This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.30 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 34,637 (1.2%) Reads that were too short: 3,868 (0.1%) Reads written (passing filters): 30,769 (1.0%) Total basepairs processed: 905,907,359 bp Total written (filtered): 7,881,682 bp (0.9%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 34637 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 549 734.8 0 549 7 247 734.8 0 247 8 3195 734.8 0 3195 9 13293 734.8 0 13293 10 39 734.8 0 39 11 14 734.8 0 14 12 22 734.8 0 22 13 19 734.8 0 19 14 22 734.8 0 22 15 15 734.8 0 15 16 18 734.8 0 18 17 14 734.8 0 14 18 19 734.8 0 19 19 6 734.8 0 6 20 16 734.8 0 16 21 22 734.8 0 22 22 17 734.8 0 17 23 21 734.8 0 21 24 15 734.8 0 15 25 16 734.8 0 16 26 17 734.8 0 17 27 20 734.8 0 20 28 14 734.8 0 14 29 24 734.8 0 24 30 48 734.8 0 48 31 56 734.8 0 56 32 19 734.8 0 19 33 14 734.8 0 14 34 21 734.8 0 21 35 29 734.8 0 29 36 29 734.8 0 29 37 19 734.8 0 19 38 17 734.8 0 17 39 18 734.8 0 18 40 21 734.8 0 21 41 17 734.8 0 17 42 18 734.8 0 18 43 19 734.8 0 19 44 24 734.8 0 24 45 15 734.8 0 15 46 12 734.8 0 12 47 13 734.8 0 13 48 19 734.8 0 19 49 16 734.8 0 16 50 23 734.8 0 23 51 24 734.8 0 24 52 14 734.8 0 14 53 91 734.8 0 91 54 4388 734.8 0 4388 55 301 734.8 0 301 56 135 734.8 0 135 57 13 734.8 0 13 58 22 734.8 0 22 59 12 734.8 0 12 60 13 734.8 0 13 61 20 734.8 0 20 62 16 734.8 0 16 63 17 734.8 0 17 64 25 734.8 0 25 65 17 734.8 0 17 66 16 734.8 0 16 67 28 734.8 0 28 68 30 734.8 0 30 69 26 734.8 0 26 70 21 734.8 0 21 71 29 734.8 0 29 72 71 734.8 0 71 73 758 734.8 0 758 74 1708 734.8 0 1708 75 159 734.8 0 159 76 13 734.8 0 13 77 13 734.8 0 13 78 14 734.8 0 14 79 11 734.8 0 11 80 22 734.8 0 22 81 13 734.8 0 13 82 25 734.8 0 25 83 26 734.8 0 26 84 22 734.8 0 22 85 27 734.8 0 27 86 24 734.8 0 24 87 30 734.8 0 30 88 24 734.8 0 24 89 20 734.8 0 20 90 19 734.8 0 19 91 12 734.8 0 12 92 14 734.8 0 14 93 12 734.8 0 12 94 22 734.8 0 22 95 24 734.8 0 24 96 18 734.8 0 18 97 16 734.8 0 16 98 15 734.8 0 15 99 19 734.8 0 19 100 18 734.8 0 18 101 15 734.8 0 15 102 15 734.8 0 15 103 13 734.8 0 13 104 19 734.8 0 19 105 23 734.8 0 23 106 16 734.8 0 16 107 18 734.8 0 18 108 24 734.8 0 24 109 49 734.8 0 49 110 32 734.8 0 32 111 14 734.8 0 14 112 15 734.8 0 15 113 16 734.8 0 16 114 15 734.8 0 15 115 32 734.8 0 32 116 18 734.8 0 18 117 13 734.8 0 13 118 10 734.8 0 10 119 15 734.8 0 15 120 15 734.8 0 15 121 27 734.8 0 27 122 47 734.8 0 47 123 9 734.8 0 9 124 17 734.8 0 17 125 22 734.8 0 22 126 28 734.8 0 28 127 12 734.8 0 12 128 22 734.8 0 22 129 32 734.8 0 32 130 34 734.8 0 34 131 25 734.8 0 25 132 14 734.8 0 14 133 26 734.8 0 26 134 24 734.8 0 24 135 27 734.8 0 27 136 54 734.8 0 54 137 122 734.8 0 122 138 45 734.8 0 45 139 16 734.8 0 16 140 27 734.8 0 27 141 26 734.8 0 26 142 21 734.8 0 21 143 21 734.8 0 21 144 18 734.8 0 18 145 35 734.8 0 35 146 43 734.8 0 43 147 78 734.8 0 78 148 73 734.8 0 73 149 71 734.8 0 71 150 38 734.8 0 38 151 9 734.8 0 9 152 17 734.8 0 17 153 20 734.8 0 20 154 42 734.8 0 42 155 40 734.8 0 40 156 47 734.8 0 47 157 31 734.8 0 31 158 113 734.8 0 113 159 67 734.8 0 67 160 68 734.8 0 68 161 33 734.8 0 33 162 56 734.8 0 56 163 27 734.8 0 27 164 23 734.8 0 23 165 37 734.8 0 37 166 53 734.8 0 53 167 54 734.8 0 54 168 26 734.8 0 26 169 29 734.8 0 29 170 88 734.8 0 88 171 35 734.8 0 35 172 21 734.8 0 21 173 34 734.8 0 34 174 307 734.8 0 307 175 237 734.8 0 237 176 80 734.8 0 80 177 25 734.8 0 25 178 23 734.8 0 23 179 11 734.8 0 11 180 30 734.8 0 30 181 53 734.8 0 53 182 56 734.8 0 56 183 44 734.8 0 44 184 38 734.8 0 38 185 39 734.8 0 39 186 64 734.8 0 64 187 354 734.8 0 354 188 166 734.8 0 166 189 34 734.8 0 34 190 27 734.8 0 27 191 17 734.8 0 17 192 30 734.8 0 30 193 22 734.8 0 22 194 27 734.8 0 27 195 20 734.8 0 20 196 17 734.8 0 17 197 21 734.8 0 21 198 13 734.8 0 13 199 18 734.8 0 18 200 15 734.8 0 15 201 22 734.8 0 22 202 10 734.8 0 10 203 21 734.8 0 21 204 62 734.8 0 62 205 50 734.8 0 50 206 48 734.8 0 48 207 19 734.8 0 19 208 24 734.8 0 24 209 20 734.8 0 20 210 21 734.8 0 21 211 30 734.8 0 30 212 23 734.8 0 23 213 25 734.8 0 25 214 21 734.8 0 21 215 26 734.8 0 26 216 23 734.8 0 23 217 30 734.8 0 30 218 27 734.8 0 27 219 19 734.8 0 19 220 34 734.8 0 34 221 20 734.8 0 20 222 23 734.8 0 23 223 32 734.8 0 32 224 28 734.8 0 28 225 27 734.8 0 27 226 28 734.8 0 28 227 72 734.8 0 72 228 49 734.8 0 49 229 33 734.8 0 33 230 33 734.8 0 33 231 30 734.8 0 30 232 37 734.8 0 37 233 23 734.8 0 23 234 22 734.8 0 22 235 26 734.8 0 26 236 23 734.8 0 23 237 34 734.8 0 34 238 49 734.8 0 49 239 21 734.8 0 21 240 16 734.8 0 16 241 27 734.8 0 27 242 18 734.8 0 18 243 11 734.8 0 11 244 24 734.8 0 24 245 18 734.8 0 18 246 25 734.8 0 25 247 27 734.8 0 27 248 23 734.8 0 23 249 36 734.8 0 36 250 17 734.8 0 17 251 70 734.8 0 70 252 218 734.8 0 218 253 158 734.8 0 158 254 100 734.8 0 100 255 39 734.8 0 39 256 15 734.8 0 15 257 18 734.8 0 18 258 39 734.8 0 39 259 21 734.8 0 21 260 51 734.8 0 51 261 100 734.8 0 100 262 142 734.8 0 142 263 92 734.8 0 92 264 68 734.8 0 68 265 22 734.8 0 22 266 17 734.8 0 17 267 26 734.8 0 26 268 29 734.8 0 29 269 27 734.8 0 27 270 19 734.8 0 19 271 13 734.8 0 13 272 35 734.8 0 35 273 44 734.8 0 44 274 65 734.8 0 65 275 25 734.8 0 25 276 29 734.8 0 29 277 36 734.8 0 36 278 65 734.8 0 65 279 27 734.8 0 27 280 26 734.8 0 26 281 54 734.8 0 54 282 60 734.8 0 60 283 24 734.8 0 24 284 20 734.8 0 20 285 82 734.8 0 82 286 43 734.8 0 43 287 22 734.8 0 22 288 33 734.8 0 33 289 19 734.8 0 19 290 27 734.8 0 27 291 39 734.8 0 39 292 36 734.8 0 36 293 26 734.8 0 26 294 39 734.8 0 39 295 72 734.8 0 72 296 19 734.8 0 19 297 34 734.8 0 34 298 29 734.8 0 29 299 41 734.8 0 41 300 66 734.8 0 66 301 62 734.8 0 62 Finished in 1.34 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 30,769 Reads with adapters: 3,035 (9.9%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 3,026 (9.8%) Total basepairs processed: 7,881,682 bp Total written (filtered): 834,330 bp (10.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3035 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 33 0.0 3 0 33 17 2966 0.0 3 2923 42 1 18 11 0.0 3 2 5 4 19 2 0.0 3 0 0 1 1 20 3 0.0 3 0 0 0 3 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 3,026 Reads with adapters: 171 (5.7%) Reads written (passing filters): 3,026 (100.0%) Total basepairs processed: 834,330 bp Total written (filtered): 833,813 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 171 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 84.8% C: 4.7% G: 7.0% T: 3.5% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 169 47.3 0 169 5 2 3.0 0 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.