This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.52 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 316,845 (10.5%) Reads that were too short: 37,437 (1.2%) Reads written (passing filters): 279,408 (9.3%) Total basepairs processed: 905,907,359 bp Total written (filtered): 74,820,337 bp (8.3%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 316845 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1628 734.8 0 1628 7 218457 734.8 0 218457 8 10826 734.8 0 10826 9 31 734.8 0 31 10 17 734.8 0 17 11 12 734.8 0 12 12 43 734.8 0 43 13 90 734.8 0 90 14 107 734.8 0 107 15 52 734.8 0 52 16 14 734.8 0 14 17 4 734.8 0 4 18 6 734.8 0 6 19 8 734.8 0 8 20 5 734.8 0 5 21 10 734.8 0 10 22 4 734.8 0 4 23 7 734.8 0 7 24 16 734.8 0 16 25 621 734.8 0 621 26 76 734.8 0 76 27 7 734.8 0 7 28 4 734.8 0 4 29 3 734.8 0 3 30 7 734.8 0 7 31 5 734.8 0 5 32 9 734.8 0 9 33 10 734.8 0 10 34 8 734.8 0 8 35 7 734.8 0 7 36 8 734.8 0 8 37 6 734.8 0 6 38 4 734.8 0 4 39 7 734.8 0 7 40 7 734.8 0 7 41 9 734.8 0 9 42 35 734.8 0 35 43 15 734.8 0 15 44 8 734.8 0 8 45 12 734.8 0 12 46 12 734.8 0 12 47 7 734.8 0 7 48 13 734.8 0 13 49 12 734.8 0 12 50 14 734.8 0 14 51 5 734.8 0 5 52 3 734.8 0 3 53 17 734.8 0 17 54 8 734.8 0 8 55 11 734.8 0 11 56 24 734.8 0 24 57 13 734.8 0 13 58 12 734.8 0 12 59 10 734.8 0 10 60 25 734.8 0 25 61 31 734.8 0 31 62 49 734.8 0 49 63 15 734.8 0 15 64 5 734.8 0 5 65 9 734.8 0 9 66 10 734.8 0 10 67 10 734.8 0 10 68 9 734.8 0 9 69 10 734.8 0 10 70 13 734.8 0 13 71 78 734.8 0 78 72 16 734.8 0 16 73 9 734.8 0 9 74 21 734.8 0 21 75 6 734.8 0 6 76 13 734.8 0 13 77 15 734.8 0 15 78 14 734.8 0 14 79 26 734.8 0 26 80 45 734.8 0 45 81 101 734.8 0 101 82 372 734.8 0 372 83 343 734.8 0 343 84 146 734.8 0 146 85 108 734.8 0 108 86 68 734.8 0 68 87 80 734.8 0 80 88 379 734.8 0 379 89 238 734.8 0 238 90 188 734.8 0 188 91 95 734.8 0 95 92 58 734.8 0 58 93 130 734.8 0 130 94 155 734.8 0 155 95 106 734.8 0 106 96 137 734.8 0 137 97 105 734.8 0 105 98 156 734.8 0 156 99 246 734.8 0 246 100 111 734.8 0 111 101 94 734.8 0 94 102 224 734.8 0 224 103 383 734.8 0 383 104 173 734.8 0 173 105 123 734.8 0 123 106 131 734.8 0 131 107 167 734.8 0 167 108 62 734.8 0 62 109 38 734.8 0 38 110 118 734.8 0 118 111 264 734.8 0 264 112 326 734.8 0 326 113 170 734.8 0 170 114 209 734.8 0 209 115 143 734.8 0 143 116 87 734.8 0 87 117 83 734.8 0 83 118 234 734.8 0 234 119 312 734.8 0 312 120 354 734.8 0 354 121 205 734.8 0 205 122 205 734.8 0 205 123 287 734.8 0 287 124 247 734.8 0 247 125 154 734.8 0 154 126 226 734.8 0 226 127 198 734.8 0 198 128 160 734.8 0 160 129 203 734.8 0 203 130 183 734.8 0 183 131 154 734.8 0 154 132 199 734.8 0 199 133 213 734.8 0 213 134 258 734.8 0 258 135 200 734.8 0 200 136 153 734.8 0 153 137 192 734.8 0 192 138 225 734.8 0 225 139 443 734.8 0 443 140 538 734.8 0 538 141 374 734.8 0 374 142 203 734.8 0 203 143 207 734.8 0 207 144 290 734.8 0 290 145 241 734.8 0 241 146 235 734.8 0 235 147 250 734.8 0 250 148 251 734.8 0 251 149 183 734.8 0 183 150 184 734.8 0 184 151 192 734.8 0 192 152 264 734.8 0 264 153 330 734.8 0 330 154 242 734.8 0 242 155 209 734.8 0 209 156 201 734.8 0 201 157 227 734.8 0 227 158 277 734.8 0 277 159 339 734.8 0 339 160 868 734.8 0 868 161 898 734.8 0 898 162 511 734.8 0 511 163 243 734.8 0 243 164 221 734.8 0 221 165 205 734.8 0 205 166 163 734.8 0 163 167 195 734.8 0 195 168 295 734.8 0 295 169 304 734.8 0 304 170 243 734.8 0 243 171 179 734.8 0 179 172 244 734.8 0 244 173 328 734.8 0 328 174 211 734.8 0 211 175 217 734.8 0 217 176 240 734.8 0 240 177 285 734.8 0 285 178 241 734.8 0 241 179 278 734.8 0 278 180 367 734.8 0 367 181 323 734.8 0 323 182 461 734.8 0 461 183 325 734.8 0 325 184 366 734.8 0 366 185 460 734.8 0 460 186 538 734.8 0 538 187 6223 734.8 0 6223 188 10037 734.8 0 10037 189 1711 734.8 0 1711 190 326 734.8 0 326 191 214 734.8 0 214 192 177 734.8 0 177 193 230 734.8 0 230 194 175 734.8 0 175 195 196 734.8 0 196 196 316 734.8 0 316 197 315 734.8 0 315 198 232 734.8 0 232 199 205 734.8 0 205 200 248 734.8 0 248 201 440 734.8 0 440 202 582 734.8 0 582 203 275 734.8 0 275 204 249 734.8 0 249 205 288 734.8 0 288 206 437 734.8 0 437 207 394 734.8 0 394 208 364 734.8 0 364 209 274 734.8 0 274 210 313 734.8 0 313 211 358 734.8 0 358 212 296 734.8 0 296 213 288 734.8 0 288 214 293 734.8 0 293 215 282 734.8 0 282 216 277 734.8 0 277 217 232 734.8 0 232 218 306 734.8 0 306 219 431 734.8 0 431 220 353 734.8 0 353 221 388 734.8 0 388 222 396 734.8 0 396 223 290 734.8 0 290 224 245 734.8 0 245 225 260 734.8 0 260 226 297 734.8 0 297 227 290 734.8 0 290 228 263 734.8 0 263 229 364 734.8 0 364 230 527 734.8 0 527 231 471 734.8 0 471 232 336 734.8 0 336 233 320 734.8 0 320 234 341 734.8 0 341 235 310 734.8 0 310 236 169 734.8 0 169 237 254 734.8 0 254 238 340 734.8 0 340 239 417 734.8 0 417 240 575 734.8 0 575 241 484 734.8 0 484 242 456 734.8 0 456 243 279 734.8 0 279 244 281 734.8 0 281 245 282 734.8 0 282 246 319 734.8 0 319 247 181 734.8 0 181 248 143 734.8 0 143 249 141 734.8 0 141 250 381 734.8 0 381 251 469 734.8 0 469 252 307 734.8 0 307 253 227 734.8 0 227 254 268 734.8 0 268 255 275 734.8 0 275 256 308 734.8 0 308 257 281 734.8 0 281 258 299 734.8 0 299 259 225 734.8 0 225 260 260 734.8 0 260 261 277 734.8 0 277 262 381 734.8 0 381 263 288 734.8 0 288 264 339 734.8 0 339 265 255 734.8 0 255 266 353 734.8 0 353 267 246 734.8 0 246 268 216 734.8 0 216 269 245 734.8 0 245 270 457 734.8 0 457 271 364 734.8 0 364 272 291 734.8 0 291 273 291 734.8 0 291 274 361 734.8 0 361 275 563 734.8 0 563 276 469 734.8 0 469 277 431 734.8 0 431 278 473 734.8 0 473 279 466 734.8 0 466 280 258 734.8 0 258 281 274 734.8 0 274 282 429 734.8 0 429 283 554 734.8 0 554 284 356 734.8 0 356 285 371 734.8 0 371 286 290 734.8 0 290 287 245 734.8 0 245 288 204 734.8 0 204 289 403 734.8 0 403 290 450 734.8 0 450 291 333 734.8 0 333 292 401 734.8 0 401 293 317 734.8 0 317 294 299 734.8 0 299 295 382 734.8 0 382 296 423 734.8 0 423 297 333 734.8 0 333 298 394 734.8 0 394 299 471 734.8 0 471 300 545 734.8 0 545 301 3928 734.8 0 3928 Finished in 11.10 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 279,408 Reads with adapters: 219,274 (78.5%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 219,250 (78.5%) Total basepairs processed: 74,820,337 bp Total written (filtered): 60,730,013 bp (81.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 219274 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 54 0.0 3 0 0 52 2 16 1631 0.0 3 0 1603 28 17 216614 0.0 3 214336 2234 34 10 18 584 0.0 3 30 535 16 3 19 86 0.0 3 0 0 77 9 20 251 0.0 3 0 1 0 250 21 2 0.0 3 1 0 0 1 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 27 2 0.0 3 1 0 0 1 30 1 0.0 3 1 33 1 0.0 3 0 0 0 1 36 2 0.0 3 1 0 0 1 40 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 141 6 0.0 3 0 0 0 6 156 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 7.93 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 219,250 Reads with adapters: 26,927 (12.3%) Reads written (passing filters): 219,250 (100.0%) Total basepairs processed: 60,730,013 bp Total written (filtered): 60,649,204 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 26927 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 47.6% C: 49.7% G: 2.2% T: 0.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 26907 3425.8 0 26907 4 16 856.4 0 16 5 2 214.1 0 2 7 2 13.4 0 2