This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 80.57 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 375,564 (12.5%) Reads that were too short: 49,190 (1.6%) Reads written (passing filters): 326,374 (10.8%) Total basepairs processed: 905,907,359 bp Total written (filtered): 87,496,254 bp (9.7%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 375564 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2459 734.8 0 2459 7 257844 734.8 0 257844 8 8284 734.8 0 8284 9 28 734.8 0 28 10 33 734.8 0 33 11 26 734.8 0 26 12 96 734.8 0 96 13 286 734.8 0 286 14 153 734.8 0 153 15 68 734.8 0 68 16 22 734.8 0 22 17 22 734.8 0 22 18 6 734.8 0 6 19 15 734.8 0 15 20 10 734.8 0 10 21 11 734.8 0 11 22 13 734.8 0 13 23 21 734.8 0 21 24 21 734.8 0 21 25 38 734.8 0 38 26 47 734.8 0 47 27 34 734.8 0 34 28 15 734.8 0 15 29 18 734.8 0 18 30 16 734.8 0 16 31 10 734.8 0 10 32 6 734.8 0 6 33 21 734.8 0 21 34 23 734.8 0 23 35 8 734.8 0 8 36 16 734.8 0 16 37 8 734.8 0 8 38 75 734.8 0 75 39 22 734.8 0 22 40 14 734.8 0 14 41 6 734.8 0 6 42 45 734.8 0 45 43 12 734.8 0 12 44 15 734.8 0 15 45 14 734.8 0 14 46 12 734.8 0 12 47 18 734.8 0 18 48 12 734.8 0 12 49 20 734.8 0 20 50 16 734.8 0 16 51 18 734.8 0 18 52 10 734.8 0 10 53 22 734.8 0 22 54 24 734.8 0 24 55 18 734.8 0 18 56 14 734.8 0 14 57 26 734.8 0 26 58 4 734.8 0 4 59 17 734.8 0 17 60 38 734.8 0 38 61 50 734.8 0 50 62 18 734.8 0 18 63 22 734.8 0 22 64 15 734.8 0 15 65 11 734.8 0 11 66 15 734.8 0 15 67 11 734.8 0 11 68 15 734.8 0 15 69 13 734.8 0 13 70 21 734.8 0 21 71 62 734.8 0 62 72 15 734.8 0 15 73 28 734.8 0 28 74 32 734.8 0 32 75 101 734.8 0 101 76 146 734.8 0 146 77 63 734.8 0 63 78 125 734.8 0 125 79 107 734.8 0 107 80 206 734.8 0 206 81 209 734.8 0 209 82 485 734.8 0 485 83 277 734.8 0 277 84 149 734.8 0 149 85 253 734.8 0 253 86 117 734.8 0 117 87 305 734.8 0 305 88 482 734.8 0 482 89 556 734.8 0 556 90 345 734.8 0 345 91 327 734.8 0 327 92 62 734.8 0 62 93 191 734.8 0 191 94 391 734.8 0 391 95 271 734.8 0 271 96 270 734.8 0 270 97 245 734.8 0 245 98 223 734.8 0 223 99 175 734.8 0 175 100 154 734.8 0 154 101 134 734.8 0 134 102 289 734.8 0 289 103 367 734.8 0 367 104 149 734.8 0 149 105 175 734.8 0 175 106 187 734.8 0 187 107 197 734.8 0 197 108 66 734.8 0 66 109 79 734.8 0 79 110 133 734.8 0 133 111 319 734.8 0 319 112 379 734.8 0 379 113 279 734.8 0 279 114 315 734.8 0 315 115 198 734.8 0 198 116 150 734.8 0 150 117 140 734.8 0 140 118 310 734.8 0 310 119 392 734.8 0 392 120 414 734.8 0 414 121 294 734.8 0 294 122 240 734.8 0 240 123 326 734.8 0 326 124 312 734.8 0 312 125 226 734.8 0 226 126 344 734.8 0 344 127 340 734.8 0 340 128 267 734.8 0 267 129 250 734.8 0 250 130 239 734.8 0 239 131 199 734.8 0 199 132 272 734.8 0 272 133 320 734.8 0 320 134 310 734.8 0 310 135 288 734.8 0 288 136 238 734.8 0 238 137 285 734.8 0 285 138 315 734.8 0 315 139 452 734.8 0 452 140 652 734.8 0 652 141 441 734.8 0 441 142 236 734.8 0 236 143 217 734.8 0 217 144 349 734.8 0 349 145 311 734.8 0 311 146 364 734.8 0 364 147 322 734.8 0 322 148 312 734.8 0 312 149 184 734.8 0 184 150 229 734.8 0 229 151 272 734.8 0 272 152 308 734.8 0 308 153 354 734.8 0 354 154 320 734.8 0 320 155 328 734.8 0 328 156 291 734.8 0 291 157 304 734.8 0 304 158 401 734.8 0 401 159 439 734.8 0 439 160 1159 734.8 0 1159 161 1283 734.8 0 1283 162 594 734.8 0 594 163 344 734.8 0 344 164 306 734.8 0 306 165 278 734.8 0 278 166 197 734.8 0 197 167 221 734.8 0 221 168 322 734.8 0 322 169 369 734.8 0 369 170 285 734.8 0 285 171 245 734.8 0 245 172 272 734.8 0 272 173 365 734.8 0 365 174 289 734.8 0 289 175 270 734.8 0 270 176 327 734.8 0 327 177 368 734.8 0 368 178 303 734.8 0 303 179 294 734.8 0 294 180 520 734.8 0 520 181 572 734.8 0 572 182 667 734.8 0 667 183 419 734.8 0 419 184 400 734.8 0 400 185 502 734.8 0 502 186 663 734.8 0 663 187 6076 734.8 0 6076 188 9500 734.8 0 9500 189 1710 734.8 0 1710 190 367 734.8 0 367 191 289 734.8 0 289 192 225 734.8 0 225 193 324 734.8 0 324 194 204 734.8 0 204 195 266 734.8 0 266 196 414 734.8 0 414 197 471 734.8 0 471 198 249 734.8 0 249 199 236 734.8 0 236 200 257 734.8 0 257 201 416 734.8 0 416 202 801 734.8 0 801 203 496 734.8 0 496 204 366 734.8 0 366 205 313 734.8 0 313 206 528 734.8 0 528 207 476 734.8 0 476 208 375 734.8 0 375 209 362 734.8 0 362 210 477 734.8 0 477 211 606 734.8 0 606 212 425 734.8 0 425 213 383 734.8 0 383 214 378 734.8 0 378 215 334 734.8 0 334 216 324 734.8 0 324 217 286 734.8 0 286 218 315 734.8 0 315 219 429 734.8 0 429 220 467 734.8 0 467 221 453 734.8 0 453 222 469 734.8 0 469 223 395 734.8 0 395 224 332 734.8 0 332 225 320 734.8 0 320 226 310 734.8 0 310 227 375 734.8 0 375 228 328 734.8 0 328 229 371 734.8 0 371 230 752 734.8 0 752 231 897 734.8 0 897 232 544 734.8 0 544 233 360 734.8 0 360 234 422 734.8 0 422 235 318 734.8 0 318 236 176 734.8 0 176 237 269 734.8 0 269 238 420 734.8 0 420 239 487 734.8 0 487 240 608 734.8 0 608 241 678 734.8 0 678 242 499 734.8 0 499 243 332 734.8 0 332 244 309 734.8 0 309 245 374 734.8 0 374 246 349 734.8 0 349 247 228 734.8 0 228 248 238 734.8 0 238 249 159 734.8 0 159 250 392 734.8 0 392 251 510 734.8 0 510 252 464 734.8 0 464 253 349 734.8 0 349 254 360 734.8 0 360 255 294 734.8 0 294 256 403 734.8 0 403 257 315 734.8 0 315 258 289 734.8 0 289 259 260 734.8 0 260 260 267 734.8 0 267 261 429 734.8 0 429 262 974 734.8 0 974 263 344 734.8 0 344 264 351 734.8 0 351 265 304 734.8 0 304 266 394 734.8 0 394 267 286 734.8 0 286 268 273 734.8 0 273 269 338 734.8 0 338 270 531 734.8 0 531 271 455 734.8 0 455 272 378 734.8 0 378 273 540 734.8 0 540 274 459 734.8 0 459 275 517 734.8 0 517 276 1151 734.8 0 1151 277 752 734.8 0 752 278 598 734.8 0 598 279 647 734.8 0 647 280 481 734.8 0 481 281 406 734.8 0 406 282 497 734.8 0 497 283 893 734.8 0 893 284 858 734.8 0 858 285 566 734.8 0 566 286 268 734.8 0 268 287 252 734.8 0 252 288 478 734.8 0 478 289 756 734.8 0 756 290 499 734.8 0 499 291 413 734.8 0 413 292 457 734.8 0 457 293 389 734.8 0 389 294 538 734.8 0 538 295 536 734.8 0 536 296 622 734.8 0 622 297 402 734.8 0 402 298 550 734.8 0 550 299 582 734.8 0 582 300 893 734.8 0 893 301 4317 734.8 0 4317 Finished in 15.87 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 326,374 Reads with adapters: 11,187 (3.4%) Reads that were too short: 80 (0.0%) Reads written (passing filters): 11,107 (3.4%) Total basepairs processed: 87,496,254 bp Total written (filtered): 2,728,454 bp (3.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11187 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 3 0.0 3 0 0 3 19 72 0.0 3 0 64 4 3 1 20 8121 0.0 4 7876 205 32 5 3 21 31 0.0 4 11 18 0 1 1 22 2 0.0 4 1 0 0 0 1 23 12 0.0 4 0 0 0 8 4 24 4 0.0 4 0 0 0 3 1 26 1 0.0 4 0 0 0 0 1 29 2 0.0 4 0 0 0 0 2 33 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 1 61 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 72 2 0.0 4 0 0 0 1 1 84 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 114 2 0.0 4 0 0 0 0 2 121 1 0.0 4 0 0 0 0 1 123 4 0.0 4 0 0 0 0 4 124 3 0.0 4 0 0 0 0 3 125 6 0.0 4 0 0 0 0 6 126 40 0.0 4 0 0 0 0 40 127 2775 0.0 4 0 0 0 0 2775 128 8 0.0 4 0 0 0 0 8 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 145 1 0.0 4 1 148 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 7 0.0 4 0 0 0 0 7 156 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 1 217 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 3 0.0 4 0 0 0 0 3 228 4 0.0 4 0 0 0 0 4 229 1 0.0 4 0 0 0 1 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 4 0.0 4 0 0 0 0 4 235 3 0.0 4 0 0 0 0 3 236 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 258 3 0.0 4 0 0 0 0 3 260 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 2 0.0 4 0 0 0 0 2 264 3 0.0 4 0 0 0 0 3 265 3 0.0 4 0 0 0 0 3 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 285 2 0.0 4 0 0 0 0 2 286 2 0.0 4 0 0 0 0 2 288 1 0.0 4 0 0 0 0 1 289 7 0.0 4 0 0 0 0 7 291 2 0.0 4 0 0 0 0 2 292 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 11,107 Reads with adapters: 996 (9.0%) Reads written (passing filters): 11,107 (100.0%) Total basepairs processed: 2,728,454 bp Total written (filtered): 2,708,924 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 996 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.9% C: 2.3% G: 89.9% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 173.5 0 4 4 1 43.4 0 1 10 48 0.0 1 2 46 11 907 0.0 1 6 901 135 1 0.0 1 1 209 4 0.0 1 3 1 259 25 0.0 1 24 1 261 1 0.0 1 1 269 1 0.0 1 1 270 3 0.0 1 3 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.