This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGATCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 82.11 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 577,609 (19.2%) Reads that were too short: 4,566 (0.2%) Reads written (passing filters): 573,043 (19.0%) Total basepairs processed: 905,907,359 bp Total written (filtered): 146,260,403 bp (16.1%) === Adapter 1 === Sequence: TGATCC; Type: regular 5'; Length: 6; Trimmed: 577609 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1118 734.8 0 1118 7 6795 734.8 0 6795 8 38 734.8 0 38 9 160 734.8 0 160 10 39 734.8 0 39 11 62 734.8 0 62 12 459 734.8 0 459 13 24 734.8 0 24 14 19 734.8 0 19 15 22 734.8 0 22 16 20 734.8 0 20 17 35 734.8 0 35 18 32 734.8 0 32 19 79 734.8 0 79 20 76 734.8 0 76 21 207 734.8 0 207 22 762 734.8 0 762 23 102 734.8 0 102 24 288 734.8 0 288 25 262 734.8 0 262 26 184 734.8 0 184 27 687 734.8 0 687 28 271 734.8 0 271 29 332 734.8 0 332 30 141 734.8 0 141 31 205 734.8 0 205 32 416 734.8 0 416 33 624 734.8 0 624 34 1136 734.8 0 1136 35 1761 734.8 0 1761 36 2949 734.8 0 2949 37 5321 734.8 0 5321 38 7400 734.8 0 7400 39 184629 734.8 0 184629 40 150457 734.8 0 150457 41 38607 734.8 0 38607 42 320 734.8 0 320 43 152 734.8 0 152 44 149 734.8 0 149 45 309 734.8 0 309 46 802 734.8 0 802 47 169 734.8 0 169 48 338 734.8 0 338 49 516 734.8 0 516 50 354 734.8 0 354 51 356 734.8 0 356 52 618 734.8 0 618 53 513 734.8 0 513 54 720 734.8 0 720 55 634 734.8 0 634 56 964 734.8 0 964 57 1050 734.8 0 1050 58 2260 734.8 0 2260 59 59099 734.8 0 59099 60 78021 734.8 0 78021 61 12883 734.8 0 12883 62 146 734.8 0 146 63 18 734.8 0 18 64 9 734.8 0 9 65 6 734.8 0 6 66 3 734.8 0 3 67 6 734.8 0 6 68 7 734.8 0 7 69 6 734.8 0 6 70 10 734.8 0 10 71 4 734.8 0 4 72 59 734.8 0 59 73 36 734.8 0 36 74 90 734.8 0 90 75 7 734.8 0 7 76 5 734.8 0 5 77 4 734.8 0 4 78 1 734.8 0 1 79 4 734.8 0 4 80 6 734.8 0 6 81 19 734.8 0 19 82 63 734.8 0 63 83 11 734.8 0 11 84 7 734.8 0 7 85 5 734.8 0 5 86 4 734.8 0 4 87 2 734.8 0 2 88 7 734.8 0 7 89 14 734.8 0 14 90 7 734.8 0 7 91 6 734.8 0 6 92 4 734.8 0 4 93 6 734.8 0 6 94 6 734.8 0 6 95 11 734.8 0 11 96 15 734.8 0 15 97 12 734.8 0 12 98 39 734.8 0 39 99 66 734.8 0 66 100 16 734.8 0 16 101 298 734.8 0 298 102 448 734.8 0 448 103 74 734.8 0 74 104 10 734.8 0 10 105 9 734.8 0 9 106 12 734.8 0 12 107 7 734.8 0 7 108 4 734.8 0 4 109 6 734.8 0 6 110 14 734.8 0 14 111 150 734.8 0 150 112 439 734.8 0 439 113 55 734.8 0 55 114 8 734.8 0 8 115 12 734.8 0 12 116 9 734.8 0 9 117 46 734.8 0 46 118 25 734.8 0 25 119 17 734.8 0 17 120 8 734.8 0 8 121 15 734.8 0 15 122 10 734.8 0 10 123 15 734.8 0 15 124 11 734.8 0 11 125 15 734.8 0 15 126 8 734.8 0 8 127 2 734.8 0 2 128 6 734.8 0 6 129 17 734.8 0 17 130 42 734.8 0 42 131 32 734.8 0 32 132 18 734.8 0 18 133 19 734.8 0 19 134 8 734.8 0 8 135 15 734.8 0 15 136 19 734.8 0 19 137 18 734.8 0 18 138 13 734.8 0 13 139 16 734.8 0 16 140 14 734.8 0 14 141 20 734.8 0 20 142 15 734.8 0 15 143 27 734.8 0 27 144 29 734.8 0 29 145 14 734.8 0 14 146 18 734.8 0 18 147 15 734.8 0 15 148 29 734.8 0 29 149 23 734.8 0 23 150 17 734.8 0 17 151 22 734.8 0 22 152 14 734.8 0 14 153 17 734.8 0 17 154 12 734.8 0 12 155 14 734.8 0 14 156 31 734.8 0 31 157 32 734.8 0 32 158 22 734.8 0 22 159 23 734.8 0 23 160 20 734.8 0 20 161 61 734.8 0 61 162 26 734.8 0 26 163 35 734.8 0 35 164 26 734.8 0 26 165 26 734.8 0 26 166 30 734.8 0 30 167 39 734.8 0 39 168 60 734.8 0 60 169 646 734.8 0 646 170 1028 734.8 0 1028 171 107 734.8 0 107 172 21 734.8 0 21 173 12 734.8 0 12 174 13 734.8 0 13 175 10 734.8 0 10 176 20 734.8 0 20 177 39 734.8 0 39 178 46 734.8 0 46 179 21 734.8 0 21 180 23 734.8 0 23 181 17 734.8 0 17 182 12 734.8 0 12 183 30 734.8 0 30 184 82 734.8 0 82 185 56 734.8 0 56 186 73 734.8 0 73 187 99 734.8 0 99 188 51 734.8 0 51 189 40 734.8 0 40 190 17 734.8 0 17 191 13 734.8 0 13 192 17 734.8 0 17 193 19 734.8 0 19 194 27 734.8 0 27 195 34 734.8 0 34 196 45 734.8 0 45 197 68 734.8 0 68 198 299 734.8 0 299 199 409 734.8 0 409 200 227 734.8 0 227 201 48 734.8 0 48 202 39 734.8 0 39 203 33 734.8 0 33 204 39 734.8 0 39 205 39 734.8 0 39 206 27 734.8 0 27 207 28 734.8 0 28 208 41 734.8 0 41 209 36 734.8 0 36 210 23 734.8 0 23 211 18 734.8 0 18 212 32 734.8 0 32 213 35 734.8 0 35 214 37 734.8 0 37 215 16 734.8 0 16 216 21 734.8 0 21 217 34 734.8 0 34 218 23 734.8 0 23 219 29 734.8 0 29 220 29 734.8 0 29 221 23 734.8 0 23 222 44 734.8 0 44 223 150 734.8 0 150 224 125 734.8 0 125 225 101 734.8 0 101 226 428 734.8 0 428 227 202 734.8 0 202 228 270 734.8 0 270 229 237 734.8 0 237 230 38 734.8 0 38 231 14 734.8 0 14 232 17 734.8 0 17 233 17 734.8 0 17 234 21 734.8 0 21 235 13 734.8 0 13 236 16 734.8 0 16 237 33 734.8 0 33 238 32 734.8 0 32 239 20 734.8 0 20 240 22 734.8 0 22 241 31 734.8 0 31 242 27 734.8 0 27 243 24 734.8 0 24 244 32 734.8 0 32 245 14 734.8 0 14 246 19 734.8 0 19 247 18 734.8 0 18 248 17 734.8 0 17 249 33 734.8 0 33 250 24 734.8 0 24 251 12 734.8 0 12 252 10 734.8 0 10 253 2 734.8 0 2 254 20 734.8 0 20 255 39 734.8 0 39 256 42 734.8 0 42 257 45 734.8 0 45 258 38 734.8 0 38 259 33 734.8 0 33 260 22 734.8 0 22 261 18 734.8 0 18 262 27 734.8 0 27 263 50 734.8 0 50 264 60 734.8 0 60 265 32 734.8 0 32 266 18 734.8 0 18 267 16 734.8 0 16 268 14 734.8 0 14 269 19 734.8 0 19 270 34 734.8 0 34 271 130 734.8 0 130 272 132 734.8 0 132 273 151 734.8 0 151 274 89 734.8 0 89 275 117 734.8 0 117 276 29 734.8 0 29 277 18 734.8 0 18 278 18 734.8 0 18 279 23 734.8 0 23 280 22 734.8 0 22 281 21 734.8 0 21 282 30 734.8 0 30 283 16 734.8 0 16 284 27 734.8 0 27 285 46 734.8 0 46 286 58 734.8 0 58 287 17 734.8 0 17 288 22 734.8 0 22 289 36 734.8 0 36 290 54 734.8 0 54 291 30 734.8 0 30 292 33 734.8 0 33 293 28 734.8 0 28 294 36 734.8 0 36 295 32 734.8 0 32 296 19 734.8 0 19 297 18 734.8 0 18 298 18 734.8 0 18 299 36 734.8 0 36 300 42 734.8 0 42 301 26 734.8 0 26 Finished in 22.38 s (39 us/read; 1.54 M reads/minute). === Summary === Total reads processed: 573,043 Reads with adapters: 7,187 (1.3%) Reads that were too short: 66 (0.0%) Reads written (passing filters): 7,121 (1.2%) Total basepairs processed: 146,260,403 bp Total written (filtered): 1,951,795 bp (1.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7187 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 32 0.0 3 0 32 17 6937 0.0 3 6884 51 1 1 18 15 0.0 3 1 14 19 1 0.0 3 0 0 1 20 10 0.0 3 1 0 0 9 30 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 41 4 0.0 3 0 0 0 4 48 3 0.0 3 3 84 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 127 3 0.0 3 0 0 0 3 131 3 0.0 3 0 0 0 3 132 23 0.0 3 0 0 0 23 133 13 0.0 3 0 0 0 13 134 1 0.0 3 0 0 0 1 135 6 0.0 3 0 0 0 6 136 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 60 0.0 3 0 0 0 60 163 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 185 3 0.0 3 0 0 0 3 186 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 194 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 234 22 0.0 3 0 0 1 21 235 1 0.0 3 0 0 0 1 247 2 0.0 3 0 0 0 2 249 9 0.0 3 0 0 0 9 250 9 0.0 3 0 0 0 9 256 4 0.0 3 0 0 1 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.26 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 7,121 Reads with adapters: 493 (6.9%) Reads written (passing filters): 7,121 (100.0%) Total basepairs processed: 1,951,795 bp Total written (filtered): 1,950,315 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 493 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 38.1% C: 56.6% G: 4.9% T: 0.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 492 111.3 0 492 4 1 27.8 0 1