This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.45 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 294,299 (9.8%) Reads that were too short: 31,619 (1.1%) Reads written (passing filters): 262,680 (8.7%) Total basepairs processed: 905,907,359 bp Total written (filtered): 75,620,127 bp (8.3%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 294299 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1330 734.8 0 1330 7 2197 734.8 0 2197 8 242041 734.8 0 242041 9 4276 734.8 0 4276 10 60 734.8 0 60 11 47 734.8 0 47 12 62 734.8 0 62 13 67 734.8 0 67 14 45 734.8 0 45 15 65 734.8 0 65 16 81 734.8 0 81 17 62 734.8 0 62 18 51 734.8 0 51 19 52 734.8 0 52 20 56 734.8 0 56 21 66 734.8 0 66 22 53 734.8 0 53 23 56 734.8 0 56 24 66 734.8 0 66 25 50 734.8 0 50 26 41 734.8 0 41 27 55 734.8 0 55 28 54 734.8 0 54 29 61 734.8 0 61 30 62 734.8 0 62 31 45 734.8 0 45 32 50 734.8 0 50 33 47 734.8 0 47 34 49 734.8 0 49 35 57 734.8 0 57 36 61 734.8 0 61 37 47 734.8 0 47 38 60 734.8 0 60 39 33 734.8 0 33 40 50 734.8 0 50 41 51 734.8 0 51 42 55 734.8 0 55 43 53 734.8 0 53 44 69 734.8 0 69 45 97 734.8 0 97 46 66 734.8 0 66 47 72 734.8 0 72 48 78 734.8 0 78 49 50 734.8 0 50 50 53 734.8 0 53 51 44 734.8 0 44 52 54 734.8 0 54 53 44 734.8 0 44 54 64 734.8 0 64 55 50 734.8 0 50 56 52 734.8 0 52 57 48 734.8 0 48 58 58 734.8 0 58 59 101 734.8 0 101 60 126 734.8 0 126 61 76 734.8 0 76 62 55 734.8 0 55 63 68 734.8 0 68 64 52 734.8 0 52 65 49 734.8 0 49 66 40 734.8 0 40 67 73 734.8 0 73 68 65 734.8 0 65 69 45 734.8 0 45 70 100 734.8 0 100 71 58 734.8 0 58 72 68 734.8 0 68 73 59 734.8 0 59 74 46 734.8 0 46 75 53 734.8 0 53 76 55 734.8 0 55 77 78 734.8 0 78 78 76 734.8 0 76 79 45 734.8 0 45 80 64 734.8 0 64 81 65 734.8 0 65 82 45 734.8 0 45 83 62 734.8 0 62 84 43 734.8 0 43 85 61 734.8 0 61 86 42 734.8 0 42 87 57 734.8 0 57 88 55 734.8 0 55 89 39 734.8 0 39 90 52 734.8 0 52 91 58 734.8 0 58 92 51 734.8 0 51 93 62 734.8 0 62 94 77 734.8 0 77 95 70 734.8 0 70 96 62 734.8 0 62 97 59 734.8 0 59 98 138 734.8 0 138 99 81 734.8 0 81 100 65 734.8 0 65 101 48 734.8 0 48 102 53 734.8 0 53 103 62 734.8 0 62 104 69 734.8 0 69 105 41 734.8 0 41 106 67 734.8 0 67 107 51 734.8 0 51 108 46 734.8 0 46 109 50 734.8 0 50 110 59 734.8 0 59 111 57 734.8 0 57 112 63 734.8 0 63 113 91 734.8 0 91 114 68 734.8 0 68 115 62 734.8 0 62 116 47 734.8 0 47 117 64 734.8 0 64 118 64 734.8 0 64 119 63 734.8 0 63 120 61 734.8 0 61 121 57 734.8 0 57 122 47 734.8 0 47 123 55 734.8 0 55 124 65 734.8 0 65 125 60 734.8 0 60 126 60 734.8 0 60 127 69 734.8 0 69 128 88 734.8 0 88 129 67 734.8 0 67 130 100 734.8 0 100 131 89 734.8 0 89 132 102 734.8 0 102 133 82 734.8 0 82 134 90 734.8 0 90 135 89 734.8 0 89 136 61 734.8 0 61 137 62 734.8 0 62 138 106 734.8 0 106 139 82 734.8 0 82 140 100 734.8 0 100 141 71 734.8 0 71 142 70 734.8 0 70 143 89 734.8 0 89 144 95 734.8 0 95 145 181 734.8 0 181 146 63 734.8 0 63 147 57 734.8 0 57 148 63 734.8 0 63 149 54 734.8 0 54 150 52 734.8 0 52 151 68 734.8 0 68 152 69 734.8 0 69 153 56 734.8 0 56 154 42 734.8 0 42 155 68 734.8 0 68 156 83 734.8 0 83 157 113 734.8 0 113 158 78 734.8 0 78 159 72 734.8 0 72 160 74 734.8 0 74 161 53 734.8 0 53 162 49 734.8 0 49 163 37 734.8 0 37 164 67 734.8 0 67 165 53 734.8 0 53 166 47 734.8 0 47 167 59 734.8 0 59 168 54 734.8 0 54 169 48 734.8 0 48 170 47 734.8 0 47 171 73 734.8 0 73 172 42 734.8 0 42 173 54 734.8 0 54 174 55 734.8 0 55 175 59 734.8 0 59 176 52 734.8 0 52 177 56 734.8 0 56 178 51 734.8 0 51 179 56 734.8 0 56 180 62 734.8 0 62 181 59 734.8 0 59 182 53 734.8 0 53 183 54 734.8 0 54 184 67 734.8 0 67 185 66 734.8 0 66 186 56 734.8 0 56 187 76 734.8 0 76 188 49 734.8 0 49 189 54 734.8 0 54 190 76 734.8 0 76 191 241 734.8 0 241 192 187 734.8 0 187 193 92 734.8 0 92 194 69 734.8 0 69 195 42 734.8 0 42 196 90 734.8 0 90 197 258 734.8 0 258 198 240 734.8 0 240 199 92 734.8 0 92 200 63 734.8 0 63 201 78 734.8 0 78 202 39 734.8 0 39 203 42 734.8 0 42 204 64 734.8 0 64 205 93 734.8 0 93 206 58 734.8 0 58 207 58 734.8 0 58 208 51 734.8 0 51 209 60 734.8 0 60 210 57 734.8 0 57 211 60 734.8 0 60 212 62 734.8 0 62 213 64 734.8 0 64 214 60 734.8 0 60 215 53 734.8 0 53 216 51 734.8 0 51 217 51 734.8 0 51 218 78 734.8 0 78 219 83 734.8 0 83 220 59 734.8 0 59 221 89 734.8 0 89 222 71 734.8 0 71 223 100 734.8 0 100 224 368 734.8 0 368 225 76 734.8 0 76 226 65 734.8 0 65 227 45 734.8 0 45 228 54 734.8 0 54 229 63 734.8 0 63 230 68 734.8 0 68 231 76 734.8 0 76 232 107 734.8 0 107 233 96 734.8 0 96 234 94 734.8 0 94 235 56 734.8 0 56 236 75 734.8 0 75 237 85 734.8 0 85 238 87 734.8 0 87 239 93 734.8 0 93 240 98 734.8 0 98 241 77 734.8 0 77 242 62 734.8 0 62 243 54 734.8 0 54 244 65 734.8 0 65 245 95 734.8 0 95 246 84 734.8 0 84 247 53 734.8 0 53 248 52 734.8 0 52 249 53 734.8 0 53 250 58 734.8 0 58 251 46 734.8 0 46 252 41 734.8 0 41 253 62 734.8 0 62 254 65 734.8 0 65 255 86 734.8 0 86 256 134 734.8 0 134 257 144 734.8 0 144 258 109 734.8 0 109 259 82 734.8 0 82 260 86 734.8 0 86 261 88 734.8 0 88 262 79 734.8 0 79 263 76 734.8 0 76 264 99 734.8 0 99 265 208 734.8 0 208 266 290 734.8 0 290 267 118 734.8 0 118 268 189 734.8 0 189 269 54 734.8 0 54 270 47 734.8 0 47 271 59 734.8 0 59 272 72 734.8 0 72 273 109 734.8 0 109 274 78 734.8 0 78 275 51 734.8 0 51 276 59 734.8 0 59 277 69 734.8 0 69 278 70 734.8 0 70 279 53 734.8 0 53 280 109 734.8 0 109 281 165 734.8 0 165 282 120 734.8 0 120 283 126 734.8 0 126 284 164 734.8 0 164 285 222 734.8 0 222 286 178 734.8 0 178 287 142 734.8 0 142 288 251 734.8 0 251 289 246 734.8 0 246 290 323 734.8 0 323 291 480 734.8 0 480 292 545 734.8 0 545 293 643 734.8 0 643 294 518 734.8 0 518 295 610 734.8 0 610 296 637 734.8 0 637 297 779 734.8 0 779 298 4809 734.8 0 4809 299 4477 734.8 0 4477 300 8365 734.8 0 8365 301 1325 734.8 0 1325 Finished in 10.69 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 262,680 Reads with adapters: 245,144 (93.3%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 245,138 (93.3%) Total basepairs processed: 75,620,127 bp Total written (filtered): 67,661,512 bp (89.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 245144 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 50 0.0 3 0 0 47 3 16 1130 0.0 3 0 1106 24 17 243098 0.0 3 240015 3039 38 6 18 579 0.0 3 26 530 16 7 19 90 0.0 3 1 1 78 10 20 165 0.0 3 1 1 1 162 22 1 0.0 3 0 0 0 1 24 3 0.0 3 1 0 2 25 1 0.0 3 1 27 1 0.0 3 1 29 1 0.0 3 1 30 2 0.0 3 2 33 1 0.0 3 0 0 0 1 34 1 0.0 3 1 35 1 0.0 3 1 36 2 0.0 3 2 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 8.68 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 245,138 Reads with adapters: 18,319 (7.5%) Reads written (passing filters): 245,138 (100.0%) Total basepairs processed: 67,661,512 bp Total written (filtered): 67,606,496 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 18319 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 24.5% C: 1.9% G: 72.7% T: 1.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 18267 3830.3 0 18267 4 47 957.6 0 47 5 4 239.4 0 4 7 1 15.0 0 1