This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 130.80 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 100,957 (3.4%) Reads that were too short: 12,692 (0.4%) Reads written (passing filters): 88,265 (3.0%) Total basepairs processed: 887,907,860 bp Total written (filtered): 21,679,739 bp (2.4%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 100957 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1512 720.2 0 1512 7 4792 720.2 0 4792 8 6331 720.2 0 6331 9 55 720.2 0 55 10 24 720.2 0 24 11 22 720.2 0 22 12 36 720.2 0 36 13 14 720.2 0 14 14 14 720.2 0 14 15 12 720.2 0 12 16 23 720.2 0 23 17 15 720.2 0 15 18 51 720.2 0 51 19 31 720.2 0 31 20 21 720.2 0 21 21 33 720.2 0 33 22 16 720.2 0 16 23 22 720.2 0 22 24 27 720.2 0 27 25 23 720.2 0 23 26 15 720.2 0 15 27 25 720.2 0 25 28 97 720.2 0 97 29 20 720.2 0 20 30 23 720.2 0 23 31 21 720.2 0 21 32 11 720.2 0 11 33 16 720.2 0 16 34 13 720.2 0 13 35 19 720.2 0 19 36 17 720.2 0 17 37 28 720.2 0 28 38 110 720.2 0 110 39 34 720.2 0 34 40 61 720.2 0 61 41 79 720.2 0 79 42 74 720.2 0 74 43 127 720.2 0 127 44 70 720.2 0 70 45 40 720.2 0 40 46 29 720.2 0 29 47 43 720.2 0 43 48 99 720.2 0 99 49 112 720.2 0 112 50 207 720.2 0 207 51 448 720.2 0 448 52 388 720.2 0 388 53 678 720.2 0 678 54 1003 720.2 0 1003 55 20431 720.2 0 20431 56 6138 720.2 0 6138 57 35656 720.2 0 35656 58 112 720.2 0 112 59 21 720.2 0 21 60 20 720.2 0 20 61 22 720.2 0 22 62 17 720.2 0 17 63 36 720.2 0 36 64 18 720.2 0 18 65 22 720.2 0 22 66 19 720.2 0 19 67 21 720.2 0 21 68 13 720.2 0 13 69 29 720.2 0 29 70 14 720.2 0 14 71 12 720.2 0 12 72 22 720.2 0 22 73 21 720.2 0 21 74 29 720.2 0 29 75 35 720.2 0 35 76 42 720.2 0 42 77 28 720.2 0 28 78 82 720.2 0 82 79 14 720.2 0 14 80 23 720.2 0 23 81 35 720.2 0 35 82 41 720.2 0 41 83 40 720.2 0 40 84 63 720.2 0 63 85 769 720.2 0 769 86 396 720.2 0 396 87 1551 720.2 0 1551 88 33 720.2 0 33 89 19 720.2 0 19 90 22 720.2 0 22 91 30 720.2 0 30 92 88 720.2 0 88 93 20 720.2 0 20 94 21 720.2 0 21 95 14 720.2 0 14 96 325 720.2 0 325 97 26 720.2 0 26 98 8 720.2 0 8 99 24 720.2 0 24 100 20 720.2 0 20 101 29 720.2 0 29 102 124 720.2 0 124 103 71 720.2 0 71 104 171 720.2 0 171 105 16 720.2 0 16 106 30 720.2 0 30 107 21 720.2 0 21 108 28 720.2 0 28 109 13 720.2 0 13 110 35 720.2 0 35 111 26 720.2 0 26 112 26 720.2 0 26 113 17 720.2 0 17 114 17 720.2 0 17 115 13 720.2 0 13 116 10 720.2 0 10 117 28 720.2 0 28 118 85 720.2 0 85 119 188 720.2 0 188 120 95 720.2 0 95 121 18 720.2 0 18 122 17 720.2 0 17 123 17 720.2 0 17 124 22 720.2 0 22 125 22 720.2 0 22 126 53 720.2 0 53 127 19 720.2 0 19 128 21 720.2 0 21 129 106 720.2 0 106 130 40 720.2 0 40 131 24 720.2 0 24 132 28 720.2 0 28 133 36 720.2 0 36 134 24 720.2 0 24 135 25 720.2 0 25 136 17 720.2 0 17 137 46 720.2 0 46 138 87 720.2 0 87 139 113 720.2 0 113 140 39 720.2 0 39 141 24 720.2 0 24 142 30 720.2 0 30 143 14 720.2 0 14 144 23 720.2 0 23 145 24 720.2 0 24 146 28 720.2 0 28 147 102 720.2 0 102 148 55 720.2 0 55 149 49 720.2 0 49 150 24 720.2 0 24 151 49 720.2 0 49 152 20 720.2 0 20 153 25 720.2 0 25 154 35 720.2 0 35 155 47 720.2 0 47 156 52 720.2 0 52 157 96 720.2 0 96 158 101 720.2 0 101 159 107 720.2 0 107 160 29 720.2 0 29 161 50 720.2 0 50 162 25 720.2 0 25 163 46 720.2 0 46 164 45 720.2 0 45 165 69 720.2 0 69 166 56 720.2 0 56 167 61 720.2 0 61 168 82 720.2 0 82 169 57 720.2 0 57 170 31 720.2 0 31 171 19 720.2 0 19 172 52 720.2 0 52 173 43 720.2 0 43 174 36 720.2 0 36 175 19 720.2 0 19 176 54 720.2 0 54 177 24 720.2 0 24 178 24 720.2 0 24 179 10 720.2 0 10 180 18 720.2 0 18 181 23 720.2 0 23 182 28 720.2 0 28 183 48 720.2 0 48 184 43 720.2 0 43 185 26 720.2 0 26 186 30 720.2 0 30 187 240 720.2 0 240 188 149 720.2 0 149 189 173 720.2 0 173 190 141 720.2 0 141 191 40 720.2 0 40 192 108 720.2 0 108 193 11 720.2 0 11 194 17 720.2 0 17 195 32 720.2 0 32 196 13 720.2 0 13 197 9 720.2 0 9 198 19 720.2 0 19 199 55 720.2 0 55 200 32 720.2 0 32 201 33 720.2 0 33 202 22 720.2 0 22 203 24 720.2 0 24 204 20 720.2 0 20 205 20 720.2 0 20 206 27 720.2 0 27 207 35 720.2 0 35 208 52 720.2 0 52 209 139 720.2 0 139 210 23 720.2 0 23 211 17 720.2 0 17 212 20 720.2 0 20 213 21 720.2 0 21 214 38 720.2 0 38 215 33 720.2 0 33 216 26 720.2 0 26 217 15 720.2 0 15 218 28 720.2 0 28 219 186 720.2 0 186 220 152 720.2 0 152 221 295 720.2 0 295 222 23 720.2 0 23 223 27 720.2 0 27 224 55 720.2 0 55 225 51 720.2 0 51 226 1369 720.2 0 1369 227 15 720.2 0 15 228 18 720.2 0 18 229 25 720.2 0 25 230 18 720.2 0 18 231 24 720.2 0 24 232 19 720.2 0 19 233 39 720.2 0 39 234 19 720.2 0 19 235 42 720.2 0 42 236 23 720.2 0 23 237 17 720.2 0 17 238 23 720.2 0 23 239 48 720.2 0 48 240 80 720.2 0 80 241 68 720.2 0 68 242 27 720.2 0 27 243 23 720.2 0 23 244 15 720.2 0 15 245 14 720.2 0 14 246 37 720.2 0 37 247 29 720.2 0 29 248 30 720.2 0 30 249 32 720.2 0 32 250 60 720.2 0 60 251 47 720.2 0 47 252 39 720.2 0 39 253 18 720.2 0 18 254 19 720.2 0 19 255 12 720.2 0 12 256 14 720.2 0 14 257 21 720.2 0 21 258 46 720.2 0 46 259 76 720.2 0 76 260 139 720.2 0 139 261 138 720.2 0 138 262 75 720.2 0 75 263 27 720.2 0 27 264 23 720.2 0 23 265 32 720.2 0 32 266 23 720.2 0 23 267 28 720.2 0 28 268 35 720.2 0 35 269 28 720.2 0 28 270 28 720.2 0 28 271 24 720.2 0 24 272 45 720.2 0 45 273 32 720.2 0 32 274 60 720.2 0 60 275 60 720.2 0 60 276 539 720.2 0 539 277 215 720.2 0 215 278 1166 720.2 0 1166 279 195 720.2 0 195 280 430 720.2 0 430 281 68 720.2 0 68 282 57 720.2 0 57 283 49 720.2 0 49 284 69 720.2 0 69 285 55 720.2 0 55 286 43 720.2 0 43 287 137 720.2 0 137 288 247 720.2 0 247 289 109 720.2 0 109 290 63 720.2 0 63 291 78 720.2 0 78 292 53 720.2 0 53 293 55 720.2 0 55 294 68 720.2 0 68 295 90 720.2 0 90 296 103 720.2 0 103 297 90 720.2 0 90 298 946 720.2 0 946 299 1473 720.2 0 1473 300 1699 720.2 0 1699 301 43 720.2 0 43 Finished in 5.38 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 88,265 Reads with adapters: 4,795 (5.4%) Reads that were too short: 28 (0.0%) Reads written (passing filters): 4,767 (5.4%) Total basepairs processed: 21,679,739 bp Total written (filtered): 1,316,856 bp (6.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4795 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 17 0.0 3 0 16 1 17 4704 0.0 3 4624 78 1 1 18 10 0.0 3 2 5 3 19 3 0.0 3 1 0 2 20 5 0.0 3 0 0 0 5 21 8 0.0 3 0 0 0 8 28 1 0.0 3 0 0 0 1 30 4 0.0 3 1 0 0 3 32 1 0.0 3 0 0 0 1 34 3 0.0 3 0 0 1 2 37 1 0.0 3 0 0 0 1 42 1 0.0 3 1 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 67 2 0.0 3 0 0 0 2 72 1 0.0 3 1 73 1 0.0 3 0 0 0 1 78 1 0.0 3 1 81 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 111 1 0.0 3 1 115 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 232 2 0.0 3 0 0 0 2 233 4 0.0 3 0 0 0 4 234 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.25 s (52 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 4,767 Reads with adapters: 19 (0.4%) Reads written (passing filters): 4,767 (100.0%) Total basepairs processed: 1,316,856 bp Total written (filtered): 1,316,790 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 19 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 21.1% C: 26.3% G: 52.6% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 10 74.5 0 10 4 9 18.6 0 9