This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 58.79 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 74,281 (3.3%) Reads that were too short: 10,248 (0.5%) Reads written (passing filters): 64,033 (2.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 14,920,730 bp (2.2%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 74281 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 243 546.6 0 243 7 208 546.6 0 208 8 212 546.6 0 212 9 119 546.6 0 119 10 2177 546.6 0 2177 11 35 546.6 0 35 12 62 546.6 0 62 13 11 546.6 0 11 14 16 546.6 0 16 15 10 546.6 0 10 16 14 546.6 0 14 17 6 546.6 0 6 18 34 546.6 0 34 19 18 546.6 0 18 20 20 546.6 0 20 21 46 546.6 0 46 22 32 546.6 0 32 23 21 546.6 0 21 24 17 546.6 0 17 25 30 546.6 0 30 26 24 546.6 0 24 27 26 546.6 0 26 28 73 546.6 0 73 29 18 546.6 0 18 30 28 546.6 0 28 31 23 546.6 0 23 32 18 546.6 0 18 33 17 546.6 0 17 34 28 546.6 0 28 35 17 546.6 0 17 36 17 546.6 0 17 37 20 546.6 0 20 38 85 546.6 0 85 39 23 546.6 0 23 40 90 546.6 0 90 41 49 546.6 0 49 42 52 546.6 0 52 43 114 546.6 0 114 44 19 546.6 0 19 45 20 546.6 0 20 46 17 546.6 0 17 47 40 546.6 0 40 48 90 546.6 0 90 49 84 546.6 0 84 50 124 546.6 0 124 51 267 546.6 0 267 52 312 546.6 0 312 53 521 546.6 0 521 54 1437 546.6 0 1437 55 14389 546.6 0 14389 56 3828 546.6 0 3828 57 25910 546.6 0 25910 58 105 546.6 0 105 59 21 546.6 0 21 60 24 546.6 0 24 61 26 546.6 0 26 62 31 546.6 0 31 63 27 546.6 0 27 64 14 546.6 0 14 65 33 546.6 0 33 66 18 546.6 0 18 67 14 546.6 0 14 68 20 546.6 0 20 69 12 546.6 0 12 70 30 546.6 0 30 71 34 546.6 0 34 72 16 546.6 0 16 73 20 546.6 0 20 74 33 546.6 0 33 75 25 546.6 0 25 76 150 546.6 0 150 77 26 546.6 0 26 78 39 546.6 0 39 79 30 546.6 0 30 80 38 546.6 0 38 81 34 546.6 0 34 82 43 546.6 0 43 83 66 546.6 0 66 84 93 546.6 0 93 85 1084 546.6 0 1084 86 434 546.6 0 434 87 1338 546.6 0 1338 88 51 546.6 0 51 89 70 546.6 0 70 90 38 546.6 0 38 91 22 546.6 0 22 92 23 546.6 0 23 93 21 546.6 0 21 94 42 546.6 0 42 95 18 546.6 0 18 96 16 546.6 0 16 97 33 546.6 0 33 98 26 546.6 0 26 99 42 546.6 0 42 100 73 546.6 0 73 101 63 546.6 0 63 102 536 546.6 0 536 103 120 546.6 0 120 104 148 546.6 0 148 105 29 546.6 0 29 106 22 546.6 0 22 107 32 546.6 0 32 108 22 546.6 0 22 109 32 546.6 0 32 110 22 546.6 0 22 111 26 546.6 0 26 112 22 546.6 0 22 113 22 546.6 0 22 114 17 546.6 0 17 115 26 546.6 0 26 116 43 546.6 0 43 117 36 546.6 0 36 118 612 546.6 0 612 119 90 546.6 0 90 120 145 546.6 0 145 121 26 546.6 0 26 122 20 546.6 0 20 123 27 546.6 0 27 124 35 546.6 0 35 125 24 546.6 0 24 126 29 546.6 0 29 127 24 546.6 0 24 128 40 546.6 0 40 129 34 546.6 0 34 130 52 546.6 0 52 131 33 546.6 0 33 132 55 546.6 0 55 133 83 546.6 0 83 134 60 546.6 0 60 135 51 546.6 0 51 136 62 546.6 0 62 137 134 546.6 0 134 138 171 546.6 0 171 139 381 546.6 0 381 140 173 546.6 0 173 141 126 546.6 0 126 142 96 546.6 0 96 143 31 546.6 0 31 144 53 546.6 0 53 145 28 546.6 0 28 146 43 546.6 0 43 147 57 546.6 0 57 148 60 546.6 0 60 149 41 546.6 0 41 150 41 546.6 0 41 151 31 546.6 0 31 152 40 546.6 0 40 153 49 546.6 0 49 154 56 546.6 0 56 155 30 546.6 0 30 156 36 546.6 0 36 157 234 546.6 0 234 158 130 546.6 0 130 159 314 546.6 0 314 160 37 546.6 0 37 161 47 546.6 0 47 162 36 546.6 0 36 163 55 546.6 0 55 164 67 546.6 0 67 165 115 546.6 0 115 166 156 546.6 0 156 167 105 546.6 0 105 168 143 546.6 0 143 169 139 546.6 0 139 170 79 546.6 0 79 171 66 546.6 0 66 172 143 546.6 0 143 173 31 546.6 0 31 174 44 546.6 0 44 175 36 546.6 0 36 176 37 546.6 0 37 177 36 546.6 0 36 178 31 546.6 0 31 179 30 546.6 0 30 180 42 546.6 0 42 181 42 546.6 0 42 182 55 546.6 0 55 183 87 546.6 0 87 184 115 546.6 0 115 185 137 546.6 0 137 186 47 546.6 0 47 187 247 546.6 0 247 188 233 546.6 0 233 189 419 546.6 0 419 190 170 546.6 0 170 191 52 546.6 0 52 192 41 546.6 0 41 193 18 546.6 0 18 194 44 546.6 0 44 195 20 546.6 0 20 196 28 546.6 0 28 197 23 546.6 0 23 198 38 546.6 0 38 199 79 546.6 0 79 200 71 546.6 0 71 201 53 546.6 0 53 202 36 546.6 0 36 203 36 546.6 0 36 204 35 546.6 0 35 205 47 546.6 0 47 206 67 546.6 0 67 207 92 546.6 0 92 208 72 546.6 0 72 209 25 546.6 0 25 210 24 546.6 0 24 211 32 546.6 0 32 212 33 546.6 0 33 213 51 546.6 0 51 214 43 546.6 0 43 215 59 546.6 0 59 216 39 546.6 0 39 217 40 546.6 0 40 218 75 546.6 0 75 219 430 546.6 0 430 220 155 546.6 0 155 221 236 546.6 0 236 222 17 546.6 0 17 223 42 546.6 0 42 224 48 546.6 0 48 225 54 546.6 0 54 226 35 546.6 0 35 227 23 546.6 0 23 228 20 546.6 0 20 229 32 546.6 0 32 230 37 546.6 0 37 231 41 546.6 0 41 232 23 546.6 0 23 233 38 546.6 0 38 234 47 546.6 0 47 235 102 546.6 0 102 236 141 546.6 0 141 237 52 546.6 0 52 238 45 546.6 0 45 239 83 546.6 0 83 240 117 546.6 0 117 241 79 546.6 0 79 242 59 546.6 0 59 243 40 546.6 0 40 244 31 546.6 0 31 245 29 546.6 0 29 246 47 546.6 0 47 247 48 546.6 0 48 248 43 546.6 0 43 249 71 546.6 0 71 250 75 546.6 0 75 251 97 546.6 0 97 252 34 546.6 0 34 253 41 546.6 0 41 254 26 546.6 0 26 255 36 546.6 0 36 256 30 546.6 0 30 257 46 546.6 0 46 258 80 546.6 0 80 259 152 546.6 0 152 260 138 546.6 0 138 261 156 546.6 0 156 262 197 546.6 0 197 263 38 546.6 0 38 264 32 546.6 0 32 265 41 546.6 0 41 266 61 546.6 0 61 267 63 546.6 0 63 268 64 546.6 0 64 269 112 546.6 0 112 270 47 546.6 0 47 271 35 546.6 0 35 272 66 546.6 0 66 273 52 546.6 0 52 274 101 546.6 0 101 275 79 546.6 0 79 276 316 546.6 0 316 277 57 546.6 0 57 278 445 546.6 0 445 279 141 546.6 0 141 280 298 546.6 0 298 281 143 546.6 0 143 282 98 546.6 0 98 283 206 546.6 0 206 284 78 546.6 0 78 285 76 546.6 0 76 286 69 546.6 0 69 287 115 546.6 0 115 288 284 546.6 0 284 289 265 546.6 0 265 290 82 546.6 0 82 291 90 546.6 0 90 292 114 546.6 0 114 293 111 546.6 0 111 294 113 546.6 0 113 295 140 546.6 0 140 296 214 546.6 0 214 297 136 546.6 0 136 298 330 546.6 0 330 299 435 546.6 0 435 300 809 546.6 0 809 301 113 546.6 0 113 Finished in 2.91 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 64,033 Reads with adapters: 456 (0.7%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 439 (0.7%) Total basepairs processed: 14,920,730 bp Total written (filtered): 113,847 bp (0.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 456 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 20 157 0.0 4 143 10 2 1 1 21 13 0.0 4 7 2 1 1 2 22 3 0.0 4 0 1 1 0 1 23 211 0.0 4 193 12 4 2 24 3 0.0 4 0 1 0 2 44 1 0.0 4 1 45 1 0.0 4 0 0 0 1 53 1 0.0 4 0 0 0 0 1 54 3 0.0 4 0 0 0 0 3 55 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 68 1 0.0 4 1 78 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 1 116 1 0.0 4 0 0 0 1 119 1 0.0 4 0 0 0 1 123 3 0.0 4 0 0 0 0 3 124 41 0.0 4 0 0 0 0 41 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (68 us/read; 0.88 M reads/minute). === Summary === Total reads processed: 439 Reads with adapters: 87 (19.8%) Reads written (passing filters): 439 (100.0%) Total basepairs processed: 113,847 bp Total written (filtered): 112,811 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 87 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.7% C: 11.5% G: 80.5% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 6.9 0 1 8 1 0.0 0 1 9 7 0.0 0 2 5 10 37 0.0 1 1 36 11 39 0.0 1 0 39 12 1 0.0 1 0 1 151 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.