This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCTAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 78.33 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 183,207 (6.1%) Reads that were too short: 46,694 (1.6%) Reads written (passing filters): 136,513 (4.5%) Total basepairs processed: 905,907,359 bp Total written (filtered): 33,079,090 bp (3.7%) === Adapter 1 === Sequence: GGCTAC; Type: regular 5'; Length: 6; Trimmed: 183207 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 495 734.8 0 495 7 74923 734.8 0 74923 8 122 734.8 0 122 9 17 734.8 0 17 10 15 734.8 0 15 11 18 734.8 0 18 12 26 734.8 0 26 13 278 734.8 0 278 14 385 734.8 0 385 15 160 734.8 0 160 16 14 734.8 0 14 17 10 734.8 0 10 18 13 734.8 0 13 19 21 734.8 0 21 20 11 734.8 0 11 21 13 734.8 0 13 22 7 734.8 0 7 23 9 734.8 0 9 24 8 734.8 0 8 25 4 734.8 0 4 26 15 734.8 0 15 27 15 734.8 0 15 28 21 734.8 0 21 29 550 734.8 0 550 30 29 734.8 0 29 31 16 734.8 0 16 32 37 734.8 0 37 33 11 734.8 0 11 34 11 734.8 0 11 35 25 734.8 0 25 36 28 734.8 0 28 37 29 734.8 0 29 38 404 734.8 0 404 39 47 734.8 0 47 40 40 734.8 0 40 41 18 734.8 0 18 42 3 734.8 0 3 43 10 734.8 0 10 44 16 734.8 0 16 45 10 734.8 0 10 46 6 734.8 0 6 47 5 734.8 0 5 48 4 734.8 0 4 49 20 734.8 0 20 50 8 734.8 0 8 51 13 734.8 0 13 52 6 734.8 0 6 53 15 734.8 0 15 54 14 734.8 0 14 55 14 734.8 0 14 56 11 734.8 0 11 57 10 734.8 0 10 58 6 734.8 0 6 59 14 734.8 0 14 60 7 734.8 0 7 61 11 734.8 0 11 62 9 734.8 0 9 63 10 734.8 0 10 64 10 734.8 0 10 65 7 734.8 0 7 66 7 734.8 0 7 67 16 734.8 0 16 68 13 734.8 0 13 69 6 734.8 0 6 70 5 734.8 0 5 71 7 734.8 0 7 72 10 734.8 0 10 73 5 734.8 0 5 74 8 734.8 0 8 75 7 734.8 0 7 76 12 734.8 0 12 77 6 734.8 0 6 78 5 734.8 0 5 79 13 734.8 0 13 80 8 734.8 0 8 81 12 734.8 0 12 82 16 734.8 0 16 83 73 734.8 0 73 84 67 734.8 0 67 85 38 734.8 0 38 86 15 734.8 0 15 87 30 734.8 0 30 88 31 734.8 0 31 89 9 734.8 0 9 90 6 734.8 0 6 91 10 734.8 0 10 92 8 734.8 0 8 93 8 734.8 0 8 94 7 734.8 0 7 95 8 734.8 0 8 96 16 734.8 0 16 97 13 734.8 0 13 98 11 734.8 0 11 99 10 734.8 0 10 100 34 734.8 0 34 101 22 734.8 0 22 102 35 734.8 0 35 103 17 734.8 0 17 104 33 734.8 0 33 105 23 734.8 0 23 106 33 734.8 0 33 107 26 734.8 0 26 108 40 734.8 0 40 109 50 734.8 0 50 110 103 734.8 0 103 111 268 734.8 0 268 112 60 734.8 0 60 113 118 734.8 0 118 114 182 734.8 0 182 115 127 734.8 0 127 116 142 734.8 0 142 117 211 734.8 0 211 118 154 734.8 0 154 119 267 734.8 0 267 120 241 734.8 0 241 121 312 734.8 0 312 122 406 734.8 0 406 123 832 734.8 0 832 124 19338 734.8 0 19338 125 27187 734.8 0 27187 126 3223 734.8 0 3223 127 70 734.8 0 70 128 29 734.8 0 29 129 41 734.8 0 41 130 55 734.8 0 55 131 26 734.8 0 26 132 18 734.8 0 18 133 36 734.8 0 36 134 13 734.8 0 13 135 15 734.8 0 15 136 13 734.8 0 13 137 18 734.8 0 18 138 16 734.8 0 16 139 13 734.8 0 13 140 25 734.8 0 25 141 24 734.8 0 24 142 21 734.8 0 21 143 10 734.8 0 10 144 18 734.8 0 18 145 34 734.8 0 34 146 18 734.8 0 18 147 126 734.8 0 126 148 79 734.8 0 79 149 35 734.8 0 35 150 17 734.8 0 17 151 17 734.8 0 17 152 129 734.8 0 129 153 268 734.8 0 268 154 47 734.8 0 47 155 43 734.8 0 43 156 32 734.8 0 32 157 179 734.8 0 179 158 212 734.8 0 212 159 248 734.8 0 248 160 154 734.8 0 154 161 102 734.8 0 102 162 69 734.8 0 69 163 17 734.8 0 17 164 21 734.8 0 21 165 15 734.8 0 15 166 13 734.8 0 13 167 24 734.8 0 24 168 30 734.8 0 30 169 30 734.8 0 30 170 24 734.8 0 24 171 14 734.8 0 14 172 49 734.8 0 49 173 64 734.8 0 64 174 44 734.8 0 44 175 25 734.8 0 25 176 72 734.8 0 72 177 587 734.8 0 587 178 491 734.8 0 491 179 241 734.8 0 241 180 42 734.8 0 42 181 15 734.8 0 15 182 16 734.8 0 16 183 22 734.8 0 22 184 12 734.8 0 12 185 14 734.8 0 14 186 16 734.8 0 16 187 18 734.8 0 18 188 16 734.8 0 16 189 9 734.8 0 9 190 25 734.8 0 25 191 19 734.8 0 19 192 21 734.8 0 21 193 14 734.8 0 14 194 21 734.8 0 21 195 15 734.8 0 15 196 19 734.8 0 19 197 14 734.8 0 14 198 16 734.8 0 16 199 17 734.8 0 17 200 25 734.8 0 25 201 12 734.8 0 12 202 12 734.8 0 12 203 15 734.8 0 15 204 15 734.8 0 15 205 12 734.8 0 12 206 7 734.8 0 7 207 26 734.8 0 26 208 62 734.8 0 62 209 38 734.8 0 38 210 12 734.8 0 12 211 9 734.8 0 9 212 14 734.8 0 14 213 19 734.8 0 19 214 29 734.8 0 29 215 13 734.8 0 13 216 14 734.8 0 14 217 18 734.8 0 18 218 9 734.8 0 9 219 18 734.8 0 18 220 16 734.8 0 16 221 17 734.8 0 17 222 19 734.8 0 19 223 13 734.8 0 13 224 13 734.8 0 13 225 19 734.8 0 19 226 10 734.8 0 10 227 12 734.8 0 12 228 17 734.8 0 17 229 29 734.8 0 29 230 26 734.8 0 26 231 24 734.8 0 24 232 17 734.8 0 17 233 89 734.8 0 89 234 142 734.8 0 142 235 70 734.8 0 70 236 30 734.8 0 30 237 10 734.8 0 10 238 16 734.8 0 16 239 19 734.8 0 19 240 20 734.8 0 20 241 22 734.8 0 22 242 11 734.8 0 11 243 14 734.8 0 14 244 21 734.8 0 21 245 5 734.8 0 5 246 19 734.8 0 19 247 15 734.8 0 15 248 18 734.8 0 18 249 19 734.8 0 19 250 13 734.8 0 13 251 10 734.8 0 10 252 16 734.8 0 16 253 16 734.8 0 16 254 17 734.8 0 17 255 26 734.8 0 26 256 54 734.8 0 54 257 442 734.8 0 442 258 1263 734.8 0 1263 259 192 734.8 0 192 260 53 734.8 0 53 261 132 734.8 0 132 262 50 734.8 0 50 263 174 734.8 0 174 264 145 734.8 0 145 265 71 734.8 0 71 266 14 734.8 0 14 267 34 734.8 0 34 268 79 734.8 0 79 269 124 734.8 0 124 270 30 734.8 0 30 271 16 734.8 0 16 272 18 734.8 0 18 273 11 734.8 0 11 274 23 734.8 0 23 275 17 734.8 0 17 276 16 734.8 0 16 277 15 734.8 0 15 278 20 734.8 0 20 279 9 734.8 0 9 280 47 734.8 0 47 281 22 734.8 0 22 282 25 734.8 0 25 283 18 734.8 0 18 284 25 734.8 0 25 285 16 734.8 0 16 286 20 734.8 0 20 287 80 734.8 0 80 288 400 734.8 0 400 289 306 734.8 0 306 290 139 734.8 0 139 291 56 734.8 0 56 292 175 734.8 0 175 293 12055 734.8 0 12055 294 21592 734.8 0 21592 295 6927 734.8 0 6927 296 451 734.8 0 451 297 28 734.8 0 28 298 24 734.8 0 24 299 36 734.8 0 36 300 20 734.8 0 20 301 18 734.8 0 18 Finished in 5.54 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 136,513 Reads with adapters: 75,133 (55.0%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 75,119 (55.0%) Total basepairs processed: 33,079,090 bp Total written (filtered): 20,805,393 bp (62.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 75133 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 28 0.0 3 0 0 28 16 569 0.0 3 0 561 7 1 17 74253 0.0 3 73332 909 11 1 18 132 0.0 3 17 107 6 2 19 44 0.0 3 0 0 41 3 20 68 0.0 3 0 0 0 68 28 2 0.0 3 0 0 0 2 29 1 0.0 3 1 31 2 0.0 3 0 0 0 2 35 1 0.0 3 1 40 4 0.0 3 0 0 0 4 58 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 68 8 0.0 3 0 0 0 8 71 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 129 4 0.0 3 0 0 0 4 140 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.72 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 75,119 Reads with adapters: 10,028 (13.3%) Reads written (passing filters): 75,119 (100.0%) Total basepairs processed: 20,805,393 bp Total written (filtered): 20,775,283 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 10028 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.5% C: 85.8% G: 2.2% T: 0.5% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "C" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 10010 1173.7 0 10010 4 14 293.4 0 14 5 2 73.4 0 2 7 2 4.6 0 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.