This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.66 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 327,227 (12.7%) Reads that were too short: 22,445 (0.9%) Reads written (passing filters): 304,782 (11.8%) Total basepairs processed: 774,927,510 bp Total written (filtered): 58,578,196 bp (7.6%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 327227 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 263 628.5 0 263 7 6825 628.5 0 6825 8 6540 628.5 0 6540 9 59 628.5 0 59 10 70 628.5 0 70 11 49 628.5 0 49 12 383 628.5 0 383 13 266 628.5 0 266 14 885 628.5 0 885 15 39 628.5 0 39 16 42 628.5 0 42 17 43 628.5 0 43 18 50 628.5 0 50 19 44 628.5 0 44 20 59 628.5 0 59 21 63 628.5 0 63 22 71 628.5 0 71 23 52 628.5 0 52 24 98 628.5 0 98 25 308 628.5 0 308 26 308 628.5 0 308 27 150 628.5 0 150 28 58 628.5 0 58 29 70 628.5 0 70 30 69 628.5 0 69 31 83 628.5 0 83 32 86 628.5 0 86 33 65 628.5 0 65 34 54 628.5 0 54 35 91 628.5 0 91 36 81 628.5 0 81 37 79 628.5 0 79 38 62 628.5 0 62 39 73 628.5 0 73 40 74 628.5 0 74 41 82 628.5 0 82 42 102 628.5 0 102 43 159 628.5 0 159 44 72 628.5 0 72 45 38 628.5 0 38 46 67 628.5 0 67 47 84 628.5 0 84 48 64 628.5 0 64 49 113 628.5 0 113 50 50 628.5 0 50 51 55 628.5 0 55 52 56 628.5 0 56 53 83 628.5 0 83 54 108 628.5 0 108 55 131 628.5 0 131 56 148 628.5 0 148 57 76 628.5 0 76 58 96 628.5 0 96 59 70 628.5 0 70 60 95 628.5 0 95 61 86 628.5 0 86 62 124 628.5 0 124 63 106 628.5 0 106 64 105 628.5 0 105 65 593 628.5 0 593 66 633 628.5 0 633 67 689 628.5 0 689 68 129 628.5 0 129 69 81 628.5 0 81 70 95 628.5 0 95 71 71 628.5 0 71 72 104 628.5 0 104 73 102 628.5 0 102 74 83 628.5 0 83 75 78 628.5 0 78 76 65 628.5 0 65 77 102 628.5 0 102 78 172 628.5 0 172 79 137 628.5 0 137 80 241 628.5 0 241 81 206 628.5 0 206 82 130 628.5 0 130 83 115 628.5 0 115 84 138 628.5 0 138 85 124 628.5 0 124 86 157 628.5 0 157 87 227 628.5 0 227 88 352 628.5 0 352 89 540 628.5 0 540 90 906 628.5 0 906 91 1723 628.5 0 1723 92 473 628.5 0 473 93 527 628.5 0 527 94 1001 628.5 0 1001 95 406 628.5 0 406 96 359 628.5 0 359 97 853 628.5 0 853 98 865 628.5 0 865 99 1236 628.5 0 1236 100 888 628.5 0 888 101 1380 628.5 0 1380 102 2020 628.5 0 2020 103 2843 628.5 0 2843 104 36627 628.5 0 36627 105 55970 628.5 0 55970 106 113895 628.5 0 113895 107 494 628.5 0 494 108 61 628.5 0 61 109 70 628.5 0 70 110 134 628.5 0 134 111 97 628.5 0 97 112 138 628.5 0 138 113 100 628.5 0 100 114 168 628.5 0 168 115 140 628.5 0 140 116 102 628.5 0 102 117 76 628.5 0 76 118 82 628.5 0 82 119 186 628.5 0 186 120 115 628.5 0 115 121 200 628.5 0 200 122 80 628.5 0 80 123 79 628.5 0 79 124 200 628.5 0 200 125 259 628.5 0 259 126 340 628.5 0 340 127 105 628.5 0 105 128 80 628.5 0 80 129 73 628.5 0 73 130 87 628.5 0 87 131 47 628.5 0 47 132 132 628.5 0 132 133 55 628.5 0 55 134 92 628.5 0 92 135 67 628.5 0 67 136 80 628.5 0 80 137 81 628.5 0 81 138 177 628.5 0 177 139 162 628.5 0 162 140 288 628.5 0 288 141 108 628.5 0 108 142 166 628.5 0 166 143 58 628.5 0 58 144 128 628.5 0 128 145 91 628.5 0 91 146 125 628.5 0 125 147 195 628.5 0 195 148 160 628.5 0 160 149 125 628.5 0 125 150 108 628.5 0 108 151 223 628.5 0 223 152 380 628.5 0 380 153 508 628.5 0 508 154 320 628.5 0 320 155 326 628.5 0 326 156 341 628.5 0 341 157 793 628.5 0 793 158 1134 628.5 0 1134 159 15373 628.5 0 15373 160 8998 628.5 0 8998 161 16286 628.5 0 16286 162 153 628.5 0 153 163 77 628.5 0 77 164 70 628.5 0 70 165 79 628.5 0 79 166 79 628.5 0 79 167 265 628.5 0 265 168 233 628.5 0 233 169 359 628.5 0 359 170 82 628.5 0 82 171 116 628.5 0 116 172 274 628.5 0 274 173 352 628.5 0 352 174 347 628.5 0 347 175 77 628.5 0 77 176 86 628.5 0 86 177 74 628.5 0 74 178 71 628.5 0 71 179 77 628.5 0 77 180 119 628.5 0 119 181 111 628.5 0 111 182 121 628.5 0 121 183 142 628.5 0 142 184 462 628.5 0 462 185 196 628.5 0 196 186 316 628.5 0 316 187 311 628.5 0 311 188 127 628.5 0 127 189 320 628.5 0 320 190 97 628.5 0 97 191 62 628.5 0 62 192 78 628.5 0 78 193 106 628.5 0 106 194 104 628.5 0 104 195 116 628.5 0 116 196 123 628.5 0 123 197 73 628.5 0 73 198 115 628.5 0 115 199 186 628.5 0 186 200 492 628.5 0 492 201 423 628.5 0 423 202 594 628.5 0 594 203 96 628.5 0 96 204 84 628.5 0 84 205 160 628.5 0 160 206 195 628.5 0 195 207 218 628.5 0 218 208 196 628.5 0 196 209 163 628.5 0 163 210 87 628.5 0 87 211 70 628.5 0 70 212 90 628.5 0 90 213 110 628.5 0 110 214 114 628.5 0 114 215 112 628.5 0 112 216 121 628.5 0 121 217 107 628.5 0 107 218 188 628.5 0 188 219 327 628.5 0 327 220 364 628.5 0 364 221 274 628.5 0 274 222 172 628.5 0 172 223 78 628.5 0 78 224 83 628.5 0 83 225 93 628.5 0 93 226 147 628.5 0 147 227 196 628.5 0 196 228 305 628.5 0 305 229 217 628.5 0 217 230 224 628.5 0 224 231 113 628.5 0 113 232 131 628.5 0 131 233 115 628.5 0 115 234 111 628.5 0 111 235 89 628.5 0 89 236 86 628.5 0 86 237 103 628.5 0 103 238 201 628.5 0 201 239 262 628.5 0 262 240 1722 628.5 0 1722 241 1028 628.5 0 1028 242 1770 628.5 0 1770 243 523 628.5 0 523 244 97 628.5 0 97 245 76 628.5 0 76 246 92 628.5 0 92 247 125 628.5 0 125 248 142 628.5 0 142 249 116 628.5 0 116 250 98 628.5 0 98 251 122 628.5 0 122 252 108 628.5 0 108 253 135 628.5 0 135 254 390 628.5 0 390 255 370 628.5 0 370 256 473 628.5 0 473 257 313 628.5 0 313 258 83 628.5 0 83 259 104 628.5 0 104 260 93 628.5 0 93 261 120 628.5 0 120 262 111 628.5 0 111 263 113 628.5 0 113 264 84 628.5 0 84 265 88 628.5 0 88 266 128 628.5 0 128 267 185 628.5 0 185 268 182 628.5 0 182 269 200 628.5 0 200 270 165 628.5 0 165 271 109 628.5 0 109 272 117 628.5 0 117 273 177 628.5 0 177 274 196 628.5 0 196 275 224 628.5 0 224 276 244 628.5 0 244 277 155 628.5 0 155 278 357 628.5 0 357 279 496 628.5 0 496 280 234 628.5 0 234 281 380 628.5 0 380 282 584 628.5 0 584 283 473 628.5 0 473 284 192 628.5 0 192 285 85 628.5 0 85 286 78 628.5 0 78 287 90 628.5 0 90 288 182 628.5 0 182 289 122 628.5 0 122 290 133 628.5 0 133 291 109 628.5 0 109 292 129 628.5 0 129 293 158 628.5 0 158 294 219 628.5 0 219 295 179 628.5 0 179 296 153 628.5 0 153 297 87 628.5 0 87 298 114 628.5 0 114 299 199 628.5 0 199 300 370 628.5 0 370 301 348 628.5 0 348 Finished in 12.57 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 304,782 Reads with adapters: 27,890 (9.2%) Reads that were too short: 127 (0.0%) Reads written (passing filters): 27,763 (9.1%) Total basepairs processed: 58,578,196 bp Total written (filtered): 5,341,017 bp (9.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 27890 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 2 0.0 3 0 0 1 0 1 19 102 0.0 3 0 78 11 10 3 20 6546 0.0 4 5956 453 110 18 9 21 19 0.0 4 1 16 1 1 22 8 0.0 4 1 0 5 1 1 23 46 0.0 4 15 5 2 16 8 25 1 0.0 4 0 0 0 0 1 26 3 0.0 4 0 0 0 1 2 27 1 0.0 4 0 0 0 0 1 29 3 0.0 4 0 0 0 0 3 30 20179 0.0 4 0 0 0 1 20178 31 1 0.0 4 0 0 0 0 1 32 7 0.0 4 0 0 0 0 7 33 66 0.0 4 0 0 0 3 63 41 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 53 2 0.0 4 0 0 0 0 2 59 1 0.0 4 0 0 0 0 1 67 2 0.0 4 0 0 0 0 2 69 13 0.0 4 0 0 0 0 13 75 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 1 85 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 109 4 0.0 4 0 0 0 0 4 110 4 0.0 4 0 0 0 0 4 111 4 0.0 4 0 0 0 1 3 112 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 118 2 0.0 4 0 0 0 0 2 119 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 740 0.0 4 0 0 0 0 740 128 4 0.0 4 0 0 0 0 4 129 9 0.0 4 0 0 0 0 9 130 2 0.0 4 0 0 0 0 2 136 2 0.0 4 0 0 0 0 2 137 7 0.0 4 0 0 0 0 7 138 14 0.0 4 0 0 0 0 14 139 4 0.0 4 0 0 0 0 4 145 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 160 2 0.0 4 0 0 0 0 2 162 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 1 167 2 0.0 4 0 0 0 0 2 170 1 0.0 4 0 0 0 0 1 176 2 0.0 4 0 0 0 0 2 177 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 184 2 0.0 4 0 0 0 0 2 185 10 0.0 4 0 0 0 0 10 186 3 0.0 4 0 0 0 0 3 187 4 0.0 4 0 0 0 0 4 188 11 0.0 4 0 0 0 0 11 189 2 0.0 4 0 0 0 0 2 192 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 199 2 0.0 4 0 0 0 0 2 200 4 0.0 4 0 0 0 1 3 207 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 228 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 282 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.80 s (29 us/read; 2.08 M reads/minute). === Summary === Total reads processed: 27,763 Reads with adapters: 2,142 (7.7%) Reads written (passing filters): 27,763 (100.0%) Total basepairs processed: 5,341,017 bp Total written (filtered): 5,319,399 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2142 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 11.7% C: 7.0% G: 79.3% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 86 433.8 0 86 4 157 108.4 0 157 5 5 27.1 0 5 6 2 6.8 0 2 7 1 1.7 0 1 9 10 0.1 0 1 9 10 106 0.0 1 7 99 11 1764 0.0 1 65 1699 12 10 0.0 1 0 10 14 1 0.0 1 0 1