This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 79.02 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 102,291 (3.4%) Reads that were too short: 50,229 (1.7%) Reads written (passing filters): 52,062 (1.7%) Total basepairs processed: 905,907,359 bp Total written (filtered): 14,235,064 bp (1.6%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 102291 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3235 734.8 0 3235 7 29217 734.8 0 29217 8 7240 734.8 0 7240 9 27 734.8 0 27 10 51 734.8 0 51 11 13 734.8 0 13 12 22 734.8 0 22 13 18 734.8 0 18 14 19 734.8 0 19 15 12 734.8 0 12 16 27 734.8 0 27 17 20 734.8 0 20 18 22 734.8 0 22 19 22 734.8 0 22 20 70 734.8 0 70 21 29 734.8 0 29 22 13 734.8 0 13 23 16 734.8 0 16 24 18 734.8 0 18 25 20 734.8 0 20 26 16 734.8 0 16 27 20 734.8 0 20 28 15 734.8 0 15 29 24 734.8 0 24 30 16 734.8 0 16 31 16 734.8 0 16 32 14 734.8 0 14 33 17 734.8 0 17 34 18 734.8 0 18 35 23 734.8 0 23 36 15 734.8 0 15 37 9 734.8 0 9 38 17 734.8 0 17 39 16 734.8 0 16 40 15 734.8 0 15 41 13 734.8 0 13 42 17 734.8 0 17 43 12 734.8 0 12 44 18 734.8 0 18 45 12 734.8 0 12 46 25 734.8 0 25 47 41 734.8 0 41 48 15 734.8 0 15 49 13 734.8 0 13 50 24 734.8 0 24 51 34 734.8 0 34 52 18 734.8 0 18 53 17 734.8 0 17 54 17 734.8 0 17 55 33 734.8 0 33 56 11 734.8 0 11 57 19 734.8 0 19 58 17 734.8 0 17 59 31 734.8 0 31 60 20 734.8 0 20 61 34 734.8 0 34 62 40 734.8 0 40 63 27 734.8 0 27 64 56 734.8 0 56 65 56 734.8 0 56 66 22 734.8 0 22 67 21 734.8 0 21 68 36 734.8 0 36 69 45 734.8 0 45 70 45 734.8 0 45 71 49 734.8 0 49 72 69 734.8 0 69 73 61 734.8 0 61 74 118 734.8 0 118 75 3530 734.8 0 3530 76 2823 734.8 0 2823 77 989 734.8 0 989 78 19 734.8 0 19 79 15 734.8 0 15 80 17 734.8 0 17 81 8 734.8 0 8 82 9 734.8 0 9 83 13 734.8 0 13 84 23 734.8 0 23 85 16 734.8 0 16 86 10 734.8 0 10 87 17 734.8 0 17 88 28 734.8 0 28 89 18 734.8 0 18 90 15 734.8 0 15 91 27 734.8 0 27 92 12 734.8 0 12 93 9 734.8 0 9 94 12 734.8 0 12 95 22 734.8 0 22 96 11 734.8 0 11 97 8 734.8 0 8 98 15 734.8 0 15 99 7 734.8 0 7 100 2 734.8 0 2 101 7 734.8 0 7 102 10 734.8 0 10 103 11 734.8 0 11 104 20 734.8 0 20 105 16 734.8 0 16 106 9 734.8 0 9 107 4 734.8 0 4 108 9 734.8 0 9 109 9 734.8 0 9 110 5 734.8 0 5 111 11 734.8 0 11 112 13 734.8 0 13 113 14 734.8 0 14 114 12 734.8 0 12 115 13 734.8 0 13 116 16 734.8 0 16 117 16 734.8 0 16 118 7 734.8 0 7 119 17 734.8 0 17 120 15 734.8 0 15 121 7 734.8 0 7 122 12 734.8 0 12 123 16 734.8 0 16 124 11 734.8 0 11 125 225 734.8 0 225 126 20 734.8 0 20 127 17 734.8 0 17 128 32 734.8 0 32 129 18 734.8 0 18 130 21 734.8 0 21 131 9 734.8 0 9 132 41 734.8 0 41 133 25 734.8 0 25 134 35 734.8 0 35 135 27 734.8 0 27 136 45 734.8 0 45 137 32 734.8 0 32 138 16 734.8 0 16 139 5 734.8 0 5 140 10 734.8 0 10 141 14 734.8 0 14 142 28 734.8 0 28 143 19 734.8 0 19 144 5 734.8 0 5 145 9 734.8 0 9 146 9 734.8 0 9 147 11 734.8 0 11 148 8 734.8 0 8 149 14 734.8 0 14 150 9 734.8 0 9 151 5 734.8 0 5 152 6 734.8 0 6 153 7 734.8 0 7 154 17 734.8 0 17 155 25 734.8 0 25 156 14 734.8 0 14 157 14 734.8 0 14 158 13 734.8 0 13 159 21 734.8 0 21 160 13 734.8 0 13 161 50 734.8 0 50 162 26 734.8 0 26 163 12 734.8 0 12 164 22 734.8 0 22 165 18 734.8 0 18 166 51 734.8 0 51 167 60 734.8 0 60 168 62 734.8 0 62 169 31 734.8 0 31 170 19 734.8 0 19 171 8 734.8 0 8 172 12 734.8 0 12 173 10 734.8 0 10 174 6 734.8 0 6 175 13 734.8 0 13 176 22 734.8 0 22 177 14 734.8 0 14 178 14 734.8 0 14 179 16 734.8 0 16 180 26 734.8 0 26 181 17 734.8 0 17 182 20 734.8 0 20 183 37 734.8 0 37 184 23 734.8 0 23 185 16 734.8 0 16 186 44 734.8 0 44 187 38 734.8 0 38 188 37 734.8 0 37 189 25 734.8 0 25 190 12 734.8 0 12 191 27 734.8 0 27 192 46 734.8 0 46 193 190 734.8 0 190 194 165 734.8 0 165 195 77 734.8 0 77 196 40 734.8 0 40 197 190 734.8 0 190 198 171 734.8 0 171 199 94 734.8 0 94 200 38 734.8 0 38 201 21 734.8 0 21 202 38 734.8 0 38 203 104 734.8 0 104 204 118 734.8 0 118 205 62 734.8 0 62 206 27 734.8 0 27 207 12 734.8 0 12 208 17 734.8 0 17 209 20 734.8 0 20 210 24 734.8 0 24 211 34 734.8 0 34 212 14 734.8 0 14 213 53 734.8 0 53 214 76 734.8 0 76 215 39 734.8 0 39 216 20 734.8 0 20 217 70 734.8 0 70 218 97 734.8 0 97 219 82 734.8 0 82 220 32 734.8 0 32 221 49 734.8 0 49 222 29 734.8 0 29 223 278 734.8 0 278 224 201 734.8 0 201 225 158 734.8 0 158 226 67 734.8 0 67 227 88 734.8 0 88 228 61 734.8 0 61 229 26 734.8 0 26 230 17 734.8 0 17 231 52 734.8 0 52 232 57 734.8 0 57 233 110 734.8 0 110 234 136 734.8 0 136 235 247 734.8 0 247 236 310 734.8 0 310 237 340 734.8 0 340 238 4295 734.8 0 4295 239 12108 734.8 0 12108 240 1398 734.8 0 1398 241 165 734.8 0 165 242 102 734.8 0 102 243 141 734.8 0 141 244 198 734.8 0 198 245 167 734.8 0 167 246 252 734.8 0 252 247 319 734.8 0 319 248 2172 734.8 0 2172 249 7523 734.8 0 7523 250 11216 734.8 0 11216 251 906 734.8 0 906 252 50 734.8 0 50 253 46 734.8 0 46 254 47 734.8 0 47 255 34 734.8 0 34 256 22 734.8 0 22 257 14 734.8 0 14 258 9 734.8 0 9 259 26 734.8 0 26 260 61 734.8 0 61 261 15 734.8 0 15 262 25 734.8 0 25 263 11 734.8 0 11 264 31 734.8 0 31 265 22 734.8 0 22 266 30 734.8 0 30 267 14 734.8 0 14 268 15 734.8 0 15 269 22 734.8 0 22 270 17 734.8 0 17 271 19 734.8 0 19 272 35 734.8 0 35 273 11 734.8 0 11 274 20 734.8 0 20 275 29 734.8 0 29 276 18 734.8 0 18 277 35 734.8 0 35 278 138 734.8 0 138 279 666 734.8 0 666 280 348 734.8 0 348 281 120 734.8 0 120 282 1087 734.8 0 1087 283 1966 734.8 0 1966 284 458 734.8 0 458 285 36 734.8 0 36 286 43 734.8 0 43 287 69 734.8 0 69 288 17 734.8 0 17 289 9 734.8 0 9 290 9 734.8 0 9 291 10 734.8 0 10 292 15 734.8 0 15 293 14 734.8 0 14 294 96 734.8 0 96 295 108 734.8 0 108 296 78 734.8 0 78 297 38 734.8 0 38 298 13 734.8 0 13 299 29 734.8 0 29 300 13 734.8 0 13 301 44 734.8 0 44 Finished in 2.69 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 52,062 Reads with adapters: 10,233 (19.7%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 10,219 (19.6%) Total basepairs processed: 14,235,064 bp Total written (filtered): 2,767,065 bp (19.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10233 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 18 7 0.0 3 0 0 4 2 1 19 113 0.0 3 0 103 6 2 2 20 7203 0.0 4 6966 194 31 8 4 21 64 0.0 4 5 56 2 0 1 22 2587 0.0 4 2502 73 7 4 1 23 19 0.0 4 1 4 0 11 3 24 1 0.0 4 1 25 3 0.0 4 0 0 0 3 27 1 0.0 4 1 40 1 0.0 4 1 43 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 59 22 0.0 4 0 0 0 0 22 83 1 0.0 4 0 0 0 0 1 85 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 1 111 23 0.0 4 0 0 0 23 126 3 0.0 4 0 0 0 0 3 127 166 0.0 4 0 0 0 0 166 145 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.36 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 10,219 Reads with adapters: 1,311 (12.8%) Reads written (passing filters): 10,219 (100.0%) Total basepairs processed: 2,767,065 bp Total written (filtered): 2,646,100 bp (95.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1311 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 33.6% C: 3.2% G: 62.9% T: 0.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 159.7 0 1 9 1 0.0 0 0 1 10 154 0.0 1 7 147 11 723 0.0 1 1 722 12 1 0.0 1 0 1 19 1 0.0 1 0 1 209 1 0.0 1 0 1 239 1 0.0 1 1 258 4 0.0 1 4 259 363 0.0 1 350 13 260 21 0.0 1 19 2 261 15 0.0 1 15 262 22 0.0 1 22 263 1 0.0 1 1 265 2 0.0 1 2