This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCACT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.41 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 235,507 (8.9%) Reads that were too short: 23,856 (0.9%) Reads written (passing filters): 211,651 (8.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 39,905,616 bp (5.0%) === Adapter 1 === Sequence: AGCACT; Type: regular 5'; Length: 6; Trimmed: 235507 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 208 649.0 0 208 7 7922 649.0 0 7922 8 7166 649.0 0 7166 9 51 649.0 0 51 10 30 649.0 0 30 11 34 649.0 0 34 12 171 649.0 0 171 13 145 649.0 0 145 14 129 649.0 0 129 15 41 649.0 0 41 16 40 649.0 0 40 17 33 649.0 0 33 18 35 649.0 0 35 19 36 649.0 0 36 20 34 649.0 0 34 21 35 649.0 0 35 22 50 649.0 0 50 23 47 649.0 0 47 24 37 649.0 0 37 25 405 649.0 0 405 26 175 649.0 0 175 27 97 649.0 0 97 28 62 649.0 0 62 29 88 649.0 0 88 30 62 649.0 0 62 31 37 649.0 0 37 32 70 649.0 0 70 33 51 649.0 0 51 34 38 649.0 0 38 35 114 649.0 0 114 36 74 649.0 0 74 37 60 649.0 0 60 38 41 649.0 0 41 39 48 649.0 0 48 40 58 649.0 0 58 41 47 649.0 0 47 42 71 649.0 0 71 43 83 649.0 0 83 44 70 649.0 0 70 45 35 649.0 0 35 46 57 649.0 0 57 47 83 649.0 0 83 48 58 649.0 0 58 49 74 649.0 0 74 50 177 649.0 0 177 51 66 649.0 0 66 52 50 649.0 0 50 53 64 649.0 0 64 54 93 649.0 0 93 55 80 649.0 0 80 56 120 649.0 0 120 57 67 649.0 0 67 58 81 649.0 0 81 59 58 649.0 0 58 60 62 649.0 0 62 61 81 649.0 0 81 62 99 649.0 0 99 63 74 649.0 0 74 64 89 649.0 0 89 65 479 649.0 0 479 66 289 649.0 0 289 67 324 649.0 0 324 68 78 649.0 0 78 69 60 649.0 0 60 70 63 649.0 0 63 71 55 649.0 0 55 72 88 649.0 0 88 73 75 649.0 0 75 74 62 649.0 0 62 75 55 649.0 0 55 76 50 649.0 0 50 77 85 649.0 0 85 78 78 649.0 0 78 79 183 649.0 0 183 80 144 649.0 0 144 81 148 649.0 0 148 82 122 649.0 0 122 83 105 649.0 0 105 84 75 649.0 0 75 85 72 649.0 0 72 86 151 649.0 0 151 87 158 649.0 0 158 88 212 649.0 0 212 89 283 649.0 0 283 90 414 649.0 0 414 91 779 649.0 0 779 92 318 649.0 0 318 93 402 649.0 0 402 94 522 649.0 0 522 95 292 649.0 0 292 96 296 649.0 0 296 97 404 649.0 0 404 98 442 649.0 0 442 99 599 649.0 0 599 100 554 649.0 0 554 101 911 649.0 0 911 102 1370 649.0 0 1370 103 3114 649.0 0 3114 104 51355 649.0 0 51355 105 30461 649.0 0 30461 106 30481 649.0 0 30481 107 181 649.0 0 181 108 51 649.0 0 51 109 55 649.0 0 55 110 72 649.0 0 72 111 84 649.0 0 84 112 101 649.0 0 101 113 114 649.0 0 114 114 155 649.0 0 155 115 763 649.0 0 763 116 406 649.0 0 406 117 139 649.0 0 139 118 101 649.0 0 101 119 209 649.0 0 209 120 116 649.0 0 116 121 160 649.0 0 160 122 69 649.0 0 69 123 100 649.0 0 100 124 211 649.0 0 211 125 189 649.0 0 189 126 302 649.0 0 302 127 168 649.0 0 168 128 147 649.0 0 147 129 92 649.0 0 92 130 115 649.0 0 115 131 106 649.0 0 106 132 133 649.0 0 133 133 123 649.0 0 123 134 118 649.0 0 118 135 72 649.0 0 72 136 98 649.0 0 98 137 67 649.0 0 67 138 187 649.0 0 187 139 191 649.0 0 191 140 192 649.0 0 192 141 133 649.0 0 133 142 173 649.0 0 173 143 138 649.0 0 138 144 290 649.0 0 290 145 167 649.0 0 167 146 418 649.0 0 418 147 207 649.0 0 207 148 142 649.0 0 142 149 160 649.0 0 160 150 98 649.0 0 98 151 213 649.0 0 213 152 283 649.0 0 283 153 364 649.0 0 364 154 299 649.0 0 299 155 454 649.0 0 454 156 479 649.0 0 479 157 915 649.0 0 915 158 1541 649.0 0 1541 159 19532 649.0 0 19532 160 11952 649.0 0 11952 161 15398 649.0 0 15398 162 146 649.0 0 146 163 106 649.0 0 106 164 82 649.0 0 82 165 103 649.0 0 103 166 113 649.0 0 113 167 239 649.0 0 239 168 154 649.0 0 154 169 191 649.0 0 191 170 76 649.0 0 76 171 116 649.0 0 116 172 214 649.0 0 214 173 231 649.0 0 231 174 184 649.0 0 184 175 101 649.0 0 101 176 82 649.0 0 82 177 138 649.0 0 138 178 106 649.0 0 106 179 105 649.0 0 105 180 228 649.0 0 228 181 159 649.0 0 159 182 129 649.0 0 129 183 134 649.0 0 134 184 325 649.0 0 325 185 207 649.0 0 207 186 288 649.0 0 288 187 115 649.0 0 115 188 175 649.0 0 175 189 200 649.0 0 200 190 161 649.0 0 161 191 95 649.0 0 95 192 107 649.0 0 107 193 105 649.0 0 105 194 119 649.0 0 119 195 127 649.0 0 127 196 221 649.0 0 221 197 195 649.0 0 195 198 190 649.0 0 190 199 251 649.0 0 251 200 618 649.0 0 618 201 501 649.0 0 501 202 442 649.0 0 442 203 115 649.0 0 115 204 155 649.0 0 155 205 292 649.0 0 292 206 219 649.0 0 219 207 283 649.0 0 283 208 187 649.0 0 187 209 222 649.0 0 222 210 91 649.0 0 91 211 94 649.0 0 94 212 96 649.0 0 96 213 126 649.0 0 126 214 134 649.0 0 134 215 128 649.0 0 128 216 139 649.0 0 139 217 115 649.0 0 115 218 203 649.0 0 203 219 259 649.0 0 259 220 231 649.0 0 231 221 187 649.0 0 187 222 156 649.0 0 156 223 231 649.0 0 231 224 118 649.0 0 118 225 109 649.0 0 109 226 169 649.0 0 169 227 162 649.0 0 162 228 241 649.0 0 241 229 211 649.0 0 211 230 187 649.0 0 187 231 171 649.0 0 171 232 126 649.0 0 126 233 109 649.0 0 109 234 111 649.0 0 111 235 103 649.0 0 103 236 146 649.0 0 146 237 232 649.0 0 232 238 401 649.0 0 401 239 389 649.0 0 389 240 1032 649.0 0 1032 241 1163 649.0 0 1163 242 991 649.0 0 991 243 440 649.0 0 440 244 114 649.0 0 114 245 114 649.0 0 114 246 147 649.0 0 147 247 126 649.0 0 126 248 148 649.0 0 148 249 138 649.0 0 138 250 127 649.0 0 127 251 157 649.0 0 157 252 166 649.0 0 166 253 147 649.0 0 147 254 241 649.0 0 241 255 316 649.0 0 316 256 303 649.0 0 303 257 246 649.0 0 246 258 161 649.0 0 161 259 133 649.0 0 133 260 86 649.0 0 86 261 106 649.0 0 106 262 110 649.0 0 110 263 136 649.0 0 136 264 100 649.0 0 100 265 137 649.0 0 137 266 157 649.0 0 157 267 205 649.0 0 205 268 229 649.0 0 229 269 233 649.0 0 233 270 208 649.0 0 208 271 131 649.0 0 131 272 151 649.0 0 151 273 188 649.0 0 188 274 248 649.0 0 248 275 245 649.0 0 245 276 233 649.0 0 233 277 203 649.0 0 203 278 679 649.0 0 679 279 447 649.0 0 447 280 578 649.0 0 578 281 685 649.0 0 685 282 549 649.0 0 549 283 736 649.0 0 736 284 198 649.0 0 198 285 148 649.0 0 148 286 105 649.0 0 105 287 151 649.0 0 151 288 183 649.0 0 183 289 168 649.0 0 168 290 157 649.0 0 157 291 171 649.0 0 171 292 217 649.0 0 217 293 200 649.0 0 200 294 252 649.0 0 252 295 232 649.0 0 232 296 193 649.0 0 193 297 131 649.0 0 131 298 127 649.0 0 127 299 262 649.0 0 262 300 398 649.0 0 398 301 283 649.0 0 283 Finished in 8.77 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 211,651 Reads with adapters: 23,736 (11.2%) Reads that were too short: 61 (0.0%) Reads written (passing filters): 23,675 (11.2%) Total basepairs processed: 39,905,616 bp Total written (filtered): 4,729,670 bp (11.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 23736 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 1 1 19 87 0.0 3 0 68 10 5 4 20 7197 0.0 4 6828 305 49 12 3 21 26 0.0 4 4 21 1 22 3 0.0 4 0 0 1 1 1 23 23 0.0 4 3 1 0 12 7 27 3 0.0 4 1 0 0 0 2 29 5 0.0 4 0 0 0 0 5 30 15521 0.0 4 0 0 0 3 15518 31 1 0.0 4 0 0 0 0 1 33 27 0.0 4 0 0 0 0 27 36 1 0.0 4 0 0 0 0 1 40 1 0.0 4 1 49 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 56 2 0.0 4 0 0 0 0 2 69 2 0.0 4 0 0 0 0 2 73 2 0.0 4 0 1 0 0 1 86 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 1 92 1 0.0 4 0 0 0 0 1 100 1 0.0 4 1 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 1 103 1 0.0 4 0 0 0 0 1 109 3 0.0 4 0 0 0 0 3 110 2 0.0 4 0 0 0 0 2 112 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 754 0.0 4 0 0 0 0 754 128 1 0.0 4 0 1 129 2 0.0 4 0 0 0 0 2 130 2 0.0 4 0 0 0 0 2 132 1 0.0 4 0 0 0 0 1 136 2 0.0 4 0 0 0 0 2 137 5 0.0 4 0 0 1 1 3 138 5 0.0 4 0 0 0 0 5 139 2 0.0 4 0 0 0 0 2 140 2 0.0 4 0 0 0 0 2 142 2 0.0 4 0 1 1 143 2 0.0 4 0 0 1 0 1 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 154 2 0.0 4 0 0 0 0 2 164 1 0.0 4 0 0 0 0 1 167 3 0.0 4 0 0 0 0 3 185 5 0.0 4 0 0 0 0 5 187 2 0.0 4 0 0 0 2 188 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 1 191 2 0.0 4 0 0 0 0 2 195 2 0.0 4 0 0 0 0 2 198 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.68 s (29 us/read; 2.09 M reads/minute). === Summary === Total reads processed: 23,675 Reads with adapters: 1,810 (7.6%) Reads written (passing filters): 23,675 (100.0%) Total basepairs processed: 4,729,670 bp Total written (filtered): 4,711,089 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1810 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.4% C: 5.3% G: 83.2% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 55 369.9 0 55 4 94 92.5 0 94 5 11 23.1 0 11 8 1 0.4 0 1 9 5 0.1 0 0 5 10 318 0.0 1 17 301 11 1319 0.0 1 40 1279 12 5 0.0 1 0 5 13 1 0.0 1 0 1 170 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.