This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.66 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 343,579 (11.4%) Reads that were too short: 120,139 (4.0%) Reads written (passing filters): 223,440 (7.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 42,686,655 bp (4.7%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 343579 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 437 734.8 0 437 7 46027 734.8 0 46027 8 100 734.8 0 100 9 4361 734.8 0 4361 10 25 734.8 0 25 11 42 734.8 0 42 12 37 734.8 0 37 13 19 734.8 0 19 14 17 734.8 0 17 15 34 734.8 0 34 16 20 734.8 0 20 17 10 734.8 0 10 18 19 734.8 0 19 19 14 734.8 0 14 20 20 734.8 0 20 21 7 734.8 0 7 22 12 734.8 0 12 23 11 734.8 0 11 24 13 734.8 0 13 25 12 734.8 0 12 26 18 734.8 0 18 27 22 734.8 0 22 28 26 734.8 0 26 29 20 734.8 0 20 30 14 734.8 0 14 31 18 734.8 0 18 32 25 734.8 0 25 33 17 734.8 0 17 34 11 734.8 0 11 35 25 734.8 0 25 36 25 734.8 0 25 37 15 734.8 0 15 38 46 734.8 0 46 39 77 734.8 0 77 40 265 734.8 0 265 41 25 734.8 0 25 42 15 734.8 0 15 43 24 734.8 0 24 44 17 734.8 0 17 45 13 734.8 0 13 46 10 734.8 0 10 47 21 734.8 0 21 48 9 734.8 0 9 49 26 734.8 0 26 50 20 734.8 0 20 51 20 734.8 0 20 52 14 734.8 0 14 53 22 734.8 0 22 54 104 734.8 0 104 55 19 734.8 0 19 56 21 734.8 0 21 57 13 734.8 0 13 58 57 734.8 0 57 59 55 734.8 0 55 60 23 734.8 0 23 61 24 734.8 0 24 62 28 734.8 0 28 63 251 734.8 0 251 64 13 734.8 0 13 65 19 734.8 0 19 66 13 734.8 0 13 67 15 734.8 0 15 68 19 734.8 0 19 69 261 734.8 0 261 70 32 734.8 0 32 71 28 734.8 0 28 72 27 734.8 0 27 73 194 734.8 0 194 74 74 734.8 0 74 75 54 734.8 0 54 76 44 734.8 0 44 77 149 734.8 0 149 78 434 734.8 0 434 79 747 734.8 0 747 80 1820 734.8 0 1820 81 1436 734.8 0 1436 82 1651 734.8 0 1651 83 2142 734.8 0 2142 84 1841 734.8 0 1841 85 1474 734.8 0 1474 86 2060 734.8 0 2060 87 2301 734.8 0 2301 88 988 734.8 0 988 89 536 734.8 0 536 90 583 734.8 0 583 91 1005 734.8 0 1005 92 2275 734.8 0 2275 93 906 734.8 0 906 94 1327 734.8 0 1327 95 1093 734.8 0 1093 96 745 734.8 0 745 97 639 734.8 0 639 98 554 734.8 0 554 99 508 734.8 0 508 100 1649 734.8 0 1649 101 2076 734.8 0 2076 102 1261 734.8 0 1261 103 1142 734.8 0 1142 104 775 734.8 0 775 105 501 734.8 0 501 106 398 734.8 0 398 107 412 734.8 0 412 108 1035 734.8 0 1035 109 1340 734.8 0 1340 110 1482 734.8 0 1482 111 1127 734.8 0 1127 112 1409 734.8 0 1409 113 1065 734.8 0 1065 114 819 734.8 0 819 115 1064 734.8 0 1064 116 1575 734.8 0 1575 117 1803 734.8 0 1803 118 1928 734.8 0 1928 119 1722 734.8 0 1722 120 1869 734.8 0 1869 121 1791 734.8 0 1791 122 1709 734.8 0 1709 123 1369 734.8 0 1369 124 1316 734.8 0 1316 125 1412 734.8 0 1412 126 1235 734.8 0 1235 127 991 734.8 0 991 128 1098 734.8 0 1098 129 1254 734.8 0 1254 130 1272 734.8 0 1272 131 1988 734.8 0 1988 132 1349 734.8 0 1349 133 1076 734.8 0 1076 134 1107 734.8 0 1107 135 1086 734.8 0 1086 136 1312 734.8 0 1312 137 1380 734.8 0 1380 138 1638 734.8 0 1638 139 1589 734.8 0 1589 140 1175 734.8 0 1175 141 1187 734.8 0 1187 142 1616 734.8 0 1616 143 1346 734.8 0 1346 144 1136 734.8 0 1136 145 1573 734.8 0 1573 146 1589 734.8 0 1589 147 1545 734.8 0 1545 148 1470 734.8 0 1470 149 1200 734.8 0 1200 150 1371 734.8 0 1371 151 1298 734.8 0 1298 152 1483 734.8 0 1483 153 1555 734.8 0 1555 154 1247 734.8 0 1247 155 1420 734.8 0 1420 156 1161 734.8 0 1161 157 1313 734.8 0 1313 158 1473 734.8 0 1473 159 1379 734.8 0 1379 160 1437 734.8 0 1437 161 1139 734.8 0 1139 162 1288 734.8 0 1288 163 1066 734.8 0 1066 164 1097 734.8 0 1097 165 859 734.8 0 859 166 2002 734.8 0 2002 167 4028 734.8 0 4028 168 1651 734.8 0 1651 169 1313 734.8 0 1313 170 2349 734.8 0 2349 171 2460 734.8 0 2460 172 1735 734.8 0 1735 173 1121 734.8 0 1121 174 1093 734.8 0 1093 175 1367 734.8 0 1367 176 1271 734.8 0 1271 177 1413 734.8 0 1413 178 1378 734.8 0 1378 179 1175 734.8 0 1175 180 1529 734.8 0 1529 181 2128 734.8 0 2128 182 2220 734.8 0 2220 183 2243 734.8 0 2243 184 1659 734.8 0 1659 185 1354 734.8 0 1354 186 1179 734.8 0 1179 187 1333 734.8 0 1333 188 1417 734.8 0 1417 189 1262 734.8 0 1262 190 1118 734.8 0 1118 191 1132 734.8 0 1132 192 1191 734.8 0 1191 193 1090 734.8 0 1090 194 1017 734.8 0 1017 195 1209 734.8 0 1209 196 1000 734.8 0 1000 197 1499 734.8 0 1499 198 1746 734.8 0 1746 199 1486 734.8 0 1486 200 1777 734.8 0 1777 201 949 734.8 0 949 202 1088 734.8 0 1088 203 1229 734.8 0 1229 204 1314 734.8 0 1314 205 1323 734.8 0 1323 206 1214 734.8 0 1214 207 1194 734.8 0 1194 208 1025 734.8 0 1025 209 941 734.8 0 941 210 1022 734.8 0 1022 211 1173 734.8 0 1173 212 1137 734.8 0 1137 213 2006 734.8 0 2006 214 2197 734.8 0 2197 215 1374 734.8 0 1374 216 1016 734.8 0 1016 217 1129 734.8 0 1129 218 1447 734.8 0 1447 219 1537 734.8 0 1537 220 1213 734.8 0 1213 221 903 734.8 0 903 222 1034 734.8 0 1034 223 1296 734.8 0 1296 224 1214 734.8 0 1214 225 933 734.8 0 933 226 947 734.8 0 947 227 938 734.8 0 938 228 1153 734.8 0 1153 229 1057 734.8 0 1057 230 934 734.8 0 934 231 904 734.8 0 904 232 976 734.8 0 976 233 1078 734.8 0 1078 234 969 734.8 0 969 235 954 734.8 0 954 236 834 734.8 0 834 237 969 734.8 0 969 238 1261 734.8 0 1261 239 1215 734.8 0 1215 240 1061 734.8 0 1061 241 963 734.8 0 963 242 1067 734.8 0 1067 243 1056 734.8 0 1056 244 997 734.8 0 997 245 937 734.8 0 937 246 1195 734.8 0 1195 247 1063 734.8 0 1063 248 1226 734.8 0 1226 249 1405 734.8 0 1405 250 1213 734.8 0 1213 251 1061 734.8 0 1061 252 1465 734.8 0 1465 253 1091 734.8 0 1091 254 981 734.8 0 981 255 883 734.8 0 883 256 918 734.8 0 918 257 821 734.8 0 821 258 786 734.8 0 786 259 707 734.8 0 707 260 754 734.8 0 754 261 918 734.8 0 918 262 1492 734.8 0 1492 263 1520 734.8 0 1520 264 1098 734.8 0 1098 265 848 734.8 0 848 266 928 734.8 0 928 267 2728 734.8 0 2728 268 6057 734.8 0 6057 269 2984 734.8 0 2984 270 1172 734.8 0 1172 271 877 734.8 0 877 272 1312 734.8 0 1312 273 1188 734.8 0 1188 274 886 734.8 0 886 275 878 734.8 0 878 276 761 734.8 0 761 277 901 734.8 0 901 278 1045 734.8 0 1045 279 1065 734.8 0 1065 280 1295 734.8 0 1295 281 1689 734.8 0 1689 282 870 734.8 0 870 283 928 734.8 0 928 284 879 734.8 0 879 285 718 734.8 0 718 286 920 734.8 0 920 287 894 734.8 0 894 288 776 734.8 0 776 289 690 734.8 0 690 290 1118 734.8 0 1118 291 1327 734.8 0 1327 292 1532 734.8 0 1532 293 1093 734.8 0 1093 294 893 734.8 0 893 295 822 734.8 0 822 296 950 734.8 0 950 297 1486 734.8 0 1486 298 1669 734.8 0 1669 299 1912 734.8 0 1912 300 1718 734.8 0 1718 301 1504 734.8 0 1504 Finished in 7.42 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 223,440 Reads with adapters: 4,551 (2.0%) Reads that were too short: 42 (0.0%) Reads written (passing filters): 4,509 (2.0%) Total basepairs processed: 42,686,655 bp Total written (filtered): 1,215,993 bp (2.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4551 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 1 1 16 37 0.0 3 0 36 0 1 17 4294 0.0 3 4220 73 1 18 7 0.0 3 0 6 1 19 5 0.0 3 0 0 2 3 20 13 0.0 3 0 0 0 13 21 17 0.0 3 0 0 0 17 24 14 0.0 3 0 0 0 14 27 2 0.0 3 0 0 1 1 32 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 52 4 0.0 3 0 0 0 4 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 1 1 64 1 0.0 3 0 0 0 1 66 69 0.0 3 0 0 0 69 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 74 4 0.0 3 0 0 0 4 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 89 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 3 0.0 3 0 0 0 3 102 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 1 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 195 1 0.0 3 0 0 0 1 200 2 0.0 3 0 0 0 2 205 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 1 221 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 283 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 4,509 Reads with adapters: 178 (3.9%) Reads written (passing filters): 4,509 (100.0%) Total basepairs processed: 1,215,993 bp Total written (filtered): 1,215,458 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 178 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 20.8% G: 29.2% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 177 70.5 0 177 4 1 17.6 0 1