This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 130.42 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 95,486 (3.2%) Reads that were too short: 16,323 (0.6%) Reads written (passing filters): 79,163 (2.7%) Total basepairs processed: 887,907,860 bp Total written (filtered): 19,050,869 bp (2.1%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 95486 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1276 720.2 0 1276 7 6029 720.2 0 6029 8 4661 720.2 0 4661 9 59 720.2 0 59 10 353 720.2 0 353 11 70 720.2 0 70 12 39 720.2 0 39 13 20 720.2 0 20 14 32 720.2 0 32 15 15 720.2 0 15 16 14 720.2 0 14 17 27 720.2 0 27 18 43 720.2 0 43 19 30 720.2 0 30 20 22 720.2 0 22 21 40 720.2 0 40 22 43 720.2 0 43 23 22 720.2 0 22 24 27 720.2 0 27 25 19 720.2 0 19 26 29 720.2 0 29 27 35 720.2 0 35 28 72 720.2 0 72 29 24 720.2 0 24 30 35 720.2 0 35 31 24 720.2 0 24 32 21 720.2 0 21 33 22 720.2 0 22 34 22 720.2 0 22 35 23 720.2 0 23 36 39 720.2 0 39 37 41 720.2 0 41 38 89 720.2 0 89 39 33 720.2 0 33 40 66 720.2 0 66 41 69 720.2 0 69 42 49 720.2 0 49 43 118 720.2 0 118 44 60 720.2 0 60 45 30 720.2 0 30 46 18 720.2 0 18 47 41 720.2 0 41 48 93 720.2 0 93 49 136 720.2 0 136 50 188 720.2 0 188 51 349 720.2 0 349 52 365 720.2 0 365 53 606 720.2 0 606 54 896 720.2 0 896 55 16194 720.2 0 16194 56 5075 720.2 0 5075 57 27952 720.2 0 27952 58 129 720.2 0 129 59 23 720.2 0 23 60 27 720.2 0 27 61 29 720.2 0 29 62 16 720.2 0 16 63 44 720.2 0 44 64 23 720.2 0 23 65 34 720.2 0 34 66 22 720.2 0 22 67 32 720.2 0 32 68 23 720.2 0 23 69 40 720.2 0 40 70 20 720.2 0 20 71 23 720.2 0 23 72 28 720.2 0 28 73 32 720.2 0 32 74 51 720.2 0 51 75 35 720.2 0 35 76 86 720.2 0 86 77 54 720.2 0 54 78 113 720.2 0 113 79 19 720.2 0 19 80 33 720.2 0 33 81 47 720.2 0 47 82 41 720.2 0 41 83 58 720.2 0 58 84 97 720.2 0 97 85 778 720.2 0 778 86 432 720.2 0 432 87 1524 720.2 0 1524 88 35 720.2 0 35 89 44 720.2 0 44 90 34 720.2 0 34 91 30 720.2 0 30 92 100 720.2 0 100 93 38 720.2 0 38 94 32 720.2 0 32 95 35 720.2 0 35 96 339 720.2 0 339 97 45 720.2 0 45 98 34 720.2 0 34 99 28 720.2 0 28 100 52 720.2 0 52 101 49 720.2 0 49 102 323 720.2 0 323 103 94 720.2 0 94 104 377 720.2 0 377 105 52 720.2 0 52 106 36 720.2 0 36 107 34 720.2 0 34 108 33 720.2 0 33 109 38 720.2 0 38 110 39 720.2 0 39 111 34 720.2 0 34 112 31 720.2 0 31 113 25 720.2 0 25 114 26 720.2 0 26 115 21 720.2 0 21 116 15 720.2 0 15 117 34 720.2 0 34 118 130 720.2 0 130 119 220 720.2 0 220 120 118 720.2 0 118 121 31 720.2 0 31 122 27 720.2 0 27 123 36 720.2 0 36 124 68 720.2 0 68 125 24 720.2 0 24 126 84 720.2 0 84 127 41 720.2 0 41 128 50 720.2 0 50 129 118 720.2 0 118 130 71 720.2 0 71 131 43 720.2 0 43 132 68 720.2 0 68 133 91 720.2 0 91 134 62 720.2 0 62 135 42 720.2 0 42 136 42 720.2 0 42 137 122 720.2 0 122 138 288 720.2 0 288 139 307 720.2 0 307 140 110 720.2 0 110 141 61 720.2 0 61 142 79 720.2 0 79 143 28 720.2 0 28 144 47 720.2 0 47 145 32 720.2 0 32 146 50 720.2 0 50 147 114 720.2 0 114 148 110 720.2 0 110 149 75 720.2 0 75 150 36 720.2 0 36 151 65 720.2 0 65 152 65 720.2 0 65 153 66 720.2 0 66 154 49 720.2 0 49 155 60 720.2 0 60 156 71 720.2 0 71 157 157 720.2 0 157 158 197 720.2 0 197 159 152 720.2 0 152 160 25 720.2 0 25 161 54 720.2 0 54 162 41 720.2 0 41 163 79 720.2 0 79 164 89 720.2 0 89 165 160 720.2 0 160 166 91 720.2 0 91 167 119 720.2 0 119 168 149 720.2 0 149 169 140 720.2 0 140 170 71 720.2 0 71 171 55 720.2 0 55 172 43 720.2 0 43 173 39 720.2 0 39 174 47 720.2 0 47 175 43 720.2 0 43 176 65 720.2 0 65 177 36 720.2 0 36 178 48 720.2 0 48 179 35 720.2 0 35 180 40 720.2 0 40 181 55 720.2 0 55 182 76 720.2 0 76 183 108 720.2 0 108 184 109 720.2 0 109 185 54 720.2 0 54 186 66 720.2 0 66 187 260 720.2 0 260 188 387 720.2 0 387 189 317 720.2 0 317 190 246 720.2 0 246 191 68 720.2 0 68 192 145 720.2 0 145 193 26 720.2 0 26 194 41 720.2 0 41 195 43 720.2 0 43 196 31 720.2 0 31 197 36 720.2 0 36 198 37 720.2 0 37 199 85 720.2 0 85 200 62 720.2 0 62 201 60 720.2 0 60 202 33 720.2 0 33 203 40 720.2 0 40 204 40 720.2 0 40 205 44 720.2 0 44 206 62 720.2 0 62 207 77 720.2 0 77 208 73 720.2 0 73 209 125 720.2 0 125 210 31 720.2 0 31 211 34 720.2 0 34 212 44 720.2 0 44 213 40 720.2 0 40 214 78 720.2 0 78 215 76 720.2 0 76 216 54 720.2 0 54 217 40 720.2 0 40 218 51 720.2 0 51 219 367 720.2 0 367 220 251 720.2 0 251 221 481 720.2 0 481 222 29 720.2 0 29 223 44 720.2 0 44 224 84 720.2 0 84 225 126 720.2 0 126 226 1207 720.2 0 1207 227 23 720.2 0 23 228 53 720.2 0 53 229 31 720.2 0 31 230 35 720.2 0 35 231 40 720.2 0 40 232 40 720.2 0 40 233 48 720.2 0 48 234 114 720.2 0 114 235 204 720.2 0 204 236 63 720.2 0 63 237 26 720.2 0 26 238 76 720.2 0 76 239 129 720.2 0 129 240 183 720.2 0 183 241 115 720.2 0 115 242 56 720.2 0 56 243 67 720.2 0 67 244 30 720.2 0 30 245 35 720.2 0 35 246 65 720.2 0 65 247 61 720.2 0 61 248 59 720.2 0 59 249 53 720.2 0 53 250 152 720.2 0 152 251 113 720.2 0 113 252 88 720.2 0 88 253 32 720.2 0 32 254 21 720.2 0 21 255 21 720.2 0 21 256 43 720.2 0 43 257 51 720.2 0 51 258 98 720.2 0 98 259 152 720.2 0 152 260 289 720.2 0 289 261 380 720.2 0 380 262 188 720.2 0 188 263 54 720.2 0 54 264 43 720.2 0 43 265 38 720.2 0 38 266 54 720.2 0 54 267 81 720.2 0 81 268 62 720.2 0 62 269 92 720.2 0 92 270 65 720.2 0 65 271 48 720.2 0 48 272 51 720.2 0 51 273 53 720.2 0 53 274 110 720.2 0 110 275 90 720.2 0 90 276 301 720.2 0 301 277 188 720.2 0 188 278 724 720.2 0 724 279 325 720.2 0 325 280 522 720.2 0 522 281 160 720.2 0 160 282 153 720.2 0 153 283 146 720.2 0 146 284 104 720.2 0 104 285 82 720.2 0 82 286 69 720.2 0 69 287 294 720.2 0 294 288 520 720.2 0 520 289 281 720.2 0 281 290 109 720.2 0 109 291 123 720.2 0 123 292 149 720.2 0 149 293 218 720.2 0 218 294 129 720.2 0 129 295 145 720.2 0 145 296 191 720.2 0 191 297 173 720.2 0 173 298 829 720.2 0 829 299 1229 720.2 0 1229 300 1433 720.2 0 1433 301 120 720.2 0 120 Finished in 5.16 s (65 us/read; 0.92 M reads/minute). === Summary === Total reads processed: 79,163 Reads with adapters: 5,973 (7.5%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 5,955 (7.5%) Total basepairs processed: 19,050,869 bp Total written (filtered): 1,615,349 bp (8.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5973 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 1 0.0 3 0 0 0 1 19 21 0.0 3 0 16 2 3 20 4726 0.0 4 4486 197 25 12 6 21 21 0.0 4 8 12 1 22 6 0.0 4 1 1 3 1 23 1075 0.0 4 1004 44 11 12 4 24 2 0.0 4 0 0 0 1 1 26 4 0.0 4 1 0 0 3 36 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 1 44 2 0.0 4 1 1 45 1 0.0 4 0 0 1 50 1 0.0 4 0 0 0 0 1 53 2 0.0 4 0 0 0 1 1 54 2 0.0 4 0 0 0 0 2 59 80 0.0 4 0 0 0 1 79 73 1 0.0 4 1 84 1 0.0 4 0 0 0 1 94 3 0.0 4 0 0 0 0 3 97 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 1 116 1 0.0 4 0 1 123 3 0.0 4 0 0 0 0 3 124 4 0.0 4 0 0 0 0 4 126 2 0.0 4 0 0 0 0 2 127 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 1 180 1 0.0 4 0 1 210 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.34 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 5,955 Reads with adapters: 1,459 (24.5%) Reads written (passing filters): 5,955 (100.0%) Total basepairs processed: 1,615,349 bp Total written (filtered): 1,599,790 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1459 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 5.8% G: 90.8% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 93.0 0 6 4 1 23.3 0 1 8 1 0.1 0 1 9 206 0.0 0 12 194 10 440 0.0 1 21 419 11 799 0.0 1 12 787 12 3 0.0 1 0 3 90 1 0.0 1 1 151 1 0.0 1 1 209 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.