This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 81.17 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 85,041 (2.8%) Reads that were too short: 20,726 (0.7%) Reads written (passing filters): 64,315 (2.1%) Total basepairs processed: 905,907,359 bp Total written (filtered): 17,071,682 bp (1.9%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 85041 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 608 734.8 0 608 7 42659 734.8 0 42659 8 3655 734.8 0 3655 9 55 734.8 0 55 10 46 734.8 0 46 11 43 734.8 0 43 12 36 734.8 0 36 13 39 734.8 0 39 14 43 734.8 0 43 15 39 734.8 0 39 16 37 734.8 0 37 17 39 734.8 0 39 18 41 734.8 0 41 19 44 734.8 0 44 20 44 734.8 0 44 21 59 734.8 0 59 22 49 734.8 0 49 23 98 734.8 0 98 24 32 734.8 0 32 25 21 734.8 0 21 26 34 734.8 0 34 27 33 734.8 0 33 28 45 734.8 0 45 29 51 734.8 0 51 30 55 734.8 0 55 31 88 734.8 0 88 32 36 734.8 0 36 33 44 734.8 0 44 34 38 734.8 0 38 35 46 734.8 0 46 36 35 734.8 0 35 37 39 734.8 0 39 38 52 734.8 0 52 39 206 734.8 0 206 40 95 734.8 0 95 41 39 734.8 0 39 42 37 734.8 0 37 43 59 734.8 0 59 44 31 734.8 0 31 45 27 734.8 0 27 46 41 734.8 0 41 47 29 734.8 0 29 48 40 734.8 0 40 49 41 734.8 0 41 50 42 734.8 0 42 51 38 734.8 0 38 52 38 734.8 0 38 53 48 734.8 0 48 54 48 734.8 0 48 55 26 734.8 0 26 56 22 734.8 0 22 57 50 734.8 0 50 58 43 734.8 0 43 59 27 734.8 0 27 60 38 734.8 0 38 61 49 734.8 0 49 62 37 734.8 0 37 63 36 734.8 0 36 64 90 734.8 0 90 65 3124 734.8 0 3124 66 146 734.8 0 146 67 46 734.8 0 46 68 37 734.8 0 37 69 36 734.8 0 36 70 64 734.8 0 64 71 69 734.8 0 69 72 49 734.8 0 49 73 40 734.8 0 40 74 42 734.8 0 42 75 39 734.8 0 39 76 30 734.8 0 30 77 38 734.8 0 38 78 34 734.8 0 34 79 46 734.8 0 46 80 45 734.8 0 45 81 54 734.8 0 54 82 58 734.8 0 58 83 50 734.8 0 50 84 41 734.8 0 41 85 88 734.8 0 88 86 95 734.8 0 95 87 42 734.8 0 42 88 30 734.8 0 30 89 38 734.8 0 38 90 36 734.8 0 36 91 43 734.8 0 43 92 51 734.8 0 51 93 44 734.8 0 44 94 38 734.8 0 38 95 61 734.8 0 61 96 92 734.8 0 92 97 69 734.8 0 69 98 207 734.8 0 207 99 187 734.8 0 187 100 78 734.8 0 78 101 79 734.8 0 79 102 119 734.8 0 119 103 125 734.8 0 125 104 83 734.8 0 83 105 57 734.8 0 57 106 35 734.8 0 35 107 56 734.8 0 56 108 34 734.8 0 34 109 40 734.8 0 40 110 66 734.8 0 66 111 84 734.8 0 84 112 71 734.8 0 71 113 73 734.8 0 73 114 50 734.8 0 50 115 53 734.8 0 53 116 74 734.8 0 74 117 92 734.8 0 92 118 64 734.8 0 64 119 52 734.8 0 52 120 64 734.8 0 64 121 48 734.8 0 48 122 47 734.8 0 47 123 96 734.8 0 96 124 133 734.8 0 133 125 116 734.8 0 116 126 63 734.8 0 63 127 72 734.8 0 72 128 46 734.8 0 46 129 64 734.8 0 64 130 56 734.8 0 56 131 83 734.8 0 83 132 108 734.8 0 108 133 163 734.8 0 163 134 72 734.8 0 72 135 105 734.8 0 105 136 70 734.8 0 70 137 89 734.8 0 89 138 132 734.8 0 132 139 128 734.8 0 128 140 63 734.8 0 63 141 59 734.8 0 59 142 73 734.8 0 73 143 148 734.8 0 148 144 143 734.8 0 143 145 113 734.8 0 113 146 69 734.8 0 69 147 126 734.8 0 126 148 95 734.8 0 95 149 90 734.8 0 90 150 75 734.8 0 75 151 72 734.8 0 72 152 58 734.8 0 58 153 75 734.8 0 75 154 60 734.8 0 60 155 60 734.8 0 60 156 63 734.8 0 63 157 73 734.8 0 73 158 131 734.8 0 131 159 157 734.8 0 157 160 114 734.8 0 114 161 91 734.8 0 91 162 109 734.8 0 109 163 68 734.8 0 68 164 72 734.8 0 72 165 55 734.8 0 55 166 74 734.8 0 74 167 137 734.8 0 137 168 114 734.8 0 114 169 128 734.8 0 128 170 156 734.8 0 156 171 184 734.8 0 184 172 136 734.8 0 136 173 77 734.8 0 77 174 74 734.8 0 74 175 59 734.8 0 59 176 64 734.8 0 64 177 147 734.8 0 147 178 153 734.8 0 153 179 141 734.8 0 141 180 122 734.8 0 122 181 78 734.8 0 78 182 85 734.8 0 85 183 118 734.8 0 118 184 232 734.8 0 232 185 127 734.8 0 127 186 82 734.8 0 82 187 178 734.8 0 178 188 150 734.8 0 150 189 137 734.8 0 137 190 104 734.8 0 104 191 54 734.8 0 54 192 96 734.8 0 96 193 83 734.8 0 83 194 132 734.8 0 132 195 98 734.8 0 98 196 94 734.8 0 94 197 97 734.8 0 97 198 135 734.8 0 135 199 143 734.8 0 143 200 48 734.8 0 48 201 57 734.8 0 57 202 51 734.8 0 51 203 50 734.8 0 50 204 95 734.8 0 95 205 73 734.8 0 73 206 92 734.8 0 92 207 96 734.8 0 96 208 56 734.8 0 56 209 61 734.8 0 61 210 125 734.8 0 125 211 169 734.8 0 169 212 139 734.8 0 139 213 112 734.8 0 112 214 540 734.8 0 540 215 556 734.8 0 556 216 379 734.8 0 379 217 109 734.8 0 109 218 84 734.8 0 84 219 96 734.8 0 96 220 74 734.8 0 74 221 54 734.8 0 54 222 69 734.8 0 69 223 305 734.8 0 305 224 620 734.8 0 620 225 242 734.8 0 242 226 77 734.8 0 77 227 94 734.8 0 94 228 111 734.8 0 111 229 160 734.8 0 160 230 71 734.8 0 71 231 62 734.8 0 62 232 50 734.8 0 50 233 55 734.8 0 55 234 58 734.8 0 58 235 56 734.8 0 56 236 83 734.8 0 83 237 83 734.8 0 83 238 141 734.8 0 141 239 180 734.8 0 180 240 172 734.8 0 172 241 131 734.8 0 131 242 63 734.8 0 63 243 50 734.8 0 50 244 45 734.8 0 45 245 88 734.8 0 88 246 152 734.8 0 152 247 212 734.8 0 212 248 106 734.8 0 106 249 59 734.8 0 59 250 25 734.8 0 25 251 44 734.8 0 44 252 46 734.8 0 46 253 58 734.8 0 58 254 67 734.8 0 67 255 140 734.8 0 140 256 108 734.8 0 108 257 141 734.8 0 141 258 174 734.8 0 174 259 118 734.8 0 118 260 161 734.8 0 161 261 54 734.8 0 54 262 59 734.8 0 59 263 144 734.8 0 144 264 216 734.8 0 216 265 159 734.8 0 159 266 106 734.8 0 106 267 227 734.8 0 227 268 224 734.8 0 224 269 246 734.8 0 246 270 126 734.8 0 126 271 197 734.8 0 197 272 3370 734.8 0 3370 273 2942 734.8 0 2942 274 1222 734.8 0 1222 275 85 734.8 0 85 276 104 734.8 0 104 277 116 734.8 0 116 278 113 734.8 0 113 279 233 734.8 0 233 280 77 734.8 0 77 281 35 734.8 0 35 282 55 734.8 0 55 283 120 734.8 0 120 284 173 734.8 0 173 285 199 734.8 0 199 286 181 734.8 0 181 287 209 734.8 0 209 288 118 734.8 0 118 289 41 734.8 0 41 290 36 734.8 0 36 291 42 734.8 0 42 292 58 734.8 0 58 293 131 734.8 0 131 294 109 734.8 0 109 295 84 734.8 0 84 296 52 734.8 0 52 297 55 734.8 0 55 298 58 734.8 0 58 299 137 734.8 0 137 300 253 734.8 0 253 301 872 734.8 0 872 Finished in 2.87 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 64,315 Reads with adapters: 43,001 (66.9%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 42,991 (66.8%) Total basepairs processed: 17,071,682 bp Total written (filtered): 11,907,288 bp (69.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 43001 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 9 0.0 3 0 0 9 16 266 0.0 3 0 248 18 17 42505 0.0 3 41944 541 16 4 18 119 0.0 3 8 107 4 19 23 0.0 3 0 0 20 3 20 53 0.0 3 0 0 0 53 21 1 0.0 3 0 0 1 25 1 0.0 3 1 28 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 248 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.52 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 42,991 Reads with adapters: 2,334 (5.4%) Reads written (passing filters): 42,991 (100.0%) Total basepairs processed: 11,907,288 bp Total written (filtered): 11,900,281 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2334 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.8% C: 68.9% G: 10.0% T: 0.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2329 671.7 0 2329 4 5 167.9 0 5