This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.41 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 131,665 (5.0%) Reads that were too short: 14,571 (0.6%) Reads written (passing filters): 117,094 (4.5%) Total basepairs processed: 791,719,999 bp Total written (filtered): 20,004,331 bp (2.5%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 131665 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 140 642.2 0 140 7 887 642.2 0 887 8 850 642.2 0 850 9 147 642.2 0 147 10 106 642.2 0 106 11 66 642.2 0 66 12 89 642.2 0 89 13 73 642.2 0 73 14 135 642.2 0 135 15 109 642.2 0 109 16 79 642.2 0 79 17 138 642.2 0 138 18 113 642.2 0 113 19 81 642.2 0 81 20 98 642.2 0 98 21 103 642.2 0 103 22 101 642.2 0 101 23 88 642.2 0 88 24 81 642.2 0 81 25 89 642.2 0 89 26 113 642.2 0 113 27 217 642.2 0 217 28 377 642.2 0 377 29 333 642.2 0 333 30 94 642.2 0 94 31 89 642.2 0 89 32 117 642.2 0 117 33 117 642.2 0 117 34 125 642.2 0 125 35 521 642.2 0 521 36 506 642.2 0 506 37 101 642.2 0 101 38 107 642.2 0 107 39 94 642.2 0 94 40 74 642.2 0 74 41 78 642.2 0 78 42 96 642.2 0 96 43 81 642.2 0 81 44 92 642.2 0 92 45 134 642.2 0 134 46 149 642.2 0 149 47 89 642.2 0 89 48 102 642.2 0 102 49 83 642.2 0 83 50 133 642.2 0 133 51 168 642.2 0 168 52 149 642.2 0 149 53 92 642.2 0 92 54 113 642.2 0 113 55 100 642.2 0 100 56 109 642.2 0 109 57 132 642.2 0 132 58 441 642.2 0 441 59 479 642.2 0 479 60 413 642.2 0 413 61 90 642.2 0 90 62 107 642.2 0 107 63 102 642.2 0 102 64 78 642.2 0 78 65 85 642.2 0 85 66 98 642.2 0 98 67 78 642.2 0 78 68 117 642.2 0 117 69 225 642.2 0 225 70 357 642.2 0 357 71 237 642.2 0 237 72 1568 642.2 0 1568 73 1079 642.2 0 1079 74 1293 642.2 0 1293 75 83 642.2 0 83 76 86 642.2 0 86 77 103 642.2 0 103 78 86 642.2 0 86 79 77 642.2 0 77 80 63 642.2 0 63 81 74 642.2 0 74 82 63 642.2 0 63 83 102 642.2 0 102 84 93 642.2 0 93 85 85 642.2 0 85 86 88 642.2 0 88 87 93 642.2 0 93 88 146 642.2 0 146 89 128 642.2 0 128 90 289 642.2 0 289 91 159 642.2 0 159 92 380 642.2 0 380 93 97 642.2 0 97 94 68 642.2 0 68 95 104 642.2 0 104 96 96 642.2 0 96 97 89 642.2 0 89 98 136 642.2 0 136 99 87 642.2 0 87 100 74 642.2 0 74 101 101 642.2 0 101 102 95 642.2 0 95 103 142 642.2 0 142 104 110 642.2 0 110 105 64 642.2 0 64 106 78 642.2 0 78 107 110 642.2 0 110 108 119 642.2 0 119 109 124 642.2 0 124 110 140 642.2 0 140 111 81 642.2 0 81 112 184 642.2 0 184 113 354 642.2 0 354 114 564 642.2 0 564 115 74 642.2 0 74 116 89 642.2 0 89 117 128 642.2 0 128 118 85 642.2 0 85 119 178 642.2 0 178 120 87 642.2 0 87 121 140 642.2 0 140 122 83 642.2 0 83 123 97 642.2 0 97 124 80 642.2 0 80 125 85 642.2 0 85 126 95 642.2 0 95 127 98 642.2 0 98 128 111 642.2 0 111 129 234 642.2 0 234 130 357 642.2 0 357 131 138 642.2 0 138 132 142 642.2 0 142 133 205 642.2 0 205 134 148 642.2 0 148 135 160 642.2 0 160 136 247 642.2 0 247 137 216 642.2 0 216 138 232 642.2 0 232 139 467 642.2 0 467 140 494 642.2 0 494 141 652 642.2 0 652 142 1111 642.2 0 1111 143 10223 642.2 0 10223 144 67829 642.2 0 67829 145 4795 642.2 0 4795 146 141 642.2 0 141 147 113 642.2 0 113 148 171 642.2 0 171 149 455 642.2 0 455 150 116 642.2 0 116 151 65 642.2 0 65 152 111 642.2 0 111 153 64 642.2 0 64 154 85 642.2 0 85 155 261 642.2 0 261 156 276 642.2 0 276 157 78 642.2 0 78 158 105 642.2 0 105 159 95 642.2 0 95 160 96 642.2 0 96 161 96 642.2 0 96 162 139 642.2 0 139 163 1400 642.2 0 1400 164 184 642.2 0 184 165 125 642.2 0 125 166 76 642.2 0 76 167 95 642.2 0 95 168 108 642.2 0 108 169 82 642.2 0 82 170 87 642.2 0 87 171 102 642.2 0 102 172 77 642.2 0 77 173 100 642.2 0 100 174 91 642.2 0 91 175 64 642.2 0 64 176 105 642.2 0 105 177 86 642.2 0 86 178 86 642.2 0 86 179 89 642.2 0 89 180 82 642.2 0 82 181 71 642.2 0 71 182 86 642.2 0 86 183 101 642.2 0 101 184 95 642.2 0 95 185 100 642.2 0 100 186 92 642.2 0 92 187 109 642.2 0 109 188 143 642.2 0 143 189 90 642.2 0 90 190 78 642.2 0 78 191 75 642.2 0 75 192 82 642.2 0 82 193 82 642.2 0 82 194 95 642.2 0 95 195 89 642.2 0 89 196 126 642.2 0 126 197 90 642.2 0 90 198 124 642.2 0 124 199 75 642.2 0 75 200 98 642.2 0 98 201 87 642.2 0 87 202 74 642.2 0 74 203 118 642.2 0 118 204 105 642.2 0 105 205 102 642.2 0 102 206 114 642.2 0 114 207 103 642.2 0 103 208 133 642.2 0 133 209 145 642.2 0 145 210 152 642.2 0 152 211 181 642.2 0 181 212 135 642.2 0 135 213 230 642.2 0 230 214 196 642.2 0 196 215 865 642.2 0 865 216 670 642.2 0 670 217 722 642.2 0 722 218 89 642.2 0 89 219 77 642.2 0 77 220 94 642.2 0 94 221 129 642.2 0 129 222 130 642.2 0 130 223 118 642.2 0 118 224 138 642.2 0 138 225 152 642.2 0 152 226 126 642.2 0 126 227 105 642.2 0 105 228 157 642.2 0 157 229 85 642.2 0 85 230 104 642.2 0 104 231 206 642.2 0 206 232 336 642.2 0 336 233 331 642.2 0 331 234 180 642.2 0 180 235 149 642.2 0 149 236 161 642.2 0 161 237 103 642.2 0 103 238 67 642.2 0 67 239 75 642.2 0 75 240 78 642.2 0 78 241 86 642.2 0 86 242 91 642.2 0 91 243 97 642.2 0 97 244 126 642.2 0 126 245 136 642.2 0 136 246 97 642.2 0 97 247 73 642.2 0 73 248 73 642.2 0 73 249 69 642.2 0 69 250 48 642.2 0 48 251 83 642.2 0 83 252 77 642.2 0 77 253 126 642.2 0 126 254 131 642.2 0 131 255 131 642.2 0 131 256 99 642.2 0 99 257 105 642.2 0 105 258 119 642.2 0 119 259 104 642.2 0 104 260 125 642.2 0 125 261 93 642.2 0 93 262 78 642.2 0 78 263 169 642.2 0 169 264 418 642.2 0 418 265 181 642.2 0 181 266 222 642.2 0 222 267 95 642.2 0 95 268 67 642.2 0 67 269 108 642.2 0 108 270 120 642.2 0 120 271 112 642.2 0 112 272 99 642.2 0 99 273 110 642.2 0 110 274 91 642.2 0 91 275 97 642.2 0 97 276 94 642.2 0 94 277 113 642.2 0 113 278 103 642.2 0 103 279 122 642.2 0 122 280 109 642.2 0 109 281 122 642.2 0 122 282 127 642.2 0 127 283 156 642.2 0 156 284 151 642.2 0 151 285 170 642.2 0 170 286 141 642.2 0 141 287 104 642.2 0 104 288 95 642.2 0 95 289 95 642.2 0 95 290 113 642.2 0 113 291 98 642.2 0 98 292 113 642.2 0 113 293 135 642.2 0 135 294 115 642.2 0 115 295 166 642.2 0 166 296 187 642.2 0 187 297 233 642.2 0 233 298 123 642.2 0 123 299 117 642.2 0 117 300 99 642.2 0 99 301 79 642.2 0 79 Finished in 4.40 s (38 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 117,094 Reads with adapters: 887 (0.8%) Reads that were too short: 83 (0.1%) Reads written (passing filters): 804 (0.7%) Total basepairs processed: 20,004,331 bp Total written (filtered): 217,962 bp (1.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 887 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 6 0.0 3 0 6 20 775 0.0 4 703 60 10 1 1 21 7 0.0 4 3 1 3 22 1 0.0 4 0 0 0 0 1 23 3 0.0 4 0 0 0 2 1 42 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 50 1 0.0 4 1 52 1 0.0 4 1 60 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 75 1 0.0 4 1 78 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 89 5 0.0 4 0 0 0 0 5 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 98 8 0.0 4 0 0 0 0 8 99 2 0.0 4 0 0 0 0 2 102 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 1 109 1 0.0 4 0 0 0 0 1 119 2 0.0 4 0 0 0 0 2 120 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 129 3 0.0 4 0 0 0 0 3 133 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 146 34 0.0 4 0 0 0 0 34 147 5 0.0 4 0 0 0 0 5 148 1 0.0 4 0 0 0 0 1 149 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 1 157 1 0.0 4 0 0 0 0 1 160 3 0.0 4 0 0 0 0 3 161 1 0.0 4 0 0 0 1 162 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.04 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 804 Reads with adapters: 299 (37.2%) Reads written (passing filters): 804 (100.0%) Total basepairs processed: 217,962 bp Total written (filtered): 169,079 bp (77.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 299 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.0% C: 40.1% G: 54.8% T: 3.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 12.6 0 2 10 15 0.0 1 3 12 11 105 0.0 1 1 104 13 1 0.0 1 0 1 269 1 0.0 1 1 270 142 0.0 1 132 10 271 26 0.0 1 24 2 272 7 0.0 1 6 1