This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.64 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 156,639 (6.1%) Reads that were too short: 28,045 (1.1%) Reads written (passing filters): 128,594 (5.0%) Total basepairs processed: 774,927,510 bp Total written (filtered): 28,361,690 bp (3.7%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 156639 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 583 628.5 0 583 7 24555 628.5 0 24555 8 22679 628.5 0 22679 9 41 628.5 0 41 10 24 628.5 0 24 11 66 628.5 0 66 12 43 628.5 0 43 13 39 628.5 0 39 14 32 628.5 0 32 15 29 628.5 0 29 16 32 628.5 0 32 17 54 628.5 0 54 18 33 628.5 0 33 19 34 628.5 0 34 20 52 628.5 0 52 21 49 628.5 0 49 22 41 628.5 0 41 23 41 628.5 0 41 24 44 628.5 0 44 25 31 628.5 0 31 26 54 628.5 0 54 27 76 628.5 0 76 28 80 628.5 0 80 29 619 628.5 0 619 30 89 628.5 0 89 31 873 628.5 0 873 32 33 628.5 0 33 33 32 628.5 0 32 34 36 628.5 0 36 35 49 628.5 0 49 36 62 628.5 0 62 37 55 628.5 0 55 38 57 628.5 0 57 39 60 628.5 0 60 40 52 628.5 0 52 41 30 628.5 0 30 42 38 628.5 0 38 43 37 628.5 0 37 44 50 628.5 0 50 45 44 628.5 0 44 46 52 628.5 0 52 47 55 628.5 0 55 48 59 628.5 0 59 49 51 628.5 0 51 50 40 628.5 0 40 51 34 628.5 0 34 52 38 628.5 0 38 53 42 628.5 0 42 54 49 628.5 0 49 55 33 628.5 0 33 56 43 628.5 0 43 57 48 628.5 0 48 58 42 628.5 0 42 59 62 628.5 0 62 60 55 628.5 0 55 61 53 628.5 0 53 62 47 628.5 0 47 63 40 628.5 0 40 64 60 628.5 0 60 65 62 628.5 0 62 66 55 628.5 0 55 67 76 628.5 0 76 68 76 628.5 0 76 69 99 628.5 0 99 70 1072 628.5 0 1072 71 404 628.5 0 404 72 540 628.5 0 540 73 43 628.5 0 43 74 39 628.5 0 39 75 38 628.5 0 38 76 34 628.5 0 34 77 41 628.5 0 41 78 43 628.5 0 43 79 61 628.5 0 61 80 55 628.5 0 55 81 97 628.5 0 97 82 140 628.5 0 140 83 96 628.5 0 96 84 51 628.5 0 51 85 56 628.5 0 56 86 55 628.5 0 55 87 39 628.5 0 39 88 39 628.5 0 39 89 34 628.5 0 34 90 46 628.5 0 46 91 43 628.5 0 43 92 48 628.5 0 48 93 53 628.5 0 53 94 42 628.5 0 42 95 48 628.5 0 48 96 41 628.5 0 41 97 79 628.5 0 79 98 78 628.5 0 78 99 80 628.5 0 80 100 77 628.5 0 77 101 69 628.5 0 69 102 61 628.5 0 61 103 57 628.5 0 57 104 53 628.5 0 53 105 61 628.5 0 61 106 58 628.5 0 58 107 100 628.5 0 100 108 91 628.5 0 91 109 149 628.5 0 149 110 228 628.5 0 228 111 487 628.5 0 487 112 159 628.5 0 159 113 162 628.5 0 162 114 282 628.5 0 282 115 151 628.5 0 151 116 135 628.5 0 135 117 262 628.5 0 262 118 233 628.5 0 233 119 296 628.5 0 296 120 241 628.5 0 241 121 344 628.5 0 344 122 433 628.5 0 433 123 660 628.5 0 660 124 13845 628.5 0 13845 125 19198 628.5 0 19198 126 18551 628.5 0 18551 127 546 628.5 0 546 128 81 628.5 0 81 129 85 628.5 0 85 130 50 628.5 0 50 131 48 628.5 0 48 132 35 628.5 0 35 133 52 628.5 0 52 134 50 628.5 0 50 135 103 628.5 0 103 136 131 628.5 0 131 137 81 628.5 0 81 138 142 628.5 0 142 139 186 628.5 0 186 140 522 628.5 0 522 141 180 628.5 0 180 142 153 628.5 0 153 143 69 628.5 0 69 144 86 628.5 0 86 145 181 628.5 0 181 146 113 628.5 0 113 147 93 628.5 0 93 148 154 628.5 0 154 149 3055 628.5 0 3055 150 2314 628.5 0 2314 151 3893 628.5 0 3893 152 94 628.5 0 94 153 42 628.5 0 42 154 42 628.5 0 42 155 45 628.5 0 45 156 49 628.5 0 49 157 56 628.5 0 56 158 60 628.5 0 60 159 48 628.5 0 48 160 39 628.5 0 39 161 40 628.5 0 40 162 59 628.5 0 59 163 215 628.5 0 215 164 214 628.5 0 214 165 169 628.5 0 169 166 41 628.5 0 41 167 34 628.5 0 34 168 43 628.5 0 43 169 45 628.5 0 45 170 30 628.5 0 30 171 64 628.5 0 64 172 38 628.5 0 38 173 41 628.5 0 41 174 43 628.5 0 43 175 45 628.5 0 45 176 48 628.5 0 48 177 49 628.5 0 49 178 44 628.5 0 44 179 53 628.5 0 53 180 55 628.5 0 55 181 143 628.5 0 143 182 110 628.5 0 110 183 190 628.5 0 190 184 48 628.5 0 48 185 64 628.5 0 64 186 48 628.5 0 48 187 51 628.5 0 51 188 118 628.5 0 118 189 319 628.5 0 319 190 573 628.5 0 573 191 38 628.5 0 38 192 26 628.5 0 26 193 35 628.5 0 35 194 61 628.5 0 61 195 40 628.5 0 40 196 62 628.5 0 62 197 72 628.5 0 72 198 75 628.5 0 75 199 44 628.5 0 44 200 27 628.5 0 27 201 25 628.5 0 25 202 34 628.5 0 34 203 40 628.5 0 40 204 64 628.5 0 64 205 60 628.5 0 60 206 75 628.5 0 75 207 84 628.5 0 84 208 110 628.5 0 110 209 101 628.5 0 101 210 87 628.5 0 87 211 72 628.5 0 72 212 53 628.5 0 53 213 103 628.5 0 103 214 64 628.5 0 64 215 63 628.5 0 63 216 63 628.5 0 63 217 52 628.5 0 52 218 63 628.5 0 63 219 83 628.5 0 83 220 133 628.5 0 133 221 202 628.5 0 202 222 241 628.5 0 241 223 1112 628.5 0 1112 224 922 628.5 0 922 225 6118 628.5 0 6118 226 3988 628.5 0 3988 227 9957 628.5 0 9957 228 92 628.5 0 92 229 61 628.5 0 61 230 56 628.5 0 56 231 43 628.5 0 43 232 54 628.5 0 54 233 43 628.5 0 43 234 59 628.5 0 59 235 67 628.5 0 67 236 85 628.5 0 85 237 63 628.5 0 63 238 52 628.5 0 52 239 54 628.5 0 54 240 58 628.5 0 58 241 63 628.5 0 63 242 64 628.5 0 64 243 80 628.5 0 80 244 92 628.5 0 92 245 104 628.5 0 104 246 58 628.5 0 58 247 42 628.5 0 42 248 41 628.5 0 41 249 42 628.5 0 42 250 44 628.5 0 44 251 44 628.5 0 44 252 43 628.5 0 43 253 68 628.5 0 68 254 58 628.5 0 58 255 64 628.5 0 64 256 84 628.5 0 84 257 71 628.5 0 71 258 68 628.5 0 68 259 54 628.5 0 54 260 58 628.5 0 58 261 48 628.5 0 48 262 40 628.5 0 40 263 64 628.5 0 64 264 66 628.5 0 66 265 97 628.5 0 97 266 93 628.5 0 93 267 73 628.5 0 73 268 90 628.5 0 90 269 59 628.5 0 59 270 44 628.5 0 44 271 47 628.5 0 47 272 33 628.5 0 33 273 41 628.5 0 41 274 40 628.5 0 40 275 51 628.5 0 51 276 51 628.5 0 51 277 49 628.5 0 49 278 42 628.5 0 42 279 45 628.5 0 45 280 59 628.5 0 59 281 77 628.5 0 77 282 56 628.5 0 56 283 57 628.5 0 57 284 44 628.5 0 44 285 53 628.5 0 53 286 45 628.5 0 45 287 40 628.5 0 40 288 33 628.5 0 33 289 33 628.5 0 33 290 31 628.5 0 31 291 31 628.5 0 31 292 35 628.5 0 35 293 41 628.5 0 41 294 38 628.5 0 38 295 64 628.5 0 64 296 48 628.5 0 48 297 38 628.5 0 38 298 42 628.5 0 42 299 40 628.5 0 40 300 58 628.5 0 58 301 36 628.5 0 36 Finished in 5.07 s (39 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 128,594 Reads with adapters: 25,613 (19.9%) Reads that were too short: 39 (0.0%) Reads written (passing filters): 25,574 (19.9%) Total basepairs processed: 28,361,690 bp Total written (filtered): 6,867,230 bp (24.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 25613 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 20 0.0 3 0 15 5 17 1800 0.0 3 1616 182 2 18 22525 0.0 3 22121 395 9 19 42 0.0 3 3 34 4 1 20 8 0.0 3 0 0 4 4 21 6 0.0 3 0 0 0 6 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 7 0.0 3 0 0 0 7 28 1 0.0 3 0 0 0 1 29 3 0.0 3 0 0 0 3 33 1 0.0 3 0 0 0 1 42 145 0.0 3 0 0 0 145 44 1 0.0 3 0 0 0 1 45 21 0.0 3 0 0 1 20 53 1 0.0 3 1 58 1 0.0 3 0 0 0 1 62 1 0.0 3 1 64 3 0.0 3 3 67 1 0.0 3 0 0 0 1 68 974 0.0 3 0 0 1 973 69 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 93 3 0.0 3 0 0 0 3 95 1 0.0 3 0 0 0 1 102 11 0.0 3 0 0 0 11 107 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 144 8 0.0 3 0 0 0 8 154 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 163 1 0.0 3 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 174 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.88 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 25,574 Reads with adapters: 246 (1.0%) Reads written (passing filters): 25,574 (100.0%) Total basepairs processed: 6,867,230 bp Total written (filtered): 6,866,418 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 246 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 59.8% C: 7.7% G: 31.7% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 172 399.6 0 172 4 74 99.9 0 74