This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.71 s (25 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 83,796 (2.8%) Reads that were too short: 52,143 (1.7%) Reads written (passing filters): 31,653 (1.1%) Total basepairs processed: 905,907,359 bp Total written (filtered): 6,201,697 bp (0.7%) === Adapter 1 === Sequence: AACCGA; Type: regular 5'; Length: 6; Trimmed: 83796 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 75 734.8 0 75 7 91 734.8 0 91 8 8210 734.8 0 8210 9 39 734.8 0 39 10 12 734.8 0 12 11 19 734.8 0 19 12 22 734.8 0 22 13 11 734.8 0 11 14 7 734.8 0 7 15 11 734.8 0 11 16 24 734.8 0 24 17 18 734.8 0 18 18 15 734.8 0 15 19 11 734.8 0 11 20 39 734.8 0 39 21 25 734.8 0 25 22 10 734.8 0 10 23 20 734.8 0 20 24 36 734.8 0 36 25 19 734.8 0 19 26 12 734.8 0 12 27 20 734.8 0 20 28 23 734.8 0 23 29 19 734.8 0 19 30 14 734.8 0 14 31 20 734.8 0 20 32 17 734.8 0 17 33 26 734.8 0 26 34 15 734.8 0 15 35 50 734.8 0 50 36 29 734.8 0 29 37 11 734.8 0 11 38 28 734.8 0 28 39 14 734.8 0 14 40 11 734.8 0 11 41 9 734.8 0 9 42 16 734.8 0 16 43 16 734.8 0 16 44 25 734.8 0 25 45 25 734.8 0 25 46 14 734.8 0 14 47 19 734.8 0 19 48 24 734.8 0 24 49 23 734.8 0 23 50 15 734.8 0 15 51 13 734.8 0 13 52 30 734.8 0 30 53 22 734.8 0 22 54 14 734.8 0 14 55 13 734.8 0 13 56 21 734.8 0 21 57 28 734.8 0 28 58 20 734.8 0 20 59 10 734.8 0 10 60 20 734.8 0 20 61 26 734.8 0 26 62 18 734.8 0 18 63 24 734.8 0 24 64 89 734.8 0 89 65 14 734.8 0 14 66 13 734.8 0 13 67 20 734.8 0 20 68 25 734.8 0 25 69 18 734.8 0 18 70 26 734.8 0 26 71 26 734.8 0 26 72 41 734.8 0 41 73 27 734.8 0 27 74 18 734.8 0 18 75 29 734.8 0 29 76 35 734.8 0 35 77 51 734.8 0 51 78 50 734.8 0 50 79 59 734.8 0 59 80 67 734.8 0 67 81 132 734.8 0 132 82 142 734.8 0 142 83 213 734.8 0 213 84 357 734.8 0 357 85 184 734.8 0 184 86 63 734.8 0 63 87 49 734.8 0 49 88 37 734.8 0 37 89 63 734.8 0 63 90 80 734.8 0 80 91 68 734.8 0 68 92 158 734.8 0 158 93 220 734.8 0 220 94 194 734.8 0 194 95 338 734.8 0 338 96 115 734.8 0 115 97 61 734.8 0 61 98 75 734.8 0 75 99 109 734.8 0 109 100 123 734.8 0 123 101 83 734.8 0 83 102 53 734.8 0 53 103 114 734.8 0 114 104 83 734.8 0 83 105 28 734.8 0 28 106 31 734.8 0 31 107 141 734.8 0 141 108 165 734.8 0 165 109 134 734.8 0 134 110 118 734.8 0 118 111 102 734.8 0 102 112 100 734.8 0 100 113 151 734.8 0 151 114 147 734.8 0 147 115 223 734.8 0 223 116 253 734.8 0 253 117 141 734.8 0 141 118 138 734.8 0 138 119 151 734.8 0 151 120 151 734.8 0 151 121 113 734.8 0 113 122 104 734.8 0 104 123 165 734.8 0 165 124 183 734.8 0 183 125 186 734.8 0 186 126 651 734.8 0 651 127 483 734.8 0 483 128 221 734.8 0 221 129 117 734.8 0 117 130 104 734.8 0 104 131 112 734.8 0 112 132 120 734.8 0 120 133 159 734.8 0 159 134 259 734.8 0 259 135 223 734.8 0 223 136 220 734.8 0 220 137 239 734.8 0 239 138 165 734.8 0 165 139 133 734.8 0 133 140 199 734.8 0 199 141 140 734.8 0 140 142 103 734.8 0 103 143 134 734.8 0 134 144 162 734.8 0 162 145 185 734.8 0 185 146 154 734.8 0 154 147 122 734.8 0 122 148 140 734.8 0 140 149 111 734.8 0 111 150 141 734.8 0 141 151 119 734.8 0 119 152 150 734.8 0 150 153 249 734.8 0 249 154 205 734.8 0 205 155 250 734.8 0 250 156 208 734.8 0 208 157 272 734.8 0 272 158 258 734.8 0 258 159 135 734.8 0 135 160 178 734.8 0 178 161 121 734.8 0 121 162 123 734.8 0 123 163 131 734.8 0 131 164 241 734.8 0 241 165 241 734.8 0 241 166 122 734.8 0 122 167 116 734.8 0 116 168 150 734.8 0 150 169 207 734.8 0 207 170 241 734.8 0 241 171 174 734.8 0 174 172 254 734.8 0 254 173 451 734.8 0 451 174 259 734.8 0 259 175 305 734.8 0 305 176 233 734.8 0 233 177 190 734.8 0 190 178 188 734.8 0 188 179 210 734.8 0 210 180 237 734.8 0 237 181 269 734.8 0 269 182 159 734.8 0 159 183 148 734.8 0 148 184 182 734.8 0 182 185 186 734.8 0 186 186 151 734.8 0 151 187 102 734.8 0 102 188 122 734.8 0 122 189 260 734.8 0 260 190 378 734.8 0 378 191 323 734.8 0 323 192 215 734.8 0 215 193 189 734.8 0 189 194 107 734.8 0 107 195 189 734.8 0 189 196 291 734.8 0 291 197 459 734.8 0 459 198 345 734.8 0 345 199 104 734.8 0 104 200 165 734.8 0 165 201 179 734.8 0 179 202 363 734.8 0 363 203 365 734.8 0 365 204 168 734.8 0 168 205 159 734.8 0 159 206 189 734.8 0 189 207 166 734.8 0 166 208 115 734.8 0 115 209 93 734.8 0 93 210 162 734.8 0 162 211 108 734.8 0 108 212 155 734.8 0 155 213 148 734.8 0 148 214 165 734.8 0 165 215 213 734.8 0 213 216 352 734.8 0 352 217 1211 734.8 0 1211 218 462 734.8 0 462 219 187 734.8 0 187 220 134 734.8 0 134 221 157 734.8 0 157 222 183 734.8 0 183 223 229 734.8 0 229 224 174 734.8 0 174 225 236 734.8 0 236 226 251 734.8 0 251 227 286 734.8 0 286 228 393 734.8 0 393 229 2127 734.8 0 2127 230 1671 734.8 0 1671 231 491 734.8 0 491 232 143 734.8 0 143 233 165 734.8 0 165 234 164 734.8 0 164 235 202 734.8 0 202 236 165 734.8 0 165 237 121 734.8 0 121 238 110 734.8 0 110 239 124 734.8 0 124 240 92 734.8 0 92 241 81 734.8 0 81 242 79 734.8 0 79 243 110 734.8 0 110 244 142 734.8 0 142 245 208 734.8 0 208 246 262 734.8 0 262 247 167 734.8 0 167 248 78 734.8 0 78 249 32 734.8 0 32 250 80 734.8 0 80 251 210 734.8 0 210 252 298 734.8 0 298 253 163 734.8 0 163 254 139 734.8 0 139 255 117 734.8 0 117 256 148 734.8 0 148 257 116 734.8 0 116 258 131 734.8 0 131 259 352 734.8 0 352 260 400 734.8 0 400 261 228 734.8 0 228 262 211 734.8 0 211 263 197 734.8 0 197 264 182 734.8 0 182 265 189 734.8 0 189 266 302 734.8 0 302 267 230 734.8 0 230 268 520 734.8 0 520 269 153 734.8 0 153 270 147 734.8 0 147 271 277 734.8 0 277 272 175 734.8 0 175 273 124 734.8 0 124 274 126 734.8 0 126 275 154 734.8 0 154 276 212 734.8 0 212 277 177 734.8 0 177 278 193 734.8 0 193 279 242 734.8 0 242 280 95 734.8 0 95 281 101 734.8 0 101 282 109 734.8 0 109 283 247 734.8 0 247 284 321 734.8 0 321 285 235 734.8 0 235 286 285 734.8 0 285 287 512 734.8 0 512 288 491 734.8 0 491 289 219 734.8 0 219 290 258 734.8 0 258 291 322 734.8 0 322 292 731 734.8 0 731 293 15008 734.8 0 15008 294 10207 734.8 0 10207 295 2277 734.8 0 2277 296 86 734.8 0 86 297 78 734.8 0 78 298 127 734.8 0 127 299 182 734.8 0 182 300 177 734.8 0 177 301 324 734.8 0 324 Finished in 1.16 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 31,653 Reads with adapters: 8,195 (25.9%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 8,190 (25.9%) Total basepairs processed: 6,201,697 bp Total written (filtered): 2,258,255 bp (36.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8195 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 80 0.0 3 0 76 3 1 17 8038 0.0 3 7922 114 2 18 24 0.0 3 0 17 6 1 19 10 0.0 3 1 0 5 4 20 24 0.0 3 0 0 0 24 21 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.29 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 8,190 Reads with adapters: 151 (1.8%) Reads written (passing filters): 8,190 (100.0%) Total basepairs processed: 2,258,255 bp Total written (filtered): 2,257,802 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 151 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 87.4% C: 5.3% G: 6.6% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 151 128.0 0 151 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.