This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.43 s (25 us/read; 2.39 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 60,674 (2.0%) Reads that were too short: 12,969 (0.4%) Reads written (passing filters): 47,705 (1.6%) Total basepairs processed: 905,907,359 bp Total written (filtered): 11,939,404 bp (1.3%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 60674 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1015 734.8 0 1015 7 396 734.8 0 396 8 29182 734.8 0 29182 9 2983 734.8 0 2983 10 20 734.8 0 20 11 14 734.8 0 14 12 9 734.8 0 9 13 10 734.8 0 10 14 11 734.8 0 11 15 8 734.8 0 8 16 10 734.8 0 10 17 9 734.8 0 9 18 10 734.8 0 10 19 23 734.8 0 23 20 10 734.8 0 10 21 29 734.8 0 29 22 25 734.8 0 25 23 17 734.8 0 17 24 15 734.8 0 15 25 12 734.8 0 12 26 26 734.8 0 26 27 21 734.8 0 21 28 15 734.8 0 15 29 43 734.8 0 43 30 13 734.8 0 13 31 13 734.8 0 13 32 11 734.8 0 11 33 14 734.8 0 14 34 19 734.8 0 19 35 12 734.8 0 12 36 12 734.8 0 12 37 30 734.8 0 30 38 21 734.8 0 21 39 19 734.8 0 19 40 10 734.8 0 10 41 20 734.8 0 20 42 13 734.8 0 13 43 10 734.8 0 10 44 17 734.8 0 17 45 18 734.8 0 18 46 18 734.8 0 18 47 22 734.8 0 22 48 17 734.8 0 17 49 17 734.8 0 17 50 17 734.8 0 17 51 21 734.8 0 21 52 23 734.8 0 23 53 15 734.8 0 15 54 30 734.8 0 30 55 27 734.8 0 27 56 13 734.8 0 13 57 25 734.8 0 25 58 16 734.8 0 16 59 13 734.8 0 13 60 26 734.8 0 26 61 14 734.8 0 14 62 22 734.8 0 22 63 18 734.8 0 18 64 25 734.8 0 25 65 37 734.8 0 37 66 22 734.8 0 22 67 14 734.8 0 14 68 17 734.8 0 17 69 17 734.8 0 17 70 39 734.8 0 39 71 39 734.8 0 39 72 50 734.8 0 50 73 15 734.8 0 15 74 21 734.8 0 21 75 31 734.8 0 31 76 23 734.8 0 23 77 25 734.8 0 25 78 28 734.8 0 28 79 177 734.8 0 177 80 185 734.8 0 185 81 46 734.8 0 46 82 29 734.8 0 29 83 27 734.8 0 27 84 32 734.8 0 32 85 18 734.8 0 18 86 18 734.8 0 18 87 27 734.8 0 27 88 25 734.8 0 25 89 19 734.8 0 19 90 13 734.8 0 13 91 7 734.8 0 7 92 12 734.8 0 12 93 13 734.8 0 13 94 16 734.8 0 16 95 29 734.8 0 29 96 62 734.8 0 62 97 89 734.8 0 89 98 36 734.8 0 36 99 38 734.8 0 38 100 50 734.8 0 50 101 34 734.8 0 34 102 38 734.8 0 38 103 20 734.8 0 20 104 24 734.8 0 24 105 29 734.8 0 29 106 16 734.8 0 16 107 24 734.8 0 24 108 20 734.8 0 20 109 52 734.8 0 52 110 26 734.8 0 26 111 18 734.8 0 18 112 55 734.8 0 55 113 39 734.8 0 39 114 39 734.8 0 39 115 15 734.8 0 15 116 30 734.8 0 30 117 200 734.8 0 200 118 153 734.8 0 153 119 90 734.8 0 90 120 25 734.8 0 25 121 21 734.8 0 21 122 30 734.8 0 30 123 36 734.8 0 36 124 33 734.8 0 33 125 35 734.8 0 35 126 39 734.8 0 39 127 86 734.8 0 86 128 43 734.8 0 43 129 28 734.8 0 28 130 59 734.8 0 59 131 104 734.8 0 104 132 113 734.8 0 113 133 91 734.8 0 91 134 77 734.8 0 77 135 57 734.8 0 57 136 51 734.8 0 51 137 51 734.8 0 51 138 105 734.8 0 105 139 138 734.8 0 138 140 54 734.8 0 54 141 37 734.8 0 37 142 33 734.8 0 33 143 38 734.8 0 38 144 31 734.8 0 31 145 68 734.8 0 68 146 67 734.8 0 67 147 69 734.8 0 69 148 47 734.8 0 47 149 32 734.8 0 32 150 23 734.8 0 23 151 26 734.8 0 26 152 31 734.8 0 31 153 23 734.8 0 23 154 26 734.8 0 26 155 28 734.8 0 28 156 108 734.8 0 108 157 79 734.8 0 79 158 58 734.8 0 58 159 38 734.8 0 38 160 41 734.8 0 41 161 56 734.8 0 56 162 39 734.8 0 39 163 39 734.8 0 39 164 36 734.8 0 36 165 39 734.8 0 39 166 56 734.8 0 56 167 53 734.8 0 53 168 86 734.8 0 86 169 71 734.8 0 71 170 54 734.8 0 54 171 49 734.8 0 49 172 52 734.8 0 52 173 63 734.8 0 63 174 53 734.8 0 53 175 73 734.8 0 73 176 212 734.8 0 212 177 481 734.8 0 481 178 1194 734.8 0 1194 179 746 734.8 0 746 180 1619 734.8 0 1619 181 567 734.8 0 567 182 434 734.8 0 434 183 97 734.8 0 97 184 84 734.8 0 84 185 65 734.8 0 65 186 51 734.8 0 51 187 50 734.8 0 50 188 56 734.8 0 56 189 60 734.8 0 60 190 41 734.8 0 41 191 316 734.8 0 316 192 48 734.8 0 48 193 41 734.8 0 41 194 103 734.8 0 103 195 178 734.8 0 178 196 342 734.8 0 342 197 527 734.8 0 527 198 231 734.8 0 231 199 55 734.8 0 55 200 75 734.8 0 75 201 222 734.8 0 222 202 127 734.8 0 127 203 80 734.8 0 80 204 43 734.8 0 43 205 33 734.8 0 33 206 35 734.8 0 35 207 28 734.8 0 28 208 36 734.8 0 36 209 32 734.8 0 32 210 30 734.8 0 30 211 26 734.8 0 26 212 28 734.8 0 28 213 43 734.8 0 43 214 47 734.8 0 47 215 146 734.8 0 146 216 111 734.8 0 111 217 73 734.8 0 73 218 37 734.8 0 37 219 38 734.8 0 38 220 62 734.8 0 62 221 53 734.8 0 53 222 61 734.8 0 61 223 175 734.8 0 175 224 176 734.8 0 176 225 70 734.8 0 70 226 34 734.8 0 34 227 32 734.8 0 32 228 36 734.8 0 36 229 58 734.8 0 58 230 58 734.8 0 58 231 31 734.8 0 31 232 44 734.8 0 44 233 43 734.8 0 43 234 154 734.8 0 154 235 436 734.8 0 436 236 87 734.8 0 87 237 60 734.8 0 60 238 65 734.8 0 65 239 274 734.8 0 274 240 459 734.8 0 459 241 238 734.8 0 238 242 104 734.8 0 104 243 80 734.8 0 80 244 542 734.8 0 542 245 32 734.8 0 32 246 37 734.8 0 37 247 30 734.8 0 30 248 72 734.8 0 72 249 69 734.8 0 69 250 55 734.8 0 55 251 21 734.8 0 21 252 40 734.8 0 40 253 37 734.8 0 37 254 67 734.8 0 67 255 63 734.8 0 63 256 65 734.8 0 65 257 142 734.8 0 142 258 156 734.8 0 156 259 784 734.8 0 784 260 963 734.8 0 963 261 293 734.8 0 293 262 50 734.8 0 50 263 108 734.8 0 108 264 344 734.8 0 344 265 553 734.8 0 553 266 317 734.8 0 317 267 71 734.8 0 71 268 65 734.8 0 65 269 52 734.8 0 52 270 26 734.8 0 26 271 18 734.8 0 18 272 23 734.8 0 23 273 28 734.8 0 28 274 39 734.8 0 39 275 78 734.8 0 78 276 52 734.8 0 52 277 36 734.8 0 36 278 38 734.8 0 38 279 22 734.8 0 22 280 44 734.8 0 44 281 46 734.8 0 46 282 30 734.8 0 30 283 24 734.8 0 24 284 34 734.8 0 34 285 35 734.8 0 35 286 34 734.8 0 34 287 77 734.8 0 77 288 86 734.8 0 86 289 63 734.8 0 63 290 79 734.8 0 79 291 854 734.8 0 854 292 835 734.8 0 835 293 637 734.8 0 637 294 300 734.8 0 300 295 108 734.8 0 108 296 95 734.8 0 95 297 97 734.8 0 97 298 40 734.8 0 40 299 42 734.8 0 42 300 115 734.8 0 115 301 23 734.8 0 23 Finished in 2.08 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 47,705 Reads with adapters: 30,421 (63.8%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 30,401 (63.7%) Total basepairs processed: 11,939,404 bp Total written (filtered): 8,387,699 bp (70.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30421 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 6 0.0 3 0 0 6 16 249 0.0 3 0 246 1 2 17 29167 0.0 3 28689 474 4 18 78 0.0 3 7 63 7 1 19 24 0.0 3 0 8 14 2 20 849 0.0 3 807 11 0 31 21 3 0.0 3 0 3 23 3 0.0 3 0 0 0 3 25 2 0.0 3 2 32 1 0.0 3 1 35 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 60 3 0.0 3 0 0 0 3 63 4 0.0 3 0 0 0 4 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 98 2 0.0 3 0 0 1 1 99 1 0.0 3 0 0 0 1 102 3 0.0 3 0 0 0 3 105 3 0.0 3 0 0 0 3 106 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.10 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 30,401 Reads with adapters: 2,122 (7.0%) Reads written (passing filters): 30,401 (100.0%) Total basepairs processed: 8,387,699 bp Total written (filtered): 8,381,331 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2122 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 74.2% C: 2.6% G: 22.5% T: 0.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2120 475.0 0 2120 4 2 118.8 0 2