This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 79.80 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 391,560 (13.0%) Reads that were too short: 145,249 (4.8%) Reads written (passing filters): 246,311 (8.2%) Total basepairs processed: 905,907,359 bp Total written (filtered): 47,612,893 bp (5.3%) === Adapter 1 === Sequence: AAGACA; Type: regular 5'; Length: 6; Trimmed: 391560 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 629 734.8 0 629 7 45857 734.8 0 45857 8 122 734.8 0 122 9 4885 734.8 0 4885 10 40 734.8 0 40 11 31 734.8 0 31 12 20 734.8 0 20 13 19 734.8 0 19 14 9 734.8 0 9 15 16 734.8 0 16 16 10 734.8 0 10 17 16 734.8 0 16 18 12 734.8 0 12 19 16 734.8 0 16 20 9 734.8 0 9 21 17 734.8 0 17 22 20 734.8 0 20 23 24 734.8 0 24 24 29 734.8 0 29 25 11 734.8 0 11 26 18 734.8 0 18 27 18 734.8 0 18 28 20 734.8 0 20 29 25 734.8 0 25 30 22 734.8 0 22 31 14 734.8 0 14 32 10 734.8 0 10 33 15 734.8 0 15 34 10 734.8 0 10 35 21 734.8 0 21 36 27 734.8 0 27 37 21 734.8 0 21 38 19 734.8 0 19 39 21 734.8 0 21 40 245 734.8 0 245 41 22 734.8 0 22 42 16 734.8 0 16 43 16 734.8 0 16 44 12 734.8 0 12 45 14 734.8 0 14 46 10 734.8 0 10 47 17 734.8 0 17 48 19 734.8 0 19 49 21 734.8 0 21 50 20 734.8 0 20 51 23 734.8 0 23 52 19 734.8 0 19 53 33 734.8 0 33 54 131 734.8 0 131 55 33 734.8 0 33 56 33 734.8 0 33 57 18 734.8 0 18 58 56 734.8 0 56 59 57 734.8 0 57 60 27 734.8 0 27 61 25 734.8 0 25 62 37 734.8 0 37 63 277 734.8 0 277 64 60 734.8 0 60 65 80 734.8 0 80 66 30 734.8 0 30 67 27 734.8 0 27 68 34 734.8 0 34 69 304 734.8 0 304 70 112 734.8 0 112 71 696 734.8 0 696 72 1306 734.8 0 1306 73 2163 734.8 0 2163 74 2142 734.8 0 2142 75 788 734.8 0 788 76 957 734.8 0 957 77 1302 734.8 0 1302 78 1469 734.8 0 1469 79 2099 734.8 0 2099 80 1694 734.8 0 1694 81 1284 734.8 0 1284 82 1944 734.8 0 1944 83 2423 734.8 0 2423 84 1565 734.8 0 1565 85 2397 734.8 0 2397 86 3181 734.8 0 3181 87 2686 734.8 0 2686 88 1704 734.8 0 1704 89 2403 734.8 0 2403 90 744 734.8 0 744 91 1132 734.8 0 1132 92 2577 734.8 0 2577 93 1191 734.8 0 1191 94 1362 734.8 0 1362 95 1263 734.8 0 1263 96 724 734.8 0 724 97 435 734.8 0 435 98 613 734.8 0 613 99 723 734.8 0 723 100 1395 734.8 0 1395 101 1500 734.8 0 1500 102 1357 734.8 0 1357 103 1620 734.8 0 1620 104 1244 734.8 0 1244 105 869 734.8 0 869 106 477 734.8 0 477 107 524 734.8 0 524 108 1245 734.8 0 1245 109 1465 734.8 0 1465 110 1866 734.8 0 1866 111 1601 734.8 0 1601 112 1573 734.8 0 1573 113 1254 734.8 0 1254 114 1191 734.8 0 1191 115 1101 734.8 0 1101 116 1758 734.8 0 1758 117 1818 734.8 0 1818 118 2011 734.8 0 2011 119 1894 734.8 0 1894 120 1990 734.8 0 1990 121 1912 734.8 0 1912 122 1744 734.8 0 1744 123 1587 734.8 0 1587 124 1469 734.8 0 1469 125 1576 734.8 0 1576 126 1282 734.8 0 1282 127 1100 734.8 0 1100 128 1139 734.8 0 1139 129 1392 734.8 0 1392 130 1227 734.8 0 1227 131 1753 734.8 0 1753 132 1481 734.8 0 1481 133 1135 734.8 0 1135 134 1168 734.8 0 1168 135 1158 734.8 0 1158 136 1169 734.8 0 1169 137 1256 734.8 0 1256 138 1500 734.8 0 1500 139 1387 734.8 0 1387 140 1178 734.8 0 1178 141 1309 734.8 0 1309 142 1748 734.8 0 1748 143 1468 734.8 0 1468 144 1302 734.8 0 1302 145 1626 734.8 0 1626 146 1617 734.8 0 1617 147 1479 734.8 0 1479 148 1556 734.8 0 1556 149 1207 734.8 0 1207 150 1416 734.8 0 1416 151 1329 734.8 0 1329 152 1815 734.8 0 1815 153 1645 734.8 0 1645 154 1173 734.8 0 1173 155 1279 734.8 0 1279 156 1125 734.8 0 1125 157 1212 734.8 0 1212 158 1351 734.8 0 1351 159 1165 734.8 0 1165 160 1354 734.8 0 1354 161 1111 734.8 0 1111 162 1297 734.8 0 1297 163 1058 734.8 0 1058 164 952 734.8 0 952 165 929 734.8 0 929 166 1793 734.8 0 1793 167 4265 734.8 0 4265 168 1629 734.8 0 1629 169 1294 734.8 0 1294 170 3292 734.8 0 3292 171 3302 734.8 0 3302 172 1772 734.8 0 1772 173 1201 734.8 0 1201 174 1027 734.8 0 1027 175 1406 734.8 0 1406 176 1119 734.8 0 1119 177 1322 734.8 0 1322 178 1318 734.8 0 1318 179 1187 734.8 0 1187 180 1418 734.8 0 1418 181 2085 734.8 0 2085 182 2259 734.8 0 2259 183 2052 734.8 0 2052 184 1414 734.8 0 1414 185 1264 734.8 0 1264 186 1107 734.8 0 1107 187 1312 734.8 0 1312 188 1358 734.8 0 1358 189 1165 734.8 0 1165 190 1258 734.8 0 1258 191 1243 734.8 0 1243 192 1074 734.8 0 1074 193 1193 734.8 0 1193 194 875 734.8 0 875 195 1162 734.8 0 1162 196 973 734.8 0 973 197 1578 734.8 0 1578 198 1941 734.8 0 1941 199 1468 734.8 0 1468 200 1529 734.8 0 1529 201 919 734.8 0 919 202 1010 734.8 0 1010 203 1300 734.8 0 1300 204 1325 734.8 0 1325 205 1271 734.8 0 1271 206 1192 734.8 0 1192 207 997 734.8 0 997 208 867 734.8 0 867 209 881 734.8 0 881 210 936 734.8 0 936 211 1084 734.8 0 1084 212 1089 734.8 0 1089 213 1838 734.8 0 1838 214 1963 734.8 0 1963 215 1279 734.8 0 1279 216 950 734.8 0 950 217 985 734.8 0 985 218 1365 734.8 0 1365 219 1536 734.8 0 1536 220 1002 734.8 0 1002 221 885 734.8 0 885 222 908 734.8 0 908 223 1158 734.8 0 1158 224 1040 734.8 0 1040 225 1006 734.8 0 1006 226 979 734.8 0 979 227 1000 734.8 0 1000 228 1107 734.8 0 1107 229 1195 734.8 0 1195 230 888 734.8 0 888 231 891 734.8 0 891 232 967 734.8 0 967 233 1070 734.8 0 1070 234 1020 734.8 0 1020 235 999 734.8 0 999 236 706 734.8 0 706 237 1040 734.8 0 1040 238 1239 734.8 0 1239 239 1576 734.8 0 1576 240 1244 734.8 0 1244 241 1135 734.8 0 1135 242 1242 734.8 0 1242 243 1294 734.8 0 1294 244 1021 734.8 0 1021 245 935 734.8 0 935 246 1271 734.8 0 1271 247 1033 734.8 0 1033 248 1229 734.8 0 1229 249 1767 734.8 0 1767 250 1256 734.8 0 1256 251 1157 734.8 0 1157 252 1565 734.8 0 1565 253 1163 734.8 0 1163 254 875 734.8 0 875 255 763 734.8 0 763 256 818 734.8 0 818 257 783 734.8 0 783 258 748 734.8 0 748 259 792 734.8 0 792 260 1223 734.8 0 1223 261 1531 734.8 0 1531 262 2204 734.8 0 2204 263 2253 734.8 0 2253 264 1445 734.8 0 1445 265 1029 734.8 0 1029 266 1048 734.8 0 1048 267 3053 734.8 0 3053 268 8154 734.8 0 8154 269 3627 734.8 0 3627 270 1485 734.8 0 1485 271 969 734.8 0 969 272 1376 734.8 0 1376 273 1230 734.8 0 1230 274 931 734.8 0 931 275 903 734.8 0 903 276 875 734.8 0 875 277 1209 734.8 0 1209 278 1813 734.8 0 1813 279 1648 734.8 0 1648 280 5987 734.8 0 5987 281 4755 734.8 0 4755 282 2163 734.8 0 2163 283 1070 734.8 0 1070 284 1144 734.8 0 1144 285 936 734.8 0 936 286 1176 734.8 0 1176 287 1321 734.8 0 1321 288 942 734.8 0 942 289 1128 734.8 0 1128 290 1536 734.8 0 1536 291 1460 734.8 0 1460 292 1621 734.8 0 1621 293 1264 734.8 0 1264 294 1073 734.8 0 1073 295 1028 734.8 0 1028 296 1290 734.8 0 1290 297 1491 734.8 0 1491 298 1835 734.8 0 1835 299 4061 734.8 0 4061 300 3304 734.8 0 3304 301 2023 734.8 0 2023 Finished in 9.47 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 246,311 Reads with adapters: 46,525 (18.9%) Reads that were too short: 105 (0.0%) Reads written (passing filters): 46,420 (18.8%) Total basepairs processed: 47,612,893 bp Total written (filtered): 12,709,447 bp (26.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 46525 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 23 0.0 3 0 0 19 3 1 19 472 0.0 3 0 451 14 5 2 20 45657 0.0 4 44737 792 89 29 10 21 129 0.0 4 34 93 2 22 15 0.0 4 2 0 10 0 3 23 32 0.0 4 0 1 0 29 2 24 4 0.0 4 1 0 0 0 3 25 3 0.0 4 1 0 0 0 2 27 2 0.0 4 0 0 0 0 2 28 1 0.0 4 0 0 0 0 1 30 2 0.0 4 0 0 0 0 2 34 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 39 2 0.0 4 0 0 0 0 2 40 3 0.0 4 0 0 0 0 3 42 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 48 7 0.0 4 0 0 0 0 7 52 2 0.0 4 0 0 0 0 2 54 1 0.0 4 0 0 0 0 1 56 2 0.0 4 0 0 0 0 2 57 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 62 2 0.0 4 0 0 0 0 2 63 1 0.0 4 0 0 0 1 67 2 0.0 4 0 0 0 0 2 69 3 0.0 4 0 0 0 0 3 70 6 0.0 4 0 0 0 0 6 71 4 0.0 4 0 0 0 0 4 72 2 0.0 4 0 0 0 0 2 73 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 1 80 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 1 89 3 0.0 4 0 0 0 1 2 93 1 0.0 4 0 0 0 0 1 95 4 0.0 4 0 0 0 0 4 96 2 0.0 4 0 0 0 0 2 97 1 0.0 4 0 0 0 0 1 98 3 0.0 4 0 0 0 0 3 99 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 0 2 101 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 111 29 0.0 4 0 0 0 26 3 112 1 0.0 4 0 0 0 0 1 113 3 0.0 4 0 0 0 0 3 114 3 0.0 4 0 0 0 0 3 116 3 0.0 4 0 0 0 0 3 118 1 0.0 4 0 0 0 0 1 120 2 0.0 4 0 0 0 0 2 123 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 4 0.0 4 0 0 0 0 4 129 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 133 3 0.0 4 0 0 0 0 3 134 3 0.0 4 0 0 0 0 3 136 1 0.0 4 0 0 0 0 1 139 4 0.0 4 0 0 0 1 3 140 2 0.0 4 0 0 0 0 2 141 2 0.0 4 0 0 0 0 2 142 1 0.0 4 0 0 0 0 1 143 4 0.0 4 0 0 0 0 4 144 1 0.0 4 0 0 0 0 1 148 2 0.0 4 0 0 0 0 2 150 1 0.0 4 0 0 0 0 1 151 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 156 2 0.0 4 0 0 0 0 2 157 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 160 2 0.0 4 0 0 0 0 2 162 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 178 2 0.0 4 0 0 0 0 2 182 1 0.0 4 0 0 0 0 1 185 2 0.0 4 0 0 0 0 2 189 1 0.0 4 0 0 0 1 195 1 0.0 4 0 0 0 0 1 196 2 0.0 4 0 0 0 1 1 201 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.64 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 46,420 Reads with adapters: 5,387 (11.6%) Reads written (passing filters): 46,420 (100.0%) Total basepairs processed: 12,709,447 bp Total written (filtered): 12,646,679 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5387 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.0% C: 3.6% G: 92.6% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 13 725.3 0 13 4 2 181.3 0 2 6 1 11.3 0 1 9 1 0.2 0 1 10 5 0.0 1 1 4 11 5283 0.0 1 29 5254 12 59 0.0 1 1 58 13 1 0.0 1 0 1 14 1 0.0 1 0 1 15 2 0.0 1 0 2 16 1 0.0 1 0 1 79 1 0.0 1 1 124 1 0.0 1 0 1 152 3 0.0 1 3 191 1 0.0 1 0 1 210 4 0.0 1 3 1 225 1 0.0 1 1 260 2 0.0 1 2 261 3 0.0 1 3 270 1 0.0 1 1 274 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.