This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.44 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 179,304 (7.3%) Reads that were too short: 51,362 (2.1%) Reads written (passing filters): 127,942 (5.2%) Total basepairs processed: 735,190,694 bp Total written (filtered): 24,749,078 bp (3.4%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 179304 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 210 596.3 0 210 7 9182 596.3 0 9182 8 9234 596.3 0 9234 9 25 596.3 0 25 10 6 596.3 0 6 11 34 596.3 0 34 12 28 596.3 0 28 13 28 596.3 0 28 14 15 596.3 0 15 15 33 596.3 0 33 16 20 596.3 0 20 17 15 596.3 0 15 18 20 596.3 0 20 19 17 596.3 0 17 20 26 596.3 0 26 21 28 596.3 0 28 22 23 596.3 0 23 23 20 596.3 0 20 24 24 596.3 0 24 25 22 596.3 0 22 26 23 596.3 0 23 27 15 596.3 0 15 28 28 596.3 0 28 29 24 596.3 0 24 30 206 596.3 0 206 31 50 596.3 0 50 32 14 596.3 0 14 33 25 596.3 0 25 34 10 596.3 0 10 35 23 596.3 0 23 36 34 596.3 0 34 37 24 596.3 0 24 38 28 596.3 0 28 39 33 596.3 0 33 40 35 596.3 0 35 41 19 596.3 0 19 42 24 596.3 0 24 43 27 596.3 0 27 44 22 596.3 0 22 45 22 596.3 0 22 46 30 596.3 0 30 47 26 596.3 0 26 48 27 596.3 0 27 49 29 596.3 0 29 50 31 596.3 0 31 51 20 596.3 0 20 52 23 596.3 0 23 53 30 596.3 0 30 54 20 596.3 0 20 55 26 596.3 0 26 56 29 596.3 0 29 57 29 596.3 0 29 58 48 596.3 0 48 59 41 596.3 0 41 60 42 596.3 0 42 61 40 596.3 0 40 62 26 596.3 0 26 63 40 596.3 0 40 64 40 596.3 0 40 65 40 596.3 0 40 66 62 596.3 0 62 67 43 596.3 0 43 68 54 596.3 0 54 69 74 596.3 0 74 70 890 596.3 0 890 71 1046 596.3 0 1046 72 826 596.3 0 826 73 35 596.3 0 35 74 37 596.3 0 37 75 24 596.3 0 24 76 33 596.3 0 33 77 31 596.3 0 31 78 28 596.3 0 28 79 30 596.3 0 30 80 57 596.3 0 57 81 76 596.3 0 76 82 140 596.3 0 140 83 101 596.3 0 101 84 38 596.3 0 38 85 32 596.3 0 32 86 38 596.3 0 38 87 42 596.3 0 42 88 21 596.3 0 21 89 23 596.3 0 23 90 24 596.3 0 24 91 54 596.3 0 54 92 34 596.3 0 34 93 40 596.3 0 40 94 42 596.3 0 42 95 32 596.3 0 32 96 31 596.3 0 31 97 59 596.3 0 59 98 75 596.3 0 75 99 52 596.3 0 52 100 130 596.3 0 130 101 82 596.3 0 82 102 63 596.3 0 63 103 57 596.3 0 57 104 51 596.3 0 51 105 34 596.3 0 34 106 68 596.3 0 68 107 107 596.3 0 107 108 97 596.3 0 97 109 211 596.3 0 211 110 286 596.3 0 286 111 669 596.3 0 669 112 188 596.3 0 188 113 217 596.3 0 217 114 439 596.3 0 439 115 226 596.3 0 226 116 199 596.3 0 199 117 371 596.3 0 371 118 308 596.3 0 308 119 430 596.3 0 430 120 440 596.3 0 440 121 502 596.3 0 502 122 594 596.3 0 594 123 1035 596.3 0 1035 124 19209 596.3 0 19209 125 36692 596.3 0 36692 126 29375 596.3 0 29375 127 222 596.3 0 222 128 75 596.3 0 75 129 184 596.3 0 184 130 78 596.3 0 78 131 57 596.3 0 57 132 40 596.3 0 40 133 45 596.3 0 45 134 46 596.3 0 46 135 61 596.3 0 61 136 89 596.3 0 89 137 76 596.3 0 76 138 221 596.3 0 221 139 331 596.3 0 331 140 64 596.3 0 64 141 71 596.3 0 71 142 113 596.3 0 113 143 89 596.3 0 89 144 67 596.3 0 67 145 69 596.3 0 69 146 64 596.3 0 64 147 69 596.3 0 69 148 112 596.3 0 112 149 1465 596.3 0 1465 150 1640 596.3 0 1640 151 1982 596.3 0 1982 152 98 596.3 0 98 153 25 596.3 0 25 154 46 596.3 0 46 155 47 596.3 0 47 156 37 596.3 0 37 157 41 596.3 0 41 158 65 596.3 0 65 159 65 596.3 0 65 160 31 596.3 0 31 161 48 596.3 0 48 162 51 596.3 0 51 163 161 596.3 0 161 164 315 596.3 0 315 165 43 596.3 0 43 166 49 596.3 0 49 167 40 596.3 0 40 168 34 596.3 0 34 169 51 596.3 0 51 170 38 596.3 0 38 171 43 596.3 0 43 172 33 596.3 0 33 173 36 596.3 0 36 174 19 596.3 0 19 175 34 596.3 0 34 176 31 596.3 0 31 177 42 596.3 0 42 178 40 596.3 0 40 179 38 596.3 0 38 180 42 596.3 0 42 181 126 596.3 0 126 182 247 596.3 0 247 183 470 596.3 0 470 184 47 596.3 0 47 185 71 596.3 0 71 186 56 596.3 0 56 187 48 596.3 0 48 188 118 596.3 0 118 189 338 596.3 0 338 190 309 596.3 0 309 191 35 596.3 0 35 192 22 596.3 0 22 193 28 596.3 0 28 194 44 596.3 0 44 195 52 596.3 0 52 196 47 596.3 0 47 197 52 596.3 0 52 198 93 596.3 0 93 199 47 596.3 0 47 200 38 596.3 0 38 201 44 596.3 0 44 202 24 596.3 0 24 203 55 596.3 0 55 204 69 596.3 0 69 205 51 596.3 0 51 206 59 596.3 0 59 207 102 596.3 0 102 208 201 596.3 0 201 209 78 596.3 0 78 210 105 596.3 0 105 211 68 596.3 0 68 212 165 596.3 0 165 213 313 596.3 0 313 214 252 596.3 0 252 215 86 596.3 0 86 216 53 596.3 0 53 217 65 596.3 0 65 218 103 596.3 0 103 219 90 596.3 0 90 220 179 596.3 0 179 221 248 596.3 0 248 222 337 596.3 0 337 223 927 596.3 0 927 224 1395 596.3 0 1395 225 14779 596.3 0 14779 226 9847 596.3 0 9847 227 18222 596.3 0 18222 228 105 596.3 0 105 229 37 596.3 0 37 230 38 596.3 0 38 231 35 596.3 0 35 232 44 596.3 0 44 233 38 596.3 0 38 234 35 596.3 0 35 235 64 596.3 0 64 236 85 596.3 0 85 237 52 596.3 0 52 238 47 596.3 0 47 239 44 596.3 0 44 240 51 596.3 0 51 241 43 596.3 0 43 242 43 596.3 0 43 243 65 596.3 0 65 244 77 596.3 0 77 245 65 596.3 0 65 246 41 596.3 0 41 247 38 596.3 0 38 248 34 596.3 0 34 249 43 596.3 0 43 250 31 596.3 0 31 251 48 596.3 0 48 252 49 596.3 0 49 253 60 596.3 0 60 254 67 596.3 0 67 255 49 596.3 0 49 256 82 596.3 0 82 257 57 596.3 0 57 258 62 596.3 0 62 259 50 596.3 0 50 260 64 596.3 0 64 261 44 596.3 0 44 262 52 596.3 0 52 263 72 596.3 0 72 264 51 596.3 0 51 265 50 596.3 0 50 266 71 596.3 0 71 267 71 596.3 0 71 268 75 596.3 0 75 269 56 596.3 0 56 270 44 596.3 0 44 271 31 596.3 0 31 272 28 596.3 0 28 273 45 596.3 0 45 274 51 596.3 0 51 275 45 596.3 0 45 276 37 596.3 0 37 277 30 596.3 0 30 278 33 596.3 0 33 279 32 596.3 0 32 280 45 596.3 0 45 281 52 596.3 0 52 282 38 596.3 0 38 283 36 596.3 0 36 284 43 596.3 0 43 285 36 596.3 0 36 286 28 596.3 0 28 287 47 596.3 0 47 288 43 596.3 0 43 289 44 596.3 0 44 290 24 596.3 0 24 291 31 596.3 0 31 292 37 596.3 0 37 293 34 596.3 0 34 294 31 596.3 0 31 295 44 596.3 0 44 296 35 596.3 0 35 297 27 596.3 0 27 298 50 596.3 0 50 299 33 596.3 0 33 300 36 596.3 0 36 301 34 596.3 0 34 Finished in 4.64 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 127,942 Reads with adapters: 9,987 (7.8%) Reads that were too short: 77 (0.1%) Reads written (passing filters): 9,910 (7.7%) Total basepairs processed: 24,749,078 bp Total written (filtered): 2,613,053 bp (10.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9987 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 29 0.0 3 0 23 6 17 5345 0.0 3 5217 125 2 1 18 3735 0.0 3 3657 75 3 19 6 0.0 3 0 4 1 1 20 12 0.0 3 0 0 2 10 21 4 0.0 3 0 0 0 4 24 1 0.0 3 0 0 0 1 26 5 0.0 3 0 0 1 4 28 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 97 0.0 3 0 0 1 96 45 34 0.0 3 0 0 1 33 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 2 0.0 3 0 0 0 2 62 2 0.0 3 0 1 0 1 65 1 0.0 3 0 0 0 1 66 13 0.0 3 0 0 0 13 67 2 0.0 3 0 0 0 2 68 596 0.0 3 0 0 0 596 69 2 0.0 3 0 0 0 2 70 1 0.0 3 1 71 1 0.0 3 1 72 1 0.0 3 1 73 3 0.0 3 3 74 1 0.0 3 1 75 2 0.0 3 2 79 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 93 3 0.0 3 0 0 0 3 102 9 0.0 3 0 0 0 9 110 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 142 5 0.0 3 0 0 0 5 143 5 0.0 3 0 0 0 5 144 3 0.0 3 0 0 0 3 151 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 218 18 0.0 3 0 0 0 18 221 8 0.0 3 0 0 0 8 232 1 0.0 3 1 269 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 287 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.35 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 9,910 Reads with adapters: 142 (1.4%) Reads written (passing filters): 9,910 (100.0%) Total basepairs processed: 2,613,053 bp Total written (filtered): 2,612,592 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 142 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 47.9% C: 21.1% G: 15.5% T: 15.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 115 154.8 0 115 4 21 38.7 0 21 5 4 9.7 0 4 6 2 2.4 0 2