This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.95 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 695,132 (23.1%) Reads that were too short: 545,036 (18.1%) Reads written (passing filters): 150,096 (5.0%) Total basepairs processed: 905,907,359 bp Total written (filtered): 36,163,305 bp (4.0%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 695132 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 710 734.8 0 710 7 82993 734.8 0 82993 8 182 734.8 0 182 9 3877 734.8 0 3877 10 40 734.8 0 40 11 28 734.8 0 28 12 38 734.8 0 38 13 41 734.8 0 41 14 40 734.8 0 40 15 36 734.8 0 36 16 27 734.8 0 27 17 36 734.8 0 36 18 42 734.8 0 42 19 38 734.8 0 38 20 27 734.8 0 27 21 35 734.8 0 35 22 32 734.8 0 32 23 28 734.8 0 28 24 32 734.8 0 32 25 29 734.8 0 29 26 30 734.8 0 30 27 27 734.8 0 27 28 45 734.8 0 45 29 38 734.8 0 38 30 105 734.8 0 105 31 479 734.8 0 479 32 35 734.8 0 35 33 45 734.8 0 45 34 31 734.8 0 31 35 38 734.8 0 38 36 52 734.8 0 52 37 86 734.8 0 86 38 64 734.8 0 64 39 44 734.8 0 44 40 44 734.8 0 44 41 55 734.8 0 55 42 63 734.8 0 63 43 37 734.8 0 37 44 36 734.8 0 36 45 37 734.8 0 37 46 37 734.8 0 37 47 36 734.8 0 36 48 35 734.8 0 35 49 28 734.8 0 28 50 30 734.8 0 30 51 29 734.8 0 29 52 31 734.8 0 31 53 41 734.8 0 41 54 25 734.8 0 25 55 25 734.8 0 25 56 30 734.8 0 30 57 22 734.8 0 22 58 29 734.8 0 29 59 41 734.8 0 41 60 39 734.8 0 39 61 24 734.8 0 24 62 26 734.8 0 26 63 19 734.8 0 19 64 21 734.8 0 21 65 28 734.8 0 28 66 31 734.8 0 31 67 31 734.8 0 31 68 49 734.8 0 49 69 58 734.8 0 58 70 113 734.8 0 113 71 47 734.8 0 47 72 65 734.8 0 65 73 119 734.8 0 119 74 55 734.8 0 55 75 33 734.8 0 33 76 41 734.8 0 41 77 161 734.8 0 161 78 495 734.8 0 495 79 139 734.8 0 139 80 499 734.8 0 499 81 355 734.8 0 355 82 372 734.8 0 372 83 184 734.8 0 184 84 216 734.8 0 216 85 129 734.8 0 129 86 148 734.8 0 148 87 206 734.8 0 206 88 278 734.8 0 278 89 252 734.8 0 252 90 458 734.8 0 458 91 439 734.8 0 439 92 577 734.8 0 577 93 736 734.8 0 736 94 487 734.8 0 487 95 433 734.8 0 433 96 406 734.8 0 406 97 928 734.8 0 928 98 2004 734.8 0 2004 99 827 734.8 0 827 100 449 734.8 0 449 101 474 734.8 0 474 102 187 734.8 0 187 103 159 734.8 0 159 104 81 734.8 0 81 105 189 734.8 0 189 106 957 734.8 0 957 107 697 734.8 0 697 108 419 734.8 0 419 109 365 734.8 0 365 110 543 734.8 0 543 111 561 734.8 0 561 112 457 734.8 0 457 113 437 734.8 0 437 114 378 734.8 0 378 115 312 734.8 0 312 116 192 734.8 0 192 117 214 734.8 0 214 118 248 734.8 0 248 119 713 734.8 0 713 120 704 734.8 0 704 121 476 734.8 0 476 122 321 734.8 0 321 123 735 734.8 0 735 124 542 734.8 0 542 125 374 734.8 0 374 126 369 734.8 0 369 127 290 734.8 0 290 128 521 734.8 0 521 129 611 734.8 0 611 130 439 734.8 0 439 131 406 734.8 0 406 132 778 734.8 0 778 133 472 734.8 0 472 134 448 734.8 0 448 135 327 734.8 0 327 136 292 734.8 0 292 137 281 734.8 0 281 138 404 734.8 0 404 139 1456 734.8 0 1456 140 1096 734.8 0 1096 141 661 734.8 0 661 142 255 734.8 0 255 143 291 734.8 0 291 144 262 734.8 0 262 145 326 734.8 0 326 146 453 734.8 0 453 147 315 734.8 0 315 148 340 734.8 0 340 149 256 734.8 0 256 150 310 734.8 0 310 151 236 734.8 0 236 152 257 734.8 0 257 153 311 734.8 0 311 154 341 734.8 0 341 155 438 734.8 0 438 156 358 734.8 0 358 157 366 734.8 0 366 158 429 734.8 0 429 159 2120 734.8 0 2120 160 4243 734.8 0 4243 161 1893 734.8 0 1893 162 257 734.8 0 257 163 344 734.8 0 344 164 331 734.8 0 331 165 465 734.8 0 465 166 344 734.8 0 344 167 380 734.8 0 380 168 396 734.8 0 396 169 245 734.8 0 245 170 200 734.8 0 200 171 215 734.8 0 215 172 244 734.8 0 244 173 446 734.8 0 446 174 695 734.8 0 695 175 253 734.8 0 253 176 280 734.8 0 280 177 264 734.8 0 264 178 280 734.8 0 280 179 267 734.8 0 267 180 366 734.8 0 366 181 352 734.8 0 352 182 264 734.8 0 264 183 361 734.8 0 361 184 411 734.8 0 411 185 485 734.8 0 485 186 315 734.8 0 315 187 329 734.8 0 329 188 310 734.8 0 310 189 361 734.8 0 361 190 298 734.8 0 298 191 353 734.8 0 353 192 457 734.8 0 457 193 488 734.8 0 488 194 548 734.8 0 548 195 573 734.8 0 573 196 427 734.8 0 427 197 263 734.8 0 263 198 248 734.8 0 248 199 229 734.8 0 229 200 647 734.8 0 647 201 601 734.8 0 601 202 379 734.8 0 379 203 542 734.8 0 542 204 550 734.8 0 550 205 537 734.8 0 537 206 778 734.8 0 778 207 1143 734.8 0 1143 208 949 734.8 0 949 209 859 734.8 0 859 210 603 734.8 0 603 211 694 734.8 0 694 212 696 734.8 0 696 213 527 734.8 0 527 214 392 734.8 0 392 215 361 734.8 0 361 216 294 734.8 0 294 217 311 734.8 0 311 218 766 734.8 0 766 219 639 734.8 0 639 220 611 734.8 0 611 221 752 734.8 0 752 222 420 734.8 0 420 223 441 734.8 0 441 224 604 734.8 0 604 225 432 734.8 0 432 226 296 734.8 0 296 227 391 734.8 0 391 228 896 734.8 0 896 229 513 734.8 0 513 230 492 734.8 0 492 231 511 734.8 0 511 232 1213 734.8 0 1213 233 1126 734.8 0 1126 234 751 734.8 0 751 235 526 734.8 0 526 236 456 734.8 0 456 237 655 734.8 0 655 238 1332 734.8 0 1332 239 415 734.8 0 415 240 752 734.8 0 752 241 800 734.8 0 800 242 889 734.8 0 889 243 1254 734.8 0 1254 244 745 734.8 0 745 245 606 734.8 0 606 246 271 734.8 0 271 247 386 734.8 0 386 248 718 734.8 0 718 249 1343 734.8 0 1343 250 2021 734.8 0 2021 251 2624 734.8 0 2624 252 3717 734.8 0 3717 253 7515 734.8 0 7515 254 15200 734.8 0 15200 255 220635 734.8 0 220635 256 202748 734.8 0 202748 257 43411 734.8 0 43411 258 663 734.8 0 663 259 420 734.8 0 420 260 607 734.8 0 607 261 615 734.8 0 615 262 852 734.8 0 852 263 496 734.8 0 496 264 211 734.8 0 211 265 276 734.8 0 276 266 257 734.8 0 257 267 167 734.8 0 167 268 218 734.8 0 218 269 364 734.8 0 364 270 333 734.8 0 333 271 339 734.8 0 339 272 307 734.8 0 307 273 335 734.8 0 335 274 247 734.8 0 247 275 199 734.8 0 199 276 188 734.8 0 188 277 241 734.8 0 241 278 221 734.8 0 221 279 249 734.8 0 249 280 290 734.8 0 290 281 380 734.8 0 380 282 576 734.8 0 576 283 473 734.8 0 473 284 523 734.8 0 523 285 187 734.8 0 187 286 103 734.8 0 103 287 108 734.8 0 108 288 197 734.8 0 197 289 380 734.8 0 380 290 290 734.8 0 290 291 236 734.8 0 236 292 220 734.8 0 220 293 198 734.8 0 198 294 164 734.8 0 164 295 332 734.8 0 332 296 478 734.8 0 478 297 641 734.8 0 641 298 731 734.8 0 731 299 502 734.8 0 502 300 412 734.8 0 412 301 322 734.8 0 322 Finished in 6.07 s (40 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 150,096 Reads with adapters: 3,953 (2.6%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 3,934 (2.6%) Total basepairs processed: 36,163,305 bp Total written (filtered): 1,079,382 bp (3.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3953 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 43 0.0 3 0 41 2 17 3847 0.0 3 3772 70 5 18 17 0.0 3 0 14 1 2 19 4 0.0 3 0 0 2 2 20 3 0.0 3 0 0 0 3 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 1 1 48 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 1 114 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 144 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 214 1 0.0 3 0 0 1 281 4 0.0 3 0 0 0 4 282 1 0.0 3 0 0 0 1 283 2 0.0 3 0 0 0 2 288 2 0.0 3 0 0 1 1 289 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.13 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 3,934 Reads with adapters: 315 (8.0%) Reads written (passing filters): 3,934 (100.0%) Total basepairs processed: 1,079,382 bp Total written (filtered): 1,078,437 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 315 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 65.7% C: 6.0% G: 27.9% T: 0.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 315 61.5 0 315