This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.58 s (25 us/read; 2.36 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 86,633 (2.9%) Reads that were too short: 25,500 (0.8%) Reads written (passing filters): 61,133 (2.0%) Total basepairs processed: 905,907,359 bp Total written (filtered): 15,063,534 bp (1.7%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 86633 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1095 734.8 0 1095 7 548 734.8 0 548 8 35948 734.8 0 35948 9 2695 734.8 0 2695 10 24 734.8 0 24 11 29 734.8 0 29 12 25 734.8 0 25 13 22 734.8 0 22 14 13 734.8 0 13 15 20 734.8 0 20 16 22 734.8 0 22 17 24 734.8 0 24 18 26 734.8 0 26 19 25 734.8 0 25 20 32 734.8 0 32 21 108 734.8 0 108 22 39 734.8 0 39 23 29 734.8 0 29 24 27 734.8 0 27 25 30 734.8 0 30 26 331 734.8 0 331 27 91 734.8 0 91 28 42 734.8 0 42 29 54 734.8 0 54 30 17 734.8 0 17 31 12 734.8 0 12 32 26 734.8 0 26 33 67 734.8 0 67 34 55 734.8 0 55 35 23 734.8 0 23 36 28 734.8 0 28 37 29 734.8 0 29 38 39 734.8 0 39 39 31 734.8 0 31 40 19 734.8 0 19 41 28 734.8 0 28 42 28 734.8 0 28 43 23 734.8 0 23 44 33 734.8 0 33 45 32 734.8 0 32 46 17 734.8 0 17 47 35 734.8 0 35 48 26 734.8 0 26 49 10 734.8 0 10 50 23 734.8 0 23 51 22 734.8 0 22 52 15 734.8 0 15 53 33 734.8 0 33 54 20 734.8 0 20 55 31 734.8 0 31 56 24 734.8 0 24 57 18 734.8 0 18 58 27 734.8 0 27 59 31 734.8 0 31 60 29 734.8 0 29 61 34 734.8 0 34 62 32 734.8 0 32 63 16 734.8 0 16 64 45 734.8 0 45 65 46 734.8 0 46 66 22 734.8 0 22 67 30 734.8 0 30 68 35 734.8 0 35 69 36 734.8 0 36 70 47 734.8 0 47 71 52 734.8 0 52 72 54 734.8 0 54 73 26 734.8 0 26 74 30 734.8 0 30 75 41 734.8 0 41 76 29 734.8 0 29 77 37 734.8 0 37 78 36 734.8 0 36 79 187 734.8 0 187 80 269 734.8 0 269 81 103 734.8 0 103 82 70 734.8 0 70 83 84 734.8 0 84 84 113 734.8 0 113 85 67 734.8 0 67 86 101 734.8 0 101 87 129 734.8 0 129 88 95 734.8 0 95 89 72 734.8 0 72 90 82 734.8 0 82 91 42 734.8 0 42 92 42 734.8 0 42 93 64 734.8 0 64 94 45 734.8 0 45 95 79 734.8 0 79 96 146 734.8 0 146 97 124 734.8 0 124 98 71 734.8 0 71 99 41 734.8 0 41 100 70 734.8 0 70 101 44 734.8 0 44 102 41 734.8 0 41 103 27 734.8 0 27 104 21 734.8 0 21 105 32 734.8 0 32 106 31 734.8 0 31 107 25 734.8 0 25 108 38 734.8 0 38 109 49 734.8 0 49 110 23 734.8 0 23 111 40 734.8 0 40 112 103 734.8 0 103 113 88 734.8 0 88 114 68 734.8 0 68 115 51 734.8 0 51 116 52 734.8 0 52 117 297 734.8 0 297 118 235 734.8 0 235 119 115 734.8 0 115 120 56 734.8 0 56 121 51 734.8 0 51 122 67 734.8 0 67 123 59 734.8 0 59 124 53 734.8 0 53 125 53 734.8 0 53 126 57 734.8 0 57 127 95 734.8 0 95 128 70 734.8 0 70 129 57 734.8 0 57 130 100 734.8 0 100 131 255 734.8 0 255 132 246 734.8 0 246 133 157 734.8 0 157 134 92 734.8 0 92 135 92 734.8 0 92 136 88 734.8 0 88 137 75 734.8 0 75 138 113 734.8 0 113 139 137 734.8 0 137 140 87 734.8 0 87 141 65 734.8 0 65 142 72 734.8 0 72 143 55 734.8 0 55 144 63 734.8 0 63 145 94 734.8 0 94 146 97 734.8 0 97 147 102 734.8 0 102 148 70 734.8 0 70 149 64 734.8 0 64 150 56 734.8 0 56 151 60 734.8 0 60 152 46 734.8 0 46 153 52 734.8 0 52 154 60 734.8 0 60 155 64 734.8 0 64 156 142 734.8 0 142 157 105 734.8 0 105 158 94 734.8 0 94 159 66 734.8 0 66 160 60 734.8 0 60 161 66 734.8 0 66 162 62 734.8 0 62 163 65 734.8 0 65 164 51 734.8 0 51 165 71 734.8 0 71 166 86 734.8 0 86 167 74 734.8 0 74 168 104 734.8 0 104 169 115 734.8 0 115 170 76 734.8 0 76 171 92 734.8 0 92 172 76 734.8 0 76 173 86 734.8 0 86 174 86 734.8 0 86 175 113 734.8 0 113 176 254 734.8 0 254 177 621 734.8 0 621 178 1305 734.8 0 1305 179 776 734.8 0 776 180 1394 734.8 0 1394 181 781 734.8 0 781 182 797 734.8 0 797 183 375 734.8 0 375 184 261 734.8 0 261 185 132 734.8 0 132 186 74 734.8 0 74 187 72 734.8 0 72 188 95 734.8 0 95 189 85 734.8 0 85 190 51 734.8 0 51 191 264 734.8 0 264 192 80 734.8 0 80 193 72 734.8 0 72 194 170 734.8 0 170 195 291 734.8 0 291 196 486 734.8 0 486 197 756 734.8 0 756 198 257 734.8 0 257 199 109 734.8 0 109 200 130 734.8 0 130 201 401 734.8 0 401 202 371 734.8 0 371 203 209 734.8 0 209 204 86 734.8 0 86 205 57 734.8 0 57 206 65 734.8 0 65 207 77 734.8 0 77 208 76 734.8 0 76 209 46 734.8 0 46 210 54 734.8 0 54 211 47 734.8 0 47 212 59 734.8 0 59 213 77 734.8 0 77 214 96 734.8 0 96 215 197 734.8 0 197 216 173 734.8 0 173 217 94 734.8 0 94 218 45 734.8 0 45 219 68 734.8 0 68 220 97 734.8 0 97 221 91 734.8 0 91 222 121 734.8 0 121 223 390 734.8 0 390 224 519 734.8 0 519 225 97 734.8 0 97 226 74 734.8 0 74 227 70 734.8 0 70 228 68 734.8 0 68 229 94 734.8 0 94 230 56 734.8 0 56 231 68 734.8 0 68 232 77 734.8 0 77 233 46 734.8 0 46 234 170 734.8 0 170 235 455 734.8 0 455 236 118 734.8 0 118 237 83 734.8 0 83 238 76 734.8 0 76 239 428 734.8 0 428 240 641 734.8 0 641 241 305 734.8 0 305 242 123 734.8 0 123 243 101 734.8 0 101 244 562 734.8 0 562 245 64 734.8 0 64 246 75 734.8 0 75 247 84 734.8 0 84 248 104 734.8 0 104 249 112 734.8 0 112 250 84 734.8 0 84 251 42 734.8 0 42 252 81 734.8 0 81 253 61 734.8 0 61 254 91 734.8 0 91 255 84 734.8 0 84 256 118 734.8 0 118 257 372 734.8 0 372 258 438 734.8 0 438 259 1614 734.8 0 1614 260 2141 734.8 0 2141 261 718 734.8 0 718 262 95 734.8 0 95 263 120 734.8 0 120 264 410 734.8 0 410 265 755 734.8 0 755 266 532 734.8 0 532 267 132 734.8 0 132 268 107 734.8 0 107 269 122 734.8 0 122 270 57 734.8 0 57 271 42 734.8 0 42 272 34 734.8 0 34 273 38 734.8 0 38 274 96 734.8 0 96 275 198 734.8 0 198 276 86 734.8 0 86 277 57 734.8 0 57 278 48 734.8 0 48 279 59 734.8 0 59 280 98 734.8 0 98 281 100 734.8 0 100 282 56 734.8 0 56 283 55 734.8 0 55 284 74 734.8 0 74 285 59 734.8 0 59 286 104 734.8 0 104 287 173 734.8 0 173 288 126 734.8 0 126 289 131 734.8 0 131 290 129 734.8 0 129 291 2566 734.8 0 2566 292 3302 734.8 0 3302 293 960 734.8 0 960 294 449 734.8 0 449 295 181 734.8 0 181 296 208 734.8 0 208 297 238 734.8 0 238 298 92 734.8 0 92 299 75 734.8 0 75 300 148 734.8 0 148 301 78 734.8 0 78 Finished in 2.89 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 61,133 Reads with adapters: 2,829 (4.6%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 2,812 (4.6%) Total basepairs processed: 15,063,534 bp Total written (filtered): 751,246 bp (5.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2829 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 1 0.0 3 0 0 1 19 26 0.0 3 0 23 2 1 20 2630 0.0 4 2476 134 15 4 1 21 16 0.0 4 4 11 0 0 1 22 1 0.0 4 0 0 1 23 2 0.0 4 1 0 0 0 1 26 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 49 3 0.0 4 3 63 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 107 7 0.0 4 0 0 0 0 7 111 28 0.0 4 0 0 0 23 5 112 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 85 0.0 4 0 0 0 0 85 128 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 153 3 0.0 4 0 0 0 0 3 210 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 233 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 2,812 Reads with adapters: 368 (13.1%) Reads written (passing filters): 2,812 (100.0%) Total basepairs processed: 751,246 bp Total written (filtered): 699,284 bp (93.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 368 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 58.2% C: 2.4% G: 38.6% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 9 67 0.0 0 6 61 10 66 0.0 1 3 63 11 29 0.0 1 1 28 12 1 0.0 1 0 1 150 2 0.0 1 2 199 9 0.0 1 7 2 208 1 0.0 1 1 228 49 0.0 1 45 4 229 1 0.0 1 1 230 3 0.0 1 3 232 1 0.0 1 1 252 1 0.0 1 1 257 126 0.0 1 119 7 258 8 0.0 1 8 259 1 0.0 1 1 260 2 0.0 1 2 269 1 0.0 1 1