This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.16 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 311,558 (10.9%) Reads that were too short: 24,267 (0.9%) Reads written (passing filters): 287,291 (10.1%) Total basepairs processed: 858,556,748 bp Total written (filtered): 45,404,455 bp (5.3%) === Adapter 1 === Sequence: CACTCT; Type: regular 5'; Length: 6; Trimmed: 311558 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 798 696.4 0 798 7 7404 696.4 0 7404 8 169 696.4 0 169 9 7838 696.4 0 7838 10 77 696.4 0 77 11 31 696.4 0 31 12 124 696.4 0 124 13 33 696.4 0 33 14 80 696.4 0 80 15 29 696.4 0 29 16 23 696.4 0 23 17 23 696.4 0 23 18 35 696.4 0 35 19 24 696.4 0 24 20 39 696.4 0 39 21 42 696.4 0 42 22 35 696.4 0 35 23 30 696.4 0 30 24 36 696.4 0 36 25 34 696.4 0 34 26 31 696.4 0 31 27 35 696.4 0 35 28 27 696.4 0 27 29 30 696.4 0 30 30 58 696.4 0 58 31 88 696.4 0 88 32 63 696.4 0 63 33 55 696.4 0 55 34 71 696.4 0 71 35 39 696.4 0 39 36 64 696.4 0 64 37 47 696.4 0 47 38 38 696.4 0 38 39 40 696.4 0 40 40 40 696.4 0 40 41 37 696.4 0 37 42 55 696.4 0 55 43 60 696.4 0 60 44 101 696.4 0 101 45 59 696.4 0 59 46 603 696.4 0 603 47 188 696.4 0 188 48 234 696.4 0 234 49 49 696.4 0 49 50 46 696.4 0 46 51 33 696.4 0 33 52 36 696.4 0 36 53 36 696.4 0 36 54 140 696.4 0 140 55 99 696.4 0 99 56 183 696.4 0 183 57 47 696.4 0 47 58 47 696.4 0 47 59 51 696.4 0 51 60 47 696.4 0 47 61 36 696.4 0 36 62 41 696.4 0 41 63 25 696.4 0 25 64 24 696.4 0 24 65 40 696.4 0 40 66 32 696.4 0 32 67 61 696.4 0 61 68 52 696.4 0 52 69 76 696.4 0 76 70 99 696.4 0 99 71 59 696.4 0 59 72 122 696.4 0 122 73 58 696.4 0 58 74 40 696.4 0 40 75 37 696.4 0 37 76 38 696.4 0 38 77 44 696.4 0 44 78 38 696.4 0 38 79 54 696.4 0 54 80 37 696.4 0 37 81 41 696.4 0 41 82 56 696.4 0 56 83 53 696.4 0 53 84 60 696.4 0 60 85 56 696.4 0 56 86 37 696.4 0 37 87 76 696.4 0 76 88 84 696.4 0 84 89 71 696.4 0 71 90 114 696.4 0 114 91 93 696.4 0 93 92 120 696.4 0 120 93 71 696.4 0 71 94 121 696.4 0 121 95 149 696.4 0 149 96 104 696.4 0 104 97 75 696.4 0 75 98 50 696.4 0 50 99 54 696.4 0 54 100 46 696.4 0 46 101 59 696.4 0 59 102 51 696.4 0 51 103 92 696.4 0 92 104 743 696.4 0 743 105 866 696.4 0 866 106 2525 696.4 0 2525 107 213 696.4 0 213 108 199 696.4 0 199 109 117 696.4 0 117 110 109 696.4 0 109 111 75 696.4 0 75 112 244 696.4 0 244 113 112 696.4 0 112 114 152 696.4 0 152 115 52 696.4 0 52 116 83 696.4 0 83 117 58 696.4 0 58 118 80 696.4 0 80 119 240 696.4 0 240 120 56 696.4 0 56 121 82 696.4 0 82 122 76 696.4 0 76 123 74 696.4 0 74 124 106 696.4 0 106 125 88 696.4 0 88 126 162 696.4 0 162 127 307 696.4 0 307 128 246 696.4 0 246 129 388 696.4 0 388 130 94 696.4 0 94 131 213 696.4 0 213 132 129 696.4 0 129 133 147 696.4 0 147 134 453 696.4 0 453 135 183 696.4 0 183 136 175 696.4 0 175 137 104 696.4 0 104 138 198 696.4 0 198 139 261 696.4 0 261 140 343 696.4 0 343 141 540 696.4 0 540 142 634 696.4 0 634 143 1051 696.4 0 1051 144 1919 696.4 0 1919 145 3500 696.4 0 3500 146 43364 696.4 0 43364 147 50007 696.4 0 50007 148 26188 696.4 0 26188 149 499 696.4 0 499 150 275 696.4 0 275 151 624 696.4 0 624 152 537 696.4 0 537 153 628 696.4 0 628 154 453 696.4 0 453 155 444 696.4 0 444 156 595 696.4 0 595 157 663 696.4 0 663 158 1175 696.4 0 1175 159 2303 696.4 0 2303 160 3898 696.4 0 3898 161 50301 696.4 0 50301 162 35181 696.4 0 35181 163 22520 696.4 0 22520 164 228 696.4 0 228 165 187 696.4 0 187 166 152 696.4 0 152 167 187 696.4 0 187 168 111 696.4 0 111 169 100 696.4 0 100 170 59 696.4 0 59 171 73 696.4 0 73 172 96 696.4 0 96 173 116 696.4 0 116 174 282 696.4 0 282 175 256 696.4 0 256 176 213 696.4 0 213 177 130 696.4 0 130 178 95 696.4 0 95 179 117 696.4 0 117 180 201 696.4 0 201 181 617 696.4 0 617 182 929 696.4 0 929 183 1076 696.4 0 1076 184 502 696.4 0 502 185 106 696.4 0 106 186 163 696.4 0 163 187 167 696.4 0 167 188 168 696.4 0 168 189 93 696.4 0 93 190 107 696.4 0 107 191 55 696.4 0 55 192 155 696.4 0 155 193 105 696.4 0 105 194 131 696.4 0 131 195 90 696.4 0 90 196 78 696.4 0 78 197 112 696.4 0 112 198 97 696.4 0 97 199 92 696.4 0 92 200 136 696.4 0 136 201 130 696.4 0 130 202 97 696.4 0 97 203 90 696.4 0 90 204 108 696.4 0 108 205 131 696.4 0 131 206 149 696.4 0 149 207 98 696.4 0 98 208 80 696.4 0 80 209 83 696.4 0 83 210 89 696.4 0 89 211 86 696.4 0 86 212 96 696.4 0 96 213 191 696.4 0 191 214 183 696.4 0 183 215 152 696.4 0 152 216 128 696.4 0 128 217 105 696.4 0 105 218 129 696.4 0 129 219 148 696.4 0 148 220 195 696.4 0 195 221 164 696.4 0 164 222 112 696.4 0 112 223 105 696.4 0 105 224 84 696.4 0 84 225 86 696.4 0 86 226 94 696.4 0 94 227 94 696.4 0 94 228 127 696.4 0 127 229 115 696.4 0 115 230 131 696.4 0 131 231 172 696.4 0 172 232 169 696.4 0 169 233 207 696.4 0 207 234 118 696.4 0 118 235 110 696.4 0 110 236 121 696.4 0 121 237 87 696.4 0 87 238 188 696.4 0 188 239 303 696.4 0 303 240 769 696.4 0 769 241 1042 696.4 0 1042 242 1015 696.4 0 1015 243 444 696.4 0 444 244 322 696.4 0 322 245 154 696.4 0 154 246 123 696.4 0 123 247 139 696.4 0 139 248 150 696.4 0 150 249 153 696.4 0 153 250 160 696.4 0 160 251 135 696.4 0 135 252 171 696.4 0 171 253 247 696.4 0 247 254 173 696.4 0 173 255 190 696.4 0 190 256 123 696.4 0 123 257 151 696.4 0 151 258 139 696.4 0 139 259 146 696.4 0 146 260 125 696.4 0 125 261 182 696.4 0 182 262 124 696.4 0 124 263 145 696.4 0 145 264 101 696.4 0 101 265 129 696.4 0 129 266 153 696.4 0 153 267 637 696.4 0 637 268 941 696.4 0 941 269 1284 696.4 0 1284 270 554 696.4 0 554 271 222 696.4 0 222 272 285 696.4 0 285 273 264 696.4 0 264 274 275 696.4 0 275 275 471 696.4 0 471 276 580 696.4 0 580 277 585 696.4 0 585 278 557 696.4 0 557 279 752 696.4 0 752 280 365 696.4 0 365 281 513 696.4 0 513 282 492 696.4 0 492 283 344 696.4 0 344 284 192 696.4 0 192 285 122 696.4 0 122 286 105 696.4 0 105 287 139 696.4 0 139 288 215 696.4 0 215 289 185 696.4 0 185 290 227 696.4 0 227 291 149 696.4 0 149 292 210 696.4 0 210 293 204 696.4 0 204 294 347 696.4 0 347 295 336 696.4 0 336 296 283 696.4 0 283 297 153 696.4 0 153 298 108 696.4 0 108 299 91 696.4 0 91 300 101 696.4 0 101 301 149 696.4 0 149 Finished in 9.88 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 287,291 Reads with adapters: 8,221 (2.9%) Reads that were too short: 21 (0.0%) Reads written (passing filters): 8,200 (2.9%) Total basepairs processed: 45,404,455 bp Total written (filtered): 2,224,339 bp (4.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8221 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 3 0 2 19 73 0.0 3 0 66 4 1 2 20 7772 0.0 4 7490 234 37 5 6 21 32 0.0 4 7 23 1 0 1 22 6 0.0 4 2 0 2 1 1 23 96 0.0 4 88 4 0 3 1 24 4 0.0 4 1 0 0 0 3 26 2 0.0 4 0 0 0 2 27 1 0.0 4 0 1 28 33 0.0 4 0 0 0 0 33 30 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 52 1 0.0 4 1 56 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 60 2 0.0 4 1 0 0 0 1 61 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 74 1 0.0 4 1 77 2 0.0 4 1 0 0 0 1 79 2 0.0 4 1 0 0 0 1 84 5 0.0 4 3 1 1 85 1 0.0 4 1 95 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 2 0.0 4 0 0 0 0 2 127 147 0.0 4 0 0 0 0 147 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 138 2 0.0 4 0 0 0 0 2 150 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 208 3 0.0 4 0 0 0 0 3 231 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 8,200 Reads with adapters: 1,781 (21.7%) Reads written (passing filters): 8,200 (100.0%) Total basepairs processed: 2,224,339 bp Total written (filtered): 1,801,087 bp (81.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1781 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 61.9% C: 2.2% G: 6.7% T: 29.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 35 128.1 0 35 4 2 32.0 0 2 9 9 0.0 0 1 8 10 16 0.0 1 3 13 11 8 0.0 1 0 8 12 111 0.0 1 2 109 13 3 0.0 1 0 3 15 1 0.0 1 0 1 152 2 0.0 1 1 1 210 4 0.0 1 2 2 211 1 0.0 1 0 1 260 5 0.0 1 5 261 3 0.0 1 3 262 975 0.0 1 903 72 263 84 0.0 1 76 8 265 2 0.0 1 2 267 2 0.0 1 2 268 6 0.0 1 4 2 269 470 0.0 1 446 24 270 40 0.0 1 37 3 271 2 0.0 1 1 1