This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 78.77 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 99,056 (3.3%) Reads that were too short: 30,512 (1.0%) Reads written (passing filters): 68,544 (2.3%) Total basepairs processed: 905,907,359 bp Total written (filtered): 14,261,970 bp (1.6%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 99056 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1074 734.8 0 1074 7 19627 734.8 0 19627 8 2424 734.8 0 2424 9 3724 734.8 0 3724 10 36 734.8 0 36 11 22 734.8 0 22 12 17 734.8 0 17 13 23 734.8 0 23 14 26 734.8 0 26 15 15 734.8 0 15 16 22 734.8 0 22 17 17 734.8 0 17 18 12 734.8 0 12 19 29 734.8 0 29 20 22 734.8 0 22 21 10 734.8 0 10 22 24 734.8 0 24 23 12 734.8 0 12 24 16 734.8 0 16 25 20 734.8 0 20 26 26 734.8 0 26 27 40 734.8 0 40 28 30 734.8 0 30 29 26 734.8 0 26 30 20 734.8 0 20 31 15 734.8 0 15 32 24 734.8 0 24 33 20 734.8 0 20 34 16 734.8 0 16 35 32 734.8 0 32 36 44 734.8 0 44 37 41 734.8 0 41 38 70 734.8 0 70 39 42 734.8 0 42 40 33 734.8 0 33 41 24 734.8 0 24 42 78 734.8 0 78 43 22 734.8 0 22 44 16 734.8 0 16 45 20 734.8 0 20 46 26 734.8 0 26 47 25 734.8 0 25 48 13 734.8 0 13 49 21 734.8 0 21 50 25 734.8 0 25 51 35 734.8 0 35 52 21 734.8 0 21 53 210 734.8 0 210 54 213 734.8 0 213 55 28 734.8 0 28 56 20 734.8 0 20 57 123 734.8 0 123 58 13 734.8 0 13 59 19 734.8 0 19 60 44 734.8 0 44 61 14 734.8 0 14 62 16 734.8 0 16 63 19 734.8 0 19 64 19 734.8 0 19 65 17 734.8 0 17 66 46 734.8 0 46 67 37 734.8 0 37 68 17 734.8 0 17 69 28 734.8 0 28 70 32 734.8 0 32 71 82 734.8 0 82 72 25 734.8 0 25 73 23 734.8 0 23 74 23 734.8 0 23 75 17 734.8 0 17 76 26 734.8 0 26 77 27 734.8 0 27 78 37 734.8 0 37 79 44 734.8 0 44 80 37 734.8 0 37 81 41 734.8 0 41 82 86 734.8 0 86 83 47 734.8 0 47 84 42 734.8 0 42 85 84 734.8 0 84 86 70 734.8 0 70 87 86 734.8 0 86 88 88 734.8 0 88 89 118 734.8 0 118 90 181 734.8 0 181 91 155 734.8 0 155 92 111 734.8 0 111 93 224 734.8 0 224 94 197 734.8 0 197 95 117 734.8 0 117 96 37 734.8 0 37 97 37 734.8 0 37 98 51 734.8 0 51 99 37 734.8 0 37 100 25 734.8 0 25 101 108 734.8 0 108 102 102 734.8 0 102 103 145 734.8 0 145 104 173 734.8 0 173 105 151 734.8 0 151 106 92 734.8 0 92 107 169 734.8 0 169 108 72 734.8 0 72 109 75 734.8 0 75 110 70 734.8 0 70 111 324 734.8 0 324 112 253 734.8 0 253 113 321 734.8 0 321 114 243 734.8 0 243 115 161 734.8 0 161 116 77 734.8 0 77 117 72 734.8 0 72 118 59 734.8 0 59 119 95 734.8 0 95 120 115 734.8 0 115 121 102 734.8 0 102 122 81 734.8 0 81 123 130 734.8 0 130 124 193 734.8 0 193 125 804 734.8 0 804 126 885 734.8 0 885 127 464 734.8 0 464 128 100 734.8 0 100 129 140 734.8 0 140 130 185 734.8 0 185 131 143 734.8 0 143 132 138 734.8 0 138 133 187 734.8 0 187 134 270 734.8 0 270 135 145 734.8 0 145 136 167 734.8 0 167 137 194 734.8 0 194 138 234 734.8 0 234 139 303 734.8 0 303 140 198 734.8 0 198 141 167 734.8 0 167 142 128 734.8 0 128 143 186 734.8 0 186 144 331 734.8 0 331 145 422 734.8 0 422 146 340 734.8 0 340 147 246 734.8 0 246 148 172 734.8 0 172 149 208 734.8 0 208 150 436 734.8 0 436 151 669 734.8 0 669 152 398 734.8 0 398 153 258 734.8 0 258 154 347 734.8 0 347 155 817 734.8 0 817 156 461 734.8 0 461 157 430 734.8 0 430 158 499 734.8 0 499 159 598 734.8 0 598 160 4157 734.8 0 4157 161 4831 734.8 0 4831 162 1541 734.8 0 1541 163 292 734.8 0 292 164 451 734.8 0 451 165 442 734.8 0 442 166 284 734.8 0 284 167 168 734.8 0 168 168 227 734.8 0 227 169 240 734.8 0 240 170 237 734.8 0 237 171 195 734.8 0 195 172 178 734.8 0 178 173 214 734.8 0 214 174 204 734.8 0 204 175 222 734.8 0 222 176 278 734.8 0 278 177 272 734.8 0 272 178 288 734.8 0 288 179 269 734.8 0 269 180 1032 734.8 0 1032 181 1507 734.8 0 1507 182 1049 734.8 0 1049 183 261 734.8 0 261 184 199 734.8 0 199 185 267 734.8 0 267 186 343 734.8 0 343 187 228 734.8 0 228 188 168 734.8 0 168 189 271 734.8 0 271 190 267 734.8 0 267 191 271 734.8 0 271 192 158 734.8 0 158 193 137 734.8 0 137 194 81 734.8 0 81 195 121 734.8 0 121 196 149 734.8 0 149 197 154 734.8 0 154 198 143 734.8 0 143 199 164 734.8 0 164 200 190 734.8 0 190 201 274 734.8 0 274 202 174 734.8 0 174 203 115 734.8 0 115 204 155 734.8 0 155 205 272 734.8 0 272 206 221 734.8 0 221 207 208 734.8 0 208 208 215 734.8 0 215 209 188 734.8 0 188 210 188 734.8 0 188 211 232 734.8 0 232 212 900 734.8 0 900 213 1030 734.8 0 1030 214 372 734.8 0 372 215 239 734.8 0 239 216 355 734.8 0 355 217 329 734.8 0 329 218 283 734.8 0 283 219 176 734.8 0 176 220 242 734.8 0 242 221 248 734.8 0 248 222 284 734.8 0 284 223 217 734.8 0 217 224 284 734.8 0 284 225 396 734.8 0 396 226 320 734.8 0 320 227 260 734.8 0 260 228 210 734.8 0 210 229 259 734.8 0 259 230 233 734.8 0 233 231 222 734.8 0 222 232 168 734.8 0 168 233 187 734.8 0 187 234 207 734.8 0 207 235 156 734.8 0 156 236 178 734.8 0 178 237 240 734.8 0 240 238 268 734.8 0 268 239 236 734.8 0 236 240 263 734.8 0 263 241 405 734.8 0 405 242 292 734.8 0 292 243 321 734.8 0 321 244 293 734.8 0 293 245 349 734.8 0 349 246 309 734.8 0 309 247 325 734.8 0 325 248 226 734.8 0 226 249 160 734.8 0 160 250 267 734.8 0 267 251 235 734.8 0 235 252 163 734.8 0 163 253 176 734.8 0 176 254 455 734.8 0 455 255 495 734.8 0 495 256 889 734.8 0 889 257 613 734.8 0 613 258 264 734.8 0 264 259 163 734.8 0 163 260 207 734.8 0 207 261 189 734.8 0 189 262 201 734.8 0 201 263 167 734.8 0 167 264 230 734.8 0 230 265 238 734.8 0 238 266 662 734.8 0 662 267 487 734.8 0 487 268 354 734.8 0 354 269 235 734.8 0 235 270 298 734.8 0 298 271 418 734.8 0 418 272 823 734.8 0 823 273 2159 734.8 0 2159 274 722 734.8 0 722 275 289 734.8 0 289 276 361 734.8 0 361 277 467 734.8 0 467 278 240 734.8 0 240 279 166 734.8 0 166 280 228 734.8 0 228 281 326 734.8 0 326 282 518 734.8 0 518 283 344 734.8 0 344 284 284 734.8 0 284 285 229 734.8 0 229 286 125 734.8 0 125 287 292 734.8 0 292 288 438 734.8 0 438 289 301 734.8 0 301 290 131 734.8 0 131 291 176 734.8 0 176 292 111 734.8 0 111 293 119 734.8 0 119 294 128 734.8 0 128 295 88 734.8 0 88 296 101 734.8 0 101 297 85 734.8 0 85 298 164 734.8 0 164 299 105 734.8 0 105 300 81 734.8 0 81 301 95 734.8 0 95 Finished in 2.90 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 68,544 Reads with adapters: 20,687 (30.2%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 20,655 (30.1%) Total basepairs processed: 14,261,970 bp Total written (filtered): 5,652,559 bp (39.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 20687 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 3 18 22 0.0 3 0 0 15 2 5 19 353 0.0 3 0 338 8 3 4 20 19882 0.0 4 19421 385 46 23 7 21 302 0.0 4 255 43 2 1 1 22 4 0.0 4 1 1 0 1 1 23 19 0.0 4 1 1 0 12 5 24 1 0.0 4 0 0 0 0 1 25 2 0.0 4 0 0 0 0 2 30 1 0.0 4 1 35 2 0.0 4 0 0 0 0 2 38 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 66 2 0.0 4 0 0 0 0 2 70 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 86 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 0 0 1 97 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 1 110 2 0.0 4 0 0 0 2 111 35 0.0 4 0 0 1 30 4 112 5 0.0 4 0 0 0 4 1 113 1 0.0 4 0 1 115 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 127 12 0.0 4 0 0 0 0 12 128 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 251 2 0.0 4 0 0 0 0 2 252 4 0.0 4 0 0 0 0 4 253 3 0.0 4 0 0 0 0 3 255 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.70 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 20,655 Reads with adapters: 770 (3.7%) Reads written (passing filters): 20,655 (100.0%) Total basepairs processed: 5,652,559 bp Total written (filtered): 5,633,765 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 770 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.2% C: 3.0% G: 86.9% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 322.7 0 12 4 1 80.7 0 1 10 1 0.0 1 0 1 11 677 0.0 1 1 676 12 35 0.0 1 1 34 210 11 0.0 1 9 2 211 1 0.0 1 0 1 259 1 0.0 1 1 260 2 0.0 1 2 261 22 0.0 1 22 262 6 0.0 1 6 263 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.