This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.92 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 34,903 (1.2%) Reads that were too short: 5,861 (0.2%) Reads written (passing filters): 29,042 (1.0%) Total basepairs processed: 905,907,359 bp Total written (filtered): 6,850,343 bp (0.8%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 34903 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 472 734.8 0 472 7 247 734.8 0 247 8 3766 734.8 0 3766 9 10401 734.8 0 10401 10 64 734.8 0 64 11 29 734.8 0 29 12 33 734.8 0 33 13 20 734.8 0 20 14 26 734.8 0 26 15 33 734.8 0 33 16 28 734.8 0 28 17 26 734.8 0 26 18 21 734.8 0 21 19 31 734.8 0 31 20 40 734.8 0 40 21 21 734.8 0 21 22 25 734.8 0 25 23 20 734.8 0 20 24 29 734.8 0 29 25 27 734.8 0 27 26 28 734.8 0 28 27 21 734.8 0 21 28 37 734.8 0 37 29 27 734.8 0 27 30 18 734.8 0 18 31 23 734.8 0 23 32 19 734.8 0 19 33 18 734.8 0 18 34 32 734.8 0 32 35 29 734.8 0 29 36 42 734.8 0 42 37 34 734.8 0 34 38 28 734.8 0 28 39 36 734.8 0 36 40 37 734.8 0 37 41 23 734.8 0 23 42 39 734.8 0 39 43 26 734.8 0 26 44 22 734.8 0 22 45 28 734.8 0 28 46 26 734.8 0 26 47 26 734.8 0 26 48 24 734.8 0 24 49 43 734.8 0 43 50 30 734.8 0 30 51 35 734.8 0 35 52 56 734.8 0 56 53 46 734.8 0 46 54 31 734.8 0 31 55 31 734.8 0 31 56 39 734.8 0 39 57 28 734.8 0 28 58 39 734.8 0 39 59 32 734.8 0 32 60 34 734.8 0 34 61 39 734.8 0 39 62 28 734.8 0 28 63 42 734.8 0 42 64 74 734.8 0 74 65 42 734.8 0 42 66 24 734.8 0 24 67 52 734.8 0 52 68 35 734.8 0 35 69 45 734.8 0 45 70 38 734.8 0 38 71 46 734.8 0 46 72 84 734.8 0 84 73 689 734.8 0 689 74 1611 734.8 0 1611 75 160 734.8 0 160 76 20 734.8 0 20 77 21 734.8 0 21 78 33 734.8 0 33 79 28 734.8 0 28 80 24 734.8 0 24 81 25 734.8 0 25 82 35 734.8 0 35 83 37 734.8 0 37 84 38 734.8 0 38 85 58 734.8 0 58 86 106 734.8 0 106 87 74 734.8 0 74 88 97 734.8 0 97 89 83 734.8 0 83 90 32 734.8 0 32 91 36 734.8 0 36 92 40 734.8 0 40 93 33 734.8 0 33 94 37 734.8 0 37 95 51 734.8 0 51 96 30 734.8 0 30 97 37 734.8 0 37 98 29 734.8 0 29 99 36 734.8 0 36 100 22 734.8 0 22 101 33 734.8 0 33 102 35 734.8 0 35 103 25 734.8 0 25 104 29 734.8 0 29 105 31 734.8 0 31 106 21 734.8 0 21 107 26 734.8 0 26 108 41 734.8 0 41 109 70 734.8 0 70 110 18 734.8 0 18 111 37 734.8 0 37 112 18 734.8 0 18 113 21 734.8 0 21 114 20 734.8 0 20 115 53 734.8 0 53 116 36 734.8 0 36 117 25 734.8 0 25 118 29 734.8 0 29 119 32 734.8 0 32 120 35 734.8 0 35 121 42 734.8 0 42 122 36 734.8 0 36 123 28 734.8 0 28 124 27 734.8 0 27 125 30 734.8 0 30 126 33 734.8 0 33 127 36 734.8 0 36 128 37 734.8 0 37 129 55 734.8 0 55 130 53 734.8 0 53 131 31 734.8 0 31 132 33 734.8 0 33 133 34 734.8 0 34 134 47 734.8 0 47 135 62 734.8 0 62 136 97 734.8 0 97 137 124 734.8 0 124 138 61 734.8 0 61 139 37 734.8 0 37 140 52 734.8 0 52 141 77 734.8 0 77 142 53 734.8 0 53 143 46 734.8 0 46 144 38 734.8 0 38 145 55 734.8 0 55 146 100 734.8 0 100 147 138 734.8 0 138 148 112 734.8 0 112 149 117 734.8 0 117 150 55 734.8 0 55 151 37 734.8 0 37 152 38 734.8 0 38 153 64 734.8 0 64 154 61 734.8 0 61 155 120 734.8 0 120 156 137 734.8 0 137 157 82 734.8 0 82 158 185 734.8 0 185 159 156 734.8 0 156 160 137 734.8 0 137 161 94 734.8 0 94 162 71 734.8 0 71 163 41 734.8 0 41 164 36 734.8 0 36 165 56 734.8 0 56 166 133 734.8 0 133 167 139 734.8 0 139 168 69 734.8 0 69 169 59 734.8 0 59 170 127 734.8 0 127 171 71 734.8 0 71 172 58 734.8 0 58 173 66 734.8 0 66 174 489 734.8 0 489 175 648 734.8 0 648 176 130 734.8 0 130 177 71 734.8 0 71 178 29 734.8 0 29 179 37 734.8 0 37 180 62 734.8 0 62 181 84 734.8 0 84 182 96 734.8 0 96 183 85 734.8 0 85 184 75 734.8 0 75 185 113 734.8 0 113 186 160 734.8 0 160 187 1073 734.8 0 1073 188 381 734.8 0 381 189 77 734.8 0 77 190 37 734.8 0 37 191 50 734.8 0 50 192 55 734.8 0 55 193 34 734.8 0 34 194 56 734.8 0 56 195 40 734.8 0 40 196 38 734.8 0 38 197 39 734.8 0 39 198 28 734.8 0 28 199 29 734.8 0 29 200 51 734.8 0 51 201 40 734.8 0 40 202 42 734.8 0 42 203 53 734.8 0 53 204 88 734.8 0 88 205 99 734.8 0 99 206 68 734.8 0 68 207 51 734.8 0 51 208 49 734.8 0 49 209 37 734.8 0 37 210 43 734.8 0 43 211 36 734.8 0 36 212 27 734.8 0 27 213 42 734.8 0 42 214 47 734.8 0 47 215 73 734.8 0 73 216 40 734.8 0 40 217 35 734.8 0 35 218 45 734.8 0 45 219 44 734.8 0 44 220 47 734.8 0 47 221 46 734.8 0 46 222 41 734.8 0 41 223 54 734.8 0 54 224 52 734.8 0 52 225 44 734.8 0 44 226 39 734.8 0 39 227 133 734.8 0 133 228 94 734.8 0 94 229 59 734.8 0 59 230 37 734.8 0 37 231 64 734.8 0 64 232 57 734.8 0 57 233 37 734.8 0 37 234 48 734.8 0 48 235 46 734.8 0 46 236 41 734.8 0 41 237 43 734.8 0 43 238 70 734.8 0 70 239 34 734.8 0 34 240 40 734.8 0 40 241 35 734.8 0 35 242 30 734.8 0 30 243 29 734.8 0 29 244 28 734.8 0 28 245 43 734.8 0 43 246 41 734.8 0 41 247 54 734.8 0 54 248 66 734.8 0 66 249 63 734.8 0 63 250 49 734.8 0 49 251 136 734.8 0 136 252 279 734.8 0 279 253 188 734.8 0 188 254 164 734.8 0 164 255 83 734.8 0 83 256 40 734.8 0 40 257 58 734.8 0 58 258 60 734.8 0 60 259 41 734.8 0 41 260 39 734.8 0 39 261 109 734.8 0 109 262 208 734.8 0 208 263 215 734.8 0 215 264 64 734.8 0 64 265 40 734.8 0 40 266 30 734.8 0 30 267 41 734.8 0 41 268 45 734.8 0 45 269 35 734.8 0 35 270 24 734.8 0 24 271 15 734.8 0 15 272 41 734.8 0 41 273 59 734.8 0 59 274 45 734.8 0 45 275 33 734.8 0 33 276 31 734.8 0 31 277 47 734.8 0 47 278 101 734.8 0 101 279 41 734.8 0 41 280 38 734.8 0 38 281 69 734.8 0 69 282 95 734.8 0 95 283 53 734.8 0 53 284 18 734.8 0 18 285 68 734.8 0 68 286 70 734.8 0 70 287 34 734.8 0 34 288 37 734.8 0 37 289 23 734.8 0 23 290 37 734.8 0 37 291 55 734.8 0 55 292 54 734.8 0 54 293 29 734.8 0 29 294 54 734.8 0 54 295 51 734.8 0 51 296 33 734.8 0 33 297 53 734.8 0 53 298 40 734.8 0 40 299 50 734.8 0 50 300 63 734.8 0 63 301 42 734.8 0 42 Finished in 1.34 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 29,042 Reads with adapters: 11,103 (38.2%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 11,098 (38.2%) Total basepairs processed: 6,850,343 bp Total written (filtered): 3,008,215 bp (43.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11103 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 8 0.0 3 0 0 5 1 2 19 148 0.0 3 0 136 8 3 1 20 10507 0.0 4 9883 528 75 11 10 21 29 0.0 4 4 23 1 1 22 1 0.0 4 0 1 23 292 0.0 4 273 9 0 6 4 24 1 0.0 4 1 57 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 99 23 0.0 4 0 0 0 0 23 110 1 0.0 4 0 0 0 0 1 111 81 0.0 4 0 0 0 73 8 127 5 0.0 4 0 0 0 0 5 155 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 11,098 Reads with adapters: 640 (5.8%) Reads written (passing filters): 11,098 (100.0%) Total basepairs processed: 3,008,215 bp Total written (filtered): 2,997,693 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 640 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.4% C: 3.9% G: 85.9% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 43.4 0 1 8 1 0.2 0 1 9 105 0.0 0 9 96 10 393 0.0 1 26 367 11 120 0.0 1 0 120 12 1 0.0 1 0 1 208 10 0.0 1 8 2 210 1 0.0 1 1 211 1 0.0 1 1 257 6 0.0 1 6 260 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.