This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.57 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 213,082 (7.1%) Reads that were too short: 130,128 (4.3%) Reads written (passing filters): 82,954 (2.8%) Total basepairs processed: 905,907,359 bp Total written (filtered): 19,225,359 bp (2.1%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 213082 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 719 734.8 0 719 7 537 734.8 0 537 8 39438 734.8 0 39438 9 4679 734.8 0 4679 10 44 734.8 0 44 11 56 734.8 0 56 12 35 734.8 0 35 13 53 734.8 0 53 14 47 734.8 0 47 15 26 734.8 0 26 16 40 734.8 0 40 17 33 734.8 0 33 18 90 734.8 0 90 19 34 734.8 0 34 20 37 734.8 0 37 21 33 734.8 0 33 22 31 734.8 0 31 23 29 734.8 0 29 24 39 734.8 0 39 25 26 734.8 0 26 26 30 734.8 0 30 27 35 734.8 0 35 28 37 734.8 0 37 29 17 734.8 0 17 30 28 734.8 0 28 31 36 734.8 0 36 32 37 734.8 0 37 33 25 734.8 0 25 34 29 734.8 0 29 35 29 734.8 0 29 36 38 734.8 0 38 37 91 734.8 0 91 38 106 734.8 0 106 39 56 734.8 0 56 40 29 734.8 0 29 41 156 734.8 0 156 42 52 734.8 0 52 43 39 734.8 0 39 44 131 734.8 0 131 45 42 734.8 0 42 46 76 734.8 0 76 47 99 734.8 0 99 48 103 734.8 0 103 49 48 734.8 0 48 50 37 734.8 0 37 51 44 734.8 0 44 52 82 734.8 0 82 53 215 734.8 0 215 54 233 734.8 0 233 55 75 734.8 0 75 56 46 734.8 0 46 57 444 734.8 0 444 58 57 734.8 0 57 59 28 734.8 0 28 60 27 734.8 0 27 61 36 734.8 0 36 62 31 734.8 0 31 63 24 734.8 0 24 64 36 734.8 0 36 65 47 734.8 0 47 66 59 734.8 0 59 67 35 734.8 0 35 68 40 734.8 0 40 69 31 734.8 0 31 70 28 734.8 0 28 71 27 734.8 0 27 72 57 734.8 0 57 73 291 734.8 0 291 74 152 734.8 0 152 75 149 734.8 0 149 76 72 734.8 0 72 77 82 734.8 0 82 78 89 734.8 0 89 79 76 734.8 0 76 80 43 734.8 0 43 81 128 734.8 0 128 82 108 734.8 0 108 83 98 734.8 0 98 84 107 734.8 0 107 85 156 734.8 0 156 86 144 734.8 0 144 87 100 734.8 0 100 88 115 734.8 0 115 89 108 734.8 0 108 90 83 734.8 0 83 91 145 734.8 0 145 92 103 734.8 0 103 93 168 734.8 0 168 94 245 734.8 0 245 95 149 734.8 0 149 96 148 734.8 0 148 97 177 734.8 0 177 98 236 734.8 0 236 99 135 734.8 0 135 100 55 734.8 0 55 101 45 734.8 0 45 102 48 734.8 0 48 103 76 734.8 0 76 104 43 734.8 0 43 105 35 734.8 0 35 106 75 734.8 0 75 107 115 734.8 0 115 108 94 734.8 0 94 109 82 734.8 0 82 110 95 734.8 0 95 111 83 734.8 0 83 112 86 734.8 0 86 113 82 734.8 0 82 114 129 734.8 0 129 115 195 734.8 0 195 116 184 734.8 0 184 117 125 734.8 0 125 118 123 734.8 0 123 119 203 734.8 0 203 120 263 734.8 0 263 121 157 734.8 0 157 122 101 734.8 0 101 123 117 734.8 0 117 124 136 734.8 0 136 125 187 734.8 0 187 126 214 734.8 0 214 127 250 734.8 0 250 128 253 734.8 0 253 129 197 734.8 0 197 130 172 734.8 0 172 131 145 734.8 0 145 132 123 734.8 0 123 133 161 734.8 0 161 134 291 734.8 0 291 135 346 734.8 0 346 136 326 734.8 0 326 137 288 734.8 0 288 138 174 734.8 0 174 139 134 734.8 0 134 140 189 734.8 0 189 141 211 734.8 0 211 142 136 734.8 0 136 143 133 734.8 0 133 144 202 734.8 0 202 145 1237 734.8 0 1237 146 672 734.8 0 672 147 472 734.8 0 472 148 224 734.8 0 224 149 159 734.8 0 159 150 170 734.8 0 170 151 187 734.8 0 187 152 242 734.8 0 242 153 281 734.8 0 281 154 352 734.8 0 352 155 317 734.8 0 317 156 298 734.8 0 298 157 351 734.8 0 351 158 282 734.8 0 282 159 251 734.8 0 251 160 251 734.8 0 251 161 263 734.8 0 263 162 241 734.8 0 241 163 195 734.8 0 195 164 256 734.8 0 256 165 530 734.8 0 530 166 485 734.8 0 485 167 296 734.8 0 296 168 199 734.8 0 199 169 203 734.8 0 203 170 211 734.8 0 211 171 214 734.8 0 214 172 237 734.8 0 237 173 1102 734.8 0 1102 174 2303 734.8 0 2303 175 1451 734.8 0 1451 176 448 734.8 0 448 177 185 734.8 0 185 178 211 734.8 0 211 179 281 734.8 0 281 180 312 734.8 0 312 181 577 734.8 0 577 182 733 734.8 0 733 183 529 734.8 0 529 184 296 734.8 0 296 185 354 734.8 0 354 186 627 734.8 0 627 187 512 734.8 0 512 188 347 734.8 0 347 189 268 734.8 0 268 190 250 734.8 0 250 191 350 734.8 0 350 192 496 734.8 0 496 193 702 734.8 0 702 194 342 734.8 0 342 195 191 734.8 0 191 196 164 734.8 0 164 197 228 734.8 0 228 198 309 734.8 0 309 199 279 734.8 0 279 200 165 734.8 0 165 201 246 734.8 0 246 202 310 734.8 0 310 203 315 734.8 0 315 204 347 734.8 0 347 205 327 734.8 0 327 206 509 734.8 0 509 207 462 734.8 0 462 208 295 734.8 0 295 209 219 734.8 0 219 210 218 734.8 0 218 211 238 734.8 0 238 212 299 734.8 0 299 213 316 734.8 0 316 214 348 734.8 0 348 215 347 734.8 0 347 216 327 734.8 0 327 217 351 734.8 0 351 218 408 734.8 0 408 219 549 734.8 0 549 220 463 734.8 0 463 221 474 734.8 0 474 222 453 734.8 0 453 223 454 734.8 0 454 224 418 734.8 0 418 225 4063 734.8 0 4063 226 9173 734.8 0 9173 227 1699 734.8 0 1699 228 318 734.8 0 318 229 442 734.8 0 442 230 2504 734.8 0 2504 231 4235 734.8 0 4235 232 3304 734.8 0 3304 233 2154 734.8 0 2154 234 1031 734.8 0 1031 235 707 734.8 0 707 236 297 734.8 0 297 237 256 734.8 0 256 238 320 734.8 0 320 239 384 734.8 0 384 240 222 734.8 0 222 241 322 734.8 0 322 242 394 734.8 0 394 243 338 734.8 0 338 244 332 734.8 0 332 245 325 734.8 0 325 246 707 734.8 0 707 247 633 734.8 0 633 248 396 734.8 0 396 249 184 734.8 0 184 250 9076 734.8 0 9076 251 10982 734.8 0 10982 252 4825 734.8 0 4825 253 2183 734.8 0 2183 254 1366 734.8 0 1366 255 551 734.8 0 551 256 447 734.8 0 447 257 346 734.8 0 346 258 222 734.8 0 222 259 131 734.8 0 131 260 172 734.8 0 172 261 187 734.8 0 187 262 278 734.8 0 278 263 246 734.8 0 246 264 253 734.8 0 253 265 570 734.8 0 570 266 549 734.8 0 549 267 503 734.8 0 503 268 344 734.8 0 344 269 252 734.8 0 252 270 388 734.8 0 388 271 902 734.8 0 902 272 2259 734.8 0 2259 273 434 734.8 0 434 274 369 734.8 0 369 275 448 734.8 0 448 276 418 734.8 0 418 277 434 734.8 0 434 278 639 734.8 0 639 279 859 734.8 0 859 280 2456 734.8 0 2456 281 2370 734.8 0 2370 282 1378 734.8 0 1378 283 457 734.8 0 457 284 559 734.8 0 559 285 1225 734.8 0 1225 286 749 734.8 0 749 287 417 734.8 0 417 288 409 734.8 0 409 289 455 734.8 0 455 290 880 734.8 0 880 291 883 734.8 0 883 292 763 734.8 0 763 293 473 734.8 0 473 294 702 734.8 0 702 295 809 734.8 0 809 296 1010 734.8 0 1010 297 1233 734.8 0 1233 298 1994 734.8 0 1994 299 3150 734.8 0 3150 300 4359 734.8 0 4359 301 19577 734.8 0 19577 Finished in 3.76 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 82,954 Reads with adapters: 5,261 (6.3%) Reads that were too short: 47 (0.1%) Reads written (passing filters): 5,214 (6.3%) Total basepairs processed: 19,225,359 bp Total written (filtered): 1,361,662 bp (7.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5261 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 52 0.0 3 0 42 3 3 4 20 4595 0.0 4 4278 281 23 6 7 21 13 0.0 4 3 8 1 1 23 9 0.0 4 0 0 0 8 1 25 2 0.0 4 0 0 0 0 2 34 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 41 1 0.0 4 1 42 1 0.0 4 1 44 1 0.0 4 0 0 0 1 51 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 1 86 1 0.0 4 0 0 0 0 1 87 2 0.0 4 0 0 0 0 2 89 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 103 2 0.0 4 0 0 0 0 2 108 4 0.0 4 0 0 0 3 1 109 12 0.0 4 0 0 0 12 110 1 0.0 4 0 0 0 1 111 23 0.0 4 0 0 0 22 1 123 1 0.0 4 0 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 7 0.0 4 0 0 0 0 7 127 478 0.0 4 0 0 0 0 478 129 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 1 160 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 228 8 0.0 4 0 0 0 0 8 235 1 0.0 4 0 0 0 0 1 239 2 0.0 4 0 0 0 0 2 240 4 0.0 4 0 0 0 0 4 241 3 0.0 4 0 0 0 0 3 243 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 282 4 0.0 4 0 0 0 0 4 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.18 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 5,214 Reads with adapters: 248 (4.8%) Reads written (passing filters): 5,214 (100.0%) Total basepairs processed: 1,361,662 bp Total written (filtered): 1,356,854 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 248 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 6.9% C: 5.6% G: 85.9% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 50 0.0 0 5 45 10 129 0.0 1 6 123 11 57 0.0 1 0 57 12 1 0.0 1 0 1 208 4 0.0 1 2 2 223 5 0.0 1 5 224 1 0.0 1 1 258 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.