This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.29 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 287,867 (9.6%) Reads that were too short: 5,274 (0.2%) Reads written (passing filters): 282,593 (9.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 78,960,053 bp (8.7%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 287867 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2943 734.8 0 2943 7 5930 734.8 0 5930 8 240214 734.8 0 240214 9 566 734.8 0 566 10 65 734.8 0 65 11 35 734.8 0 35 12 41 734.8 0 41 13 40 734.8 0 40 14 37 734.8 0 37 15 42 734.8 0 42 16 78 734.8 0 78 17 42 734.8 0 42 18 50 734.8 0 50 19 105 734.8 0 105 20 96 734.8 0 96 21 92 734.8 0 92 22 27 734.8 0 27 23 37 734.8 0 37 24 31 734.8 0 31 25 36 734.8 0 36 26 60 734.8 0 60 27 42 734.8 0 42 28 24 734.8 0 24 29 46 734.8 0 46 30 52 734.8 0 52 31 50 734.8 0 50 32 34 734.8 0 34 33 40 734.8 0 40 34 33 734.8 0 33 35 41 734.8 0 41 36 40 734.8 0 40 37 41 734.8 0 41 38 44 734.8 0 44 39 54 734.8 0 54 40 69 734.8 0 69 41 785 734.8 0 785 42 51 734.8 0 51 43 26 734.8 0 26 44 47 734.8 0 47 45 40 734.8 0 40 46 33 734.8 0 33 47 43 734.8 0 43 48 44 734.8 0 44 49 47 734.8 0 47 50 46 734.8 0 46 51 43 734.8 0 43 52 43 734.8 0 43 53 37 734.8 0 37 54 35 734.8 0 35 55 40 734.8 0 40 56 41 734.8 0 41 57 39 734.8 0 39 58 57 734.8 0 57 59 31 734.8 0 31 60 38 734.8 0 38 61 45 734.8 0 45 62 26 734.8 0 26 63 44 734.8 0 44 64 50 734.8 0 50 65 50 734.8 0 50 66 43 734.8 0 43 67 53 734.8 0 53 68 45 734.8 0 45 69 29 734.8 0 29 70 40 734.8 0 40 71 58 734.8 0 58 72 37 734.8 0 37 73 43 734.8 0 43 74 49 734.8 0 49 75 49 734.8 0 49 76 200 734.8 0 200 77 102 734.8 0 102 78 50 734.8 0 50 79 51 734.8 0 51 80 44 734.8 0 44 81 43 734.8 0 43 82 48 734.8 0 48 83 60 734.8 0 60 84 64 734.8 0 64 85 44 734.8 0 44 86 43 734.8 0 43 87 50 734.8 0 50 88 53 734.8 0 53 89 36 734.8 0 36 90 45 734.8 0 45 91 62 734.8 0 62 92 73 734.8 0 73 93 249 734.8 0 249 94 170 734.8 0 170 95 65 734.8 0 65 96 192 734.8 0 192 97 253 734.8 0 253 98 129 734.8 0 129 99 58 734.8 0 58 100 43 734.8 0 43 101 54 734.8 0 54 102 41 734.8 0 41 103 53 734.8 0 53 104 30 734.8 0 30 105 39 734.8 0 39 106 40 734.8 0 40 107 44 734.8 0 44 108 45 734.8 0 45 109 55 734.8 0 55 110 48 734.8 0 48 111 33 734.8 0 33 112 31 734.8 0 31 113 37 734.8 0 37 114 77 734.8 0 77 115 69 734.8 0 69 116 77 734.8 0 77 117 113 734.8 0 113 118 62 734.8 0 62 119 67 734.8 0 67 120 83 734.8 0 83 121 52 734.8 0 52 122 59 734.8 0 59 123 108 734.8 0 108 124 72 734.8 0 72 125 114 734.8 0 114 126 121 734.8 0 121 127 204 734.8 0 204 128 230 734.8 0 230 129 543 734.8 0 543 130 12055 734.8 0 12055 131 4405 734.8 0 4405 132 569 734.8 0 569 133 167 734.8 0 167 134 43 734.8 0 43 135 35 734.8 0 35 136 40 734.8 0 40 137 40 734.8 0 40 138 48 734.8 0 48 139 33 734.8 0 33 140 37 734.8 0 37 141 37 734.8 0 37 142 38 734.8 0 38 143 69 734.8 0 69 144 84 734.8 0 84 145 38 734.8 0 38 146 28 734.8 0 28 147 29 734.8 0 29 148 41 734.8 0 41 149 43 734.8 0 43 150 38 734.8 0 38 151 39 734.8 0 39 152 48 734.8 0 48 153 39 734.8 0 39 154 49 734.8 0 49 155 32 734.8 0 32 156 37 734.8 0 37 157 40 734.8 0 40 158 47 734.8 0 47 159 51 734.8 0 51 160 40 734.8 0 40 161 54 734.8 0 54 162 62 734.8 0 62 163 34 734.8 0 34 164 46 734.8 0 46 165 64 734.8 0 64 166 60 734.8 0 60 167 115 734.8 0 115 168 147 734.8 0 147 169 2279 734.8 0 2279 170 1607 734.8 0 1607 171 428 734.8 0 428 172 35 734.8 0 35 173 37 734.8 0 37 174 55 734.8 0 55 175 29 734.8 0 29 176 31 734.8 0 31 177 20 734.8 0 20 178 24 734.8 0 24 179 48 734.8 0 48 180 45 734.8 0 45 181 37 734.8 0 37 182 34 734.8 0 34 183 24 734.8 0 24 184 44 734.8 0 44 185 47 734.8 0 47 186 40 734.8 0 40 187 28 734.8 0 28 188 31 734.8 0 31 189 43 734.8 0 43 190 43 734.8 0 43 191 49 734.8 0 49 192 66 734.8 0 66 193 59 734.8 0 59 194 34 734.8 0 34 195 26 734.8 0 26 196 28 734.8 0 28 197 40 734.8 0 40 198 35 734.8 0 35 199 25 734.8 0 25 200 31 734.8 0 31 201 38 734.8 0 38 202 38 734.8 0 38 203 84 734.8 0 84 204 78 734.8 0 78 205 53 734.8 0 53 206 41 734.8 0 41 207 31 734.8 0 31 208 35 734.8 0 35 209 59 734.8 0 59 210 58 734.8 0 58 211 60 734.8 0 60 212 52 734.8 0 52 213 34 734.8 0 34 214 42 734.8 0 42 215 71 734.8 0 71 216 50 734.8 0 50 217 43 734.8 0 43 218 47 734.8 0 47 219 35 734.8 0 35 220 42 734.8 0 42 221 43 734.8 0 43 222 143 734.8 0 143 223 137 734.8 0 137 224 57 734.8 0 57 225 42 734.8 0 42 226 52 734.8 0 52 227 39 734.8 0 39 228 45 734.8 0 45 229 33 734.8 0 33 230 39 734.8 0 39 231 62 734.8 0 62 232 97 734.8 0 97 233 62 734.8 0 62 234 51 734.8 0 51 235 95 734.8 0 95 236 93 734.8 0 93 237 376 734.8 0 376 238 52 734.8 0 52 239 42 734.8 0 42 240 30 734.8 0 30 241 46 734.8 0 46 242 46 734.8 0 46 243 26 734.8 0 26 244 30 734.8 0 30 245 34 734.8 0 34 246 52 734.8 0 52 247 32 734.8 0 32 248 21 734.8 0 21 249 33 734.8 0 33 250 37 734.8 0 37 251 45 734.8 0 45 252 32 734.8 0 32 253 69 734.8 0 69 254 54 734.8 0 54 255 53 734.8 0 53 256 66 734.8 0 66 257 57 734.8 0 57 258 33 734.8 0 33 259 26 734.8 0 26 260 31 734.8 0 31 261 43 734.8 0 43 262 47 734.8 0 47 263 34 734.8 0 34 264 50 734.8 0 50 265 26 734.8 0 26 266 49 734.8 0 49 267 26 734.8 0 26 268 37 734.8 0 37 269 36 734.8 0 36 270 45 734.8 0 45 271 127 734.8 0 127 272 164 734.8 0 164 273 141 734.8 0 141 274 62 734.8 0 62 275 23 734.8 0 23 276 43 734.8 0 43 277 37 734.8 0 37 278 25 734.8 0 25 279 33 734.8 0 33 280 30 734.8 0 30 281 28 734.8 0 28 282 29 734.8 0 29 283 33 734.8 0 33 284 34 734.8 0 34 285 27 734.8 0 27 286 36 734.8 0 36 287 34 734.8 0 34 288 33 734.8 0 33 289 36 734.8 0 36 290 34 734.8 0 34 291 44 734.8 0 44 292 34 734.8 0 34 293 52 734.8 0 52 294 29 734.8 0 29 295 25 734.8 0 25 296 102 734.8 0 102 297 123 734.8 0 123 298 29 734.8 0 29 299 23 734.8 0 23 300 21 734.8 0 21 301 24 734.8 0 24 Finished in 12.01 s (42 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 282,593 Reads with adapters: 245,715 (87.0%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 245,706 (86.9%) Total basepairs processed: 78,960,053 bp Total written (filtered): 67,079,753 bp (85.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 245715 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 0 6 18 101 0.0 3 0 0 78 18 5 19 2456 0.0 3 0 2252 88 72 44 20 241917 0.0 4 235336 5507 727 227 120 21 661 0.0 4 96 541 19 4 1 22 66 0.0 4 7 3 39 2 15 23 418 0.0 4 87 5 3 259 64 24 9 0.0 4 2 0 0 1 6 25 1 0.0 4 0 0 1 26 2 0.0 4 0 2 27 2 0.0 4 0 0 1 1 28 1 0.0 4 0 0 1 29 1 0.0 4 1 33 1 0.0 4 1 36 2 0.0 4 2 41 14 0.0 4 0 0 0 0 14 42 3 0.0 4 2 0 0 0 1 108 1 0.0 4 0 0 0 1 110 2 0.0 4 0 0 0 1 1 111 25 0.0 4 0 0 0 20 5 112 2 0.0 4 0 0 0 0 2 118 1 0.0 4 0 0 0 0 1 127 17 0.0 4 0 0 0 0 17 162 2 0.0 4 0 0 0 0 2 184 3 0.0 4 0 0 0 0 3 197 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 8.55 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 245,706 Reads with adapters: 24,714 (10.1%) Reads written (passing filters): 245,706 (100.0%) Total basepairs processed: 67,079,753 bp Total written (filtered): 66,479,718 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 24714 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.1% C: 3.2% G: 87.4% T: 1.2% none/other: 4.2% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 15 3839.2 0 15 4 11 959.8 0 11 5 1 239.9 0 1 6 1 60.0 0 1 7 1 15.0 0 1 9 38 0.9 0 3 35 10 2836 0.2 1 171 2665 11 20386 0.1 1 112 20274 12 77 0.0 1 3 74 13 5 0.0 1 0 5 14 5 0.0 1 0 5 15 3 0.0 1 0 3 23 2 0.0 1 1 1 25 1 0.0 1 1 73 1 0.0 1 1 108 1 0.0 1 1 149 1 0.0 1 1 151 22 0.0 1 17 5 159 1 0.0 1 1 201 1 0.0 1 1 209 173 0.0 1 152 21 210 7 0.0 1 7 211 26 0.0 1 23 3 212 1 0.0 1 1 221 2 0.0 1 0 2 224 12 0.0 1 12 228 1 0.0 1 1 229 1 0.0 1 1 257 3 0.0 1 3 258 1 0.0 1 1 259 7 0.0 1 6 1 260 20 0.0 1 18 2 261 8 0.0 1 8 271 13 0.0 1 13 272 3 0.0 1 3 273 998 0.0 1 968 30 274 17 0.0 1 14 3 275 12 0.0 1 9 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.