This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 150.75 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 194,216 (6.1%) Reads that were too short: 12,078 (0.4%) Reads written (passing filters): 182,138 (5.8%) Total basepairs processed: 953,096,634 bp Total written (filtered): 30,210,476 bp (3.2%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 194216 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 122 773.1 0 122 7 2010 773.1 0 2010 8 1873 773.1 0 1873 9 98 773.1 0 98 10 72 773.1 0 72 11 64 773.1 0 64 12 53 773.1 0 53 13 48 773.1 0 48 14 68 773.1 0 68 15 55 773.1 0 55 16 57 773.1 0 57 17 75 773.1 0 75 18 60 773.1 0 60 19 68 773.1 0 68 20 91 773.1 0 91 21 86 773.1 0 86 22 71 773.1 0 71 23 85 773.1 0 85 24 74 773.1 0 74 25 92 773.1 0 92 26 98 773.1 0 98 27 105 773.1 0 105 28 92 773.1 0 92 29 127 773.1 0 127 30 66 773.1 0 66 31 77 773.1 0 77 32 209 773.1 0 209 33 640 773.1 0 640 34 94 773.1 0 94 35 89 773.1 0 89 36 70 773.1 0 70 37 75 773.1 0 75 38 78 773.1 0 78 39 90 773.1 0 90 40 76 773.1 0 76 41 78 773.1 0 78 42 94 773.1 0 94 43 324 773.1 0 324 44 72 773.1 0 72 45 98 773.1 0 98 46 77 773.1 0 77 47 84 773.1 0 84 48 72 773.1 0 72 49 104 773.1 0 104 50 92 773.1 0 92 51 78 773.1 0 78 52 96 773.1 0 96 53 96 773.1 0 96 54 98 773.1 0 98 55 77 773.1 0 77 56 87 773.1 0 87 57 116 773.1 0 116 58 371 773.1 0 371 59 338 773.1 0 338 60 881 773.1 0 881 61 60 773.1 0 60 62 102 773.1 0 102 63 54 773.1 0 54 64 76 773.1 0 76 65 59 773.1 0 59 66 70 773.1 0 70 67 67 773.1 0 67 68 85 773.1 0 85 69 101 773.1 0 101 70 136 773.1 0 136 71 142 773.1 0 142 72 1053 773.1 0 1053 73 709 773.1 0 709 74 746 773.1 0 746 75 75 773.1 0 75 76 79 773.1 0 79 77 97 773.1 0 97 78 84 773.1 0 84 79 71 773.1 0 71 80 79 773.1 0 79 81 94 773.1 0 94 82 85 773.1 0 85 83 83 773.1 0 83 84 62 773.1 0 62 85 72 773.1 0 72 86 81 773.1 0 81 87 84 773.1 0 84 88 97 773.1 0 97 89 76 773.1 0 76 90 142 773.1 0 142 91 112 773.1 0 112 92 166 773.1 0 166 93 141 773.1 0 141 94 88 773.1 0 88 95 86 773.1 0 86 96 113 773.1 0 113 97 64 773.1 0 64 98 67 773.1 0 67 99 102 773.1 0 102 100 98 773.1 0 98 101 105 773.1 0 105 102 81 773.1 0 81 103 74 773.1 0 74 104 96 773.1 0 96 105 90 773.1 0 90 106 83 773.1 0 83 107 99 773.1 0 99 108 101 773.1 0 101 109 123 773.1 0 123 110 92 773.1 0 92 111 101 773.1 0 101 112 175 773.1 0 175 113 198 773.1 0 198 114 188 773.1 0 188 115 76 773.1 0 76 116 109 773.1 0 109 117 160 773.1 0 160 118 107 773.1 0 107 119 233 773.1 0 233 120 105 773.1 0 105 121 110 773.1 0 110 122 87 773.1 0 87 123 120 773.1 0 120 124 98 773.1 0 98 125 131 773.1 0 131 126 151 773.1 0 151 127 194 773.1 0 194 128 308 773.1 0 308 129 577 773.1 0 577 130 989 773.1 0 989 131 209 773.1 0 209 132 195 773.1 0 195 133 371 773.1 0 371 134 344 773.1 0 344 135 229 773.1 0 229 136 435 773.1 0 435 137 364 773.1 0 364 138 439 773.1 0 439 139 424 773.1 0 424 140 767 773.1 0 767 141 1220 773.1 0 1220 142 1628 773.1 0 1628 143 51463 773.1 0 51463 144 36471 773.1 0 36471 145 60469 773.1 0 60469 146 323 773.1 0 323 147 84 773.1 0 84 148 97 773.1 0 97 149 125 773.1 0 125 150 111 773.1 0 111 151 63 773.1 0 63 152 88 773.1 0 88 153 77 773.1 0 77 154 52 773.1 0 52 155 103 773.1 0 103 156 195 773.1 0 195 157 168 773.1 0 168 158 87 773.1 0 87 159 76 773.1 0 76 160 78 773.1 0 78 161 97 773.1 0 97 162 102 773.1 0 102 163 380 773.1 0 380 164 134 773.1 0 134 165 445 773.1 0 445 166 67 773.1 0 67 167 85 773.1 0 85 168 65 773.1 0 65 169 76 773.1 0 76 170 68 773.1 0 68 171 81 773.1 0 81 172 62 773.1 0 62 173 70 773.1 0 70 174 75 773.1 0 75 175 70 773.1 0 70 176 84 773.1 0 84 177 66 773.1 0 66 178 71 773.1 0 71 179 65 773.1 0 65 180 85 773.1 0 85 181 58 773.1 0 58 182 62 773.1 0 62 183 70 773.1 0 70 184 73 773.1 0 73 185 74 773.1 0 74 186 52 773.1 0 52 187 70 773.1 0 70 188 93 773.1 0 93 189 71 773.1 0 71 190 82 773.1 0 82 191 82 773.1 0 82 192 81 773.1 0 81 193 69 773.1 0 69 194 67 773.1 0 67 195 81 773.1 0 81 196 78 773.1 0 78 197 78 773.1 0 78 198 79 773.1 0 79 199 73 773.1 0 73 200 83 773.1 0 83 201 99 773.1 0 99 202 82 773.1 0 82 203 104 773.1 0 104 204 108 773.1 0 108 205 91 773.1 0 91 206 90 773.1 0 90 207 78 773.1 0 78 208 74 773.1 0 74 209 91 773.1 0 91 210 119 773.1 0 119 211 89 773.1 0 89 212 102 773.1 0 102 213 139 773.1 0 139 214 296 773.1 0 296 215 1067 773.1 0 1067 216 537 773.1 0 537 217 774 773.1 0 774 218 65 773.1 0 65 219 63 773.1 0 63 220 68 773.1 0 68 221 86 773.1 0 86 222 60 773.1 0 60 223 79 773.1 0 79 224 99 773.1 0 99 225 92 773.1 0 92 226 71 773.1 0 71 227 91 773.1 0 91 228 76 773.1 0 76 229 64 773.1 0 64 230 69 773.1 0 69 231 95 773.1 0 95 232 88 773.1 0 88 233 92 773.1 0 92 234 104 773.1 0 104 235 84 773.1 0 84 236 99 773.1 0 99 237 78 773.1 0 78 238 62 773.1 0 62 239 73 773.1 0 73 240 67 773.1 0 67 241 89 773.1 0 89 242 66 773.1 0 66 243 74 773.1 0 74 244 92 773.1 0 92 245 90 773.1 0 90 246 96 773.1 0 96 247 58 773.1 0 58 248 70 773.1 0 70 249 77 773.1 0 77 250 71 773.1 0 71 251 88 773.1 0 88 252 71 773.1 0 71 253 97 773.1 0 97 254 100 773.1 0 100 255 98 773.1 0 98 256 69 773.1 0 69 257 104 773.1 0 104 258 86 773.1 0 86 259 610 773.1 0 610 260 257 773.1 0 257 261 137 773.1 0 137 262 63 773.1 0 63 263 113 773.1 0 113 264 114 773.1 0 114 265 108 773.1 0 108 266 79 773.1 0 79 267 62 773.1 0 62 268 63 773.1 0 63 269 98 773.1 0 98 270 87 773.1 0 87 271 108 773.1 0 108 272 85 773.1 0 85 273 87 773.1 0 87 274 86 773.1 0 86 275 82 773.1 0 82 276 91 773.1 0 91 277 85 773.1 0 85 278 88 773.1 0 88 279 98 773.1 0 98 280 119 773.1 0 119 281 109 773.1 0 109 282 151 773.1 0 151 283 113 773.1 0 113 284 92 773.1 0 92 285 111 773.1 0 111 286 109 773.1 0 109 287 91 773.1 0 91 288 79 773.1 0 79 289 88 773.1 0 88 290 90 773.1 0 90 291 93 773.1 0 93 292 81 773.1 0 81 293 93 773.1 0 93 294 99 773.1 0 99 295 124 773.1 0 124 296 145 773.1 0 145 297 172 773.1 0 172 298 109 773.1 0 109 299 90 773.1 0 90 300 83 773.1 0 83 301 74 773.1 0 74 Finished in 9.56 s (52 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 182,138 Reads with adapters: 2,057 (1.1%) Reads that were too short: 99 (0.1%) Reads written (passing filters): 1,958 (1.1%) Total basepairs processed: 30,210,476 bp Total written (filtered): 529,130 bp (1.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2057 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 16 0.0 3 0 16 17 1853 0.0 3 1828 23 1 1 18 4 0.0 3 1 2 1 20 4 0.0 3 0 0 0 4 28 3 0.0 3 0 0 0 3 29 1 0.0 3 1 31 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 48 45 0.0 3 0 0 0 45 50 1 0.0 3 0 0 0 1 51 3 0.0 3 0 0 0 3 59 4 0.0 3 0 0 0 4 62 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 74 4 0.0 3 0 0 0 4 79 3 0.0 3 0 0 0 3 89 1 0.0 3 1 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 1 99 7 0.0 3 0 0 0 7 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 106 3 0.0 3 0 0 0 3 108 2 0.0 3 0 0 0 2 124 16 0.0 3 0 0 3 13 126 1 0.0 3 0 0 0 1 135 24 0.0 3 0 0 0 24 139 1 0.0 3 0 0 0 1 149 4 0.0 3 0 0 0 4 150 37 0.0 3 0 0 2 35 151 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 1,958 Reads with adapters: 1 (0.1%) Reads written (passing filters): 1,958 (100.0%) Total basepairs processed: 529,130 bp Total written (filtered): 529,127 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 30.6 0 1