This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 180.64 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 552,181 (15.2%) Reads that were too short: 29,321 (0.8%) Reads written (passing filters): 522,860 (14.4%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 77,312,619 bp (7.1%) === Adapter 1 === Sequence: CACTCT; Type: regular 5'; Length: 6; Trimmed: 552181 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 373 887.3 0 373 7 2005 887.3 0 2005 8 124 887.3 0 124 9 2275 887.3 0 2275 10 270 887.3 0 270 11 41 887.3 0 41 12 167 887.3 0 167 13 57 887.3 0 57 14 74 887.3 0 74 15 62 887.3 0 62 16 41 887.3 0 41 17 64 887.3 0 64 18 40 887.3 0 40 19 46 887.3 0 46 20 49 887.3 0 49 21 76 887.3 0 76 22 51 887.3 0 51 23 40 887.3 0 40 24 54 887.3 0 54 25 52 887.3 0 52 26 59 887.3 0 59 27 44 887.3 0 44 28 49 887.3 0 49 29 41 887.3 0 41 30 83 887.3 0 83 31 81 887.3 0 81 32 49 887.3 0 49 33 49 887.3 0 49 34 64 887.3 0 64 35 54 887.3 0 54 36 77 887.3 0 77 37 56 887.3 0 56 38 72 887.3 0 72 39 86 887.3 0 86 40 73 887.3 0 73 41 57 887.3 0 57 42 53 887.3 0 53 43 90 887.3 0 90 44 151 887.3 0 151 45 83 887.3 0 83 46 641 887.3 0 641 47 369 887.3 0 369 48 635 887.3 0 635 49 87 887.3 0 87 50 70 887.3 0 70 51 56 887.3 0 56 52 70 887.3 0 70 53 70 887.3 0 70 54 102 887.3 0 102 55 142 887.3 0 142 56 201 887.3 0 201 57 89 887.3 0 89 58 65 887.3 0 65 59 57 887.3 0 57 60 52 887.3 0 52 61 79 887.3 0 79 62 76 887.3 0 76 63 58 887.3 0 58 64 56 887.3 0 56 65 53 887.3 0 53 66 58 887.3 0 58 67 101 887.3 0 101 68 201 887.3 0 201 69 102 887.3 0 102 70 111 887.3 0 111 71 117 887.3 0 117 72 66 887.3 0 66 73 90 887.3 0 90 74 75 887.3 0 75 75 66 887.3 0 66 76 48 887.3 0 48 77 41 887.3 0 41 78 44 887.3 0 44 79 66 887.3 0 66 80 63 887.3 0 63 81 60 887.3 0 60 82 83 887.3 0 83 83 78 887.3 0 78 84 82 887.3 0 82 85 63 887.3 0 63 86 48 887.3 0 48 87 75 887.3 0 75 88 107 887.3 0 107 89 73 887.3 0 73 90 123 887.3 0 123 91 101 887.3 0 101 92 94 887.3 0 94 93 82 887.3 0 82 94 122 887.3 0 122 95 128 887.3 0 128 96 138 887.3 0 138 97 84 887.3 0 84 98 88 887.3 0 88 99 86 887.3 0 86 100 73 887.3 0 73 101 90 887.3 0 90 102 99 887.3 0 99 103 144 887.3 0 144 104 591 887.3 0 591 105 928 887.3 0 928 106 2182 887.3 0 2182 107 616 887.3 0 616 108 347 887.3 0 347 109 151 887.3 0 151 110 125 887.3 0 125 111 125 887.3 0 125 112 219 887.3 0 219 113 210 887.3 0 210 114 191 887.3 0 191 115 84 887.3 0 84 116 141 887.3 0 141 117 107 887.3 0 107 118 124 887.3 0 124 119 272 887.3 0 272 120 68 887.3 0 68 121 96 887.3 0 96 122 91 887.3 0 91 123 104 887.3 0 104 124 189 887.3 0 189 125 179 887.3 0 179 126 190 887.3 0 190 127 309 887.3 0 309 128 307 887.3 0 307 129 523 887.3 0 523 130 130 887.3 0 130 131 230 887.3 0 230 132 153 887.3 0 153 133 180 887.3 0 180 134 943 887.3 0 943 135 262 887.3 0 262 136 226 887.3 0 226 137 147 887.3 0 147 138 145 887.3 0 145 139 338 887.3 0 338 140 372 887.3 0 372 141 703 887.3 0 703 142 657 887.3 0 657 143 1213 887.3 0 1213 144 2261 887.3 0 2261 145 3390 887.3 0 3390 146 44891 887.3 0 44891 147 43708 887.3 0 43708 148 72472 887.3 0 72472 149 839 887.3 0 839 150 340 887.3 0 340 151 960 887.3 0 960 152 931 887.3 0 931 153 1292 887.3 0 1292 154 705 887.3 0 705 155 897 887.3 0 897 156 1166 887.3 0 1166 157 1640 887.3 0 1640 158 2732 887.3 0 2732 159 4257 887.3 0 4257 160 7553 887.3 0 7553 161 88386 887.3 0 88386 162 112133 887.3 0 112133 163 94329 887.3 0 94329 164 626 887.3 0 626 165 163 887.3 0 163 166 153 887.3 0 153 167 196 887.3 0 196 168 142 887.3 0 142 169 96 887.3 0 96 170 84 887.3 0 84 171 82 887.3 0 82 172 79 887.3 0 79 173 100 887.3 0 100 174 294 887.3 0 294 175 295 887.3 0 295 176 330 887.3 0 330 177 124 887.3 0 124 178 104 887.3 0 104 179 158 887.3 0 158 180 220 887.3 0 220 181 1359 887.3 0 1359 182 1690 887.3 0 1690 183 1258 887.3 0 1258 184 648 887.3 0 648 185 106 887.3 0 106 186 160 887.3 0 160 187 170 887.3 0 170 188 123 887.3 0 123 189 119 887.3 0 119 190 124 887.3 0 124 191 78 887.3 0 78 192 94 887.3 0 94 193 111 887.3 0 111 194 104 887.3 0 104 195 73 887.3 0 73 196 99 887.3 0 99 197 89 887.3 0 89 198 89 887.3 0 89 199 105 887.3 0 105 200 127 887.3 0 127 201 108 887.3 0 108 202 115 887.3 0 115 203 88 887.3 0 88 204 99 887.3 0 99 205 213 887.3 0 213 206 110 887.3 0 110 207 134 887.3 0 134 208 101 887.3 0 101 209 109 887.3 0 109 210 94 887.3 0 94 211 103 887.3 0 103 212 91 887.3 0 91 213 265 887.3 0 265 214 171 887.3 0 171 215 188 887.3 0 188 216 153 887.3 0 153 217 104 887.3 0 104 218 123 887.3 0 123 219 141 887.3 0 141 220 198 887.3 0 198 221 145 887.3 0 145 222 118 887.3 0 118 223 93 887.3 0 93 224 108 887.3 0 108 225 109 887.3 0 109 226 108 887.3 0 108 227 108 887.3 0 108 228 118 887.3 0 118 229 109 887.3 0 109 230 126 887.3 0 126 231 167 887.3 0 167 232 206 887.3 0 206 233 156 887.3 0 156 234 115 887.3 0 115 235 122 887.3 0 122 236 261 887.3 0 261 237 244 887.3 0 244 238 245 887.3 0 245 239 466 887.3 0 466 240 1319 887.3 0 1319 241 1459 887.3 0 1459 242 1309 887.3 0 1309 243 426 887.3 0 426 244 334 887.3 0 334 245 182 887.3 0 182 246 102 887.3 0 102 247 132 887.3 0 132 248 132 887.3 0 132 249 188 887.3 0 188 250 120 887.3 0 120 251 145 887.3 0 145 252 120 887.3 0 120 253 233 887.3 0 233 254 226 887.3 0 226 255 191 887.3 0 191 256 177 887.3 0 177 257 120 887.3 0 120 258 145 887.3 0 145 259 165 887.3 0 165 260 142 887.3 0 142 261 155 887.3 0 155 262 147 887.3 0 147 263 118 887.3 0 118 264 95 887.3 0 95 265 115 887.3 0 115 266 178 887.3 0 178 267 444 887.3 0 444 268 983 887.3 0 983 269 1158 887.3 0 1158 270 649 887.3 0 649 271 503 887.3 0 503 272 513 887.3 0 513 273 253 887.3 0 253 274 321 887.3 0 321 275 496 887.3 0 496 276 581 887.3 0 581 277 1196 887.3 0 1196 278 1046 887.3 0 1046 279 1515 887.3 0 1515 280 979 887.3 0 979 281 520 887.3 0 520 282 422 887.3 0 422 283 309 887.3 0 309 284 169 887.3 0 169 285 133 887.3 0 133 286 140 887.3 0 140 287 140 887.3 0 140 288 277 887.3 0 277 289 238 887.3 0 238 290 240 887.3 0 240 291 133 887.3 0 133 292 200 887.3 0 200 293 243 887.3 0 243 294 296 887.3 0 296 295 316 887.3 0 316 296 265 887.3 0 265 297 174 887.3 0 174 298 186 887.3 0 186 299 112 887.3 0 112 300 114 887.3 0 114 301 158 887.3 0 158 Finished in 28.88 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 522,860 Reads with adapters: 2,382 (0.5%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 2,350 (0.4%) Total basepairs processed: 77,312,619 bp Total written (filtered): 625,975 bp (0.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2382 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 3 0 1 19 28 0.0 3 0 24 2 1 1 20 1983 0.0 4 1902 69 7 3 2 21 14 0.0 4 3 10 0 1 22 3 0.0 4 1 1 1 23 165 0.0 4 159 3 0 3 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 28 121 0.0 4 0 0 0 0 121 33 9 0.0 4 0 0 0 0 9 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 71 1 0.0 4 1 79 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 0 0 0 2 84 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 1 93 1 0.0 4 0 0 0 0 1 100 1 0.0 4 1 104 2 0.0 4 1 0 0 0 1 105 1 0.0 4 0 1 108 1 0.0 4 0 0 0 1 109 2 0.0 4 0 0 0 0 2 110 1 0.0 4 0 0 0 1 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 124 5 0.0 4 0 0 0 0 5 126 2 0.0 4 0 0 0 1 1 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 132 3 0.0 4 0 0 0 0 3 133 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 137 2 0.0 4 0 0 0 0 2 142 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 2,350 Reads with adapters: 117 (5.0%) Reads written (passing filters): 2,350 (100.0%) Total basepairs processed: 625,975 bp Total written (filtered): 624,060 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 117 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.7% C: 11.1% G: 77.8% T: 3.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 9 36.7 0 9 9 10 0.0 0 1 9 10 21 0.0 1 1 20 11 44 0.0 1 4 40 12 30 0.0 1 3 27 210 1 0.0 1 1 267 2 0.0 1 2