This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.29 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 60,586 (2.0%) Reads that were too short: 15,994 (0.5%) Reads written (passing filters): 44,592 (1.5%) Total basepairs processed: 905,907,359 bp Total written (filtered): 11,718,440 bp (1.3%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 60586 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1079 734.8 0 1079 7 1873 734.8 0 1873 8 28480 734.8 0 28480 9 79 734.8 0 79 10 51 734.8 0 51 11 34 734.8 0 34 12 25 734.8 0 25 13 26 734.8 0 26 14 39 734.8 0 39 15 30 734.8 0 30 16 29 734.8 0 29 17 174 734.8 0 174 18 62 734.8 0 62 19 55 734.8 0 55 20 26 734.8 0 26 21 29 734.8 0 29 22 22 734.8 0 22 23 24 734.8 0 24 24 26 734.8 0 26 25 30 734.8 0 30 26 33 734.8 0 33 27 19 734.8 0 19 28 26 734.8 0 26 29 44 734.8 0 44 30 41 734.8 0 41 31 22 734.8 0 22 32 25 734.8 0 25 33 42 734.8 0 42 34 24 734.8 0 24 35 36 734.8 0 36 36 38 734.8 0 38 37 25 734.8 0 25 38 32 734.8 0 32 39 31 734.8 0 31 40 36 734.8 0 36 41 33 734.8 0 33 42 54 734.8 0 54 43 42 734.8 0 42 44 21 734.8 0 21 45 23 734.8 0 23 46 40 734.8 0 40 47 43 734.8 0 43 48 41 734.8 0 41 49 48 734.8 0 48 50 22 734.8 0 22 51 22 734.8 0 22 52 28 734.8 0 28 53 59 734.8 0 59 54 28 734.8 0 28 55 43 734.8 0 43 56 42 734.8 0 42 57 48 734.8 0 48 58 30 734.8 0 30 59 34 734.8 0 34 60 43 734.8 0 43 61 39 734.8 0 39 62 37 734.8 0 37 63 27 734.8 0 27 64 27 734.8 0 27 65 32 734.8 0 32 66 38 734.8 0 38 67 48 734.8 0 48 68 36 734.8 0 36 69 19 734.8 0 19 70 31 734.8 0 31 71 46 734.8 0 46 72 49 734.8 0 49 73 453 734.8 0 453 74 438 734.8 0 438 75 80 734.8 0 80 76 51 734.8 0 51 77 916 734.8 0 916 78 752 734.8 0 752 79 197 734.8 0 197 80 34 734.8 0 34 81 26 734.8 0 26 82 35 734.8 0 35 83 25 734.8 0 25 84 29 734.8 0 29 85 34 734.8 0 34 86 38 734.8 0 38 87 28 734.8 0 28 88 28 734.8 0 28 89 32 734.8 0 32 90 33 734.8 0 33 91 31 734.8 0 31 92 39 734.8 0 39 93 40 734.8 0 40 94 28 734.8 0 28 95 18 734.8 0 18 96 40 734.8 0 40 97 23 734.8 0 23 98 31 734.8 0 31 99 39 734.8 0 39 100 26 734.8 0 26 101 20 734.8 0 20 102 16 734.8 0 16 103 25 734.8 0 25 104 23 734.8 0 23 105 28 734.8 0 28 106 121 734.8 0 121 107 163 734.8 0 163 108 47 734.8 0 47 109 28 734.8 0 28 110 35 734.8 0 35 111 31 734.8 0 31 112 35 734.8 0 35 113 36 734.8 0 36 114 51 734.8 0 51 115 46 734.8 0 46 116 33 734.8 0 33 117 31 734.8 0 31 118 31 734.8 0 31 119 36 734.8 0 36 120 43 734.8 0 43 121 34 734.8 0 34 122 28 734.8 0 28 123 31 734.8 0 31 124 25 734.8 0 25 125 42 734.8 0 42 126 44 734.8 0 44 127 432 734.8 0 432 128 93 734.8 0 93 129 143 734.8 0 143 130 55 734.8 0 55 131 43 734.8 0 43 132 57 734.8 0 57 133 48 734.8 0 48 134 125 734.8 0 125 135 58 734.8 0 58 136 68 734.8 0 68 137 49 734.8 0 49 138 40 734.8 0 40 139 78 734.8 0 78 140 58 734.8 0 58 141 46 734.8 0 46 142 38 734.8 0 38 143 44 734.8 0 44 144 74 734.8 0 74 145 65 734.8 0 65 146 912 734.8 0 912 147 684 734.8 0 684 148 123 734.8 0 123 149 38 734.8 0 38 150 31 734.8 0 31 151 19 734.8 0 19 152 32 734.8 0 32 153 31 734.8 0 31 154 28 734.8 0 28 155 64 734.8 0 64 156 116 734.8 0 116 157 28 734.8 0 28 158 32 734.8 0 32 159 53 734.8 0 53 160 85 734.8 0 85 161 31 734.8 0 31 162 32 734.8 0 32 163 33 734.8 0 33 164 81 734.8 0 81 165 110 734.8 0 110 166 85 734.8 0 85 167 65 734.8 0 65 168 66 734.8 0 66 169 39 734.8 0 39 170 51 734.8 0 51 171 29 734.8 0 29 172 39 734.8 0 39 173 27 734.8 0 27 174 35 734.8 0 35 175 28 734.8 0 28 176 23 734.8 0 23 177 29 734.8 0 29 178 27 734.8 0 27 179 33 734.8 0 33 180 46 734.8 0 46 181 39 734.8 0 39 182 28 734.8 0 28 183 28 734.8 0 28 184 171 734.8 0 171 185 162 734.8 0 162 186 76 734.8 0 76 187 19 734.8 0 19 188 29 734.8 0 29 189 21 734.8 0 21 190 29 734.8 0 29 191 35 734.8 0 35 192 39 734.8 0 39 193 38 734.8 0 38 194 38 734.8 0 38 195 185 734.8 0 185 196 151 734.8 0 151 197 89 734.8 0 89 198 44 734.8 0 44 199 29 734.8 0 29 200 48 734.8 0 48 201 28 734.8 0 28 202 39 734.8 0 39 203 29 734.8 0 29 204 145 734.8 0 145 205 59 734.8 0 59 206 45 734.8 0 45 207 34 734.8 0 34 208 42 734.8 0 42 209 38 734.8 0 38 210 25 734.8 0 25 211 31 734.8 0 31 212 32 734.8 0 32 213 45 734.8 0 45 214 50 734.8 0 50 215 57 734.8 0 57 216 91 734.8 0 91 217 72 734.8 0 72 218 39 734.8 0 39 219 60 734.8 0 60 220 45 734.8 0 45 221 39 734.8 0 39 222 37 734.8 0 37 223 30 734.8 0 30 224 39 734.8 0 39 225 48 734.8 0 48 226 68 734.8 0 68 227 27 734.8 0 27 228 34 734.8 0 34 229 52 734.8 0 52 230 42 734.8 0 42 231 61 734.8 0 61 232 62 734.8 0 62 233 110 734.8 0 110 234 122 734.8 0 122 235 216 734.8 0 216 236 1700 734.8 0 1700 237 6950 734.8 0 6950 238 660 734.8 0 660 239 49 734.8 0 49 240 28 734.8 0 28 241 41 734.8 0 41 242 28 734.8 0 28 243 18 734.8 0 18 244 44 734.8 0 44 245 41 734.8 0 41 246 86 734.8 0 86 247 70 734.8 0 70 248 35 734.8 0 35 249 30 734.8 0 30 250 21 734.8 0 21 251 21 734.8 0 21 252 81 734.8 0 81 253 81 734.8 0 81 254 121 734.8 0 121 255 64 734.8 0 64 256 49 734.8 0 49 257 33 734.8 0 33 258 28 734.8 0 28 259 25 734.8 0 25 260 20 734.8 0 20 261 18 734.8 0 18 262 15 734.8 0 15 263 23 734.8 0 23 264 34 734.8 0 34 265 35 734.8 0 35 266 42 734.8 0 42 267 39 734.8 0 39 268 32 734.8 0 32 269 25 734.8 0 25 270 28 734.8 0 28 271 29 734.8 0 29 272 41 734.8 0 41 273 36 734.8 0 36 274 33 734.8 0 33 275 24 734.8 0 24 276 72 734.8 0 72 277 131 734.8 0 131 278 224 734.8 0 224 279 177 734.8 0 177 280 196 734.8 0 196 281 671 734.8 0 671 282 332 734.8 0 332 283 79 734.8 0 79 284 242 734.8 0 242 285 200 734.8 0 200 286 67 734.8 0 67 287 20 734.8 0 20 288 28 734.8 0 28 289 26 734.8 0 26 290 23 734.8 0 23 291 28 734.8 0 28 292 40 734.8 0 40 293 54 734.8 0 54 294 39 734.8 0 39 295 41 734.8 0 41 296 151 734.8 0 151 297 205 734.8 0 205 298 93 734.8 0 93 299 27 734.8 0 27 300 52 734.8 0 52 301 33 734.8 0 33 Finished in 2.12 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 44,592 Reads with adapters: 29,873 (67.0%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 29,869 (67.0%) Total basepairs processed: 11,718,440 bp Total written (filtered): 8,149,896 bp (69.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 29873 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 15 0.0 3 0 0 14 0 1 19 427 0.0 3 0 401 11 11 4 20 28626 0.0 4 27712 750 115 34 15 21 90 0.0 4 15 66 3 2 4 22 620 0.0 4 577 27 14 1 1 23 33 0.0 4 0 2 0 24 7 25 3 0.0 4 1 0 0 1 1 57 20 0.0 4 0 0 0 0 20 61 8 0.0 4 0 0 0 0 8 74 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 0 0 0 2 109 6 0.0 4 0 0 0 6 110 1 0.0 4 0 0 0 0 1 111 15 0.0 4 0 0 0 14 1 113 1 0.0 4 0 0 0 1 142 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.03 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 29,869 Reads with adapters: 2,546 (8.5%) Reads written (passing filters): 29,869 (100.0%) Total basepairs processed: 8,149,896 bp Total written (filtered): 8,109,434 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2546 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.1% C: 3.8% G: 90.0% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 466.7 0 1 4 1 116.7 0 1 5 1 29.2 0 1 6 1 7.3 0 1 9 5 0.1 0 1 4 10 408 0.0 1 28 380 11 2062 0.0 1 19 2043 12 9 0.0 1 0 9 14 1 0.0 1 0 1 104 1 0.0 1 1 151 7 0.0 1 7 209 7 0.0 1 5 2 259 35 0.0 1 34 1 260 2 0.0 1 2 261 4 0.0 1 4 262 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.