This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 77.87 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 3,009,659 Reads with adapters: 465,608 (15.5%) Reads that were too short: 181,091 (6.0%) Reads written (passing filters): 284,517 (9.5%) Total basepairs processed: 905,907,359 bp Total written (filtered): 51,159,374 bp (5.6%) === Adapter 1 === Sequence: AACAAC; Type: regular 5'; Length: 6; Trimmed: 465608 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 255 734.8 0 255 7 22529 734.8 0 22529 8 78 734.8 0 78 9 49 734.8 0 49 10 31 734.8 0 31 11 45 734.8 0 45 12 49 734.8 0 49 13 38 734.8 0 38 14 47 734.8 0 47 15 43 734.8 0 43 16 45 734.8 0 45 17 30 734.8 0 30 18 39 734.8 0 39 19 46 734.8 0 46 20 29 734.8 0 29 21 47 734.8 0 47 22 49 734.8 0 49 23 53 734.8 0 53 24 48 734.8 0 48 25 44 734.8 0 44 26 37 734.8 0 37 27 40 734.8 0 40 28 37 734.8 0 37 29 43 734.8 0 43 30 60 734.8 0 60 31 56 734.8 0 56 32 31 734.8 0 31 33 39 734.8 0 39 34 49 734.8 0 49 35 33 734.8 0 33 36 47 734.8 0 47 37 38 734.8 0 38 38 44 734.8 0 44 39 50 734.8 0 50 40 194 734.8 0 194 41 3757 734.8 0 3757 42 36 734.8 0 36 43 57 734.8 0 57 44 42 734.8 0 42 45 42 734.8 0 42 46 42 734.8 0 42 47 48 734.8 0 48 48 47 734.8 0 47 49 48 734.8 0 48 50 32 734.8 0 32 51 27 734.8 0 27 52 45 734.8 0 45 53 39 734.8 0 39 54 35 734.8 0 35 55 35 734.8 0 35 56 36 734.8 0 36 57 33 734.8 0 33 58 51 734.8 0 51 59 44 734.8 0 44 60 68 734.8 0 68 61 51 734.8 0 51 62 41 734.8 0 41 63 39 734.8 0 39 64 46 734.8 0 46 65 149 734.8 0 149 66 108 734.8 0 108 67 52 734.8 0 52 68 62 734.8 0 62 69 55 734.8 0 55 70 71 734.8 0 71 71 101 734.8 0 101 72 77 734.8 0 77 73 107 734.8 0 107 74 105 734.8 0 105 75 95 734.8 0 95 76 574 734.8 0 574 77 1945 734.8 0 1945 78 2086 734.8 0 2086 79 2684 734.8 0 2684 80 2570 734.8 0 2570 81 3179 734.8 0 3179 82 3183 734.8 0 3183 83 3694 734.8 0 3694 84 3085 734.8 0 3085 85 2260 734.8 0 2260 86 2591 734.8 0 2591 87 3024 734.8 0 3024 88 1900 734.8 0 1900 89 3585 734.8 0 3585 90 3634 734.8 0 3634 91 3462 734.8 0 3462 92 4394 734.8 0 4394 93 2504 734.8 0 2504 94 2172 734.8 0 2172 95 1254 734.8 0 1254 96 905 734.8 0 905 97 1097 734.8 0 1097 98 893 734.8 0 893 99 849 734.8 0 849 100 1749 734.8 0 1749 101 1801 734.8 0 1801 102 1356 734.8 0 1356 103 2107 734.8 0 2107 104 2802 734.8 0 2802 105 4238 734.8 0 4238 106 1953 734.8 0 1953 107 2159 734.8 0 2159 108 2858 734.8 0 2858 109 2162 734.8 0 2162 110 2764 734.8 0 2764 111 2424 734.8 0 2424 112 2157 734.8 0 2157 113 1807 734.8 0 1807 114 2326 734.8 0 2326 115 2216 734.8 0 2216 116 1945 734.8 0 1945 117 2027 734.8 0 2027 118 1933 734.8 0 1933 119 1736 734.8 0 1736 120 2274 734.8 0 2274 121 2441 734.8 0 2441 122 2156 734.8 0 2156 123 2058 734.8 0 2058 124 2416 734.8 0 2416 125 2581 734.8 0 2581 126 2363 734.8 0 2363 127 1896 734.8 0 1896 128 1991 734.8 0 1991 129 1867 734.8 0 1867 130 1687 734.8 0 1687 131 1908 734.8 0 1908 132 2099 734.8 0 2099 133 1802 734.8 0 1802 134 1626 734.8 0 1626 135 1493 734.8 0 1493 136 1571 734.8 0 1571 137 1625 734.8 0 1625 138 1532 734.8 0 1532 139 1388 734.8 0 1388 140 1712 734.8 0 1712 141 1892 734.8 0 1892 142 1888 734.8 0 1888 143 1849 734.8 0 1849 144 1817 734.8 0 1817 145 2141 734.8 0 2141 146 2074 734.8 0 2074 147 2047 734.8 0 2047 148 2030 734.8 0 2030 149 2448 734.8 0 2448 150 2625 734.8 0 2625 151 2281 734.8 0 2281 152 2455 734.8 0 2455 153 2399 734.8 0 2399 154 2261 734.8 0 2261 155 1827 734.8 0 1827 156 1780 734.8 0 1780 157 1848 734.8 0 1848 158 1659 734.8 0 1659 159 1712 734.8 0 1712 160 1873 734.8 0 1873 161 2289 734.8 0 2289 162 2359 734.8 0 2359 163 1968 734.8 0 1968 164 1533 734.8 0 1533 165 1558 734.8 0 1558 166 1590 734.8 0 1590 167 1428 734.8 0 1428 168 1644 734.8 0 1644 169 1927 734.8 0 1927 170 2155 734.8 0 2155 171 2353 734.8 0 2353 172 1970 734.8 0 1970 173 1866 734.8 0 1866 174 2152 734.8 0 2152 175 1673 734.8 0 1673 176 1466 734.8 0 1466 177 1956 734.8 0 1956 178 2004 734.8 0 2004 179 1919 734.8 0 1919 180 1770 734.8 0 1770 181 1850 734.8 0 1850 182 2046 734.8 0 2046 183 1812 734.8 0 1812 184 1359 734.8 0 1359 185 1260 734.8 0 1260 186 1465 734.8 0 1465 187 1702 734.8 0 1702 188 1708 734.8 0 1708 189 1669 734.8 0 1669 190 1846 734.8 0 1846 191 1754 734.8 0 1754 192 1534 734.8 0 1534 193 1273 734.8 0 1273 194 1888 734.8 0 1888 195 1566 734.8 0 1566 196 1142 734.8 0 1142 197 1526 734.8 0 1526 198 1425 734.8 0 1425 199 1693 734.8 0 1693 200 1660 734.8 0 1660 201 1208 734.8 0 1208 202 1366 734.8 0 1366 203 1590 734.8 0 1590 204 1462 734.8 0 1462 205 1161 734.8 0 1161 206 1041 734.8 0 1041 207 1169 734.8 0 1169 208 1187 734.8 0 1187 209 1126 734.8 0 1126 210 1062 734.8 0 1062 211 1210 734.8 0 1210 212 1444 734.8 0 1444 213 1825 734.8 0 1825 214 1862 734.8 0 1862 215 1666 734.8 0 1666 216 1489 734.8 0 1489 217 1608 734.8 0 1608 218 1784 734.8 0 1784 219 1396 734.8 0 1396 220 1518 734.8 0 1518 221 1380 734.8 0 1380 222 1237 734.8 0 1237 223 1558 734.8 0 1558 224 1583 734.8 0 1583 225 1962 734.8 0 1962 226 3203 734.8 0 3203 227 3154 734.8 0 3154 228 2310 734.8 0 2310 229 2619 734.8 0 2619 230 6149 734.8 0 6149 231 4410 734.8 0 4410 232 2027 734.8 0 2027 233 1391 734.8 0 1391 234 1328 734.8 0 1328 235 1236 734.8 0 1236 236 1164 734.8 0 1164 237 1107 734.8 0 1107 238 960 734.8 0 960 239 1345 734.8 0 1345 240 1315 734.8 0 1315 241 1235 734.8 0 1235 242 1059 734.8 0 1059 243 1358 734.8 0 1358 244 1359 734.8 0 1359 245 1054 734.8 0 1054 246 1067 734.8 0 1067 247 1294 734.8 0 1294 248 1573 734.8 0 1573 249 1737 734.8 0 1737 250 1690 734.8 0 1690 251 1641 734.8 0 1641 252 2093 734.8 0 2093 253 1991 734.8 0 1991 254 1295 734.8 0 1295 255 956 734.8 0 956 256 1067 734.8 0 1067 257 1077 734.8 0 1077 258 1142 734.8 0 1142 259 1236 734.8 0 1236 260 1331 734.8 0 1331 261 1382 734.8 0 1382 262 1753 734.8 0 1753 263 3338 734.8 0 3338 264 8108 734.8 0 8108 265 17519 734.8 0 17519 266 3858 734.8 0 3858 267 1444 734.8 0 1444 268 1971 734.8 0 1971 269 1720 734.8 0 1720 270 1239 734.8 0 1239 271 1432 734.8 0 1432 272 1802 734.8 0 1802 273 1361 734.8 0 1361 274 1254 734.8 0 1254 275 1554 734.8 0 1554 276 1568 734.8 0 1568 277 1320 734.8 0 1320 278 1412 734.8 0 1412 279 1330 734.8 0 1330 280 1755 734.8 0 1755 281 1425 734.8 0 1425 282 855 734.8 0 855 283 1533 734.8 0 1533 284 1527 734.8 0 1527 285 1057 734.8 0 1057 286 978 734.8 0 978 287 1237 734.8 0 1237 288 1706 734.8 0 1706 289 1383 734.8 0 1383 290 1648 734.8 0 1648 291 2114 734.8 0 2114 292 1291 734.8 0 1291 293 1122 734.8 0 1122 294 1264 734.8 0 1264 295 1054 734.8 0 1054 296 1453 734.8 0 1453 297 1735 734.8 0 1735 298 1501 734.8 0 1501 299 1495 734.8 0 1495 300 1476 734.8 0 1476 301 458 734.8 0 458 Finished in 8.99 s (32 us/read; 1.90 M reads/minute). === Summary === Total reads processed: 284,517 Reads with adapters: 22,704 (8.0%) Reads that were too short: 52 (0.0%) Reads written (passing filters): 22,652 (8.0%) Total basepairs processed: 51,159,374 bp Total written (filtered): 6,262,449 bp (12.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22704 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 111 0.0 3 0 110 0 1 17 22333 0.0 3 22081 245 5 2 18 49 0.0 3 1 48 19 14 0.0 3 1 1 9 3 20 73 0.0 3 20 1 0 52 21 2 0.0 3 0 0 0 2 22 1 0.0 3 0 0 0 1 23 2 0.0 3 0 0 0 2 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 37 3 0.0 3 0 0 0 3 41 2 0.0 3 0 0 0 2 46 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 12 0.0 3 0 0 0 12 69 3 0.0 3 0 0 0 3 70 3 0.0 3 0 0 1 2 71 7 0.0 3 0 0 2 5 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 89 2 0.0 3 0 0 0 2 90 3 0.0 3 0 0 0 3 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 102 2 0.0 3 0 0 0 2 103 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 107 2 0.0 3 0 0 0 2 108 4 0.0 3 0 0 0 4 111 3 0.0 3 0 0 0 3 112 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 122 3 0.0 3 0 0 0 3 123 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 125 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 135 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 185 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 198 2 0.0 3 0 0 1 1 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.80 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 22,652 Reads with adapters: 632 (2.8%) Reads written (passing filters): 22,652 (100.0%) Total basepairs processed: 6,262,449 bp Total written (filtered): 6,260,552 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 632 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 23.7% C: 68.2% G: 7.8% T: 0.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 631 353.9 0 631 4 1 88.5 0 1