157 734.8 0 1157 281 999 734.8 0 999 282 680 734.8 0 680 283 467 734.8 0 467 284 703 734.8 0 703 285 1610 734.8 0 1610 286 1124 734.8 0 1124 287 610 734.8 0 610 288 544 734.8 0 544 289 532 734.8 0 532 290 1373 734.8 0 1373 291 1239 734.8 0 1239 292 985 734.8 0 985 293 541 734.8 0 541 294 545 734.8 0 545 295 915 734.8 0 915 296 1421 734.8 0 1421 297 2101 734.8 0 2101 298 3189 734.8 0 3189 299 5373 734.8 0 5373 300 9026 734.8 0 9026 301 35245 734.8 0 35245 Finished in 3.12 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 74,222 Reads with adapters: 40,518 (54.6%) Reads that were too short: 23 (0.0%) Reads written (passing filters): 40,495 (54.6%) Total basepairs processed: 18,231,050 bp Total written (filtered): 11,175,278 bp (61.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 40518 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 10 0.0 3 0 0 9 1 16 306 0.0 3 0 297 8 1 17 40004 0.0 3 39469 524 8 3 18 96 0.0 3 2 87 7 19 25 0.0 3 0 1 21 3 20 35 0.0 3 8 0 0 27 21 1 0.0 3 1 24 1 0.0 3 0 0 1 34 1 0.0 3 1 45 2 0.0 3 0 0 0 2 46 4 0.0 3 0 0 0 4 47 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 52 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 115 4 0.0 3 0 0 0 4 121 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 1 1 187 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 1 242 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.42 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 40,495 Reads with adapters: 1,373 (3.4%) Reads written (passing filters): 40,495 (100.0%) Total basepairs processed: 11,175,278 bp Total written (filtered): 11,171,110 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1373 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 67.8% C: 6.3% G: 24.2% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1324 632.7 0 1324 4 49 158.2 0 49