This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 57.26 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,238,925 Reads with adapters: 72,722 (3.2%) Reads that were too short: 7,089 (0.3%) Reads written (passing filters): 65,633 (2.9%) Total basepairs processed: 673,916,425 bp Total written (filtered): 15,752,426 bp (2.3%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 72722 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 256 546.6 0 256 7 163 546.6 0 163 8 241 546.6 0 241 9 80 546.6 0 80 10 1970 546.6 0 1970 11 21 546.6 0 21 12 43 546.6 0 43 13 10 546.6 0 10 14 17 546.6 0 17 15 10 546.6 0 10 16 9 546.6 0 9 17 10 546.6 0 10 18 34 546.6 0 34 19 19 546.6 0 19 20 18 546.6 0 18 21 26 546.6 0 26 22 13 546.6 0 13 23 13 546.6 0 13 24 13 546.6 0 13 25 17 546.6 0 17 26 21 546.6 0 21 27 28 546.6 0 28 28 67 546.6 0 67 29 16 546.6 0 16 30 13 546.6 0 13 31 16 546.6 0 16 32 13 546.6 0 13 33 11 546.6 0 11 34 20 546.6 0 20 35 29 546.6 0 29 36 12 546.6 0 12 37 31 546.6 0 31 38 108 546.6 0 108 39 33 546.6 0 33 40 87 546.6 0 87 41 40 546.6 0 40 42 54 546.6 0 54 43 156 546.6 0 156 44 26 546.6 0 26 45 22 546.6 0 22 46 23 546.6 0 23 47 26 546.6 0 26 48 89 546.6 0 89 49 58 546.6 0 58 50 121 546.6 0 121 51 293 546.6 0 293 52 324 546.6 0 324 53 530 546.6 0 530 54 1574 546.6 0 1574 55 16548 546.6 0 16548 56 4057 546.6 0 4057 57 30677 546.6 0 30677 58 112 546.6 0 112 59 18 546.6 0 18 60 18 546.6 0 18 61 18 546.6 0 18 62 39 546.6 0 39 63 15 546.6 0 15 64 13 546.6 0 13 65 19 546.6 0 19 66 13 546.6 0 13 67 10 546.6 0 10 68 16 546.6 0 16 69 11 546.6 0 11 70 14 546.6 0 14 71 12 546.6 0 12 72 14 546.6 0 14 73 15 546.6 0 15 74 9 546.6 0 9 75 13 546.6 0 13 76 93 546.6 0 93 77 12 546.6 0 12 78 19 546.6 0 19 79 30 546.6 0 30 80 15 546.6 0 15 81 21 546.6 0 21 82 26 546.6 0 26 83 42 546.6 0 42 84 60 546.6 0 60 85 749 546.6 0 749 86 484 546.6 0 484 87 1364 546.6 0 1364 88 10 546.6 0 10 89 12 546.6 0 12 90 15 546.6 0 15 91 18 546.6 0 18 92 9 546.6 0 9 93 13 546.6 0 13 94 37 546.6 0 37 95 9 546.6 0 9 96 15 546.6 0 15 97 10 546.6 0 10 98 16 546.6 0 16 99 18 546.6 0 18 100 35 546.6 0 35 101 17 546.6 0 17 102 157 546.6 0 157 103 45 546.6 0 45 104 39 546.6 0 39 105 24 546.6 0 24 106 19 546.6 0 19 107 11 546.6 0 11 108 15 546.6 0 15 109 15 546.6 0 15 110 29 546.6 0 29 111 9 546.6 0 9 112 11 546.6 0 11 113 8 546.6 0 8 114 14 546.6 0 14 115 10 546.6 0 10 116 18 546.6 0 18 117 22 546.6 0 22 118 533 546.6 0 533 119 75 546.6 0 75 120 91 546.6 0 91 121 15 546.6 0 15 122 13 546.6 0 13 123 11 546.6 0 11 124 18 546.6 0 18 125 8 546.6 0 8 126 9 546.6 0 9 127 17 546.6 0 17 128 18 546.6 0 18 129 23 546.6 0 23 130 36 546.6 0 36 131 11 546.6 0 11 132 18 546.6 0 18 133 25 546.6 0 25 134 24 546.6 0 24 135 22 546.6 0 22 136 34 546.6 0 34 137 42 546.6 0 42 138 71 546.6 0 71 139 111 546.6 0 111 140 35 546.6 0 35 141 42 546.6 0 42 142 32 546.6 0 32 143 20 546.6 0 20 144 18 546.6 0 18 145 15 546.6 0 15 146 17 546.6 0 17 147 53 546.6 0 53 148 34 546.6 0 34 149 22 546.6 0 22 150 21 546.6 0 21 151 20 546.6 0 20 152 29 546.6 0 29 153 10 546.6 0 10 154 23 546.6 0 23 155 22 546.6 0 22 156 15 546.6 0 15 157 77 546.6 0 77 158 60 546.6 0 60 159 140 546.6 0 140 160 17 546.6 0 17 161 23 546.6 0 23 162 18 546.6 0 18 163 29 546.6 0 29 164 38 546.6 0 38 165 47 546.6 0 47 166 74 546.6 0 74 167 44 546.6 0 44 168 67 546.6 0 67 169 61 546.6 0 61 170 22 546.6 0 22 171 30 546.6 0 30 172 27 546.6 0 27 173 27 546.6 0 27 174 14 546.6 0 14 175 20 546.6 0 20 176 12 546.6 0 12 177 28 546.6 0 28 178 18 546.6 0 18 179 11 546.6 0 11 180 17 546.6 0 17 181 26 546.6 0 26 182 24 546.6 0 24 183 31 546.6 0 31 184 46 546.6 0 46 185 119 546.6 0 119 186 19 546.6 0 19 187 112 546.6 0 112 188 87 546.6 0 87 189 187 546.6 0 187 190 73 546.6 0 73 191 43 546.6 0 43 192 28 546.6 0 28 193 12 546.6 0 12 194 16 546.6 0 16 195 7 546.6 0 7 196 7 546.6 0 7 197 17 546.6 0 17 198 15 546.6 0 15 199 54 546.6 0 54 200 25 546.6 0 25 201 31 546.6 0 31 202 9 546.6 0 9 203 13 546.6 0 13 204 14 546.6 0 14 205 18 546.6 0 18 206 24 546.6 0 24 207 21 546.6 0 21 208 69 546.6 0 69 209 20 546.6 0 20 210 13 546.6 0 13 211 13 546.6 0 13 212 15 546.6 0 15 213 21 546.6 0 21 214 15 546.6 0 15 215 14 546.6 0 14 216 9 546.6 0 9 217 17 546.6 0 17 218 22 546.6 0 22 219 258 546.6 0 258 220 82 546.6 0 82 221 116 546.6 0 116 222 6 546.6 0 6 223 5 546.6 0 5 224 22 546.6 0 22 225 21 546.6 0 21 226 17 546.6 0 17 227 14 546.6 0 14 228 8 546.6 0 8 229 21 546.6 0 21 230 15 546.6 0 15 231 23 546.6 0 23 232 9 546.6 0 9 233 17 546.6 0 17 234 21 546.6 0 21 235 36 546.6 0 36 236 43 546.6 0 43 237 25 546.6 0 25 238 23 546.6 0 23 239 26 546.6 0 26 240 42 546.6 0 42 241 38 546.6 0 38 242 12 546.6 0 12 243 22 546.6 0 22 244 15 546.6 0 15 245 15 546.6 0 15 246 13 546.6 0 13 247 27 546.6 0 27 248 15 546.6 0 15 249 25 546.6 0 25 250 35 546.6 0 35 251 35 546.6 0 35 252 9 546.6 0 9 253 11 546.6 0 11 254 11 546.6 0 11 255 14 546.6 0 14 256 14 546.6 0 14 257 17 546.6 0 17 258 34 546.6 0 34 259 69 546.6 0 69 260 55 546.6 0 55 261 60 546.6 0 60 262 77 546.6 0 77 263 18 546.6 0 18 264 11 546.6 0 11 265 17 546.6 0 17 266 23 546.6 0 23 267 28 546.6 0 28 268 24 546.6 0 24 269 22 546.6 0 22 270 26 546.6 0 26 271 22 546.6 0 22 272 38 546.6 0 38 273 27 546.6 0 27 274 47 546.6 0 47 275 66 546.6 0 66 276 613 546.6 0 613 277 67 546.6 0 67 278 1009 546.6 0 1009 279 55 546.6 0 55 280 243 546.6 0 243 281 70 546.6 0 70 282 47 546.6 0 47 283 48 546.6 0 48 284 40 546.6 0 40 285 36 546.6 0 36 286 35 546.6 0 35 287 54 546.6 0 54 288 118 546.6 0 118 289 107 546.6 0 107 290 50 546.6 0 50 291 55 546.6 0 55 292 46 546.6 0 46 293 59 546.6 0 59 294 46 546.6 0 46 295 59 546.6 0 59 296 93 546.6 0 93 297 81 546.6 0 81 298 370 546.6 0 370 299 546 546.6 0 546 300 924 546.6 0 924 301 49 546.6 0 49 Finished in 2.70 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 65,633 Reads with adapters: 182 (0.3%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 169 (0.3%) Total basepairs processed: 15,752,426 bp Total written (filtered): 41,606 bp (0.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 182 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 136 0.0 3 133 3 30 2 0.0 3 0 0 0 2 34 6 0.0 3 0 0 0 6 37 1 0.0 3 0 0 0 1 43 1 0.0 3 1 44 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 106 1 0.0 3 1 112 1 0.0 3 0 1 116 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 169 1 0.0 3 0 1 171 1 0.0 3 1 181 4 0.0 3 0 0 0 4 182 11 0.0 3 0 0 0 11 184 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 169 Reads with adapters: 0 (0.0%) Reads written (passing filters): 169 (100.0%) Total basepairs processed: 41,606 bp Total written (filtered): 41,606 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 0 times.