This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.36 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 173,807 (5.8%) Reads that were too short: 80,499 (2.7%) Reads written (passing filters): 93,308 (3.1%) Total basepairs processed: 905,907,359 bp Total written (filtered): 21,347,108 bp (2.4%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 173807 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 417 734.8 0 417 7 43232 734.8 0 43232 8 94 734.8 0 94 9 3993 734.8 0 3993 10 32 734.8 0 32 11 23 734.8 0 23 12 21 734.8 0 21 13 25 734.8 0 25 14 25 734.8 0 25 15 80 734.8 0 80 16 47 734.8 0 47 17 23 734.8 0 23 18 35 734.8 0 35 19 39 734.8 0 39 20 17 734.8 0 17 21 28 734.8 0 28 22 40 734.8 0 40 23 27 734.8 0 27 24 30 734.8 0 30 25 30 734.8 0 30 26 20 734.8 0 20 27 35 734.8 0 35 28 39 734.8 0 39 29 31 734.8 0 31 30 35 734.8 0 35 31 26 734.8 0 26 32 26 734.8 0 26 33 27 734.8 0 27 34 25 734.8 0 25 35 33 734.8 0 33 36 27 734.8 0 27 37 25 734.8 0 25 38 40 734.8 0 40 39 26 734.8 0 26 40 31 734.8 0 31 41 27 734.8 0 27 42 25 734.8 0 25 43 30 734.8 0 30 44 21 734.8 0 21 45 21 734.8 0 21 46 18 734.8 0 18 47 29 734.8 0 29 48 36 734.8 0 36 49 29 734.8 0 29 50 35 734.8 0 35 51 28 734.8 0 28 52 17 734.8 0 17 53 32 734.8 0 32 54 27 734.8 0 27 55 42 734.8 0 42 56 38 734.8 0 38 57 257 734.8 0 257 58 411 734.8 0 411 59 124 734.8 0 124 60 25 734.8 0 25 61 23 734.8 0 23 62 31 734.8 0 31 63 37 734.8 0 37 64 154 734.8 0 154 65 44 734.8 0 44 66 14 734.8 0 14 67 25 734.8 0 25 68 33 734.8 0 33 69 33 734.8 0 33 70 22 734.8 0 22 71 19 734.8 0 19 72 61 734.8 0 61 73 43 734.8 0 43 74 99 734.8 0 99 75 77 734.8 0 77 76 62 734.8 0 62 77 87 734.8 0 87 78 62 734.8 0 62 79 104 734.8 0 104 80 120 734.8 0 120 81 68 734.8 0 68 82 153 734.8 0 153 83 162 734.8 0 162 84 195 734.8 0 195 85 193 734.8 0 193 86 169 734.8 0 169 87 144 734.8 0 144 88 706 734.8 0 706 89 505 734.8 0 505 90 645 734.8 0 645 91 483 734.8 0 483 92 445 734.8 0 445 93 97 734.8 0 97 94 153 734.8 0 153 95 141 734.8 0 141 96 113 734.8 0 113 97 96 734.8 0 96 98 116 734.8 0 116 99 139 734.8 0 139 100 128 734.8 0 128 101 186 734.8 0 186 102 181 734.8 0 181 103 131 734.8 0 131 104 231 734.8 0 231 105 170 734.8 0 170 106 96 734.8 0 96 107 102 734.8 0 102 108 241 734.8 0 241 109 216 734.8 0 216 110 126 734.8 0 126 111 85 734.8 0 85 112 139 734.8 0 139 113 128 734.8 0 128 114 142 734.8 0 142 115 236 734.8 0 236 116 244 734.8 0 244 117 154 734.8 0 154 118 77 734.8 0 77 119 99 734.8 0 99 120 113 734.8 0 113 121 307 734.8 0 307 122 1101 734.8 0 1101 123 848 734.8 0 848 124 320 734.8 0 320 125 164 734.8 0 164 126 166 734.8 0 166 127 191 734.8 0 191 128 187 734.8 0 187 129 190 734.8 0 190 130 273 734.8 0 273 131 298 734.8 0 298 132 175 734.8 0 175 133 136 734.8 0 136 134 245 734.8 0 245 135 251 734.8 0 251 136 296 734.8 0 296 137 214 734.8 0 214 138 190 734.8 0 190 139 182 734.8 0 182 140 298 734.8 0 298 141 1098 734.8 0 1098 142 975 734.8 0 975 143 480 734.8 0 480 144 164 734.8 0 164 145 185 734.8 0 185 146 275 734.8 0 275 147 446 734.8 0 446 148 754 734.8 0 754 149 331 734.8 0 331 150 329 734.8 0 329 151 736 734.8 0 736 152 2668 734.8 0 2668 153 501 734.8 0 501 154 380 734.8 0 380 155 352 734.8 0 352 156 292 734.8 0 292 157 652 734.8 0 652 158 754 734.8 0 754 159 396 734.8 0 396 160 294 734.8 0 294 161 369 734.8 0 369 162 557 734.8 0 557 163 369 734.8 0 369 164 196 734.8 0 196 165 221 734.8 0 221 166 331 734.8 0 331 167 404 734.8 0 404 168 327 734.8 0 327 169 310 734.8 0 310 170 437 734.8 0 437 171 4940 734.8 0 4940 172 255 734.8 0 255 173 114 734.8 0 114 174 172 734.8 0 172 175 323 734.8 0 323 176 332 734.8 0 332 177 237 734.8 0 237 178 185 734.8 0 185 179 133 734.8 0 133 180 137 734.8 0 137 181 194 734.8 0 194 182 528 734.8 0 528 183 634 734.8 0 634 184 337 734.8 0 337 185 226 734.8 0 226 186 331 734.8 0 331 187 384 734.8 0 384 188 341 734.8 0 341 189 238 734.8 0 238 190 179 734.8 0 179 191 177 734.8 0 177 192 144 734.8 0 144 193 193 734.8 0 193 194 170 734.8 0 170 195 238 734.8 0 238 196 458 734.8 0 458 197 293 734.8 0 293 198 286 734.8 0 286 199 147 734.8 0 147 200 116 734.8 0 116 201 177 734.8 0 177 202 250 734.8 0 250 203 354 734.8 0 354 204 274 734.8 0 274 205 290 734.8 0 290 206 292 734.8 0 292 207 334 734.8 0 334 208 547 734.8 0 547 209 1194 734.8 0 1194 210 1268 734.8 0 1268 211 943 734.8 0 943 212 908 734.8 0 908 213 12293 734.8 0 12293 214 20757 734.8 0 20757 215 3727 734.8 0 3727 216 409 734.8 0 409 217 313 734.8 0 313 218 307 734.8 0 307 219 233 734.8 0 233 220 223 734.8 0 223 221 2099 734.8 0 2099 222 3153 734.8 0 3153 223 1362 734.8 0 1362 224 372 734.8 0 372 225 224 734.8 0 224 226 321 734.8 0 321 227 291 734.8 0 291 228 242 734.8 0 242 229 192 734.8 0 192 230 132 734.8 0 132 231 246 734.8 0 246 232 286 734.8 0 286 233 302 734.8 0 302 234 336 734.8 0 336 235 432 734.8 0 432 236 210 734.8 0 210 237 218 734.8 0 218 238 171 734.8 0 171 239 172 734.8 0 172 240 153 734.8 0 153 241 135 734.8 0 135 242 250 734.8 0 250 243 563 734.8 0 563 244 978 734.8 0 978 245 540 734.8 0 540 246 283 734.8 0 283 247 212 734.8 0 212 248 111 734.8 0 111 249 62 734.8 0 62 250 66 734.8 0 66 251 158 734.8 0 158 252 282 734.8 0 282 253 420 734.8 0 420 254 390 734.8 0 390 255 401 734.8 0 401 256 437 734.8 0 437 257 1786 734.8 0 1786 258 2363 734.8 0 2363 259 559 734.8 0 559 260 162 734.8 0 162 261 221 734.8 0 221 262 229 734.8 0 229 263 510 734.8 0 510 264 705 734.8 0 705 265 482 734.8 0 482 266 311 734.8 0 311 267 268 734.8 0 268 268 239 734.8 0 239 269 143 734.8 0 143 270 273 734.8 0 273 271 695 734.8 0 695 272 647 734.8 0 647 273 507 734.8 0 507 274 313 734.8 0 313 275 245 734.8 0 245 276 229 734.8 0 229 277 208 734.8 0 208 278 575 734.8 0 575 279 1169 734.8 0 1169 280 267 734.8 0 267 281 235 734.8 0 235 282 412 734.8 0 412 283 285 734.8 0 285 284 745 734.8 0 745 285 1168 734.8 0 1168 286 445 734.8 0 445 287 168 734.8 0 168 288 88 734.8 0 88 289 110 734.8 0 110 290 233 734.8 0 233 291 435 734.8 0 435 292 331 734.8 0 331 293 172 734.8 0 172 294 204 734.8 0 204 295 251 734.8 0 251 296 166 734.8 0 166 297 167 734.8 0 167 298 206 734.8 0 206 299 196 734.8 0 196 300 111 734.8 0 111 301 147 734.8 0 147 Finished in 4.04 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 93,308 Reads with adapters: 43,594 (46.7%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 43,582 (46.7%) Total basepairs processed: 21,347,108 bp Total written (filtered): 11,927,401 bp (55.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 43594 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 21 0.0 3 0 0 17 2 2 19 398 0.0 3 0 379 8 7 4 20 42857 0.0 4 41965 772 79 29 12 21 116 0.0 4 25 85 2 3 1 22 17 0.0 4 7 0 8 0 2 23 38 0.0 4 2 0 1 30 5 25 1 0.0 4 0 1 27 13 0.0 4 0 0 0 0 13 28 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 32 1 0.0 4 1 40 1 0.0 4 0 0 0 0 1 44 2 0.0 4 0 0 0 1 1 51 3 0.0 4 0 0 0 3 57 2 0.0 4 0 0 0 0 2 69 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 111 30 0.0 4 0 0 0 26 4 125 1 0.0 4 0 0 0 0 1 127 77 0.0 4 0 0 0 0 77 128 2 0.0 4 0 0 0 0 2 142 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.50 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 43,582 Reads with adapters: 1,925 (4.4%) Reads written (passing filters): 43,582 (100.0%) Total basepairs processed: 11,927,401 bp Total written (filtered): 11,901,586 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1925 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.9% C: 3.8% G: 91.4% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 13 681.0 0 13 4 2 170.2 0 2 9 1 0.2 0 0 1 10 1 0.0 1 0 1 11 1799 0.0 1 21 1778 12 80 0.0 1 0 80 13 3 0.0 1 0 3 15 2 0.0 1 0 2 25 1 0.0 1 1 152 2 0.0 1 2 210 15 0.0 1 10 5 211 1 0.0 1 0 1 225 2 0.0 1 2 260 1 0.0 1 1 261 1 0.0 1 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.