This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 128.29 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 2,949,860 Reads with adapters: 77,675 (2.6%) Reads that were too short: 16,108 (0.5%) Reads written (passing filters): 61,567 (2.1%) Total basepairs processed: 887,907,860 bp Total written (filtered): 10,811,030 bp (1.2%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 77675 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 106 720.2 0 106 7 1329 720.2 0 1329 8 1149 720.2 0 1149 9 74 720.2 0 74 10 61 720.2 0 61 11 37 720.2 0 37 12 47 720.2 0 47 13 44 720.2 0 44 14 60 720.2 0 60 15 56 720.2 0 56 16 44 720.2 0 44 17 78 720.2 0 78 18 59 720.2 0 59 19 27 720.2 0 27 20 55 720.2 0 55 21 63 720.2 0 63 22 43 720.2 0 43 23 61 720.2 0 61 24 51 720.2 0 51 25 62 720.2 0 62 26 73 720.2 0 73 27 206 720.2 0 206 28 342 720.2 0 342 29 174 720.2 0 174 30 55 720.2 0 55 31 56 720.2 0 56 32 56 720.2 0 56 33 60 720.2 0 60 34 72 720.2 0 72 35 100 720.2 0 100 36 434 720.2 0 434 37 82 720.2 0 82 38 56 720.2 0 56 39 50 720.2 0 50 40 37 720.2 0 37 41 91 720.2 0 91 42 59 720.2 0 59 43 46 720.2 0 46 44 70 720.2 0 70 45 55 720.2 0 55 46 49 720.2 0 49 47 53 720.2 0 53 48 46 720.2 0 46 49 48 720.2 0 48 50 55 720.2 0 55 51 63 720.2 0 63 52 78 720.2 0 78 53 52 720.2 0 52 54 58 720.2 0 58 55 63 720.2 0 63 56 79 720.2 0 79 57 75 720.2 0 75 58 295 720.2 0 295 59 240 720.2 0 240 60 369 720.2 0 369 61 54 720.2 0 54 62 52 720.2 0 52 63 80 720.2 0 80 64 38 720.2 0 38 65 52 720.2 0 52 66 56 720.2 0 56 67 61 720.2 0 61 68 80 720.2 0 80 69 133 720.2 0 133 70 359 720.2 0 359 71 141 720.2 0 141 72 1178 720.2 0 1178 73 266 720.2 0 266 74 1335 720.2 0 1335 75 77 720.2 0 77 76 54 720.2 0 54 77 61 720.2 0 61 78 57 720.2 0 57 79 45 720.2 0 45 80 67 720.2 0 67 81 53 720.2 0 53 82 51 720.2 0 51 83 44 720.2 0 44 84 39 720.2 0 39 85 42 720.2 0 42 86 40 720.2 0 40 87 50 720.2 0 50 88 86 720.2 0 86 89 47 720.2 0 47 90 126 720.2 0 126 91 139 720.2 0 139 92 122 720.2 0 122 93 53 720.2 0 53 94 75 720.2 0 75 95 56 720.2 0 56 96 111 720.2 0 111 97 47 720.2 0 47 98 53 720.2 0 53 99 67 720.2 0 67 100 47 720.2 0 47 101 58 720.2 0 58 102 45 720.2 0 45 103 49 720.2 0 49 104 44 720.2 0 44 105 46 720.2 0 46 106 62 720.2 0 62 107 54 720.2 0 54 108 75 720.2 0 75 109 57 720.2 0 57 110 85 720.2 0 85 111 64 720.2 0 64 112 197 720.2 0 197 113 300 720.2 0 300 114 324 720.2 0 324 115 57 720.2 0 57 116 50 720.2 0 50 117 51 720.2 0 51 118 41 720.2 0 41 119 118 720.2 0 118 120 71 720.2 0 71 121 88 720.2 0 88 122 75 720.2 0 75 123 75 720.2 0 75 124 74 720.2 0 74 125 318 720.2 0 318 126 85 720.2 0 85 127 104 720.2 0 104 128 165 720.2 0 165 129 249 720.2 0 249 130 491 720.2 0 491 131 129 720.2 0 129 132 144 720.2 0 144 133 220 720.2 0 220 134 146 720.2 0 146 135 154 720.2 0 154 136 201 720.2 0 201 137 145 720.2 0 145 138 183 720.2 0 183 139 255 720.2 0 255 140 283 720.2 0 283 141 1606 720.2 0 1606 142 586 720.2 0 586 143 7785 720.2 0 7785 144 11522 720.2 0 11522 145 11449 720.2 0 11449 146 181 720.2 0 181 147 84 720.2 0 84 148 117 720.2 0 117 149 97 720.2 0 97 150 80 720.2 0 80 151 70 720.2 0 70 152 94 720.2 0 94 153 71 720.2 0 71 154 87 720.2 0 87 155 394 720.2 0 394 156 1688 720.2 0 1688 157 3429 720.2 0 3429 158 91 720.2 0 91 159 59 720.2 0 59 160 55 720.2 0 55 161 74 720.2 0 74 162 145 720.2 0 145 163 484 720.2 0 484 164 788 720.2 0 788 165 178 720.2 0 178 166 50 720.2 0 50 167 56 720.2 0 56 168 76 720.2 0 76 169 57 720.2 0 57 170 65 720.2 0 65 171 51 720.2 0 51 172 48 720.2 0 48 173 52 720.2 0 52 174 41 720.2 0 41 175 47 720.2 0 47 176 59 720.2 0 59 177 49 720.2 0 49 178 46 720.2 0 46 179 55 720.2 0 55 180 52 720.2 0 52 181 41 720.2 0 41 182 50 720.2 0 50 183 47 720.2 0 47 184 52 720.2 0 52 185 41 720.2 0 41 186 49 720.2 0 49 187 54 720.2 0 54 188 101 720.2 0 101 189 60 720.2 0 60 190 55 720.2 0 55 191 56 720.2 0 56 192 57 720.2 0 57 193 56 720.2 0 56 194 52 720.2 0 52 195 51 720.2 0 51 196 74 720.2 0 74 197 71 720.2 0 71 198 61 720.2 0 61 199 65 720.2 0 65 200 53 720.2 0 53 201 74 720.2 0 74 202 52 720.2 0 52 203 84 720.2 0 84 204 63 720.2 0 63 205 67 720.2 0 67 206 83 720.2 0 83 207 68 720.2 0 68 208 94 720.2 0 94 209 115 720.2 0 115 210 166 720.2 0 166 211 250 720.2 0 250 212 2893 720.2 0 2893 213 138 720.2 0 138 214 151 720.2 0 151 215 808 720.2 0 808 216 559 720.2 0 559 217 2933 720.2 0 2933 218 66 720.2 0 66 219 44 720.2 0 44 220 49 720.2 0 49 221 64 720.2 0 64 222 78 720.2 0 78 223 93 720.2 0 93 224 71 720.2 0 71 225 72 720.2 0 72 226 64 720.2 0 64 227 68 720.2 0 68 228 84 720.2 0 84 229 69 720.2 0 69 230 63 720.2 0 63 231 111 720.2 0 111 232 167 720.2 0 167 233 187 720.2 0 187 234 103 720.2 0 103 235 105 720.2 0 105 236 93 720.2 0 93 237 67 720.2 0 67 238 50 720.2 0 50 239 79 720.2 0 79 240 53 720.2 0 53 241 44 720.2 0 44 242 62 720.2 0 62 243 57 720.2 0 57 244 80 720.2 0 80 245 119 720.2 0 119 246 88 720.2 0 88 247 38 720.2 0 38 248 50 720.2 0 50 249 31 720.2 0 31 250 40 720.2 0 40 251 53 720.2 0 53 252 56 720.2 0 56 253 74 720.2 0 74 254 105 720.2 0 105 255 81 720.2 0 81 256 62 720.2 0 62 257 57 720.2 0 57 258 64 720.2 0 64 259 83 720.2 0 83 260 84 720.2 0 84 261 65 720.2 0 65 262 43 720.2 0 43 263 112 720.2 0 112 264 133 720.2 0 133 265 156 720.2 0 156 266 113 720.2 0 113 267 50 720.2 0 50 268 63 720.2 0 63 269 79 720.2 0 79 270 72 720.2 0 72 271 69 720.2 0 69 272 79 720.2 0 79 273 62 720.2 0 62 274 48 720.2 0 48 275 55 720.2 0 55 276 76 720.2 0 76 277 53 720.2 0 53 278 79 720.2 0 79 279 75 720.2 0 75 280 110 720.2 0 110 281 105 720.2 0 105 282 132 720.2 0 132 283 82 720.2 0 82 284 122 720.2 0 122 285 104 720.2 0 104 286 108 720.2 0 108 287 99 720.2 0 99 288 60 720.2 0 60 289 63 720.2 0 63 290 80 720.2 0 80 291 91 720.2 0 91 292 96 720.2 0 96 293 104 720.2 0 104 294 111 720.2 0 111 295 257 720.2 0 257 296 261 720.2 0 261 297 522 720.2 0 522 298 129 720.2 0 129 299 80 720.2 0 80 300 69 720.2 0 69 301 89 720.2 0 89 Finished in 3.47 s (56 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 61,567 Reads with adapters: 1,054 (1.7%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 1,035 (1.7%) Total basepairs processed: 10,811,030 bp Total written (filtered): 281,546 bp (2.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1054 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 19 0.0 3 0 18 1 20 995 0.0 4 934 47 10 4 21 8 0.0 4 2 5 0 0 1 23 4 0.0 4 1 0 0 1 2 46 1 0.0 4 0 0 0 0 1 52 1 0.0 4 1 54 2 0.0 4 0 2 87 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 109 2 0.0 4 0 0 0 0 2 120 1 0.0 4 0 1 124 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 146 5 0.0 4 0 0 0 0 5 147 2 0.0 4 0 0 0 0 2 149 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 1 231 1 0.0 4 0 0 0 1 232 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (68 us/read; 0.89 M reads/minute). === Summary === Total reads processed: 1,035 Reads with adapters: 316 (30.5%) Reads written (passing filters): 1,035 (100.0%) Total basepairs processed: 281,546 bp Total written (filtered): 255,797 bp (90.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 316 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 23.1% C: 7.0% G: 68.0% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 16.2 0 1 10 2 0.0 1 0 2 11 223 0.0 1 8 215 12 4 0.0 1 0 4 269 1 0.0 1 1 270 80 0.0 1 72 8 271 4 0.0 1 2 2 272 1 0.0 1 1