This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.91 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 235,578 (7.8%) Reads that were too short: 120,628 (4.0%) Reads written (passing filters): 114,950 (3.8%) Total basepairs processed: 905,907,359 bp Total written (filtered): 22,945,001 bp (2.5%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 235578 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 484 734.8 0 484 7 25174 734.8 0 25174 8 67 734.8 0 67 9 2270 734.8 0 2270 10 37 734.8 0 37 11 15 734.8 0 15 12 15 734.8 0 15 13 49 734.8 0 49 14 16 734.8 0 16 15 9 734.8 0 9 16 9 734.8 0 9 17 14 734.8 0 14 18 16 734.8 0 16 19 6 734.8 0 6 20 10 734.8 0 10 21 7 734.8 0 7 22 6 734.8 0 6 23 8 734.8 0 8 24 9 734.8 0 9 25 19 734.8 0 19 26 11 734.8 0 11 27 11 734.8 0 11 28 9 734.8 0 9 29 9 734.8 0 9 30 6 734.8 0 6 31 21 734.8 0 21 32 14 734.8 0 14 33 13 734.8 0 13 34 14 734.8 0 14 35 17 734.8 0 17 36 6 734.8 0 6 37 12 734.8 0 12 38 15 734.8 0 15 39 14 734.8 0 14 40 8 734.8 0 8 41 13 734.8 0 13 42 14 734.8 0 14 43 10 734.8 0 10 44 12 734.8 0 12 45 7 734.8 0 7 46 9 734.8 0 9 47 11 734.8 0 11 48 13 734.8 0 13 49 10 734.8 0 10 50 12 734.8 0 12 51 10 734.8 0 10 52 9 734.8 0 9 53 11 734.8 0 11 54 63 734.8 0 63 55 8648 734.8 0 8648 56 107 734.8 0 107 57 14 734.8 0 14 58 11 734.8 0 11 59 7 734.8 0 7 60 11 734.8 0 11 61 32 734.8 0 32 62 22 734.8 0 22 63 13 734.8 0 13 64 42 734.8 0 42 65 434 734.8 0 434 66 296 734.8 0 296 67 274 734.8 0 274 68 9 734.8 0 9 69 15 734.8 0 15 70 30 734.8 0 30 71 32 734.8 0 32 72 36 734.8 0 36 73 55 734.8 0 55 74 89 734.8 0 89 75 106 734.8 0 106 76 448 734.8 0 448 77 238 734.8 0 238 78 194 734.8 0 194 79 175 734.8 0 175 80 331 734.8 0 331 81 664 734.8 0 664 82 602 734.8 0 602 83 353 734.8 0 353 84 353 734.8 0 353 85 353 734.8 0 353 86 663 734.8 0 663 87 449 734.8 0 449 88 361 734.8 0 361 89 658 734.8 0 658 90 329 734.8 0 329 91 274 734.8 0 274 92 321 734.8 0 321 93 427 734.8 0 427 94 352 734.8 0 352 95 515 734.8 0 515 96 254 734.8 0 254 97 498 734.8 0 498 98 410 734.8 0 410 99 287 734.8 0 287 100 371 734.8 0 371 101 446 734.8 0 446 102 957 734.8 0 957 103 596 734.8 0 596 104 470 734.8 0 470 105 445 734.8 0 445 106 560 734.8 0 560 107 678 734.8 0 678 108 582 734.8 0 582 109 707 734.8 0 707 110 823 734.8 0 823 111 695 734.8 0 695 112 535 734.8 0 535 113 706 734.8 0 706 114 481 734.8 0 481 115 451 734.8 0 451 116 330 734.8 0 330 117 422 734.8 0 422 118 812 734.8 0 812 119 873 734.8 0 873 120 529 734.8 0 529 121 349 734.8 0 349 122 501 734.8 0 501 123 690 734.8 0 690 124 657 734.8 0 657 125 864 734.8 0 864 126 870 734.8 0 870 127 809 734.8 0 809 128 532 734.8 0 532 129 416 734.8 0 416 130 441 734.8 0 441 131 522 734.8 0 522 132 699 734.8 0 699 133 1223 734.8 0 1223 134 990 734.8 0 990 135 539 734.8 0 539 136 509 734.8 0 509 137 585 734.8 0 585 138 902 734.8 0 902 139 1176 734.8 0 1176 140 872 734.8 0 872 141 487 734.8 0 487 142 398 734.8 0 398 143 566 734.8 0 566 144 1009 734.8 0 1009 145 883 734.8 0 883 146 916 734.8 0 916 147 757 734.8 0 757 148 529 734.8 0 529 149 726 734.8 0 726 150 625 734.8 0 625 151 912 734.8 0 912 152 974 734.8 0 974 153 736 734.8 0 736 154 821 734.8 0 821 155 562 734.8 0 562 156 755 734.8 0 755 157 1104 734.8 0 1104 158 634 734.8 0 634 159 848 734.8 0 848 160 662 734.8 0 662 161 623 734.8 0 623 162 532 734.8 0 532 163 510 734.8 0 510 164 879 734.8 0 879 165 768 734.8 0 768 166 588 734.8 0 588 167 462 734.8 0 462 168 493 734.8 0 493 169 467 734.8 0 467 170 466 734.8 0 466 171 520 734.8 0 520 172 833 734.8 0 833 173 674 734.8 0 674 174 537 734.8 0 537 175 332 734.8 0 332 176 479 734.8 0 479 177 487 734.8 0 487 178 453 734.8 0 453 179 740 734.8 0 740 180 844 734.8 0 844 181 881 734.8 0 881 182 542 734.8 0 542 183 476 734.8 0 476 184 686 734.8 0 686 185 754 734.8 0 754 186 628 734.8 0 628 187 489 734.8 0 489 188 612 734.8 0 612 189 760 734.8 0 760 190 778 734.8 0 778 191 604 734.8 0 604 192 496 734.8 0 496 193 481 734.8 0 481 194 552 734.8 0 552 195 451 734.8 0 451 196 684 734.8 0 684 197 764 734.8 0 764 198 398 734.8 0 398 199 810 734.8 0 810 200 658 734.8 0 658 201 824 734.8 0 824 202 863 734.8 0 863 203 530 734.8 0 530 204 534 734.8 0 534 205 723 734.8 0 723 206 988 734.8 0 988 207 861 734.8 0 861 208 788 734.8 0 788 209 881 734.8 0 881 210 1160 734.8 0 1160 211 5078 734.8 0 5078 212 19899 734.8 0 19899 213 4049 734.8 0 4049 214 1513 734.8 0 1513 215 693 734.8 0 693 216 551 734.8 0 551 217 480 734.8 0 480 218 700 734.8 0 700 219 872 734.8 0 872 220 916 734.8 0 916 221 551 734.8 0 551 222 664 734.8 0 664 223 692 734.8 0 692 224 817 734.8 0 817 225 791 734.8 0 791 226 692 734.8 0 692 227 525 734.8 0 525 228 525 734.8 0 525 229 629 734.8 0 629 230 640 734.8 0 640 231 612 734.8 0 612 232 607 734.8 0 607 233 665 734.8 0 665 234 760 734.8 0 760 235 597 734.8 0 597 236 695 734.8 0 695 237 549 734.8 0 549 238 1040 734.8 0 1040 239 872 734.8 0 872 240 899 734.8 0 899 241 978 734.8 0 978 242 819 734.8 0 819 243 661 734.8 0 661 244 531 734.8 0 531 245 772 734.8 0 772 246 620 734.8 0 620 247 579 734.8 0 579 248 441 734.8 0 441 249 743 734.8 0 743 250 801 734.8 0 801 251 1030 734.8 0 1030 252 976 734.8 0 976 253 529 734.8 0 529 254 561 734.8 0 561 255 642 734.8 0 642 256 657 734.8 0 657 257 640 734.8 0 640 258 604 734.8 0 604 259 760 734.8 0 760 260 923 734.8 0 923 261 1048 734.8 0 1048 262 974 734.8 0 974 263 700 734.8 0 700 264 581 734.8 0 581 265 704 734.8 0 704 266 716 734.8 0 716 267 835 734.8 0 835 268 660 734.8 0 660 269 663 734.8 0 663 270 1469 734.8 0 1469 271 2800 734.8 0 2800 272 1199 734.8 0 1199 273 836 734.8 0 836 274 1871 734.8 0 1871 275 1044 734.8 0 1044 276 1072 734.8 0 1072 277 1312 734.8 0 1312 278 1324 734.8 0 1324 279 812 734.8 0 812 280 660 734.8 0 660 281 573 734.8 0 573 282 846 734.8 0 846 283 1039 734.8 0 1039 284 749 734.8 0 749 285 584 734.8 0 584 286 801 734.8 0 801 287 737 734.8 0 737 288 1017 734.8 0 1017 289 1148 734.8 0 1148 290 708 734.8 0 708 291 930 734.8 0 930 292 1980 734.8 0 1980 293 4630 734.8 0 4630 294 4013 734.8 0 4013 295 1433 734.8 0 1433 296 888 734.8 0 888 297 836 734.8 0 836 298 904 734.8 0 904 299 991 734.8 0 991 300 1517 734.8 0 1517 301 2856 734.8 0 2856 Finished in 4.64 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 114,950 Reads with adapters: 26,636 (23.2%) Reads that were too short: 39 (0.0%) Reads written (passing filters): 26,597 (23.1%) Total basepairs processed: 22,945,001 bp Total written (filtered): 7,188,855 bp (31.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 26636 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 16 0.0 3 0 0 10 2 4 19 229 0.0 3 0 217 4 5 3 20 25202 0.0 4 24684 441 47 18 12 21 56 0.0 4 8 46 2 22 6 0.0 4 0 1 4 0 1 23 20 0.0 4 1 0 0 16 3 24 1 0.0 4 1 27 2 0.0 4 0 0 0 1 1 29 1 0.0 4 0 0 0 0 1 30 2 0.0 4 0 0 0 1 1 32 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 34 1 0.0 4 1 42 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 45 2 0.0 4 0 0 0 0 2 46 2 0.0 4 0 0 0 0 2 51 2 0.0 4 0 0 0 0 2 52 1 0.0 4 0 0 0 0 1 53 964 0.0 4 0 0 1 864 99 54 3 0.0 4 0 0 0 1 2 59 1 0.0 4 0 0 0 0 1 60 3 0.0 4 1 0 0 0 2 62 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 75 2 0.0 4 0 0 0 0 2 79 2 0.0 4 0 0 0 0 2 81 2 0.0 4 0 0 0 0 2 84 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 102 3 0.0 4 0 0 0 0 3 103 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 107 4 0.0 4 0 0 0 0 4 108 1 0.0 4 0 0 0 0 1 109 18 0.0 4 0 0 0 18 110 2 0.0 4 0 0 0 1 1 111 17 0.0 4 0 0 0 15 2 124 2 0.0 4 0 0 0 0 2 127 35 0.0 4 0 0 0 0 35 132 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 141 2 0.0 4 0 0 0 0 2 142 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 157 2 0.0 4 0 0 0 0 2 167 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.92 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 26,597 Reads with adapters: 3,390 (12.7%) Reads written (passing filters): 26,597 (100.0%) Total basepairs processed: 7,188,855 bp Total written (filtered): 7,117,636 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3390 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.2% C: 3.3% G: 90.1% T: 2.4% none/other: 0.1% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 415.6 0 3 4 1 103.9 0 1 10 3 0.0 1 1 2 11 3206 0.0 1 23 3183 12 43 0.0 1 1 42 123 1 0.0 1 0 1 210 1 0.0 1 1 234 2 0.0 1 2 259 2 0.0 1 2 261 60 0.0 1 57 3 262 1 0.0 1 1 269 1 0.0 1 1 270 2 0.0 1 2 271 64 0.0 1 62 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.