This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.29 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 135,672 (5.2%) Reads that were too short: 14,008 (0.5%) Reads written (passing filters): 121,664 (4.6%) Total basepairs processed: 791,719,999 bp Total written (filtered): 20,695,822 bp (2.6%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 135672 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 147 642.2 0 147 7 992 642.2 0 992 8 867 642.2 0 867 9 113 642.2 0 113 10 94 642.2 0 94 11 103 642.2 0 103 12 97 642.2 0 97 13 88 642.2 0 88 14 116 642.2 0 116 15 111 642.2 0 111 16 102 642.2 0 102 17 108 642.2 0 108 18 100 642.2 0 100 19 97 642.2 0 97 20 129 642.2 0 129 21 105 642.2 0 105 22 108 642.2 0 108 23 106 642.2 0 106 24 94 642.2 0 94 25 84 642.2 0 84 26 162 642.2 0 162 27 115 642.2 0 115 28 146 642.2 0 146 29 125 642.2 0 125 30 114 642.2 0 114 31 106 642.2 0 106 32 148 642.2 0 148 33 132 642.2 0 132 34 139 642.2 0 139 35 498 642.2 0 498 36 499 642.2 0 499 37 97 642.2 0 97 38 112 642.2 0 112 39 123 642.2 0 123 40 104 642.2 0 104 41 106 642.2 0 106 42 129 642.2 0 129 43 87 642.2 0 87 44 88 642.2 0 88 45 120 642.2 0 120 46 117 642.2 0 117 47 115 642.2 0 115 48 96 642.2 0 96 49 131 642.2 0 131 50 137 642.2 0 137 51 123 642.2 0 123 52 160 642.2 0 160 53 149 642.2 0 149 54 143 642.2 0 143 55 119 642.2 0 119 56 148 642.2 0 148 57 133 642.2 0 133 58 405 642.2 0 405 59 514 642.2 0 514 60 451 642.2 0 451 61 108 642.2 0 108 62 158 642.2 0 158 63 116 642.2 0 116 64 105 642.2 0 105 65 95 642.2 0 95 66 93 642.2 0 93 67 89 642.2 0 89 68 120 642.2 0 120 69 145 642.2 0 145 70 198 642.2 0 198 71 208 642.2 0 208 72 1321 642.2 0 1321 73 1002 642.2 0 1002 74 1413 642.2 0 1413 75 81 642.2 0 81 76 109 642.2 0 109 77 121 642.2 0 121 78 124 642.2 0 124 79 101 642.2 0 101 80 94 642.2 0 94 81 87 642.2 0 87 82 83 642.2 0 83 83 113 642.2 0 113 84 102 642.2 0 102 85 109 642.2 0 109 86 92 642.2 0 92 87 112 642.2 0 112 88 142 642.2 0 142 89 133 642.2 0 133 90 223 642.2 0 223 91 127 642.2 0 127 92 285 642.2 0 285 93 164 642.2 0 164 94 97 642.2 0 97 95 102 642.2 0 102 96 168 642.2 0 168 97 94 642.2 0 94 98 119 642.2 0 119 99 133 642.2 0 133 100 111 642.2 0 111 101 111 642.2 0 111 102 111 642.2 0 111 103 143 642.2 0 143 104 131 642.2 0 131 105 101 642.2 0 101 106 120 642.2 0 120 107 136 642.2 0 136 108 116 642.2 0 116 109 137 642.2 0 137 110 114 642.2 0 114 111 96 642.2 0 96 112 117 642.2 0 117 113 238 642.2 0 238 114 200 642.2 0 200 115 91 642.2 0 91 116 102 642.2 0 102 117 132 642.2 0 132 118 92 642.2 0 92 119 213 642.2 0 213 120 141 642.2 0 141 121 133 642.2 0 133 122 119 642.2 0 119 123 142 642.2 0 142 124 103 642.2 0 103 125 122 642.2 0 122 126 122 642.2 0 122 127 134 642.2 0 134 128 127 642.2 0 127 129 191 642.2 0 191 130 367 642.2 0 367 131 166 642.2 0 166 132 147 642.2 0 147 133 234 642.2 0 234 134 192 642.2 0 192 135 183 642.2 0 183 136 304 642.2 0 304 137 260 642.2 0 260 138 230 642.2 0 230 139 492 642.2 0 492 140 535 642.2 0 535 141 484 642.2 0 484 142 931 642.2 0 931 143 9012 642.2 0 9012 144 73103 642.2 0 73103 145 5113 642.2 0 5113 146 128 642.2 0 128 147 114 642.2 0 114 148 141 642.2 0 141 149 170 642.2 0 170 150 118 642.2 0 118 151 106 642.2 0 106 152 123 642.2 0 123 153 81 642.2 0 81 154 90 642.2 0 90 155 295 642.2 0 295 156 353 642.2 0 353 157 97 642.2 0 97 158 89 642.2 0 89 159 134 642.2 0 134 160 97 642.2 0 97 161 126 642.2 0 126 162 155 642.2 0 155 163 1346 642.2 0 1346 164 154 642.2 0 154 165 145 642.2 0 145 166 97 642.2 0 97 167 121 642.2 0 121 168 109 642.2 0 109 169 98 642.2 0 98 170 135 642.2 0 135 171 119 642.2 0 119 172 83 642.2 0 83 173 94 642.2 0 94 174 88 642.2 0 88 175 107 642.2 0 107 176 110 642.2 0 110 177 108 642.2 0 108 178 95 642.2 0 95 179 102 642.2 0 102 180 118 642.2 0 118 181 94 642.2 0 94 182 88 642.2 0 88 183 100 642.2 0 100 184 89 642.2 0 89 185 100 642.2 0 100 186 106 642.2 0 106 187 96 642.2 0 96 188 136 642.2 0 136 189 114 642.2 0 114 190 105 642.2 0 105 191 98 642.2 0 98 192 82 642.2 0 82 193 118 642.2 0 118 194 85 642.2 0 85 195 96 642.2 0 96 196 131 642.2 0 131 197 96 642.2 0 96 198 119 642.2 0 119 199 89 642.2 0 89 200 111 642.2 0 111 201 128 642.2 0 128 202 100 642.2 0 100 203 121 642.2 0 121 204 121 642.2 0 121 205 108 642.2 0 108 206 133 642.2 0 133 207 116 642.2 0 116 208 97 642.2 0 97 209 173 642.2 0 173 210 108 642.2 0 108 211 99 642.2 0 99 212 172 642.2 0 172 213 161 642.2 0 161 214 162 642.2 0 162 215 896 642.2 0 896 216 815 642.2 0 815 217 955 642.2 0 955 218 87 642.2 0 87 219 104 642.2 0 104 220 142 642.2 0 142 221 96 642.2 0 96 222 97 642.2 0 97 223 107 642.2 0 107 224 112 642.2 0 112 225 99 642.2 0 99 226 115 642.2 0 115 227 117 642.2 0 117 228 122 642.2 0 122 229 80 642.2 0 80 230 108 642.2 0 108 231 112 642.2 0 112 232 96 642.2 0 96 233 138 642.2 0 138 234 132 642.2 0 132 235 98 642.2 0 98 236 112 642.2 0 112 237 98 642.2 0 98 238 73 642.2 0 73 239 114 642.2 0 114 240 101 642.2 0 101 241 98 642.2 0 98 242 107 642.2 0 107 243 93 642.2 0 93 244 132 642.2 0 132 245 159 642.2 0 159 246 109 642.2 0 109 247 85 642.2 0 85 248 101 642.2 0 101 249 127 642.2 0 127 250 91 642.2 0 91 251 107 642.2 0 107 252 119 642.2 0 119 253 127 642.2 0 127 254 161 642.2 0 161 255 138 642.2 0 138 256 100 642.2 0 100 257 124 642.2 0 124 258 112 642.2 0 112 259 128 642.2 0 128 260 142 642.2 0 142 261 119 642.2 0 119 262 82 642.2 0 82 263 141 642.2 0 141 264 173 642.2 0 173 265 126 642.2 0 126 266 134 642.2 0 134 267 108 642.2 0 108 268 104 642.2 0 104 269 134 642.2 0 134 270 104 642.2 0 104 271 118 642.2 0 118 272 120 642.2 0 120 273 115 642.2 0 115 274 145 642.2 0 145 275 105 642.2 0 105 276 125 642.2 0 125 277 83 642.2 0 83 278 126 642.2 0 126 279 101 642.2 0 101 280 125 642.2 0 125 281 104 642.2 0 104 282 131 642.2 0 131 283 129 642.2 0 129 284 146 642.2 0 146 285 121 642.2 0 121 286 98 642.2 0 98 287 141 642.2 0 141 288 109 642.2 0 109 289 107 642.2 0 107 290 92 642.2 0 92 291 118 642.2 0 118 292 117 642.2 0 117 293 94 642.2 0 94 294 124 642.2 0 124 295 116 642.2 0 116 296 143 642.2 0 143 297 146 642.2 0 146 298 116 642.2 0 116 299 128 642.2 0 128 300 98 642.2 0 98 301 85 642.2 0 85 Finished in 3.94 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 121,664 Reads with adapters: 964 (0.8%) Reads that were too short: 85 (0.1%) Reads written (passing filters): 879 (0.7%) Total basepairs processed: 20,695,822 bp Total written (filtered): 235,192 bp (1.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 964 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 4 0.0 3 0 4 17 819 0.0 3 805 14 18 3 0.0 3 0 3 20 1 0.0 3 1 28 12 0.0 3 0 0 0 12 32 1 0.0 3 0 0 0 1 48 20 0.0 3 0 0 0 20 50 1 0.0 3 1 51 6 0.0 3 0 0 2 4 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 99 6 0.0 3 0 0 0 6 106 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 1 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 135 39 0.0 3 0 0 1 38 139 1 0.0 3 0 0 0 1 143 1 0.0 3 1 149 3 0.0 3 1 1 0 1 150 25 0.0 3 0 0 2 23 151 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 879 Reads with adapters: 1 (0.1%) Reads written (passing filters): 879 (100.0%) Total basepairs processed: 235,192 bp Total written (filtered): 235,189 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 13.7 0 1