This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 79.40 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 102,870 (3.4%) Reads that were too short: 35,319 (1.2%) Reads written (passing filters): 67,551 (2.2%) Total basepairs processed: 905,907,359 bp Total written (filtered): 18,046,665 bp (2.0%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 102870 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 823 734.8 0 823 7 48833 734.8 0 48833 8 2953 734.8 0 2953 9 28 734.8 0 28 10 11 734.8 0 11 11 33 734.8 0 33 12 21 734.8 0 21 13 25 734.8 0 25 14 23 734.8 0 23 15 28 734.8 0 28 16 26 734.8 0 26 17 34 734.8 0 34 18 19 734.8 0 19 19 37 734.8 0 37 20 41 734.8 0 41 21 46 734.8 0 46 22 43 734.8 0 43 23 90 734.8 0 90 24 30 734.8 0 30 25 27 734.8 0 27 26 33 734.8 0 33 27 22 734.8 0 22 28 15 734.8 0 15 29 30 734.8 0 30 30 29 734.8 0 29 31 47 734.8 0 47 32 34 734.8 0 34 33 29 734.8 0 29 34 32 734.8 0 32 35 32 734.8 0 32 36 22 734.8 0 22 37 9 734.8 0 9 38 31 734.8 0 31 39 197 734.8 0 197 40 67 734.8 0 67 41 50 734.8 0 50 42 29 734.8 0 29 43 47 734.8 0 47 44 26 734.8 0 26 45 23 734.8 0 23 46 29 734.8 0 29 47 24 734.8 0 24 48 20 734.8 0 20 49 21 734.8 0 21 50 34 734.8 0 34 51 23 734.8 0 23 52 36 734.8 0 36 53 40 734.8 0 40 54 30 734.8 0 30 55 27 734.8 0 27 56 27 734.8 0 27 57 24 734.8 0 24 58 39 734.8 0 39 59 21 734.8 0 21 60 47 734.8 0 47 61 42 734.8 0 42 62 27 734.8 0 27 63 13 734.8 0 13 64 22 734.8 0 22 65 26 734.8 0 26 66 30 734.8 0 30 67 26 734.8 0 26 68 23 734.8 0 23 69 20 734.8 0 20 70 45 734.8 0 45 71 60 734.8 0 60 72 36 734.8 0 36 73 32 734.8 0 32 74 28 734.8 0 28 75 28 734.8 0 28 76 40 734.8 0 40 77 28 734.8 0 28 78 44 734.8 0 44 79 39 734.8 0 39 80 60 734.8 0 60 81 55 734.8 0 55 82 62 734.8 0 62 83 69 734.8 0 69 84 51 734.8 0 51 85 123 734.8 0 123 86 182 734.8 0 182 87 63 734.8 0 63 88 39 734.8 0 39 89 51 734.8 0 51 90 47 734.8 0 47 91 67 734.8 0 67 92 143 734.8 0 143 93 89 734.8 0 89 94 79 734.8 0 79 95 109 734.8 0 109 96 88 734.8 0 88 97 72 734.8 0 72 98 286 734.8 0 286 99 275 734.8 0 275 100 124 734.8 0 124 101 95 734.8 0 95 102 105 734.8 0 105 103 143 734.8 0 143 104 89 734.8 0 89 105 45 734.8 0 45 106 35 734.8 0 35 107 37 734.8 0 37 108 28 734.8 0 28 109 45 734.8 0 45 110 53 734.8 0 53 111 132 734.8 0 132 112 102 734.8 0 102 113 103 734.8 0 103 114 71 734.8 0 71 115 54 734.8 0 54 116 96 734.8 0 96 117 98 734.8 0 98 118 93 734.8 0 93 119 62 734.8 0 62 120 64 734.8 0 64 121 59 734.8 0 59 122 51 734.8 0 51 123 90 734.8 0 90 124 167 734.8 0 167 125 133 734.8 0 133 126 57 734.8 0 57 127 66 734.8 0 66 128 61 734.8 0 61 129 52 734.8 0 52 130 68 734.8 0 68 131 106 734.8 0 106 132 117 734.8 0 117 133 144 734.8 0 144 134 81 734.8 0 81 135 84 734.8 0 84 136 66 734.8 0 66 137 95 734.8 0 95 138 149 734.8 0 149 139 135 734.8 0 135 140 91 734.8 0 91 141 75 734.8 0 75 142 128 734.8 0 128 143 195 734.8 0 195 144 196 734.8 0 196 145 109 734.8 0 109 146 72 734.8 0 72 147 109 734.8 0 109 148 96 734.8 0 96 149 86 734.8 0 86 150 91 734.8 0 91 151 101 734.8 0 101 152 89 734.8 0 89 153 90 734.8 0 90 154 66 734.8 0 66 155 61 734.8 0 61 156 67 734.8 0 67 157 85 734.8 0 85 158 131 734.8 0 131 159 167 734.8 0 167 160 106 734.8 0 106 161 81 734.8 0 81 162 159 734.8 0 159 163 113 734.8 0 113 164 79 734.8 0 79 165 73 734.8 0 73 166 80 734.8 0 80 167 105 734.8 0 105 168 113 734.8 0 113 169 147 734.8 0 147 170 192 734.8 0 192 171 200 734.8 0 200 172 134 734.8 0 134 173 87 734.8 0 87 174 47 734.8 0 47 175 49 734.8 0 49 176 74 734.8 0 74 177 139 734.8 0 139 178 190 734.8 0 190 179 152 734.8 0 152 180 125 734.8 0 125 181 72 734.8 0 72 182 69 734.8 0 69 183 135 734.8 0 135 184 210 734.8 0 210 185 131 734.8 0 131 186 93 734.8 0 93 187 191 734.8 0 191 188 198 734.8 0 198 189 140 734.8 0 140 190 88 734.8 0 88 191 65 734.8 0 65 192 89 734.8 0 89 193 118 734.8 0 118 194 138 734.8 0 138 195 113 734.8 0 113 196 82 734.8 0 82 197 83 734.8 0 83 198 187 734.8 0 187 199 180 734.8 0 180 200 78 734.8 0 78 201 50 734.8 0 50 202 51 734.8 0 51 203 59 734.8 0 59 204 112 734.8 0 112 205 101 734.8 0 101 206 116 734.8 0 116 207 115 734.8 0 115 208 91 734.8 0 91 209 66 734.8 0 66 210 130 734.8 0 130 211 212 734.8 0 212 212 180 734.8 0 180 213 169 734.8 0 169 214 968 734.8 0 968 215 1120 734.8 0 1120 216 461 734.8 0 461 217 110 734.8 0 110 218 108 734.8 0 108 219 125 734.8 0 125 220 99 734.8 0 99 221 61 734.8 0 61 222 71 734.8 0 71 223 324 734.8 0 324 224 589 734.8 0 589 225 201 734.8 0 201 226 82 734.8 0 82 227 119 734.8 0 119 228 143 734.8 0 143 229 184 734.8 0 184 230 81 734.8 0 81 231 61 734.8 0 61 232 47 734.8 0 47 233 46 734.8 0 46 234 79 734.8 0 79 235 136 734.8 0 136 236 121 734.8 0 121 237 115 734.8 0 115 238 177 734.8 0 177 239 201 734.8 0 201 240 176 734.8 0 176 241 87 734.8 0 87 242 65 734.8 0 65 243 76 734.8 0 76 244 72 734.8 0 72 245 107 734.8 0 107 246 215 734.8 0 215 247 233 734.8 0 233 248 115 734.8 0 115 249 58 734.8 0 58 250 37 734.8 0 37 251 34 734.8 0 34 252 69 734.8 0 69 253 84 734.8 0 84 254 90 734.8 0 90 255 165 734.8 0 165 256 206 734.8 0 206 257 309 734.8 0 309 258 224 734.8 0 224 259 199 734.8 0 199 260 239 734.8 0 239 261 53 734.8 0 53 262 58 734.8 0 58 263 132 734.8 0 132 264 188 734.8 0 188 265 174 734.8 0 174 266 129 734.8 0 129 267 259 734.8 0 259 268 303 734.8 0 303 269 316 734.8 0 316 270 220 734.8 0 220 271 425 734.8 0 425 272 5125 734.8 0 5125 273 7629 734.8 0 7629 274 4514 734.8 0 4514 275 139 734.8 0 139 276 71 734.8 0 71 277 137 734.8 0 137 278 136 734.8 0 136 279 293 734.8 0 293 280 125 734.8 0 125 281 61 734.8 0 61 282 63 734.8 0 63 283 167 734.8 0 167 284 283 734.8 0 283 285 472 734.8 0 472 286 266 734.8 0 266 287 158 734.8 0 158 288 83 734.8 0 83 289 49 734.8 0 49 290 49 734.8 0 49 291 62 734.8 0 62 292 85 734.8 0 85 293 117 734.8 0 117 294 131 734.8 0 131 295 96 734.8 0 96 296 80 734.8 0 80 297 117 734.8 0 117 298 195 734.8 0 195 299 713 734.8 0 713 300 751 734.8 0 751 301 804 734.8 0 804 Finished in 3.46 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 67,551 Reads with adapters: 3,548 (5.3%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 3,530 (5.2%) Total basepairs processed: 18,046,665 bp Total written (filtered): 906,258 bp (5.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3548 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 39 0.0 3 0 36 2 1 20 2936 0.0 4 2849 83 2 0 2 21 6 0.0 4 1 4 1 22 1 0.0 4 1 23 4 0.0 4 0 0 0 3 1 28 2 0.0 4 0 0 0 0 2 31 1 0.0 4 0 0 0 0 1 36 14 0.0 4 0 0 0 0 14 38 1 0.0 4 0 0 0 1 54 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 126 11 0.0 4 0 0 0 0 11 127 506 0.0 4 0 0 0 0 506 128 3 0.0 4 0 0 0 0 3 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 1 193 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (31 us/read; 1.93 M reads/minute). === Summary === Total reads processed: 3,530 Reads with adapters: 332 (9.4%) Reads written (passing filters): 3,530 (100.0%) Total basepairs processed: 906,258 bp Total written (filtered): 901,177 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 332 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.0% C: 3.9% G: 91.6% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 10 27 0.0 1 1 26 11 298 0.0 1 1 297 29 1 0.0 1 1 209 1 0.0 1 1 259 5 0.0 1 5 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.