This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.83 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 304,949 (10.1%) Reads that were too short: 13,366 (0.4%) Reads written (passing filters): 291,583 (9.7%) Total basepairs processed: 905,907,359 bp Total written (filtered): 71,037,706 bp (7.8%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 304949 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1004 734.8 0 1004 7 1250 734.8 0 1250 8 64307 734.8 0 64307 9 32305 734.8 0 32305 10 91 734.8 0 91 11 50 734.8 0 50 12 58 734.8 0 58 13 45 734.8 0 45 14 47 734.8 0 47 15 66 734.8 0 66 16 48 734.8 0 48 17 46 734.8 0 46 18 65 734.8 0 65 19 47 734.8 0 47 20 51 734.8 0 51 21 61 734.8 0 61 22 81 734.8 0 81 23 85 734.8 0 85 24 69 734.8 0 69 25 53 734.8 0 53 26 59 734.8 0 59 27 51 734.8 0 51 28 44 734.8 0 44 29 62 734.8 0 62 30 68 734.8 0 68 31 59 734.8 0 59 32 70 734.8 0 70 33 63 734.8 0 63 34 84 734.8 0 84 35 79 734.8 0 79 36 86 734.8 0 86 37 84 734.8 0 84 38 123 734.8 0 123 39 83 734.8 0 83 40 89 734.8 0 89 41 56 734.8 0 56 42 94 734.8 0 94 43 68 734.8 0 68 44 112 734.8 0 112 45 153 734.8 0 153 46 164 734.8 0 164 47 249 734.8 0 249 48 466 734.8 0 466 49 1349 734.8 0 1349 50 520 734.8 0 520 51 750 734.8 0 750 52 538 734.8 0 538 53 342 734.8 0 342 54 408 734.8 0 408 55 600 734.8 0 600 56 494 734.8 0 494 57 830 734.8 0 830 58 777 734.8 0 777 59 1150 734.8 0 1150 60 1283 734.8 0 1283 61 2348 734.8 0 2348 62 57633 734.8 0 57633 63 61190 734.8 0 61190 64 12855 734.8 0 12855 65 183 734.8 0 183 66 69 734.8 0 69 67 43 734.8 0 43 68 51 734.8 0 51 69 54 734.8 0 54 70 74 734.8 0 74 71 84 734.8 0 84 72 123 734.8 0 123 73 95 734.8 0 95 74 106 734.8 0 106 75 103 734.8 0 103 76 1061 734.8 0 1061 77 3125 734.8 0 3125 78 256 734.8 0 256 79 125 734.8 0 125 80 53 734.8 0 53 81 51 734.8 0 51 82 61 734.8 0 61 83 63 734.8 0 63 84 83 734.8 0 83 85 66 734.8 0 66 86 70 734.8 0 70 87 82 734.8 0 82 88 56 734.8 0 56 89 51 734.8 0 51 90 77 734.8 0 77 91 64 734.8 0 64 92 72 734.8 0 72 93 118 734.8 0 118 94 112 734.8 0 112 95 51 734.8 0 51 96 27 734.8 0 27 97 41 734.8 0 41 98 41 734.8 0 41 99 61 734.8 0 61 100 41 734.8 0 41 101 72 734.8 0 72 102 49 734.8 0 49 103 57 734.8 0 57 104 60 734.8 0 60 105 78 734.8 0 78 106 71 734.8 0 71 107 228 734.8 0 228 108 408 734.8 0 408 109 60 734.8 0 60 110 69 734.8 0 69 111 71 734.8 0 71 112 61 734.8 0 61 113 46 734.8 0 46 114 46 734.8 0 46 115 52 734.8 0 52 116 39 734.8 0 39 117 68 734.8 0 68 118 57 734.8 0 57 119 66 734.8 0 66 120 61 734.8 0 61 121 68 734.8 0 68 122 63 734.8 0 63 123 64 734.8 0 64 124 65 734.8 0 65 125 60 734.8 0 60 126 100 734.8 0 100 127 98 734.8 0 98 128 74 734.8 0 74 129 83 734.8 0 83 130 59 734.8 0 59 131 53 734.8 0 53 132 81 734.8 0 81 133 101 734.8 0 101 134 88 734.8 0 88 135 88 734.8 0 88 136 63 734.8 0 63 137 71 734.8 0 71 138 75 734.8 0 75 139 84 734.8 0 84 140 88 734.8 0 88 141 128 734.8 0 128 142 264 734.8 0 264 143 210 734.8 0 210 144 2088 734.8 0 2088 145 4043 734.8 0 4043 146 504 734.8 0 504 147 142 734.8 0 142 148 74 734.8 0 74 149 123 734.8 0 123 150 191 734.8 0 191 151 137 734.8 0 137 152 94 734.8 0 94 153 99 734.8 0 99 154 114 734.8 0 114 155 141 734.8 0 141 156 1057 734.8 0 1057 157 1086 734.8 0 1086 158 184 734.8 0 184 159 150 734.8 0 150 160 146 734.8 0 146 161 159 734.8 0 159 162 248 734.8 0 248 163 294 734.8 0 294 164 3886 734.8 0 3886 165 4843 734.8 0 4843 166 668 734.8 0 668 167 138 734.8 0 138 168 159 734.8 0 159 169 1165 734.8 0 1165 170 2779 734.8 0 2779 171 1787 734.8 0 1787 172 1190 734.8 0 1190 173 645 734.8 0 645 174 217 734.8 0 217 175 82 734.8 0 82 176 61 734.8 0 61 177 70 734.8 0 70 178 88 734.8 0 88 179 155 734.8 0 155 180 135 734.8 0 135 181 164 734.8 0 164 182 126 734.8 0 126 183 116 734.8 0 116 184 169 734.8 0 169 185 210 734.8 0 210 186 212 734.8 0 212 187 128 734.8 0 128 188 186 734.8 0 186 189 1556 734.8 0 1556 190 1735 734.8 0 1735 191 863 734.8 0 863 192 187 734.8 0 187 193 74 734.8 0 74 194 42 734.8 0 42 195 65 734.8 0 65 196 72 734.8 0 72 197 94 734.8 0 94 198 107 734.8 0 107 199 77 734.8 0 77 200 79 734.8 0 79 201 77 734.8 0 77 202 61 734.8 0 61 203 74 734.8 0 74 204 76 734.8 0 76 205 65 734.8 0 65 206 122 734.8 0 122 207 106 734.8 0 106 208 111 734.8 0 111 209 83 734.8 0 83 210 71 734.8 0 71 211 63 734.8 0 63 212 83 734.8 0 83 213 99 734.8 0 99 214 101 734.8 0 101 215 97 734.8 0 97 216 232 734.8 0 232 217 256 734.8 0 256 218 187 734.8 0 187 219 64 734.8 0 64 220 54 734.8 0 54 221 57 734.8 0 57 222 63 734.8 0 63 223 59 734.8 0 59 224 65 734.8 0 65 225 100 734.8 0 100 226 192 734.8 0 192 227 174 734.8 0 174 228 146 734.8 0 146 229 252 734.8 0 252 230 181 734.8 0 181 231 97 734.8 0 97 232 83 734.8 0 83 233 87 734.8 0 87 234 68 734.8 0 68 235 64 734.8 0 64 236 98 734.8 0 98 237 108 734.8 0 108 238 82 734.8 0 82 239 76 734.8 0 76 240 80 734.8 0 80 241 58 734.8 0 58 242 38 734.8 0 38 243 72 734.8 0 72 244 75 734.8 0 75 245 117 734.8 0 117 246 108 734.8 0 108 247 115 734.8 0 115 248 100 734.8 0 100 249 71 734.8 0 71 250 75 734.8 0 75 251 57 734.8 0 57 252 55 734.8 0 55 253 60 734.8 0 60 254 96 734.8 0 96 255 85 734.8 0 85 256 91 734.8 0 91 257 61 734.8 0 61 258 79 734.8 0 79 259 102 734.8 0 102 260 699 734.8 0 699 261 784 734.8 0 784 262 310 734.8 0 310 263 99 734.8 0 99 264 89 734.8 0 89 265 125 734.8 0 125 266 141 734.8 0 141 267 151 734.8 0 151 268 117 734.8 0 117 269 71 734.8 0 71 270 68 734.8 0 68 271 77 734.8 0 77 272 72 734.8 0 72 273 57 734.8 0 57 274 63 734.8 0 63 275 53 734.8 0 53 276 132 734.8 0 132 277 113 734.8 0 113 278 104 734.8 0 104 279 79 734.8 0 79 280 168 734.8 0 168 281 147 734.8 0 147 282 82 734.8 0 82 283 50 734.8 0 50 284 82 734.8 0 82 285 148 734.8 0 148 286 149 734.8 0 149 287 918 734.8 0 918 288 1391 734.8 0 1391 289 220 734.8 0 220 290 49 734.8 0 49 291 50 734.8 0 50 292 53 734.8 0 53 293 74 734.8 0 74 294 106 734.8 0 106 295 77 734.8 0 77 296 139 734.8 0 139 297 145 734.8 0 145 298 122 734.8 0 122 299 53 734.8 0 53 300 41 734.8 0 41 301 46 734.8 0 46 Finished in 13.81 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 291,583 Reads with adapters: 45,455 (15.6%) Reads that were too short: 122 (0.0%) Reads written (passing filters): 45,333 (15.5%) Total basepairs processed: 71,037,706 bp Total written (filtered): 11,097,676 bp (15.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 45455 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 3 0.0 3 0 0 0 1 2 18 26 0.0 3 0 0 18 7 1 19 389 0.0 3 0 355 16 11 7 20 33043 0.0 4 31125 1661 188 48 21 21 85 0.0 4 10 65 6 2 2 22 8 0.0 4 0 1 4 1 2 23 31 0.0 4 0 0 0 25 6 24 1 0.0 4 0 0 0 0 1 25 2 0.0 4 1 0 0 1 26 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 0 0 0 1 34 7 0.0 4 0 0 0 0 7 35 1 0.0 4 1 37 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 53 2 0.0 4 0 0 0 0 2 57 1 0.0 4 0 0 0 1 69 2 0.0 4 0 0 0 0 2 70 16 0.0 4 0 0 0 0 16 71 4 0.0 4 0 0 0 0 4 72 8662 0.0 4 0 0 0 0 8662 74 1 0.0 4 0 0 0 0 1 75 4 0.0 4 0 0 0 0 4 77 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 125 7 0.0 4 0 0 0 0 7 126 51 0.0 4 0 0 0 0 51 127 2974 0.0 4 0 0 0 0 2974 128 7 0.0 4 0 0 0 0 7 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 170 3 0.0 4 0 0 0 0 3 172 1 0.0 4 0 0 0 0 1 173 35 0.0 4 0 0 0 0 35 174 2 0.0 4 0 0 0 1 1 180 3 0.0 4 0 0 0 0 3 181 2 0.0 4 0 0 0 0 2 196 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 203 2 0.0 4 0 0 0 0 2 207 1 0.0 4 0 0 0 0 1 208 2 0.0 4 0 0 0 1 1 215 1 0.0 4 0 0 0 0 1 227 12 0.0 4 0 0 0 0 12 228 4 0.0 4 0 0 0 0 4 230 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 233 4 0.0 4 0 0 0 0 4 234 10 0.0 4 0 0 0 0 10 235 1 0.0 4 0 0 0 0 1 236 6 0.0 4 0 0 0 0 6 237 3 0.0 4 0 0 0 0 3 271 1 0.0 4 0 0 0 0 1 282 6 0.0 4 0 0 0 0 6 288 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 292 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.48 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 45,333 Reads with adapters: 2,670 (5.9%) Reads written (passing filters): 45,333 (100.0%) Total basepairs processed: 11,097,676 bp Total written (filtered): 10,917,516 bp (98.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2670 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 25.0% C: 4.2% G: 69.1% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 17 708.3 0 17 4 30 177.1 0 30 5 3 44.3 0 3 6 4 11.1 0 4 9 486 0.2 0 52 434 10 975 0.0 1 49 926 11 512 0.0 1 3 509 12 5 0.0 1 0 5 13 2 0.0 1 0 2 14 1 0.0 1 0 1 150 3 0.0 1 2 1 206 1 0.0 1 1 207 1 0.0 1 0 1 208 49 0.0 1 45 4 211 1 0.0 1 1 213 14 0.0 1 14 214 1 0.0 1 1 215 1 0.0 1 1 256 2 0.0 1 2 257 382 0.0 1 365 17 258 119 0.0 1 118 1 259 6 0.0 1 5 1 260 31 0.0 1 29 2 261 4 0.0 1 4 262 1 0.0 1 1 263 1 0.0 1 1 267 1 0.0 1 1 269 14 0.0 1 13 1 270 1 0.0 1 1 271 1 0.0 1 1 272 1 0.0 1 1