This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.27 s (25 us/read; 2.40 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 933,864 (31.0%) Reads that were too short: 882,125 (29.3%) Reads written (passing filters): 51,739 (1.7%) Total basepairs processed: 905,907,359 bp Total written (filtered): 9,267,352 bp (1.0%) === Adapter 1 === Sequence: CCGGAA; Type: regular 5'; Length: 6; Trimmed: 933864 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 120 734.8 0 120 7 403 734.8 0 403 8 410 734.8 0 410 9 10491 734.8 0 10491 10 32 734.8 0 32 11 12 734.8 0 12 12 26 734.8 0 26 13 16 734.8 0 16 14 12 734.8 0 12 15 10 734.8 0 10 16 29 734.8 0 29 17 11 734.8 0 11 18 15 734.8 0 15 19 16 734.8 0 16 20 22 734.8 0 22 21 25 734.8 0 25 22 15 734.8 0 15 23 27 734.8 0 27 24 13 734.8 0 13 25 21 734.8 0 21 26 9 734.8 0 9 27 18 734.8 0 18 28 19 734.8 0 19 29 17 734.8 0 17 30 24 734.8 0 24 31 15 734.8 0 15 32 12 734.8 0 12 33 36 734.8 0 36 34 32 734.8 0 32 35 47 734.8 0 47 36 51 734.8 0 51 37 2103 734.8 0 2103 38 43 734.8 0 43 39 37 734.8 0 37 40 23 734.8 0 23 41 20 734.8 0 20 42 12 734.8 0 12 43 34 734.8 0 34 44 27 734.8 0 27 45 38 734.8 0 38 46 73 734.8 0 73 47 114 734.8 0 114 48 42 734.8 0 42 49 32 734.8 0 32 50 53 734.8 0 53 51 25 734.8 0 25 52 22 734.8 0 22 53 13 734.8 0 13 54 38 734.8 0 38 55 30 734.8 0 30 56 10 734.8 0 10 57 23 734.8 0 23 58 46 734.8 0 46 59 54 734.8 0 54 60 76 734.8 0 76 61 35 734.8 0 35 62 18 734.8 0 18 63 23 734.8 0 23 64 36 734.8 0 36 65 24 734.8 0 24 66 22 734.8 0 22 67 13 734.8 0 13 68 14 734.8 0 14 69 14 734.8 0 14 70 18 734.8 0 18 71 12 734.8 0 12 72 21 734.8 0 21 73 40 734.8 0 40 74 37 734.8 0 37 75 38 734.8 0 38 76 29 734.8 0 29 77 217 734.8 0 217 78 253 734.8 0 253 79 90 734.8 0 90 80 21 734.8 0 21 81 16 734.8 0 16 82 19 734.8 0 19 83 24 734.8 0 24 84 26 734.8 0 26 85 22 734.8 0 22 86 28 734.8 0 28 87 28 734.8 0 28 88 22 734.8 0 22 89 20 734.8 0 20 90 24 734.8 0 24 91 32 734.8 0 32 92 25 734.8 0 25 93 50 734.8 0 50 94 221 734.8 0 221 95 181 734.8 0 181 96 91 734.8 0 91 97 110 734.8 0 110 98 56 734.8 0 56 99 29 734.8 0 29 100 25 734.8 0 25 101 17 734.8 0 17 102 35 734.8 0 35 103 38 734.8 0 38 104 48 734.8 0 48 105 25 734.8 0 25 106 29 734.8 0 29 107 24 734.8 0 24 108 23 734.8 0 23 109 42 734.8 0 42 110 27 734.8 0 27 111 18 734.8 0 18 112 31 734.8 0 31 113 17 734.8 0 17 114 39 734.8 0 39 115 86 734.8 0 86 116 287 734.8 0 287 117 120 734.8 0 120 118 56 734.8 0 56 119 39 734.8 0 39 120 31 734.8 0 31 121 29 734.8 0 29 122 29 734.8 0 29 123 49 734.8 0 49 124 75 734.8 0 75 125 40 734.8 0 40 126 47 734.8 0 47 127 41 734.8 0 41 128 39 734.8 0 39 129 38 734.8 0 38 130 32 734.8 0 32 131 45 734.8 0 45 132 79 734.8 0 79 133 87 734.8 0 87 134 133 734.8 0 133 135 476 734.8 0 476 136 3496 734.8 0 3496 137 4451 734.8 0 4451 138 1265 734.8 0 1265 139 166 734.8 0 166 140 48 734.8 0 48 141 67 734.8 0 67 142 63 734.8 0 63 143 36 734.8 0 36 144 36 734.8 0 36 145 36 734.8 0 36 146 37 734.8 0 37 147 41 734.8 0 41 148 27 734.8 0 27 149 25 734.8 0 25 150 40 734.8 0 40 151 41 734.8 0 41 152 28 734.8 0 28 153 35 734.8 0 35 154 42 734.8 0 42 155 56 734.8 0 56 156 86 734.8 0 86 157 73 734.8 0 73 158 72 734.8 0 72 159 73 734.8 0 73 160 187 734.8 0 187 161 1483 734.8 0 1483 162 1285 734.8 0 1285 163 183 734.8 0 183 164 41 734.8 0 41 165 61 734.8 0 61 166 92 734.8 0 92 167 86 734.8 0 86 168 55 734.8 0 55 169 48 734.8 0 48 170 30 734.8 0 30 171 31 734.8 0 31 172 61 734.8 0 61 173 53 734.8 0 53 174 108 734.8 0 108 175 139 734.8 0 139 176 90 734.8 0 90 177 135 734.8 0 135 178 244 734.8 0 244 179 61 734.8 0 61 180 67 734.8 0 67 181 87 734.8 0 87 182 92 734.8 0 92 183 156 734.8 0 156 184 146 734.8 0 146 185 177 734.8 0 177 186 170 734.8 0 170 187 311 734.8 0 311 188 565 734.8 0 565 189 335 734.8 0 335 190 336 734.8 0 336 191 423 734.8 0 423 192 877 734.8 0 877 193 1351 734.8 0 1351 194 1264 734.8 0 1264 195 1010 734.8 0 1010 196 851 734.8 0 851 197 1206 734.8 0 1206 198 1260 734.8 0 1260 199 1397 734.8 0 1397 200 1969 734.8 0 1969 201 3587 734.8 0 3587 202 18380 734.8 0 18380 203 28182 734.8 0 28182 204 23488 734.8 0 23488 205 93639 734.8 0 93639 206 66110 734.8 0 66110 207 88767 734.8 0 88767 208 121274 734.8 0 121274 209 71880 734.8 0 71880 210 33231 734.8 0 33231 211 16563 734.8 0 16563 212 9646 734.8 0 9646 213 1472 734.8 0 1472 214 1668 734.8 0 1668 215 584 734.8 0 584 216 761 734.8 0 761 217 1173 734.8 0 1173 218 732 734.8 0 732 219 1075 734.8 0 1075 220 1485 734.8 0 1485 221 1268 734.8 0 1268 222 4705 734.8 0 4705 223 6448 734.8 0 6448 224 4209 734.8 0 4209 225 2386 734.8 0 2386 226 4561 734.8 0 4561 227 137400 734.8 0 137400 228 102844 734.8 0 102844 229 22778 734.8 0 22778 230 300 734.8 0 300 231 31 734.8 0 31 232 21 734.8 0 21 233 16 734.8 0 16 234 20 734.8 0 20 235 16 734.8 0 16 236 17 734.8 0 17 237 307 734.8 0 307 238 451 734.8 0 451 239 116 734.8 0 116 240 11 734.8 0 11 241 9 734.8 0 9 242 8 734.8 0 8 243 10 734.8 0 10 244 11 734.8 0 11 245 14 734.8 0 14 246 53 734.8 0 53 247 80 734.8 0 80 248 107 734.8 0 107 249 8 734.8 0 8 250 8 734.8 0 8 251 5 734.8 0 5 252 13 734.8 0 13 253 29 734.8 0 29 254 40 734.8 0 40 255 36 734.8 0 36 256 42 734.8 0 42 257 126 734.8 0 126 258 294 734.8 0 294 259 165 734.8 0 165 260 566 734.8 0 566 261 2628 734.8 0 2628 262 2911 734.8 0 2911 263 798 734.8 0 798 264 73 734.8 0 73 265 41 734.8 0 41 266 42 734.8 0 42 267 65 734.8 0 65 268 39 734.8 0 39 269 28 734.8 0 28 270 26 734.8 0 26 271 57 734.8 0 57 272 856 734.8 0 856 273 2979 734.8 0 2979 274 340 734.8 0 340 275 31 734.8 0 31 276 21 734.8 0 21 277 70 734.8 0 70 278 86 734.8 0 86 279 47 734.8 0 47 280 33 734.8 0 33 281 11 734.8 0 11 282 12 734.8 0 12 283 14 734.8 0 14 284 69 734.8 0 69 285 49 734.8 0 49 286 119 734.8 0 119 287 222 734.8 0 222 288 60 734.8 0 60 289 39 734.8 0 39 290 18 734.8 0 18 291 21 734.8 0 21 292 32 734.8 0 32 293 69 734.8 0 69 294 47 734.8 0 47 295 70 734.8 0 70 296 89 734.8 0 89 297 128 734.8 0 128 298 109 734.8 0 109 299 67 734.8 0 67 300 15 734.8 0 15 301 55 734.8 0 55 Finished in 1.86 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 51,739 Reads with adapters: 11,062 (21.4%) Reads that were too short: 21 (0.0%) Reads written (passing filters): 11,041 (21.3%) Total basepairs processed: 9,267,352 bp Total written (filtered): 3,033,391 bp (32.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11062 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 4 0.0 3 0 0 2 2 16 67 0.0 3 0 64 2 1 17 10845 0.0 3 10634 206 5 18 43 0.0 3 4 27 11 1 19 19 0.0 3 0 0 10 9 20 24 0.0 3 1 0 0 23 21 1 0.0 3 1 22 2 0.0 3 1 0 1 26 3 0.0 3 0 0 0 3 28 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 31 3 0.0 3 0 0 0 3 34 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 19 0.0 3 0 0 0 19 69 1 0.0 3 0 0 0 1 76 9 0.0 3 0 0 0 9 77 1 0.0 3 0 0 0 1 79 3 0.0 3 0 0 0 3 84 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.40 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 11,041 Reads with adapters: 702 (6.4%) Reads written (passing filters): 11,041 (100.0%) Total basepairs processed: 3,033,391 bp Total written (filtered): 3,031,282 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 702 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 70.4% C: 6.6% G: 22.6% T: 0.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 699 172.5 0 699 4 3 43.1 0 3