This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.08 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 244,803 (9.2%) Reads that were too short: 24,890 (0.9%) Reads written (passing filters): 219,913 (8.3%) Total basepairs processed: 800,111,879 bp Total written (filtered): 42,386,547 bp (5.3%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 244803 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 504 649.0 0 504 7 15176 649.0 0 15176 8 12844 649.0 0 12844 9 66 649.0 0 66 10 33 649.0 0 33 11 54 649.0 0 54 12 62 649.0 0 62 13 40 649.0 0 40 14 35 649.0 0 35 15 43 649.0 0 43 16 44 649.0 0 44 17 42 649.0 0 42 18 33 649.0 0 33 19 29 649.0 0 29 20 50 649.0 0 50 21 34 649.0 0 34 22 39 649.0 0 39 23 38 649.0 0 38 24 37 649.0 0 37 25 41 649.0 0 41 26 41 649.0 0 41 27 62 649.0 0 62 28 57 649.0 0 57 29 138 649.0 0 138 30 80 649.0 0 80 31 76 649.0 0 76 32 37 649.0 0 37 33 49 649.0 0 49 34 43 649.0 0 43 35 45 649.0 0 45 36 56 649.0 0 56 37 60 649.0 0 60 38 117 649.0 0 117 39 61 649.0 0 61 40 53 649.0 0 53 41 37 649.0 0 37 42 39 649.0 0 39 43 45 649.0 0 45 44 57 649.0 0 57 45 68 649.0 0 68 46 47 649.0 0 47 47 38 649.0 0 38 48 54 649.0 0 54 49 69 649.0 0 69 50 52 649.0 0 52 51 47 649.0 0 47 52 46 649.0 0 46 53 52 649.0 0 52 54 46 649.0 0 46 55 57 649.0 0 57 56 71 649.0 0 71 57 68 649.0 0 68 58 60 649.0 0 60 59 89 649.0 0 89 60 66 649.0 0 66 61 102 649.0 0 102 62 68 649.0 0 68 63 85 649.0 0 85 64 180 649.0 0 180 65 81 649.0 0 81 66 90 649.0 0 90 67 90 649.0 0 90 68 133 649.0 0 133 69 165 649.0 0 165 70 2126 649.0 0 2126 71 362 649.0 0 362 72 1015 649.0 0 1015 73 101 649.0 0 101 74 81 649.0 0 81 75 74 649.0 0 74 76 166 649.0 0 166 77 103 649.0 0 103 78 183 649.0 0 183 79 188 649.0 0 188 80 138 649.0 0 138 81 227 649.0 0 227 82 525 649.0 0 525 83 1551 649.0 0 1551 84 191 649.0 0 191 85 138 649.0 0 138 86 92 649.0 0 92 87 73 649.0 0 73 88 79 649.0 0 79 89 57 649.0 0 57 90 57 649.0 0 57 91 67 649.0 0 67 92 95 649.0 0 95 93 88 649.0 0 88 94 77 649.0 0 77 95 80 649.0 0 80 96 100 649.0 0 100 97 146 649.0 0 146 98 190 649.0 0 190 99 277 649.0 0 277 100 241 649.0 0 241 101 124 649.0 0 124 102 121 649.0 0 121 103 147 649.0 0 147 104 120 649.0 0 120 105 106 649.0 0 106 106 108 649.0 0 108 107 215 649.0 0 215 108 233 649.0 0 233 109 256 649.0 0 256 110 449 649.0 0 449 111 1001 649.0 0 1001 112 349 649.0 0 349 113 431 649.0 0 431 114 673 649.0 0 673 115 392 649.0 0 392 116 381 649.0 0 381 117 639 649.0 0 639 118 663 649.0 0 663 119 832 649.0 0 832 120 863 649.0 0 863 121 1374 649.0 0 1374 122 1704 649.0 0 1704 123 3105 649.0 0 3105 124 65305 649.0 0 65305 125 23461 649.0 0 23461 126 59129 649.0 0 59129 127 1551 649.0 0 1551 128 160 649.0 0 160 129 186 649.0 0 186 130 146 649.0 0 146 131 136 649.0 0 136 132 98 649.0 0 98 133 111 649.0 0 111 134 125 649.0 0 125 135 150 649.0 0 150 136 199 649.0 0 199 137 145 649.0 0 145 138 149 649.0 0 149 139 228 649.0 0 228 140 426 649.0 0 426 141 275 649.0 0 275 142 739 649.0 0 739 143 125 649.0 0 125 144 164 649.0 0 164 145 180 649.0 0 180 146 150 649.0 0 150 147 157 649.0 0 157 148 198 649.0 0 198 149 1777 649.0 0 1777 150 941 649.0 0 941 151 1578 649.0 0 1578 152 129 649.0 0 129 153 98 649.0 0 98 154 121 649.0 0 121 155 121 649.0 0 121 156 149 649.0 0 149 157 156 649.0 0 156 158 155 649.0 0 155 159 132 649.0 0 132 160 129 649.0 0 129 161 102 649.0 0 102 162 163 649.0 0 163 163 168 649.0 0 168 164 237 649.0 0 237 165 161 649.0 0 161 166 129 649.0 0 129 167 94 649.0 0 94 168 90 649.0 0 90 169 133 649.0 0 133 170 87 649.0 0 87 171 105 649.0 0 105 172 94 649.0 0 94 173 75 649.0 0 75 174 89 649.0 0 89 175 100 649.0 0 100 176 94 649.0 0 94 177 115 649.0 0 115 178 111 649.0 0 111 179 114 649.0 0 114 180 107 649.0 0 107 181 277 649.0 0 277 182 217 649.0 0 217 183 201 649.0 0 201 184 103 649.0 0 103 185 118 649.0 0 118 186 126 649.0 0 126 187 132 649.0 0 132 188 224 649.0 0 224 189 115 649.0 0 115 190 174 649.0 0 174 191 89 649.0 0 89 192 84 649.0 0 84 193 94 649.0 0 94 194 157 649.0 0 157 195 96 649.0 0 96 196 113 649.0 0 113 197 111 649.0 0 111 198 154 649.0 0 154 199 93 649.0 0 93 200 94 649.0 0 94 201 99 649.0 0 99 202 96 649.0 0 96 203 190 649.0 0 190 204 150 649.0 0 150 205 200 649.0 0 200 206 150 649.0 0 150 207 159 649.0 0 159 208 152 649.0 0 152 209 139 649.0 0 139 210 111 649.0 0 111 211 126 649.0 0 126 212 136 649.0 0 136 213 118 649.0 0 118 214 147 649.0 0 147 215 113 649.0 0 113 216 127 649.0 0 127 217 210 649.0 0 210 218 172 649.0 0 172 219 169 649.0 0 169 220 169 649.0 0 169 221 183 649.0 0 183 222 237 649.0 0 237 223 981 649.0 0 981 224 1109 649.0 0 1109 225 3496 649.0 0 3496 226 3751 649.0 0 3751 227 3229 649.0 0 3229 228 132 649.0 0 132 229 109 649.0 0 109 230 106 649.0 0 106 231 92 649.0 0 92 232 113 649.0 0 113 233 112 649.0 0 112 234 142 649.0 0 142 235 151 649.0 0 151 236 141 649.0 0 141 237 114 649.0 0 114 238 133 649.0 0 133 239 128 649.0 0 128 240 147 649.0 0 147 241 115 649.0 0 115 242 132 649.0 0 132 243 196 649.0 0 196 244 194 649.0 0 194 245 191 649.0 0 191 246 134 649.0 0 134 247 78 649.0 0 78 248 82 649.0 0 82 249 99 649.0 0 99 250 84 649.0 0 84 251 106 649.0 0 106 252 114 649.0 0 114 253 147 649.0 0 147 254 119 649.0 0 119 255 171 649.0 0 171 256 144 649.0 0 144 257 130 649.0 0 130 258 168 649.0 0 168 259 123 649.0 0 123 260 130 649.0 0 130 261 135 649.0 0 135 262 128 649.0 0 128 263 205 649.0 0 205 264 157 649.0 0 157 265 167 649.0 0 167 266 149 649.0 0 149 267 153 649.0 0 153 268 179 649.0 0 179 269 129 649.0 0 129 270 79 649.0 0 79 271 89 649.0 0 89 272 107 649.0 0 107 273 110 649.0 0 110 274 142 649.0 0 142 275 103 649.0 0 103 276 121 649.0 0 121 277 112 649.0 0 112 278 122 649.0 0 122 279 103 649.0 0 103 280 141 649.0 0 141 281 146 649.0 0 146 282 91 649.0 0 91 283 95 649.0 0 95 284 98 649.0 0 98 285 100 649.0 0 100 286 98 649.0 0 98 287 83 649.0 0 83 288 104 649.0 0 104 289 107 649.0 0 107 290 93 649.0 0 93 291 84 649.0 0 84 292 101 649.0 0 101 293 121 649.0 0 121 294 105 649.0 0 105 295 127 649.0 0 127 296 114 649.0 0 114 297 84 649.0 0 84 298 92 649.0 0 92 299 112 649.0 0 112 300 89 649.0 0 89 301 118 649.0 0 118 Finished in 9.08 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 219,913 Reads with adapters: 13,869 (6.3%) Reads that were too short: 95 (0.0%) Reads written (passing filters): 13,774 (6.3%) Total basepairs processed: 42,386,547 bp Total written (filtered): 3,700,634 bp (8.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 13869 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 3 0.0 3 0 0 1 2 19 55 0.0 3 0 44 5 4 2 20 4207 0.0 4 3893 279 28 4 3 21 8972 0.0 4 8497 366 74 28 7 22 18 0.0 4 4 12 2 23 9 0.0 4 0 0 0 8 1 24 28 0.0 4 0 0 0 25 3 25 3 0.0 4 2 0 0 0 1 31 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 52 2 0.0 4 0 0 0 1 1 57 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 64 2 0.0 4 0 0 0 0 2 69 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 71 3 0.0 4 0 0 0 0 3 73 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 91 7 0.0 4 0 0 0 0 7 92 6 0.0 4 0 0 0 0 6 93 3 0.0 4 0 0 0 0 3 95 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 101 2 0.0 4 0 0 0 0 2 112 18 0.0 4 0 0 0 0 18 114 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 119 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 126 8 0.0 4 0 0 0 0 8 127 393 0.0 4 0 0 0 0 393 128 69 0.0 4 0 0 0 0 69 129 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 136 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 141 4 0.0 4 0 0 0 0 4 142 2 0.0 4 0 0 0 0 2 144 1 0.0 4 0 0 0 0 1 145 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 164 2 0.0 4 0 0 0 0 2 171 8 0.0 4 0 0 0 0 8 173 2 0.0 4 0 0 0 0 2 175 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.51 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 13,774 Reads with adapters: 3,213 (23.3%) Reads written (passing filters): 13,774 (100.0%) Total basepairs processed: 3,700,634 bp Total written (filtered): 3,663,126 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3213 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 8.2% G: 88.5% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 215.2 0 6 4 1 53.8 0 1 7 1 0.8 0 1 9 848 0.1 0 81 767 10 1040 0.0 1 67 973 11 1276 0.0 1 33 1243 12 14 0.0 1 0 14 13 3 0.0 1 0 3 150 1 0.0 1 1 208 5 0.0 1 2 3 223 18 0.0 1 13 5 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.