This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.93 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 329,492 (10.9%) Reads that were too short: 23,361 (0.8%) Reads written (passing filters): 306,131 (10.2%) Total basepairs processed: 905,907,359 bp Total written (filtered): 87,832,555 bp (9.7%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 329492 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1639 734.8 0 1639 7 2702 734.8 0 2702 8 280526 734.8 0 280526 9 3737 734.8 0 3737 10 74 734.8 0 74 11 60 734.8 0 60 12 75 734.8 0 75 13 64 734.8 0 64 14 63 734.8 0 63 15 64 734.8 0 64 16 81 734.8 0 81 17 55 734.8 0 55 18 74 734.8 0 74 19 76 734.8 0 76 20 72 734.8 0 72 21 87 734.8 0 87 22 60 734.8 0 60 23 58 734.8 0 58 24 70 734.8 0 70 25 64 734.8 0 64 26 69 734.8 0 69 27 68 734.8 0 68 28 47 734.8 0 47 29 78 734.8 0 78 30 88 734.8 0 88 31 56 734.8 0 56 32 53 734.8 0 53 33 65 734.8 0 65 34 83 734.8 0 83 35 76 734.8 0 76 36 69 734.8 0 69 37 72 734.8 0 72 38 54 734.8 0 54 39 50 734.8 0 50 40 78 734.8 0 78 41 76 734.8 0 76 42 67 734.8 0 67 43 49 734.8 0 49 44 75 734.8 0 75 45 112 734.8 0 112 46 67 734.8 0 67 47 74 734.8 0 74 48 55 734.8 0 55 49 69 734.8 0 69 50 62 734.8 0 62 51 48 734.8 0 48 52 85 734.8 0 85 53 77 734.8 0 77 54 61 734.8 0 61 55 63 734.8 0 63 56 68 734.8 0 68 57 53 734.8 0 53 58 63 734.8 0 63 59 114 734.8 0 114 60 142 734.8 0 142 61 96 734.8 0 96 62 87 734.8 0 87 63 72 734.8 0 72 64 61 734.8 0 61 65 62 734.8 0 62 66 54 734.8 0 54 67 67 734.8 0 67 68 84 734.8 0 84 69 71 734.8 0 71 70 100 734.8 0 100 71 82 734.8 0 82 72 103 734.8 0 103 73 65 734.8 0 65 74 53 734.8 0 53 75 70 734.8 0 70 76 117 734.8 0 117 77 223 734.8 0 223 78 179 734.8 0 179 79 84 734.8 0 84 80 94 734.8 0 94 81 106 734.8 0 106 82 97 734.8 0 97 83 80 734.8 0 80 84 97 734.8 0 97 85 102 734.8 0 102 86 80 734.8 0 80 87 81 734.8 0 81 88 74 734.8 0 74 89 76 734.8 0 76 90 76 734.8 0 76 91 84 734.8 0 84 92 100 734.8 0 100 93 131 734.8 0 131 94 129 734.8 0 129 95 94 734.8 0 94 96 89 734.8 0 89 97 89 734.8 0 89 98 140 734.8 0 140 99 80 734.8 0 80 100 86 734.8 0 86 101 63 734.8 0 63 102 69 734.8 0 69 103 77 734.8 0 77 104 72 734.8 0 72 105 68 734.8 0 68 106 97 734.8 0 97 107 67 734.8 0 67 108 50 734.8 0 50 109 64 734.8 0 64 110 83 734.8 0 83 111 77 734.8 0 77 112 108 734.8 0 108 113 104 734.8 0 104 114 91 734.8 0 91 115 79 734.8 0 79 116 95 734.8 0 95 117 114 734.8 0 114 118 102 734.8 0 102 119 77 734.8 0 77 120 75 734.8 0 75 121 90 734.8 0 90 122 79 734.8 0 79 123 52 734.8 0 52 124 73 734.8 0 73 125 77 734.8 0 77 126 86 734.8 0 86 127 92 734.8 0 92 128 124 734.8 0 124 129 111 734.8 0 111 130 142 734.8 0 142 131 102 734.8 0 102 132 131 734.8 0 131 133 130 734.8 0 130 134 117 734.8 0 117 135 94 734.8 0 94 136 90 734.8 0 90 137 79 734.8 0 79 138 124 734.8 0 124 139 135 734.8 0 135 140 91 734.8 0 91 141 88 734.8 0 88 142 107 734.8 0 107 143 154 734.8 0 154 144 138 734.8 0 138 145 229 734.8 0 229 146 78 734.8 0 78 147 110 734.8 0 110 148 95 734.8 0 95 149 95 734.8 0 95 150 84 734.8 0 84 151 118 734.8 0 118 152 86 734.8 0 86 153 97 734.8 0 97 154 97 734.8 0 97 155 114 734.8 0 114 156 105 734.8 0 105 157 156 734.8 0 156 158 115 734.8 0 115 159 99 734.8 0 99 160 106 734.8 0 106 161 78 734.8 0 78 162 113 734.8 0 113 163 89 734.8 0 89 164 94 734.8 0 94 165 91 734.8 0 91 166 88 734.8 0 88 167 85 734.8 0 85 168 83 734.8 0 83 169 83 734.8 0 83 170 96 734.8 0 96 171 105 734.8 0 105 172 91 734.8 0 91 173 79 734.8 0 79 174 88 734.8 0 88 175 94 734.8 0 94 176 83 734.8 0 83 177 92 734.8 0 92 178 73 734.8 0 73 179 94 734.8 0 94 180 79 734.8 0 79 181 80 734.8 0 80 182 73 734.8 0 73 183 88 734.8 0 88 184 105 734.8 0 105 185 73 734.8 0 73 186 98 734.8 0 98 187 102 734.8 0 102 188 92 734.8 0 92 189 83 734.8 0 83 190 112 734.8 0 112 191 210 734.8 0 210 192 143 734.8 0 143 193 109 734.8 0 109 194 120 734.8 0 120 195 95 734.8 0 95 196 97 734.8 0 97 197 270 734.8 0 270 198 289 734.8 0 289 199 119 734.8 0 119 200 84 734.8 0 84 201 87 734.8 0 87 202 53 734.8 0 53 203 71 734.8 0 71 204 99 734.8 0 99 205 120 734.8 0 120 206 92 734.8 0 92 207 88 734.8 0 88 208 69 734.8 0 69 209 75 734.8 0 75 210 100 734.8 0 100 211 103 734.8 0 103 212 96 734.8 0 96 213 110 734.8 0 110 214 101 734.8 0 101 215 118 734.8 0 118 216 84 734.8 0 84 217 82 734.8 0 82 218 97 734.8 0 97 219 91 734.8 0 91 220 86 734.8 0 86 221 85 734.8 0 85 222 87 734.8 0 87 223 113 734.8 0 113 224 357 734.8 0 357 225 98 734.8 0 98 226 86 734.8 0 86 227 95 734.8 0 95 228 74 734.8 0 74 229 67 734.8 0 67 230 101 734.8 0 101 231 131 734.8 0 131 232 137 734.8 0 137 233 104 734.8 0 104 234 108 734.8 0 108 235 89 734.8 0 89 236 97 734.8 0 97 237 111 734.8 0 111 238 126 734.8 0 126 239 110 734.8 0 110 240 133 734.8 0 133 241 104 734.8 0 104 242 90 734.8 0 90 243 91 734.8 0 91 244 86 734.8 0 86 245 125 734.8 0 125 246 154 734.8 0 154 247 117 734.8 0 117 248 83 734.8 0 83 249 89 734.8 0 89 250 89 734.8 0 89 251 55 734.8 0 55 252 61 734.8 0 61 253 110 734.8 0 110 254 161 734.8 0 161 255 130 734.8 0 130 256 263 734.8 0 263 257 321 734.8 0 321 258 195 734.8 0 195 259 94 734.8 0 94 260 97 734.8 0 97 261 93 734.8 0 93 262 93 734.8 0 93 263 102 734.8 0 102 264 125 734.8 0 125 265 533 734.8 0 533 266 449 734.8 0 449 267 279 734.8 0 279 268 203 734.8 0 203 269 79 734.8 0 79 270 70 734.8 0 70 271 69 734.8 0 69 272 109 734.8 0 109 273 108 734.8 0 108 274 85 734.8 0 85 275 107 734.8 0 107 276 76 734.8 0 76 277 88 734.8 0 88 278 76 734.8 0 76 279 96 734.8 0 96 280 101 734.8 0 101 281 241 734.8 0 241 282 130 734.8 0 130 283 113 734.8 0 113 284 158 734.8 0 158 285 184 734.8 0 184 286 137 734.8 0 137 287 150 734.8 0 150 288 192 734.8 0 192 289 221 734.8 0 221 290 235 734.8 0 235 291 277 734.8 0 277 292 356 734.8 0 356 293 509 734.8 0 509 294 406 734.8 0 406 295 394 734.8 0 394 296 415 734.8 0 415 297 462 734.8 0 462 298 2314 734.8 0 2314 299 2141 734.8 0 2141 300 4250 734.8 0 4250 301 576 734.8 0 576 Finished in 15.66 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 306,131 Reads with adapters: 6,006 (2.0%) Reads that were too short: 66 (0.0%) Reads written (passing filters): 5,940 (1.9%) Total basepairs processed: 87,832,555 bp Total written (filtered): 1,350,740 bp (1.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6006 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 7 0.0 3 0 0 4 2 1 19 51 0.0 3 0 44 5 2 20 3356 0.0 4 3147 179 17 8 5 21 15 0.0 4 2 11 1 1 22 1 0.0 4 0 0 1 23 4 0.0 4 0 0 0 3 1 27 2 0.0 4 0 0 0 0 2 28 2 0.0 4 2 34 1 0.0 4 0 0 0 0 1 70 2 0.0 4 0 0 0 2 74 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 5 0.0 4 0 0 0 0 5 126 24 0.0 4 0 0 0 0 24 127 2453 0.0 4 0 0 0 0 2453 128 5 0.0 4 0 0 0 0 5 131 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 152 3 0.0 4 0 0 0 0 3 153 3 0.0 4 0 0 0 0 3 194 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 224 2 0.0 4 0 0 0 0 2 225 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 246 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 4 0.0 4 0 0 0 0 4 258 2 0.0 4 0 0 0 0 2 260 1 0.0 4 0 0 0 0 1 262 4 0.0 4 0 0 0 0 4 263 2 0.0 4 0 0 0 0 2 264 5 0.0 4 0 0 0 0 5 265 2 0.0 4 0 0 0 0 2 268 1 0.0 4 0 0 0 0 1 281 2 0.0 4 0 0 0 0 2 282 4 0.0 4 0 0 0 0 4 283 2 0.0 4 0 0 0 0 2 285 3 0.0 4 0 0 0 0 3 286 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 291 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.18 s (30 us/read; 1.98 M reads/minute). === Summary === Total reads processed: 5,940 Reads with adapters: 213 (3.6%) Reads written (passing filters): 5,940 (100.0%) Total basepairs processed: 1,350,740 bp Total written (filtered): 1,347,324 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 213 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.4% C: 7.0% G: 82.2% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 92.8 0 1 9 58 0.0 0 7 51 10 86 0.0 1 7 79 11 61 0.0 1 2 59 80 1 0.0 1 1 126 1 0.0 1 1 201 2 0.0 1 2 238 1 0.0 1 1 257 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.