This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAATGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.79 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,658,179 Reads with adapters: 236,665 (8.9%) Reads that were too short: 25,135 (0.9%) Reads written (passing filters): 211,530 (8.0%) Total basepairs processed: 800,111,879 bp Total written (filtered): 40,998,775 bp (5.1%) === Adapter 1 === Sequence: TAATGA; Type: regular 5'; Length: 6; Trimmed: 236665 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 344 649.0 0 344 7 14810 649.0 0 14810 8 14541 649.0 0 14541 9 28 649.0 0 28 10 22 649.0 0 22 11 38 649.0 0 38 12 32 649.0 0 32 13 29 649.0 0 29 14 25 649.0 0 25 15 32 649.0 0 32 16 32 649.0 0 32 17 29 649.0 0 29 18 33 649.0 0 33 19 18 649.0 0 18 20 32 649.0 0 32 21 25 649.0 0 25 22 27 649.0 0 27 23 29 649.0 0 29 24 20 649.0 0 20 25 29 649.0 0 29 26 32 649.0 0 32 27 39 649.0 0 39 28 40 649.0 0 40 29 126 649.0 0 126 30 29 649.0 0 29 31 40 649.0 0 40 32 26 649.0 0 26 33 26 649.0 0 26 34 37 649.0 0 37 35 37 649.0 0 37 36 44 649.0 0 44 37 60 649.0 0 60 38 114 649.0 0 114 39 44 649.0 0 44 40 36 649.0 0 36 41 32 649.0 0 32 42 32 649.0 0 32 43 60 649.0 0 60 44 28 649.0 0 28 45 33 649.0 0 33 46 44 649.0 0 44 47 35 649.0 0 35 48 44 649.0 0 44 49 37 649.0 0 37 50 41 649.0 0 41 51 29 649.0 0 29 52 33 649.0 0 33 53 39 649.0 0 39 54 36 649.0 0 36 55 43 649.0 0 43 56 57 649.0 0 57 57 50 649.0 0 50 58 60 649.0 0 60 59 76 649.0 0 76 60 55 649.0 0 55 61 52 649.0 0 52 62 53 649.0 0 53 63 57 649.0 0 57 64 183 649.0 0 183 65 47 649.0 0 47 66 84 649.0 0 84 67 78 649.0 0 78 68 102 649.0 0 102 69 153 649.0 0 153 70 2211 649.0 0 2211 71 386 649.0 0 386 72 1052 649.0 0 1052 73 64 649.0 0 64 74 46 649.0 0 46 75 49 649.0 0 49 76 48 649.0 0 48 77 52 649.0 0 52 78 53 649.0 0 53 79 60 649.0 0 60 80 79 649.0 0 79 81 164 649.0 0 164 82 378 649.0 0 378 83 1605 649.0 0 1605 84 151 649.0 0 151 85 103 649.0 0 103 86 76 649.0 0 76 87 104 649.0 0 104 88 69 649.0 0 69 89 71 649.0 0 71 90 100 649.0 0 100 91 88 649.0 0 88 92 103 649.0 0 103 93 74 649.0 0 74 94 140 649.0 0 140 95 104 649.0 0 104 96 84 649.0 0 84 97 172 649.0 0 172 98 205 649.0 0 205 99 137 649.0 0 137 100 229 649.0 0 229 101 168 649.0 0 168 102 106 649.0 0 106 103 109 649.0 0 109 104 110 649.0 0 110 105 95 649.0 0 95 106 130 649.0 0 130 107 277 649.0 0 277 108 258 649.0 0 258 109 276 649.0 0 276 110 441 649.0 0 441 111 915 649.0 0 915 112 360 649.0 0 360 113 354 649.0 0 354 114 734 649.0 0 734 115 349 649.0 0 349 116 341 649.0 0 341 117 630 649.0 0 630 118 613 649.0 0 613 119 714 649.0 0 714 120 777 649.0 0 777 121 1184 649.0 0 1184 122 1299 649.0 0 1299 123 2269 649.0 0 2269 124 61408 649.0 0 61408 125 22001 649.0 0 22001 126 60185 649.0 0 60185 127 1443 649.0 0 1443 128 129 649.0 0 129 129 119 649.0 0 119 130 115 649.0 0 115 131 110 649.0 0 110 132 82 649.0 0 82 133 92 649.0 0 92 134 95 649.0 0 95 135 142 649.0 0 142 136 135 649.0 0 135 137 132 649.0 0 132 138 147 649.0 0 147 139 185 649.0 0 185 140 420 649.0 0 420 141 307 649.0 0 307 142 695 649.0 0 695 143 121 649.0 0 121 144 131 649.0 0 131 145 122 649.0 0 122 146 117 649.0 0 117 147 112 649.0 0 112 148 148 649.0 0 148 149 1696 649.0 0 1696 150 866 649.0 0 866 151 1641 649.0 0 1641 152 98 649.0 0 98 153 79 649.0 0 79 154 92 649.0 0 92 155 103 649.0 0 103 156 131 649.0 0 131 157 114 649.0 0 114 158 139 649.0 0 139 159 123 649.0 0 123 160 69 649.0 0 69 161 104 649.0 0 104 162 97 649.0 0 97 163 178 649.0 0 178 164 173 649.0 0 173 165 128 649.0 0 128 166 104 649.0 0 104 167 92 649.0 0 92 168 85 649.0 0 85 169 89 649.0 0 89 170 111 649.0 0 111 171 111 649.0 0 111 172 78 649.0 0 78 173 72 649.0 0 72 174 73 649.0 0 73 175 63 649.0 0 63 176 76 649.0 0 76 177 88 649.0 0 88 178 111 649.0 0 111 179 93 649.0 0 93 180 95 649.0 0 95 181 190 649.0 0 190 182 161 649.0 0 161 183 136 649.0 0 136 184 98 649.0 0 98 185 81 649.0 0 81 186 106 649.0 0 106 187 94 649.0 0 94 188 193 649.0 0 193 189 101 649.0 0 101 190 179 649.0 0 179 191 88 649.0 0 88 192 80 649.0 0 80 193 73 649.0 0 73 194 107 649.0 0 107 195 89 649.0 0 89 196 87 649.0 0 87 197 93 649.0 0 93 198 111 649.0 0 111 199 81 649.0 0 81 200 93 649.0 0 93 201 80 649.0 0 80 202 87 649.0 0 87 203 101 649.0 0 101 204 130 649.0 0 130 205 131 649.0 0 131 206 118 649.0 0 118 207 137 649.0 0 137 208 151 649.0 0 151 209 110 649.0 0 110 210 143 649.0 0 143 211 111 649.0 0 111 212 125 649.0 0 125 213 106 649.0 0 106 214 120 649.0 0 120 215 88 649.0 0 88 216 109 649.0 0 109 217 168 649.0 0 168 218 159 649.0 0 159 219 162 649.0 0 162 220 127 649.0 0 127 221 199 649.0 0 199 222 197 649.0 0 197 223 970 649.0 0 970 224 1206 649.0 0 1206 225 3942 649.0 0 3942 226 4670 649.0 0 4670 227 3873 649.0 0 3873 228 104 649.0 0 104 229 83 649.0 0 83 230 100 649.0 0 100 231 77 649.0 0 77 232 103 649.0 0 103 233 86 649.0 0 86 234 113 649.0 0 113 235 140 649.0 0 140 236 163 649.0 0 163 237 126 649.0 0 126 238 95 649.0 0 95 239 90 649.0 0 90 240 110 649.0 0 110 241 90 649.0 0 90 242 109 649.0 0 109 243 127 649.0 0 127 244 148 649.0 0 148 245 137 649.0 0 137 246 99 649.0 0 99 247 81 649.0 0 81 248 82 649.0 0 82 249 89 649.0 0 89 250 78 649.0 0 78 251 89 649.0 0 89 252 106 649.0 0 106 253 126 649.0 0 126 254 121 649.0 0 121 255 111 649.0 0 111 256 151 649.0 0 151 257 123 649.0 0 123 258 113 649.0 0 113 259 102 649.0 0 102 260 122 649.0 0 122 261 104 649.0 0 104 262 114 649.0 0 114 263 113 649.0 0 113 264 121 649.0 0 121 265 108 649.0 0 108 266 108 649.0 0 108 267 110 649.0 0 110 268 129 649.0 0 129 269 70 649.0 0 70 270 81 649.0 0 81 271 75 649.0 0 75 272 92 649.0 0 92 273 103 649.0 0 103 274 98 649.0 0 98 275 100 649.0 0 100 276 83 649.0 0 83 277 98 649.0 0 98 278 116 649.0 0 116 279 99 649.0 0 99 280 95 649.0 0 95 281 100 649.0 0 100 282 103 649.0 0 103 283 93 649.0 0 93 284 90 649.0 0 90 285 110 649.0 0 110 286 70 649.0 0 70 287 86 649.0 0 86 288 104 649.0 0 104 289 96 649.0 0 96 290 71 649.0 0 71 291 82 649.0 0 82 292 89 649.0 0 89 293 135 649.0 0 135 294 93 649.0 0 93 295 123 649.0 0 123 296 98 649.0 0 98 297 95 649.0 0 95 298 132 649.0 0 132 299 103 649.0 0 103 300 103 649.0 0 103 301 108 649.0 0 108 Finished in 7.71 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 211,530 Reads with adapters: 15,873 (7.5%) Reads that were too short: 75 (0.0%) Reads written (passing filters): 15,798 (7.5%) Total basepairs processed: 40,998,775 bp Total written (filtered): 4,190,616 bp (10.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15873 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 32 0.0 3 0 28 3 1 17 3936 0.0 3 3786 144 5 1 18 10703 0.0 3 10473 222 7 1 19 25 0.0 3 2 18 1 4 20 11 0.0 3 0 0 4 7 21 11 0.0 3 0 0 0 11 24 1 0.0 3 1 28 1 0.0 3 0 0 0 1 29 4 0.0 3 1 0 0 3 32 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 270 0.0 3 0 0 0 270 44 1 0.0 3 0 0 1 45 53 0.0 3 1 0 2 50 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 11 0.0 3 0 0 1 10 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 12 0.0 3 0 0 0 12 67 1 0.0 3 0 0 0 1 68 703 0.0 3 0 0 1 702 69 4 0.0 3 0 0 0 4 70 3 0.0 3 0 0 0 3 71 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 102 13 0.0 3 0 0 0 13 105 1 0.0 3 0 0 0 1 111 3 0.0 3 0 0 0 3 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 1 1 142 3 0.0 3 0 0 0 3 143 1 0.0 3 0 0 0 1 144 7 0.0 3 0 0 1 6 145 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 157 3 0.0 3 0 0 1 2 164 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 218 13 0.0 3 0 0 0 13 239 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.55 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 15,798 Reads with adapters: 226 (1.4%) Reads written (passing filters): 15,798 (100.0%) Total basepairs processed: 4,190,616 bp Total written (filtered): 4,189,871 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 226 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 58.4% C: 7.1% G: 30.1% T: 4.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 162 246.8 0 162 4 62 61.7 0 62 5 1 15.4 0 1 6 1 3.9 0 1