This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.14 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 155,907 (6.1%) Reads that were too short: 27,381 (1.1%) Reads written (passing filters): 128,526 (5.0%) Total basepairs processed: 774,927,510 bp Total written (filtered): 28,125,831 bp (3.6%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 155907 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 782 628.5 0 782 7 24972 628.5 0 24972 8 19324 628.5 0 19324 9 62 628.5 0 62 10 52 628.5 0 52 11 84 628.5 0 84 12 63 628.5 0 63 13 66 628.5 0 66 14 57 628.5 0 57 15 45 628.5 0 45 16 46 628.5 0 46 17 68 628.5 0 68 18 42 628.5 0 42 19 45 628.5 0 45 20 88 628.5 0 88 21 60 628.5 0 60 22 60 628.5 0 60 23 63 628.5 0 63 24 70 628.5 0 70 25 57 628.5 0 57 26 56 628.5 0 56 27 83 628.5 0 83 28 98 628.5 0 98 29 637 628.5 0 637 30 138 628.5 0 138 31 942 628.5 0 942 32 54 628.5 0 54 33 37 628.5 0 37 34 62 628.5 0 62 35 68 628.5 0 68 36 103 628.5 0 103 37 104 628.5 0 104 38 59 628.5 0 59 39 97 628.5 0 97 40 71 628.5 0 71 41 39 628.5 0 39 42 70 628.5 0 70 43 67 628.5 0 67 44 82 628.5 0 82 45 71 628.5 0 71 46 84 628.5 0 84 47 61 628.5 0 61 48 80 628.5 0 80 49 78 628.5 0 78 50 72 628.5 0 72 51 79 628.5 0 79 52 64 628.5 0 64 53 79 628.5 0 79 54 85 628.5 0 85 55 72 628.5 0 72 56 74 628.5 0 74 57 81 628.5 0 81 58 66 628.5 0 66 59 86 628.5 0 86 60 76 628.5 0 76 61 75 628.5 0 75 62 90 628.5 0 90 63 71 628.5 0 71 64 76 628.5 0 76 65 91 628.5 0 91 66 85 628.5 0 85 67 97 628.5 0 97 68 107 628.5 0 107 69 115 628.5 0 115 70 994 628.5 0 994 71 387 628.5 0 387 72 539 628.5 0 539 73 81 628.5 0 81 74 85 628.5 0 85 75 56 628.5 0 56 76 67 628.5 0 67 77 75 628.5 0 75 78 89 628.5 0 89 79 115 628.5 0 115 80 83 628.5 0 83 81 127 628.5 0 127 82 202 628.5 0 202 83 176 628.5 0 176 84 78 628.5 0 78 85 71 628.5 0 71 86 78 628.5 0 78 87 67 628.5 0 67 88 52 628.5 0 52 89 48 628.5 0 48 90 56 628.5 0 56 91 68 628.5 0 68 92 57 628.5 0 57 93 68 628.5 0 68 94 77 628.5 0 77 95 75 628.5 0 75 96 58 628.5 0 58 97 127 628.5 0 127 98 117 628.5 0 117 99 94 628.5 0 94 100 92 628.5 0 92 101 122 628.5 0 122 102 98 628.5 0 98 103 81 628.5 0 81 104 74 628.5 0 74 105 103 628.5 0 103 106 74 628.5 0 74 107 107 628.5 0 107 108 112 628.5 0 112 109 178 628.5 0 178 110 252 628.5 0 252 111 487 628.5 0 487 112 211 628.5 0 211 113 199 628.5 0 199 114 262 628.5 0 262 115 159 628.5 0 159 116 174 628.5 0 174 117 278 628.5 0 278 118 270 628.5 0 270 119 355 628.5 0 355 120 324 628.5 0 324 121 449 628.5 0 449 122 548 628.5 0 548 123 924 628.5 0 924 124 13487 628.5 0 13487 125 18205 628.5 0 18205 126 17297 628.5 0 17297 127 582 628.5 0 582 128 132 628.5 0 132 129 188 628.5 0 188 130 100 628.5 0 100 131 105 628.5 0 105 132 84 628.5 0 84 133 76 628.5 0 76 134 93 628.5 0 93 135 126 628.5 0 126 136 223 628.5 0 223 137 174 628.5 0 174 138 283 628.5 0 283 139 232 628.5 0 232 140 563 628.5 0 563 141 213 628.5 0 213 142 213 628.5 0 213 143 94 628.5 0 94 144 125 628.5 0 125 145 242 628.5 0 242 146 146 628.5 0 146 147 143 628.5 0 143 148 222 628.5 0 222 149 2760 628.5 0 2760 150 2196 628.5 0 2196 151 3443 628.5 0 3443 152 114 628.5 0 114 153 69 628.5 0 69 154 84 628.5 0 84 155 87 628.5 0 87 156 92 628.5 0 92 157 101 628.5 0 101 158 101 628.5 0 101 159 84 628.5 0 84 160 78 628.5 0 78 161 80 628.5 0 80 162 142 628.5 0 142 163 228 628.5 0 228 164 244 628.5 0 244 165 167 628.5 0 167 166 73 628.5 0 73 167 71 628.5 0 71 168 64 628.5 0 64 169 89 628.5 0 89 170 71 628.5 0 71 171 86 628.5 0 86 172 68 628.5 0 68 173 64 628.5 0 64 174 73 628.5 0 73 175 70 628.5 0 70 176 63 628.5 0 63 177 70 628.5 0 70 178 64 628.5 0 64 179 82 628.5 0 82 180 91 628.5 0 91 181 224 628.5 0 224 182 192 628.5 0 192 183 289 628.5 0 289 184 103 628.5 0 103 185 122 628.5 0 122 186 102 628.5 0 102 187 69 628.5 0 69 188 131 628.5 0 131 189 299 628.5 0 299 190 500 628.5 0 500 191 55 628.5 0 55 192 65 628.5 0 65 193 50 628.5 0 50 194 75 628.5 0 75 195 83 628.5 0 83 196 78 628.5 0 78 197 79 628.5 0 79 198 114 628.5 0 114 199 76 628.5 0 76 200 56 628.5 0 56 201 59 628.5 0 59 202 63 628.5 0 63 203 125 628.5 0 125 204 143 628.5 0 143 205 193 628.5 0 193 206 236 628.5 0 236 207 120 628.5 0 120 208 209 628.5 0 209 209 250 628.5 0 250 210 105 628.5 0 105 211 88 628.5 0 88 212 105 628.5 0 105 213 116 628.5 0 116 214 104 628.5 0 104 215 85 628.5 0 85 216 96 628.5 0 96 217 85 628.5 0 85 218 90 628.5 0 90 219 114 628.5 0 114 220 177 628.5 0 177 221 225 628.5 0 225 222 249 628.5 0 249 223 1048 628.5 0 1048 224 1017 628.5 0 1017 225 4932 628.5 0 4932 226 3061 628.5 0 3061 227 7143 628.5 0 7143 228 97 628.5 0 97 229 86 628.5 0 86 230 91 628.5 0 91 231 81 628.5 0 81 232 94 628.5 0 94 233 97 628.5 0 97 234 117 628.5 0 117 235 116 628.5 0 116 236 137 628.5 0 137 237 143 628.5 0 143 238 99 628.5 0 99 239 84 628.5 0 84 240 106 628.5 0 106 241 97 628.5 0 97 242 107 628.5 0 107 243 173 628.5 0 173 244 217 628.5 0 217 245 230 628.5 0 230 246 198 628.5 0 198 247 68 628.5 0 68 248 51 628.5 0 51 249 64 628.5 0 64 250 65 628.5 0 65 251 82 628.5 0 82 252 78 628.5 0 78 253 94 628.5 0 94 254 109 628.5 0 109 255 106 628.5 0 106 256 157 628.5 0 157 257 147 628.5 0 147 258 143 628.5 0 143 259 111 628.5 0 111 260 91 628.5 0 91 261 77 628.5 0 77 262 101 628.5 0 101 263 143 628.5 0 143 264 136 628.5 0 136 265 219 628.5 0 219 266 156 628.5 0 156 267 143 628.5 0 143 268 168 628.5 0 168 269 146 628.5 0 146 270 70 628.5 0 70 271 51 628.5 0 51 272 82 628.5 0 82 273 79 628.5 0 79 274 95 628.5 0 95 275 92 628.5 0 92 276 78 628.5 0 78 277 80 628.5 0 80 278 66 628.5 0 66 279 79 628.5 0 79 280 80 628.5 0 80 281 80 628.5 0 80 282 59 628.5 0 59 283 66 628.5 0 66 284 53 628.5 0 53 285 48 628.5 0 48 286 69 628.5 0 69 287 59 628.5 0 59 288 60 628.5 0 60 289 67 628.5 0 67 290 51 628.5 0 51 291 54 628.5 0 54 292 45 628.5 0 45 293 69 628.5 0 69 294 57 628.5 0 57 295 86 628.5 0 86 296 48 628.5 0 48 297 63 628.5 0 63 298 69 628.5 0 69 299 92 628.5 0 92 300 70 628.5 0 70 301 60 628.5 0 60 Finished in 5.89 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 128,526 Reads with adapters: 20,451 (15.9%) Reads that were too short: 72 (0.1%) Reads written (passing filters): 20,379 (15.9%) Total basepairs processed: 28,125,831 bp Total written (filtered): 5,496,062 bp (19.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 20451 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 2 1 2 19 35 0.0 3 0 13 11 4 7 20 2005 0.0 4 1568 343 52 33 9 21 17693 0.0 4 16114 1225 253 67 34 22 40 0.0 4 8 30 2 23 13 0.0 4 0 0 4 8 1 24 100 0.0 4 2 0 0 65 33 25 8 0.0 4 0 0 0 0 8 27 16 0.0 4 0 0 0 0 16 31 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 1 45 1 0.0 4 0 0 0 0 1 65 3 0.0 4 1 0 0 0 2 66 2 0.0 4 1 1 68 1 0.0 4 0 1 76 1 0.0 4 0 0 0 0 1 79 2 0.0 4 0 0 0 1 1 83 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 1 1 92 2 0.0 4 0 0 0 0 2 96 5 0.0 4 0 0 0 0 5 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 112 6 0.0 4 0 0 0 0 6 114 1 0.0 4 0 0 0 1 116 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 1 121 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 83 0.0 4 0 0 0 0 83 128 361 0.0 4 0 0 0 0 361 129 2 0.0 4 0 0 0 0 2 131 5 0.0 4 0 0 0 0 5 140 2 0.0 4 0 0 0 0 2 141 2 0.0 4 0 0 0 0 2 142 2 0.0 4 0 0 0 0 2 143 1 0.0 4 0 0 0 0 1 154 2 0.0 4 0 0 0 0 2 156 1 0.0 4 0 0 0 0 1 164 2 0.0 4 0 0 0 0 2 165 2 0.0 4 0 0 0 0 2 171 1 0.0 4 0 0 0 0 1 172 3 0.0 4 0 0 0 0 3 173 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 175 2 0.0 4 0 0 1 0 1 187 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 4 0.0 4 0 0 0 0 4 230 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 269 1 0.0 4 0 0 0 0 1 284 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.74 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 20,379 Reads with adapters: 4,745 (23.3%) Reads written (passing filters): 20,379 (100.0%) Total basepairs processed: 5,496,062 bp Total written (filtered): 5,437,005 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4745 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.0% C: 10.3% G: 83.8% T: 2.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 11 318.4 0 11 4 3 79.6 0 3 5 1 19.9 0 1 7 2 1.2 0 2 8 1 0.3 0 1 9 546 0.1 0 81 465 10 3263 0.0 1 300 2963 11 833 0.0 1 36 797 12 16 0.0 1 0 16 13 15 0.0 1 0 15 73 1 0.0 1 1 168 1 0.0 1 0 1 169 1 0.0 1 1 220 1 0.0 1 1 223 44 0.0 1 36 8 224 3 0.0 1 2 1 259 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.