This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 151.59 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 514,107 (16.2%) Reads that were too short: 11,174 (0.4%) Reads written (passing filters): 502,933 (15.9%) Total basepairs processed: 953,096,634 bp Total written (filtered): 94,058,277 bp (9.9%) === Adapter 1 === Sequence: TATCTG; Type: regular 5'; Length: 6; Trimmed: 514107 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 110 773.1 0 110 7 87 773.1 0 87 8 874 773.1 0 874 9 614 773.1 0 614 10 66 773.1 0 66 11 66 773.1 0 66 12 81 773.1 0 81 13 79 773.1 0 79 14 87 773.1 0 87 15 96 773.1 0 96 16 55 773.1 0 55 17 88 773.1 0 88 18 90 773.1 0 90 19 77 773.1 0 77 20 60 773.1 0 60 21 70 773.1 0 70 22 78 773.1 0 78 23 65 773.1 0 65 24 97 773.1 0 97 25 244 773.1 0 244 26 256 773.1 0 256 27 220 773.1 0 220 28 68 773.1 0 68 29 56 773.1 0 56 30 85 773.1 0 85 31 82 773.1 0 82 32 95 773.1 0 95 33 66 773.1 0 66 34 60 773.1 0 60 35 372 773.1 0 372 36 91 773.1 0 91 37 108 773.1 0 108 38 110 773.1 0 110 39 95 773.1 0 95 40 56 773.1 0 56 41 51 773.1 0 51 42 77 773.1 0 77 43 68 773.1 0 68 44 51 773.1 0 51 45 60 773.1 0 60 46 56 773.1 0 56 47 59 773.1 0 59 48 50 773.1 0 50 49 33 773.1 0 33 50 55 773.1 0 55 51 54 773.1 0 54 52 71 773.1 0 71 53 74 773.1 0 74 54 68 773.1 0 68 55 121 773.1 0 121 56 75 773.1 0 75 57 97 773.1 0 97 58 72 773.1 0 72 59 73 773.1 0 73 60 104 773.1 0 104 61 313 773.1 0 313 62 51 773.1 0 51 63 87 773.1 0 87 64 75 773.1 0 75 65 78 773.1 0 78 66 105 773.1 0 105 67 73 773.1 0 73 68 78 773.1 0 78 69 137 773.1 0 137 70 959 773.1 0 959 71 840 773.1 0 840 72 1025 773.1 0 1025 73 92 773.1 0 92 74 93 773.1 0 93 75 88 773.1 0 88 76 124 773.1 0 124 77 126 773.1 0 126 78 124 773.1 0 124 79 138 773.1 0 138 80 114 773.1 0 114 81 95 773.1 0 95 82 232 773.1 0 232 83 212 773.1 0 212 84 231 773.1 0 231 85 143 773.1 0 143 86 660 773.1 0 660 87 82 773.1 0 82 88 78 773.1 0 78 89 86 773.1 0 86 90 84 773.1 0 84 91 84 773.1 0 84 92 73 773.1 0 73 93 184 773.1 0 184 94 104 773.1 0 104 95 347 773.1 0 347 96 682 773.1 0 682 97 212 773.1 0 212 98 362 773.1 0 362 99 207 773.1 0 207 100 449 773.1 0 449 101 1130 773.1 0 1130 102 292 773.1 0 292 103 303 773.1 0 303 104 143 773.1 0 143 105 228 773.1 0 228 106 479 773.1 0 479 107 635 773.1 0 635 108 1062 773.1 0 1062 109 1346 773.1 0 1346 110 2048 773.1 0 2048 111 4345 773.1 0 4345 112 11120 773.1 0 11120 113 173764 773.1 0 173764 114 112841 773.1 0 112841 115 141214 773.1 0 141214 116 783 773.1 0 783 117 119 773.1 0 119 118 108 773.1 0 108 119 99 773.1 0 99 120 93 773.1 0 93 121 143 773.1 0 143 122 294 773.1 0 294 123 93 773.1 0 93 124 134 773.1 0 134 125 181 773.1 0 181 126 119 773.1 0 119 127 651 773.1 0 651 128 248 773.1 0 248 129 206 773.1 0 206 130 158 773.1 0 158 131 128 773.1 0 128 132 204 773.1 0 204 133 266 773.1 0 266 134 331 773.1 0 331 135 4682 773.1 0 4682 136 9342 773.1 0 9342 137 10043 773.1 0 10043 138 364 773.1 0 364 139 378 773.1 0 378 140 416 773.1 0 416 141 362 773.1 0 362 142 107 773.1 0 107 143 100 773.1 0 100 144 111 773.1 0 111 145 624 773.1 0 624 146 210 773.1 0 210 147 85 773.1 0 85 148 88 773.1 0 88 149 79 773.1 0 79 150 83 773.1 0 83 151 67 773.1 0 67 152 64 773.1 0 64 153 92 773.1 0 92 154 159 773.1 0 159 155 98 773.1 0 98 156 78 773.1 0 78 157 90 773.1 0 90 158 250 773.1 0 250 159 244 773.1 0 244 160 343 773.1 0 343 161 128 773.1 0 128 162 70 773.1 0 70 163 69 773.1 0 69 164 83 773.1 0 83 165 69 773.1 0 69 166 76 773.1 0 76 167 81 773.1 0 81 168 93 773.1 0 93 169 88 773.1 0 88 170 74 773.1 0 74 171 69 773.1 0 69 172 55 773.1 0 55 173 61 773.1 0 61 174 58 773.1 0 58 175 80 773.1 0 80 176 73 773.1 0 73 177 89 773.1 0 89 178 80 773.1 0 80 179 75 773.1 0 75 180 136 773.1 0 136 181 122 773.1 0 122 182 88 773.1 0 88 183 58 773.1 0 58 184 82 773.1 0 82 185 86 773.1 0 86 186 99 773.1 0 99 187 89 773.1 0 89 188 100 773.1 0 100 189 74 773.1 0 74 190 82 773.1 0 82 191 99 773.1 0 99 192 201 773.1 0 201 193 71 773.1 0 71 194 49 773.1 0 49 195 54 773.1 0 54 196 90 773.1 0 90 197 61 773.1 0 61 198 67 773.1 0 67 199 94 773.1 0 94 200 78 773.1 0 78 201 115 773.1 0 115 202 109 773.1 0 109 203 157 773.1 0 157 204 228 773.1 0 228 205 202 773.1 0 202 206 334 773.1 0 334 207 85 773.1 0 85 208 96 773.1 0 96 209 53 773.1 0 53 210 70 773.1 0 70 211 92 773.1 0 92 212 79 773.1 0 79 213 57 773.1 0 57 214 121 773.1 0 121 215 109 773.1 0 109 216 144 773.1 0 144 217 84 773.1 0 84 218 60 773.1 0 60 219 149 773.1 0 149 220 150 773.1 0 150 221 150 773.1 0 150 222 82 773.1 0 82 223 107 773.1 0 107 224 501 773.1 0 501 225 547 773.1 0 547 226 341 773.1 0 341 227 73 773.1 0 73 228 59 773.1 0 59 229 84 773.1 0 84 230 53 773.1 0 53 231 70 773.1 0 70 232 61 773.1 0 61 233 91 773.1 0 91 234 156 773.1 0 156 235 186 773.1 0 186 236 131 773.1 0 131 237 78 773.1 0 78 238 44 773.1 0 44 239 50 773.1 0 50 240 56 773.1 0 56 241 62 773.1 0 62 242 59 773.1 0 59 243 74 773.1 0 74 244 73 773.1 0 73 245 87 773.1 0 87 246 61 773.1 0 61 247 69 773.1 0 69 248 56 773.1 0 56 249 62 773.1 0 62 250 64 773.1 0 64 251 64 773.1 0 64 252 64 773.1 0 64 253 71 773.1 0 71 254 65 773.1 0 65 255 96 773.1 0 96 256 74 773.1 0 74 257 122 773.1 0 122 258 78 773.1 0 78 259 75 773.1 0 75 260 79 773.1 0 79 261 105 773.1 0 105 262 58 773.1 0 58 263 63 773.1 0 63 264 58 773.1 0 58 265 58 773.1 0 58 266 56 773.1 0 56 267 61 773.1 0 61 268 69 773.1 0 69 269 72 773.1 0 72 270 75 773.1 0 75 271 110 773.1 0 110 272 127 773.1 0 127 273 201 773.1 0 201 274 182 773.1 0 182 275 116 773.1 0 116 276 81 773.1 0 81 277 57 773.1 0 57 278 61 773.1 0 61 279 76 773.1 0 76 280 196 773.1 0 196 281 182 773.1 0 182 282 406 773.1 0 406 283 69 773.1 0 69 284 99 773.1 0 99 285 89 773.1 0 89 286 82 773.1 0 82 287 91 773.1 0 91 288 89 773.1 0 89 289 104 773.1 0 104 290 64 773.1 0 64 291 59 773.1 0 59 292 97 773.1 0 97 293 108 773.1 0 108 294 263 773.1 0 263 295 204 773.1 0 204 296 234 773.1 0 234 297 84 773.1 0 84 298 56 773.1 0 56 299 55 773.1 0 55 300 62 773.1 0 62 301 41 773.1 0 41 Finished in 29.31 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 502,933 Reads with adapters: 614 (0.1%) Reads that were too short: 42 (0.0%) Reads written (passing filters): 572 (0.1%) Total basepairs processed: 94,058,277 bp Total written (filtered): 154,131 bp (0.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 614 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 2 0.0 3 0 2 20 554 0.0 4 530 19 4 1 21 4 0.0 4 2 2 27 1 0.0 4 0 0 1 50 1 0.0 4 0 0 1 51 3 0.0 4 0 1 0 0 2 54 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 0 0 0 2 84 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 1 92 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 96 3 0.0 4 0 0 0 0 3 97 2 0.0 4 0 0 0 0 2 122 12 0.0 4 0 0 0 1 11 123 6 0.0 4 0 0 0 0 6 125 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 142 2 0.0 4 0 0 0 0 2 145 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 159 1 0.0 4 1 171 1 0.0 4 0 0 0 0 1 182 2 0.0 4 0 0 0 0 2 183 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (52 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 572 Reads with adapters: 249 (43.5%) Reads written (passing filters): 572 (100.0%) Total basepairs processed: 154,131 bp Total written (filtered): 151,391 bp (98.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 249 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.2% C: 6.0% G: 90.0% T: 0.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 9 215 0.0 0 22 193 10 27 0.0 1 0 27 11 3 0.0 1 0 3 12 2 0.0 1 0 2 208 1 0.0 1 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.