This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 150.08 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 194,905 (6.2%) Reads that were too short: 12,969 (0.4%) Reads written (passing filters): 181,936 (5.7%) Total basepairs processed: 953,096,634 bp Total written (filtered): 30,154,625 bp (3.2%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 194905 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 156 773.1 0 156 7 1919 773.1 0 1919 8 1837 773.1 0 1837 9 114 773.1 0 114 10 55 773.1 0 55 11 66 773.1 0 66 12 68 773.1 0 68 13 58 773.1 0 58 14 78 773.1 0 78 15 85 773.1 0 85 16 53 773.1 0 53 17 74 773.1 0 74 18 65 773.1 0 65 19 45 773.1 0 45 20 68 773.1 0 68 21 76 773.1 0 76 22 66 773.1 0 66 23 71 773.1 0 71 24 58 773.1 0 58 25 81 773.1 0 81 26 92 773.1 0 92 27 189 773.1 0 189 28 260 773.1 0 260 29 276 773.1 0 276 30 57 773.1 0 57 31 64 773.1 0 64 32 199 773.1 0 199 33 561 773.1 0 561 34 72 773.1 0 72 35 95 773.1 0 95 36 76 773.1 0 76 37 73 773.1 0 73 38 58 773.1 0 58 39 85 773.1 0 85 40 51 773.1 0 51 41 63 773.1 0 63 42 74 773.1 0 74 43 308 773.1 0 308 44 60 773.1 0 60 45 67 773.1 0 67 46 79 773.1 0 79 47 82 773.1 0 82 48 68 773.1 0 68 49 102 773.1 0 102 50 69 773.1 0 69 51 74 773.1 0 74 52 89 773.1 0 89 53 81 773.1 0 81 54 64 773.1 0 64 55 69 773.1 0 69 56 59 773.1 0 59 57 86 773.1 0 86 58 360 773.1 0 360 59 308 773.1 0 308 60 787 773.1 0 787 61 64 773.1 0 64 62 60 773.1 0 60 63 51 773.1 0 51 64 49 773.1 0 49 65 56 773.1 0 56 66 65 773.1 0 65 67 57 773.1 0 57 68 76 773.1 0 76 69 91 773.1 0 91 70 197 773.1 0 197 71 169 773.1 0 169 72 1127 773.1 0 1127 73 693 773.1 0 693 74 705 773.1 0 705 75 87 773.1 0 87 76 88 773.1 0 88 77 91 773.1 0 91 78 69 773.1 0 69 79 63 773.1 0 63 80 75 773.1 0 75 81 64 773.1 0 64 82 70 773.1 0 70 83 88 773.1 0 88 84 60 773.1 0 60 85 50 773.1 0 50 86 58 773.1 0 58 87 74 773.1 0 74 88 88 773.1 0 88 89 57 773.1 0 57 90 136 773.1 0 136 91 127 773.1 0 127 92 152 773.1 0 152 93 101 773.1 0 101 94 62 773.1 0 62 95 76 773.1 0 76 96 70 773.1 0 70 97 49 773.1 0 49 98 73 773.1 0 73 99 65 773.1 0 65 100 84 773.1 0 84 101 92 773.1 0 92 102 52 773.1 0 52 103 57 773.1 0 57 104 75 773.1 0 75 105 63 773.1 0 63 106 56 773.1 0 56 107 77 773.1 0 77 108 84 773.1 0 84 109 109 773.1 0 109 110 107 773.1 0 107 111 97 773.1 0 97 112 262 773.1 0 262 113 333 773.1 0 333 114 415 773.1 0 415 115 66 773.1 0 66 116 88 773.1 0 88 117 160 773.1 0 160 118 82 773.1 0 82 119 196 773.1 0 196 120 90 773.1 0 90 121 98 773.1 0 98 122 74 773.1 0 74 123 98 773.1 0 98 124 76 773.1 0 76 125 109 773.1 0 109 126 140 773.1 0 140 127 184 773.1 0 184 128 274 773.1 0 274 129 573 773.1 0 573 130 952 773.1 0 952 131 226 773.1 0 226 132 204 773.1 0 204 133 348 773.1 0 348 134 278 773.1 0 278 135 253 773.1 0 253 136 441 773.1 0 441 137 355 773.1 0 355 138 494 773.1 0 494 139 438 773.1 0 438 140 872 773.1 0 872 141 1353 773.1 0 1353 142 2043 773.1 0 2043 143 51590 773.1 0 51590 144 36314 773.1 0 36314 145 60471 773.1 0 60471 146 336 773.1 0 336 147 83 773.1 0 83 148 227 773.1 0 227 149 183 773.1 0 183 150 182 773.1 0 182 151 46 773.1 0 46 152 68 773.1 0 68 153 60 773.1 0 60 154 43 773.1 0 43 155 104 773.1 0 104 156 195 773.1 0 195 157 156 773.1 0 156 158 55 773.1 0 55 159 55 773.1 0 55 160 59 773.1 0 59 161 68 773.1 0 68 162 102 773.1 0 102 163 377 773.1 0 377 164 161 773.1 0 161 165 499 773.1 0 499 166 66 773.1 0 66 167 58 773.1 0 58 168 67 773.1 0 67 169 48 773.1 0 48 170 64 773.1 0 64 171 57 773.1 0 57 172 50 773.1 0 50 173 59 773.1 0 59 174 76 773.1 0 76 175 43 773.1 0 43 176 89 773.1 0 89 177 58 773.1 0 58 178 61 773.1 0 61 179 55 773.1 0 55 180 65 773.1 0 65 181 64 773.1 0 64 182 49 773.1 0 49 183 76 773.1 0 76 184 70 773.1 0 70 185 60 773.1 0 60 186 61 773.1 0 61 187 57 773.1 0 57 188 62 773.1 0 62 189 65 773.1 0 65 190 82 773.1 0 82 191 90 773.1 0 90 192 54 773.1 0 54 193 58 773.1 0 58 194 41 773.1 0 41 195 77 773.1 0 77 196 87 773.1 0 87 197 67 773.1 0 67 198 61 773.1 0 61 199 54 773.1 0 54 200 59 773.1 0 59 201 61 773.1 0 61 202 65 773.1 0 65 203 101 773.1 0 101 204 98 773.1 0 98 205 78 773.1 0 78 206 77 773.1 0 77 207 74 773.1 0 74 208 139 773.1 0 139 209 141 773.1 0 141 210 130 773.1 0 130 211 171 773.1 0 171 212 119 773.1 0 119 213 245 773.1 0 245 214 312 773.1 0 312 215 881 773.1 0 881 216 499 773.1 0 499 217 571 773.1 0 571 218 64 773.1 0 64 219 49 773.1 0 49 220 63 773.1 0 63 221 88 773.1 0 88 222 92 773.1 0 92 223 83 773.1 0 83 224 80 773.1 0 80 225 86 773.1 0 86 226 92 773.1 0 92 227 113 773.1 0 113 228 84 773.1 0 84 229 74 773.1 0 74 230 62 773.1 0 62 231 139 773.1 0 139 232 211 773.1 0 211 233 235 773.1 0 235 234 120 773.1 0 120 235 91 773.1 0 91 236 123 773.1 0 123 237 93 773.1 0 93 238 53 773.1 0 53 239 74 773.1 0 74 240 56 773.1 0 56 241 86 773.1 0 86 242 73 773.1 0 73 243 80 773.1 0 80 244 105 773.1 0 105 245 122 773.1 0 122 246 95 773.1 0 95 247 61 773.1 0 61 248 59 773.1 0 59 249 62 773.1 0 62 250 53 773.1 0 53 251 86 773.1 0 86 252 67 773.1 0 67 253 88 773.1 0 88 254 87 773.1 0 87 255 86 773.1 0 86 256 56 773.1 0 56 257 69 773.1 0 69 258 92 773.1 0 92 259 671 773.1 0 671 260 251 773.1 0 251 261 120 773.1 0 120 262 67 773.1 0 67 263 181 773.1 0 181 264 189 773.1 0 189 265 212 773.1 0 212 266 89 773.1 0 89 267 53 773.1 0 53 268 56 773.1 0 56 269 77 773.1 0 77 270 89 773.1 0 89 271 103 773.1 0 103 272 83 773.1 0 83 273 84 773.1 0 84 274 65 773.1 0 65 275 88 773.1 0 88 276 79 773.1 0 79 277 76 773.1 0 76 278 97 773.1 0 97 279 74 773.1 0 74 280 174 773.1 0 174 281 141 773.1 0 141 282 209 773.1 0 209 283 109 773.1 0 109 284 121 773.1 0 121 285 120 773.1 0 120 286 128 773.1 0 128 287 80 773.1 0 80 288 72 773.1 0 72 289 81 773.1 0 81 290 91 773.1 0 91 291 105 773.1 0 105 292 100 773.1 0 100 293 99 773.1 0 99 294 112 773.1 0 112 295 175 773.1 0 175 296 227 773.1 0 227 297 304 773.1 0 304 298 148 773.1 0 148 299 84 773.1 0 84 300 64 773.1 0 64 301 68 773.1 0 68 Finished in 10.50 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 181,936 Reads with adapters: 1,918 (1.1%) Reads that were too short: 90 (0.0%) Reads written (passing filters): 1,828 (1.0%) Total basepairs processed: 30,154,625 bp Total written (filtered): 498,395 bp (1.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1918 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 0 1 1 19 16 0.0 3 0 13 0 2 1 20 1793 0.0 4 1691 76 22 4 21 6 0.0 4 2 3 0 1 23 5 0.0 4 0 0 0 3 2 33 1 0.0 4 1 48 1 0.0 4 0 0 0 0 1 50 1 0.0 4 1 72 2 0.0 4 0 0 0 0 2 73 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 89 4 0.0 4 0 0 0 0 4 93 1 0.0 4 0 0 0 0 1 97 5 0.0 4 0 0 0 0 5 98 9 0.0 4 0 0 0 0 9 99 4 0.0 4 0 0 0 0 4 101 2 0.0 4 0 0 0 1 1 103 1 0.0 4 0 0 0 0 1 113 2 0.0 4 0 0 0 0 2 118 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 129 2 0.0 4 0 0 0 0 2 146 32 0.0 4 0 0 0 0 32 147 6 0.0 4 0 0 0 0 6 148 1 0.0 4 0 0 0 0 1 149 3 0.0 4 0 0 0 0 3 153 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 160 2 0.0 4 0 0 0 0 2 188 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 1 219 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (66 us/read; 0.91 M reads/minute). === Summary === Total reads processed: 1,828 Reads with adapters: 392 (21.4%) Reads written (passing filters): 1,828 (100.0%) Total basepairs processed: 498,395 bp Total written (filtered): 493,085 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 392 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.8% C: 6.9% G: 88.0% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 28.6 0 5 10 7 0.0 1 0 7 11 375 0.0 1 9 366 14 1 0.0 1 0 1 270 1 0.0 1 1 272 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.