This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCTCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.56 s (25 us/read; 2.39 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 17,357 (0.6%) Reads that were too short: 4,035 (0.1%) Reads written (passing filters): 13,322 (0.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 2,662,819 bp (0.3%) === Adapter 1 === Sequence: TTCTCG; Type: regular 5'; Length: 6; Trimmed: 17357 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 93 734.8 0 93 7 31 734.8 0 31 8 61 734.8 0 61 9 56 734.8 0 56 10 35 734.8 0 35 11 21 734.8 0 21 12 23 734.8 0 23 13 28 734.8 0 28 14 24 734.8 0 24 15 25 734.8 0 25 16 14 734.8 0 14 17 22 734.8 0 22 18 23 734.8 0 23 19 34 734.8 0 34 20 19 734.8 0 19 21 19 734.8 0 19 22 24 734.8 0 24 23 27 734.8 0 27 24 21 734.8 0 21 25 22 734.8 0 22 26 28 734.8 0 28 27 40 734.8 0 40 28 22 734.8 0 22 29 17 734.8 0 17 30 24 734.8 0 24 31 25 734.8 0 25 32 32 734.8 0 32 33 32 734.8 0 32 34 28 734.8 0 28 35 30 734.8 0 30 36 27 734.8 0 27 37 32 734.8 0 32 38 25 734.8 0 25 39 22 734.8 0 22 40 35 734.8 0 35 41 16 734.8 0 16 42 26 734.8 0 26 43 25 734.8 0 25 44 769 734.8 0 769 45 59 734.8 0 59 46 28 734.8 0 28 47 33 734.8 0 33 48 24 734.8 0 24 49 21 734.8 0 21 50 26 734.8 0 26 51 14 734.8 0 14 52 40 734.8 0 40 53 32 734.8 0 32 54 30 734.8 0 30 55 26 734.8 0 26 56 73 734.8 0 73 57 37 734.8 0 37 58 50 734.8 0 50 59 64 734.8 0 64 60 34 734.8 0 34 61 34 734.8 0 34 62 110 734.8 0 110 63 20 734.8 0 20 64 30 734.8 0 30 65 27 734.8 0 27 66 19 734.8 0 19 67 18 734.8 0 18 68 33 734.8 0 33 69 34 734.8 0 34 70 34 734.8 0 34 71 38 734.8 0 38 72 22 734.8 0 22 73 29 734.8 0 29 74 31 734.8 0 31 75 47 734.8 0 47 76 47 734.8 0 47 77 70 734.8 0 70 78 28 734.8 0 28 79 36 734.8 0 36 80 42 734.8 0 42 81 23 734.8 0 23 82 45 734.8 0 45 83 42 734.8 0 42 84 46 734.8 0 46 85 54 734.8 0 54 86 32 734.8 0 32 87 37 734.8 0 37 88 51 734.8 0 51 89 71 734.8 0 71 90 1950 734.8 0 1950 91 1041 734.8 0 1041 92 346 734.8 0 346 93 30 734.8 0 30 94 53 734.8 0 53 95 30 734.8 0 30 96 35 734.8 0 35 97 41 734.8 0 41 98 34 734.8 0 34 99 26 734.8 0 26 100 34 734.8 0 34 101 45 734.8 0 45 102 38 734.8 0 38 103 44 734.8 0 44 104 35 734.8 0 35 105 30 734.8 0 30 106 93 734.8 0 93 107 72 734.8 0 72 108 148 734.8 0 148 109 52 734.8 0 52 110 85 734.8 0 85 111 1132 734.8 0 1132 112 749 734.8 0 749 113 111 734.8 0 111 114 31 734.8 0 31 115 48 734.8 0 48 116 33 734.8 0 33 117 29 734.8 0 29 118 37 734.8 0 37 119 12 734.8 0 12 120 21 734.8 0 21 121 18 734.8 0 18 122 18 734.8 0 18 123 24 734.8 0 24 124 31 734.8 0 31 125 32 734.8 0 32 126 28 734.8 0 28 127 24 734.8 0 24 128 13 734.8 0 13 129 35 734.8 0 35 130 43 734.8 0 43 131 41 734.8 0 41 132 27 734.8 0 27 133 49 734.8 0 49 134 29 734.8 0 29 135 37 734.8 0 37 136 38 734.8 0 38 137 37 734.8 0 37 138 19 734.8 0 19 139 27 734.8 0 27 140 32 734.8 0 32 141 26 734.8 0 26 142 35 734.8 0 35 143 84 734.8 0 84 144 41 734.8 0 41 145 48 734.8 0 48 146 31 734.8 0 31 147 30 734.8 0 30 148 24 734.8 0 24 149 45 734.8 0 45 150 31 734.8 0 31 151 23 734.8 0 23 152 42 734.8 0 42 153 33 734.8 0 33 154 33 734.8 0 33 155 35 734.8 0 35 156 39 734.8 0 39 157 41 734.8 0 41 158 34 734.8 0 34 159 39 734.8 0 39 160 36 734.8 0 36 161 146 734.8 0 146 162 130 734.8 0 130 163 110 734.8 0 110 164 147 734.8 0 147 165 44 734.8 0 44 166 19 734.8 0 19 167 30 734.8 0 30 168 31 734.8 0 31 169 41 734.8 0 41 170 32 734.8 0 32 171 48 734.8 0 48 172 59 734.8 0 59 173 34 734.8 0 34 174 17 734.8 0 17 175 29 734.8 0 29 176 30 734.8 0 30 177 108 734.8 0 108 178 63 734.8 0 63 179 41 734.8 0 41 180 26 734.8 0 26 181 33 734.8 0 33 182 24 734.8 0 24 183 20 734.8 0 20 184 23 734.8 0 23 185 27 734.8 0 27 186 29 734.8 0 29 187 23 734.8 0 23 188 30 734.8 0 30 189 66 734.8 0 66 190 63 734.8 0 63 191 51 734.8 0 51 192 34 734.8 0 34 193 18 734.8 0 18 194 24 734.8 0 24 195 27 734.8 0 27 196 33 734.8 0 33 197 49 734.8 0 49 198 18 734.8 0 18 199 37 734.8 0 37 200 45 734.8 0 45 201 28 734.8 0 28 202 26 734.8 0 26 203 22 734.8 0 22 204 34 734.8 0 34 205 36 734.8 0 36 206 21 734.8 0 21 207 30 734.8 0 30 208 25 734.8 0 25 209 36 734.8 0 36 210 29 734.8 0 29 211 76 734.8 0 76 212 54 734.8 0 54 213 50 734.8 0 50 214 35 734.8 0 35 215 43 734.8 0 43 216 39 734.8 0 39 217 43 734.8 0 43 218 33 734.8 0 33 219 34 734.8 0 34 220 24 734.8 0 24 221 32 734.8 0 32 222 27 734.8 0 27 223 35 734.8 0 35 224 113 734.8 0 113 225 117 734.8 0 117 226 66 734.8 0 66 227 36 734.8 0 36 228 39 734.8 0 39 229 50 734.8 0 50 230 32 734.8 0 32 231 31 734.8 0 31 232 40 734.8 0 40 233 32 734.8 0 32 234 45 734.8 0 45 235 29 734.8 0 29 236 77 734.8 0 77 237 48 734.8 0 48 238 59 734.8 0 59 239 43 734.8 0 43 240 27 734.8 0 27 241 31 734.8 0 31 242 55 734.8 0 55 243 71 734.8 0 71 244 153 734.8 0 153 245 168 734.8 0 168 246 103 734.8 0 103 247 61 734.8 0 61 248 36 734.8 0 36 249 18 734.8 0 18 250 25 734.8 0 25 251 28 734.8 0 28 252 32 734.8 0 32 253 20 734.8 0 20 254 38 734.8 0 38 255 52 734.8 0 52 256 34 734.8 0 34 257 32 734.8 0 32 258 36 734.8 0 36 259 44 734.8 0 44 260 35 734.8 0 35 261 26 734.8 0 26 262 25 734.8 0 25 263 33 734.8 0 33 264 38 734.8 0 38 265 54 734.8 0 54 266 29 734.8 0 29 267 25 734.8 0 25 268 29 734.8 0 29 269 20 734.8 0 20 270 32 734.8 0 32 271 35 734.8 0 35 272 28 734.8 0 28 273 20 734.8 0 20 274 26 734.8 0 26 275 19 734.8 0 19 276 30 734.8 0 30 277 36 734.8 0 36 278 27 734.8 0 27 279 27 734.8 0 27 280 24 734.8 0 24 281 25 734.8 0 25 282 31 734.8 0 31 283 26 734.8 0 26 284 36 734.8 0 36 285 38 734.8 0 38 286 53 734.8 0 53 287 36 734.8 0 36 288 34 734.8 0 34 289 20 734.8 0 20 290 42 734.8 0 42 291 37 734.8 0 37 292 21 734.8 0 21 293 37 734.8 0 37 294 45 734.8 0 45 295 20 734.8 0 20 296 41 734.8 0 41 297 53 734.8 0 53 298 43 734.8 0 43 299 32 734.8 0 32 300 24 734.8 0 24 301 18 734.8 0 18 Finished in 0.48 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 13,322 Reads with adapters: 19 (0.1%) Reads that were too short: 0 (0.0%) Reads written (passing filters): 19 (0.1%) Total basepairs processed: 2,662,819 bp Total written (filtered): 2,353 bp (0.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 20 1 0.0 3 1 52 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 10 0.0 3 0 0 0 10 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (526 us/read; 0.11 M reads/minute). === Summary === Total reads processed: 19 Reads with adapters: 1 (5.3%) Reads written (passing filters): 19 (100.0%) Total basepairs processed: 2,353 bp Total written (filtered): 2,350 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 0.3 0 1