This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.01 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 97,617 (3.2%) Reads that were too short: 24,056 (0.8%) Reads written (passing filters): 73,561 (2.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 17,085,554 bp (1.9%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 97617 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1294 734.8 0 1294 7 34713 734.8 0 34713 8 2373 734.8 0 2373 9 3258 734.8 0 3258 10 13 734.8 0 13 11 14 734.8 0 14 12 15 734.8 0 15 13 20 734.8 0 20 14 16 734.8 0 16 15 15 734.8 0 15 16 12 734.8 0 12 17 17 734.8 0 17 18 12 734.8 0 12 19 25 734.8 0 25 20 12 734.8 0 12 21 25 734.8 0 25 22 18 734.8 0 18 23 18 734.8 0 18 24 11 734.8 0 11 25 18 734.8 0 18 26 9 734.8 0 9 27 12 734.8 0 12 28 28 734.8 0 28 29 20 734.8 0 20 30 14 734.8 0 14 31 22 734.8 0 22 32 11 734.8 0 11 33 18 734.8 0 18 34 52 734.8 0 52 35 21 734.8 0 21 36 12 734.8 0 12 37 21 734.8 0 21 38 45 734.8 0 45 39 49 734.8 0 49 40 12 734.8 0 12 41 27 734.8 0 27 42 88 734.8 0 88 43 116 734.8 0 116 44 28 734.8 0 28 45 12 734.8 0 12 46 16 734.8 0 16 47 17 734.8 0 17 48 9 734.8 0 9 49 13 734.8 0 13 50 23 734.8 0 23 51 21 734.8 0 21 52 24 734.8 0 24 53 118 734.8 0 118 54 77 734.8 0 77 55 28 734.8 0 28 56 12 734.8 0 12 57 32 734.8 0 32 58 13 734.8 0 13 59 9 734.8 0 9 60 16 734.8 0 16 61 21 734.8 0 21 62 19 734.8 0 19 63 19 734.8 0 19 64 11 734.8 0 11 65 24 734.8 0 24 66 16 734.8 0 16 67 21 734.8 0 21 68 10 734.8 0 10 69 19 734.8 0 19 70 25 734.8 0 25 71 76 734.8 0 76 72 19 734.8 0 19 73 21 734.8 0 21 74 11 734.8 0 11 75 11 734.8 0 11 76 9 734.8 0 9 77 19 734.8 0 19 78 19 734.8 0 19 79 19 734.8 0 19 80 17 734.8 0 17 81 34 734.8 0 34 82 37 734.8 0 37 83 34 734.8 0 34 84 36 734.8 0 36 85 22 734.8 0 22 86 20 734.8 0 20 87 39 734.8 0 39 88 35 734.8 0 35 89 34 734.8 0 34 90 23 734.8 0 23 91 77 734.8 0 77 92 51 734.8 0 51 93 105 734.8 0 105 94 108 734.8 0 108 95 73 734.8 0 73 96 34 734.8 0 34 97 30 734.8 0 30 98 49 734.8 0 49 99 42 734.8 0 42 100 31 734.8 0 31 101 57 734.8 0 57 102 78 734.8 0 78 103 105 734.8 0 105 104 119 734.8 0 119 105 69 734.8 0 69 106 50 734.8 0 50 107 128 734.8 0 128 108 39 734.8 0 39 109 40 734.8 0 40 110 48 734.8 0 48 111 167 734.8 0 167 112 179 734.8 0 179 113 216 734.8 0 216 114 174 734.8 0 174 115 107 734.8 0 107 116 52 734.8 0 52 117 44 734.8 0 44 118 47 734.8 0 47 119 53 734.8 0 53 120 79 734.8 0 79 121 51 734.8 0 51 122 64 734.8 0 64 123 92 734.8 0 92 124 149 734.8 0 149 125 651 734.8 0 651 126 806 734.8 0 806 127 422 734.8 0 422 128 62 734.8 0 62 129 100 734.8 0 100 130 136 734.8 0 136 131 78 734.8 0 78 132 118 734.8 0 118 133 146 734.8 0 146 134 173 734.8 0 173 135 77 734.8 0 77 136 148 734.8 0 148 137 102 734.8 0 102 138 135 734.8 0 135 139 144 734.8 0 144 140 126 734.8 0 126 141 95 734.8 0 95 142 85 734.8 0 85 143 117 734.8 0 117 144 270 734.8 0 270 145 270 734.8 0 270 146 271 734.8 0 271 147 156 734.8 0 156 148 115 734.8 0 115 149 154 734.8 0 154 150 276 734.8 0 276 151 616 734.8 0 616 152 278 734.8 0 278 153 185 734.8 0 185 154 190 734.8 0 190 155 672 734.8 0 672 156 301 734.8 0 301 157 330 734.8 0 330 158 366 734.8 0 366 159 522 734.8 0 522 160 4270 734.8 0 4270 161 5145 734.8 0 5145 162 1777 734.8 0 1777 163 149 734.8 0 149 164 303 734.8 0 303 165 278 734.8 0 278 166 173 734.8 0 173 167 105 734.8 0 105 168 100 734.8 0 100 169 164 734.8 0 164 170 148 734.8 0 148 171 122 734.8 0 122 172 132 734.8 0 132 173 116 734.8 0 116 174 117 734.8 0 117 175 138 734.8 0 138 176 128 734.8 0 128 177 199 734.8 0 199 178 152 734.8 0 152 179 222 734.8 0 222 180 589 734.8 0 589 181 620 734.8 0 620 182 590 734.8 0 590 183 186 734.8 0 186 184 138 734.8 0 138 185 137 734.8 0 137 186 332 734.8 0 332 187 182 734.8 0 182 188 129 734.8 0 129 189 166 734.8 0 166 190 211 734.8 0 211 191 178 734.8 0 178 192 94 734.8 0 94 193 67 734.8 0 67 194 74 734.8 0 74 195 78 734.8 0 78 196 114 734.8 0 114 197 99 734.8 0 99 198 87 734.8 0 87 199 111 734.8 0 111 200 103 734.8 0 103 201 174 734.8 0 174 202 98 734.8 0 98 203 84 734.8 0 84 204 91 734.8 0 91 205 190 734.8 0 190 206 115 734.8 0 115 207 97 734.8 0 97 208 113 734.8 0 113 209 112 734.8 0 112 210 132 734.8 0 132 211 184 734.8 0 184 212 824 734.8 0 824 213 1068 734.8 0 1068 214 333 734.8 0 333 215 145 734.8 0 145 216 245 734.8 0 245 217 235 734.8 0 235 218 242 734.8 0 242 219 145 734.8 0 145 220 134 734.8 0 134 221 208 734.8 0 208 222 184 734.8 0 184 223 153 734.8 0 153 224 164 734.8 0 164 225 305 734.8 0 305 226 194 734.8 0 194 227 216 734.8 0 216 228 159 734.8 0 159 229 222 734.8 0 222 230 172 734.8 0 172 231 144 734.8 0 144 232 110 734.8 0 110 233 146 734.8 0 146 234 141 734.8 0 141 235 125 734.8 0 125 236 155 734.8 0 155 237 238 734.8 0 238 238 167 734.8 0 167 239 343 734.8 0 343 240 287 734.8 0 287 241 303 734.8 0 303 242 193 734.8 0 193 243 321 734.8 0 321 244 234 734.8 0 234 245 340 734.8 0 340 246 258 734.8 0 258 247 225 734.8 0 225 248 151 734.8 0 151 249 133 734.8 0 133 250 229 734.8 0 229 251 131 734.8 0 131 252 105 734.8 0 105 253 132 734.8 0 132 254 302 734.8 0 302 255 310 734.8 0 310 256 498 734.8 0 498 257 496 734.8 0 496 258 284 734.8 0 284 259 156 734.8 0 156 260 251 734.8 0 251 261 213 734.8 0 213 262 202 734.8 0 202 263 182 734.8 0 182 264 237 734.8 0 237 265 210 734.8 0 210 266 616 734.8 0 616 267 444 734.8 0 444 268 290 734.8 0 290 269 206 734.8 0 206 270 265 734.8 0 265 271 293 734.8 0 293 272 378 734.8 0 378 273 906 734.8 0 906 274 592 734.8 0 592 275 311 734.8 0 311 276 299 734.8 0 299 277 334 734.8 0 334 278 282 734.8 0 282 279 204 734.8 0 204 280 206 734.8 0 206 281 315 734.8 0 315 282 470 734.8 0 470 283 218 734.8 0 218 284 218 734.8 0 218 285 249 734.8 0 249 286 163 734.8 0 163 287 300 734.8 0 300 288 291 734.8 0 291 289 424 734.8 0 424 290 183 734.8 0 183 291 106 734.8 0 106 292 86 734.8 0 86 293 84 734.8 0 84 294 65 734.8 0 65 295 83 734.8 0 83 296 63 734.8 0 63 297 83 734.8 0 83 298 144 734.8 0 144 299 105 734.8 0 105 300 132 734.8 0 132 301 132 734.8 0 132 Finished in 2.96 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 73,561 Reads with adapters: 5,598 (7.6%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 5,588 (7.6%) Total basepairs processed: 17,085,554 bp Total written (filtered): 1,534,712 bp (9.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5598 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 25 0.0 3 0 25 17 3247 0.0 3 3141 103 3 18 2271 0.0 3 2225 45 1 19 10 0.0 3 0 7 3 20 10 0.0 3 0 0 2 8 21 7 0.0 3 0 0 0 7 33 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 192 7 0.0 3 0 0 0 7 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.20 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 5,588 Reads with adapters: 709 (12.7%) Reads written (passing filters): 5,588 (100.0%) Total basepairs processed: 1,534,712 bp Total written (filtered): 1,532,580 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 709 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.2% C: 7.8% G: 37.8% T: 1.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 705 87.3 0 705 4 3 21.8 0 3 5 1 5.5 0 1