This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.08 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 469,794 (15.6%) Reads that were too short: 338,142 (11.2%) Reads written (passing filters): 131,652 (4.4%) Total basepairs processed: 905,907,359 bp Total written (filtered): 31,142,291 bp (3.4%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 469794 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 636 734.8 0 636 7 62463 734.8 0 62463 8 159 734.8 0 159 9 3972 734.8 0 3972 10 73 734.8 0 73 11 71 734.8 0 71 12 60 734.8 0 60 13 60 734.8 0 60 14 67 734.8 0 67 15 61 734.8 0 61 16 58 734.8 0 58 17 62 734.8 0 62 18 60 734.8 0 60 19 56 734.8 0 56 20 59 734.8 0 59 21 68 734.8 0 68 22 60 734.8 0 60 23 51 734.8 0 51 24 76 734.8 0 76 25 65 734.8 0 65 26 54 734.8 0 54 27 56 734.8 0 56 28 87 734.8 0 87 29 81 734.8 0 81 30 149 734.8 0 149 31 611 734.8 0 611 32 59 734.8 0 59 33 75 734.8 0 75 34 80 734.8 0 80 35 80 734.8 0 80 36 106 734.8 0 106 37 107 734.8 0 107 38 98 734.8 0 98 39 50 734.8 0 50 40 55 734.8 0 55 41 41 734.8 0 41 42 42 734.8 0 42 43 57 734.8 0 57 44 61 734.8 0 61 45 56 734.8 0 56 46 60 734.8 0 60 47 44 734.8 0 44 48 40 734.8 0 40 49 37 734.8 0 37 50 33 734.8 0 33 51 43 734.8 0 43 52 44 734.8 0 44 53 48 734.8 0 48 54 49 734.8 0 49 55 58 734.8 0 58 56 2268 734.8 0 2268 57 339 734.8 0 339 58 46 734.8 0 46 59 48 734.8 0 48 60 67 734.8 0 67 61 50 734.8 0 50 62 37 734.8 0 37 63 49 734.8 0 49 64 50 734.8 0 50 65 73 734.8 0 73 66 86 734.8 0 86 67 57 734.8 0 57 68 68 734.8 0 68 69 100 734.8 0 100 70 115 734.8 0 115 71 109 734.8 0 109 72 161 734.8 0 161 73 241 734.8 0 241 74 242 734.8 0 242 75 602 734.8 0 602 76 464 734.8 0 464 77 729 734.8 0 729 78 823 734.8 0 823 79 308 734.8 0 308 80 546 734.8 0 546 81 551 734.8 0 551 82 409 734.8 0 409 83 207 734.8 0 207 84 311 734.8 0 311 85 277 734.8 0 277 86 232 734.8 0 232 87 226 734.8 0 226 88 426 734.8 0 426 89 306 734.8 0 306 90 470 734.8 0 470 91 253 734.8 0 253 92 355 734.8 0 355 93 438 734.8 0 438 94 412 734.8 0 412 95 341 734.8 0 341 96 869 734.8 0 869 97 850 734.8 0 850 98 1015 734.8 0 1015 99 964 734.8 0 964 100 552 734.8 0 552 101 385 734.8 0 385 102 211 734.8 0 211 103 149 734.8 0 149 104 124 734.8 0 124 105 291 734.8 0 291 106 999 734.8 0 999 107 704 734.8 0 704 108 659 734.8 0 659 109 439 734.8 0 439 110 669 734.8 0 669 111 627 734.8 0 627 112 601 734.8 0 601 113 483 734.8 0 483 114 468 734.8 0 468 115 298 734.8 0 298 116 208 734.8 0 208 117 204 734.8 0 204 118 242 734.8 0 242 119 694 734.8 0 694 120 643 734.8 0 643 121 445 734.8 0 445 122 283 734.8 0 283 123 641 734.8 0 641 124 509 734.8 0 509 125 355 734.8 0 355 126 346 734.8 0 346 127 320 734.8 0 320 128 515 734.8 0 515 129 463 734.8 0 463 130 347 734.8 0 347 131 326 734.8 0 326 132 655 734.8 0 655 133 377 734.8 0 377 134 442 734.8 0 442 135 328 734.8 0 328 136 285 734.8 0 285 137 255 734.8 0 255 138 371 734.8 0 371 139 1042 734.8 0 1042 140 872 734.8 0 872 141 505 734.8 0 505 142 241 734.8 0 241 143 242 734.8 0 242 144 241 734.8 0 241 145 269 734.8 0 269 146 395 734.8 0 395 147 278 734.8 0 278 148 305 734.8 0 305 149 277 734.8 0 277 150 252 734.8 0 252 151 176 734.8 0 176 152 212 734.8 0 212 153 224 734.8 0 224 154 275 734.8 0 275 155 327 734.8 0 327 156 300 734.8 0 300 157 311 734.8 0 311 158 335 734.8 0 335 159 2451 734.8 0 2451 160 4779 734.8 0 4779 161 2051 734.8 0 2051 162 229 734.8 0 229 163 258 734.8 0 258 164 246 734.8 0 246 165 326 734.8 0 326 166 248 734.8 0 248 167 276 734.8 0 276 168 300 734.8 0 300 169 186 734.8 0 186 170 149 734.8 0 149 171 147 734.8 0 147 172 202 734.8 0 202 173 292 734.8 0 292 174 375 734.8 0 375 175 224 734.8 0 224 176 254 734.8 0 254 177 194 734.8 0 194 178 220 734.8 0 220 179 221 734.8 0 221 180 313 734.8 0 313 181 247 734.8 0 247 182 184 734.8 0 184 183 301 734.8 0 301 184 363 734.8 0 363 185 371 734.8 0 371 186 237 734.8 0 237 187 225 734.8 0 225 188 246 734.8 0 246 189 272 734.8 0 272 190 254 734.8 0 254 191 250 734.8 0 250 192 316 734.8 0 316 193 310 734.8 0 310 194 342 734.8 0 342 195 472 734.8 0 472 196 396 734.8 0 396 197 218 734.8 0 218 198 202 734.8 0 202 199 213 734.8 0 213 200 448 734.8 0 448 201 564 734.8 0 564 202 359 734.8 0 359 203 447 734.8 0 447 204 463 734.8 0 463 205 403 734.8 0 403 206 595 734.8 0 595 207 978 734.8 0 978 208 755 734.8 0 755 209 702 734.8 0 702 210 530 734.8 0 530 211 504 734.8 0 504 212 750 734.8 0 750 213 580 734.8 0 580 214 351 734.8 0 351 215 269 734.8 0 269 216 205 734.8 0 205 217 212 734.8 0 212 218 487 734.8 0 487 219 384 734.8 0 384 220 458 734.8 0 458 221 585 734.8 0 585 222 404 734.8 0 404 223 518 734.8 0 518 224 510 734.8 0 510 225 341 734.8 0 341 226 179 734.8 0 179 227 318 734.8 0 318 228 549 734.8 0 549 229 370 734.8 0 370 230 436 734.8 0 436 231 509 734.8 0 509 232 4652 734.8 0 4652 233 2883 734.8 0 2883 234 1031 734.8 0 1031 235 315 734.8 0 315 236 328 734.8 0 328 237 544 734.8 0 544 238 867 734.8 0 867 239 302 734.8 0 302 240 799 734.8 0 799 241 728 734.8 0 728 242 700 734.8 0 700 243 884 734.8 0 884 244 476 734.8 0 476 245 376 734.8 0 376 246 193 734.8 0 193 247 293 734.8 0 293 248 550 734.8 0 550 249 1005 734.8 0 1005 250 1568 734.8 0 1568 251 1871 734.8 0 1871 252 2292 734.8 0 2292 253 5229 734.8 0 5229 254 10704 734.8 0 10704 255 131030 734.8 0 131030 256 112138 734.8 0 112138 257 24150 734.8 0 24150 258 544 734.8 0 544 259 356 734.8 0 356 260 582 734.8 0 582 261 1101 734.8 0 1101 262 2044 734.8 0 2044 263 1099 734.8 0 1099 264 282 734.8 0 282 265 239 734.8 0 239 266 261 734.8 0 261 267 185 734.8 0 185 268 160 734.8 0 160 269 320 734.8 0 320 270 302 734.8 0 302 271 383 734.8 0 383 272 382 734.8 0 382 273 247 734.8 0 247 274 140 734.8 0 140 275 232 734.8 0 232 276 292 734.8 0 292 277 225 734.8 0 225 278 160 734.8 0 160 279 227 734.8 0 227 280 225 734.8 0 225 281 225 734.8 0 225 282 498 734.8 0 498 283 633 734.8 0 633 284 415 734.8 0 415 285 127 734.8 0 127 286 100 734.8 0 100 287 159 734.8 0 159 288 239 734.8 0 239 289 332 734.8 0 332 290 337 734.8 0 337 291 232 734.8 0 232 292 208 734.8 0 208 293 209 734.8 0 209 294 199 734.8 0 199 295 328 734.8 0 328 296 498 734.8 0 498 297 463 734.8 0 463 298 869 734.8 0 869 299 952 734.8 0 952 300 458 734.8 0 458 301 614 734.8 0 614 Finished in 5.71 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 131,652 Reads with adapters: 62,964 (47.8%) Reads that were too short: 27 (0.0%) Reads written (passing filters): 62,937 (47.8%) Total basepairs processed: 31,142,291 bp Total written (filtered): 17,231,037 bp (55.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 62964 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 22 0.0 3 0 0 18 3 1 19 413 0.0 3 0 394 8 3 8 20 62128 0.0 4 61082 912 85 35 14 21 169 0.0 4 14 147 5 3 22 21 0.0 4 2 0 13 3 3 23 40 0.0 4 0 0 0 36 4 24 3 0.0 4 1 1 0 0 1 28 6 0.0 4 0 0 0 3 3 29 2 0.0 4 0 0 0 0 2 34 1 0.0 4 1 43 1 0.0 4 1 48 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 55 2 0.0 4 0 0 0 0 2 58 2 0.0 4 0 0 0 1 1 59 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 1 96 1 0.0 4 0 0 0 0 1 97 3 0.0 4 0 0 0 0 3 98 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 0 2 101 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 4 0.0 4 0 0 0 0 4 110 1 0.0 4 0 0 0 1 111 91 0.0 4 0 0 0 83 8 120 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 127 16 0.0 4 0 0 0 0 16 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 247 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.19 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 62,937 Reads with adapters: 6,169 (9.8%) Reads written (passing filters): 62,937 (100.0%) Total basepairs processed: 17,231,037 bp Total written (filtered): 16,951,267 bp (98.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6169 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.1% C: 7.6% G: 80.8% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 983.4 0 12 4 1 245.8 0 1 9 4 0.2 0 0 4 10 8 0.1 1 1 7 11 5197 0.0 1 33 5164 12 106 0.0 1 23 83 13 1 0.0 1 0 1 15 1 0.0 1 0 1 152 6 0.0 1 2 4 191 1 0.0 1 0 1 194 1 0.0 1 1 210 3 0.0 1 2 1 238 44 0.0 1 43 1 239 2 0.0 1 2 241 1 0.0 1 1 243 1 0.0 1 1 244 1 0.0 1 1 245 2 0.0 1 2 256 1 0.0 1 1 260 3 0.0 1 3 261 301 0.0 1 284 17 262 5 0.0 1 5 266 1 0.0 1 1 269 2 0.0 1 2 270 404 0.0 1 393 11 271 59 0.0 1 59 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.