This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CATTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.76 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,834,615 Reads with adapters: 102,874 (3.6%) Reads that were too short: 12,205 (0.4%) Reads written (passing filters): 90,669 (3.2%) Total basepairs processed: 853,219,115 bp Total written (filtered): 18,529,793 bp (2.2%) === Adapter 1 === Sequence: CATTAG; Type: regular 5'; Length: 6; Trimmed: 102874 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 581 692.0 0 581 7 13770 692.0 0 13770 8 11133 692.0 0 11133 9 92 692.0 0 92 10 73 692.0 0 73 11 44 692.0 0 44 12 34 692.0 0 34 13 39 692.0 0 39 14 63 692.0 0 63 15 60 692.0 0 60 16 55 692.0 0 55 17 50 692.0 0 50 18 61 692.0 0 61 19 43 692.0 0 43 20 54 692.0 0 54 21 53 692.0 0 53 22 46 692.0 0 46 23 57 692.0 0 57 24 42 692.0 0 42 25 44 692.0 0 44 26 66 692.0 0 66 27 231 692.0 0 231 28 328 692.0 0 328 29 463 692.0 0 463 30 42 692.0 0 42 31 36 692.0 0 36 32 43 692.0 0 43 33 39 692.0 0 39 34 43 692.0 0 43 35 66 692.0 0 66 36 67 692.0 0 67 37 50 692.0 0 50 38 52 692.0 0 52 39 56 692.0 0 56 40 32 692.0 0 32 41 57 692.0 0 57 42 46 692.0 0 46 43 46 692.0 0 46 44 53 692.0 0 53 45 55 692.0 0 55 46 47 692.0 0 47 47 42 692.0 0 42 48 47 692.0 0 47 49 45 692.0 0 45 50 70 692.0 0 70 51 53 692.0 0 53 52 67 692.0 0 67 53 47 692.0 0 47 54 58 692.0 0 58 55 60 692.0 0 60 56 104 692.0 0 104 57 99 692.0 0 99 58 673 692.0 0 673 59 257 692.0 0 257 60 352 692.0 0 352 61 37 692.0 0 37 62 52 692.0 0 52 63 35 692.0 0 35 64 45 692.0 0 45 65 44 692.0 0 44 66 44 692.0 0 44 67 49 692.0 0 49 68 59 692.0 0 59 69 80 692.0 0 80 70 146 692.0 0 146 71 144 692.0 0 144 72 1350 692.0 0 1350 73 366 692.0 0 366 74 863 692.0 0 863 75 59 692.0 0 59 76 57 692.0 0 57 77 61 692.0 0 61 78 60 692.0 0 60 79 62 692.0 0 62 80 64 692.0 0 64 81 80 692.0 0 80 82 39 692.0 0 39 83 46 692.0 0 46 84 61 692.0 0 61 85 49 692.0 0 49 86 44 692.0 0 44 87 48 692.0 0 48 88 101 692.0 0 101 89 57 692.0 0 57 90 146 692.0 0 146 91 108 692.0 0 108 92 289 692.0 0 289 93 56 692.0 0 56 94 47 692.0 0 47 95 81 692.0 0 81 96 63 692.0 0 63 97 41 692.0 0 41 98 106 692.0 0 106 99 67 692.0 0 67 100 41 692.0 0 41 101 67 692.0 0 67 102 42 692.0 0 42 103 46 692.0 0 46 104 50 692.0 0 50 105 40 692.0 0 40 106 42 692.0 0 42 107 53 692.0 0 53 108 88 692.0 0 88 109 100 692.0 0 100 110 110 692.0 0 110 111 61 692.0 0 61 112 385 692.0 0 385 113 222 692.0 0 222 114 1115 692.0 0 1115 115 41 692.0 0 41 116 57 692.0 0 57 117 78 692.0 0 78 118 51 692.0 0 51 119 102 692.0 0 102 120 81 692.0 0 81 121 86 692.0 0 86 122 57 692.0 0 57 123 63 692.0 0 63 124 67 692.0 0 67 125 77 692.0 0 77 126 105 692.0 0 105 127 99 692.0 0 99 128 157 692.0 0 157 129 280 692.0 0 280 130 504 692.0 0 504 131 233 692.0 0 233 132 148 692.0 0 148 133 325 692.0 0 325 134 477 692.0 0 477 135 158 692.0 0 158 136 212 692.0 0 212 137 267 692.0 0 267 138 205 692.0 0 205 139 219 692.0 0 219 140 325 692.0 0 325 141 528 692.0 0 528 142 607 692.0 0 607 143 6207 692.0 0 6207 144 5592 692.0 0 5592 145 28557 692.0 0 28557 146 213 692.0 0 213 147 93 692.0 0 93 148 92 692.0 0 92 149 110 692.0 0 110 150 182 692.0 0 182 151 43 692.0 0 43 152 53 692.0 0 53 153 56 692.0 0 56 154 61 692.0 0 61 155 182 692.0 0 182 156 420 692.0 0 420 157 710 692.0 0 710 158 61 692.0 0 61 159 70 692.0 0 70 160 64 692.0 0 64 161 73 692.0 0 73 162 81 692.0 0 81 163 460 692.0 0 460 164 1013 692.0 0 1013 165 612 692.0 0 612 166 63 692.0 0 63 167 55 692.0 0 55 168 68 692.0 0 68 169 82 692.0 0 82 170 59 692.0 0 59 171 45 692.0 0 45 172 39 692.0 0 39 173 52 692.0 0 52 174 43 692.0 0 43 175 66 692.0 0 66 176 50 692.0 0 50 177 58 692.0 0 58 178 49 692.0 0 49 179 53 692.0 0 53 180 54 692.0 0 54 181 45 692.0 0 45 182 57 692.0 0 57 183 56 692.0 0 56 184 63 692.0 0 63 185 69 692.0 0 69 186 70 692.0 0 70 187 52 692.0 0 52 188 68 692.0 0 68 189 44 692.0 0 44 190 66 692.0 0 66 191 58 692.0 0 58 192 54 692.0 0 54 193 73 692.0 0 73 194 48 692.0 0 48 195 58 692.0 0 58 196 89 692.0 0 89 197 60 692.0 0 60 198 56 692.0 0 56 199 37 692.0 0 37 200 51 692.0 0 51 201 91 692.0 0 91 202 70 692.0 0 70 203 116 692.0 0 116 204 85 692.0 0 85 205 68 692.0 0 68 206 79 692.0 0 79 207 74 692.0 0 74 208 116 692.0 0 116 209 165 692.0 0 165 210 164 692.0 0 164 211 192 692.0 0 192 212 102 692.0 0 102 213 263 692.0 0 263 214 350 692.0 0 350 215 766 692.0 0 766 216 372 692.0 0 372 217 408 692.0 0 408 218 68 692.0 0 68 219 49 692.0 0 49 220 56 692.0 0 56 221 111 692.0 0 111 222 84 692.0 0 84 223 131 692.0 0 131 224 68 692.0 0 68 225 77 692.0 0 77 226 102 692.0 0 102 227 74 692.0 0 74 228 69 692.0 0 69 229 69 692.0 0 69 230 73 692.0 0 73 231 276 692.0 0 276 232 246 692.0 0 246 233 660 692.0 0 660 234 149 692.0 0 149 235 108 692.0 0 108 236 166 692.0 0 166 237 71 692.0 0 71 238 43 692.0 0 43 239 57 692.0 0 57 240 66 692.0 0 66 241 48 692.0 0 48 242 127 692.0 0 127 243 73 692.0 0 73 244 111 692.0 0 111 245 109 692.0 0 109 246 67 692.0 0 67 247 54 692.0 0 54 248 55 692.0 0 55 249 68 692.0 0 68 250 46 692.0 0 46 251 50 692.0 0 50 252 58 692.0 0 58 253 85 692.0 0 85 254 78 692.0 0 78 255 83 692.0 0 83 256 53 692.0 0 53 257 49 692.0 0 49 258 72 692.0 0 72 259 67 692.0 0 67 260 84 692.0 0 84 261 131 692.0 0 131 262 66 692.0 0 66 263 113 692.0 0 113 264 150 692.0 0 150 265 208 692.0 0 208 266 89 692.0 0 89 267 63 692.0 0 63 268 53 692.0 0 53 269 89 692.0 0 89 270 104 692.0 0 104 271 114 692.0 0 114 272 74 692.0 0 74 273 68 692.0 0 68 274 67 692.0 0 67 275 82 692.0 0 82 276 59 692.0 0 59 277 80 692.0 0 80 278 88 692.0 0 88 279 84 692.0 0 84 280 92 692.0 0 92 281 90 692.0 0 90 282 178 692.0 0 178 283 103 692.0 0 103 284 132 692.0 0 132 285 106 692.0 0 106 286 139 692.0 0 139 287 76 692.0 0 76 288 62 692.0 0 62 289 73 692.0 0 73 290 81 692.0 0 81 291 104 692.0 0 104 292 104 692.0 0 104 293 110 692.0 0 110 294 104 692.0 0 104 295 175 692.0 0 175 296 334 692.0 0 334 297 284 692.0 0 284 298 141 692.0 0 141 299 83 692.0 0 83 300 86 692.0 0 86 301 66 692.0 0 66 Finished in 4.01 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 90,669 Reads with adapters: 11,919 (13.1%) Reads that were too short: 48 (0.1%) Reads written (passing filters): 11,871 (13.1%) Total basepairs processed: 18,529,793 bp Total written (filtered): 3,240,537 bp (17.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11919 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 7 0.0 3 0 0 5 0 2 19 153 0.0 3 0 113 25 11 4 20 11624 0.0 4 10890 591 105 29 9 21 36 0.0 4 8 20 5 2 1 22 3 0.0 4 0 0 3 23 36 0.0 4 0 0 1 20 15 34 1 0.0 4 0 0 0 1 36 1 0.0 4 0 1 37 1 0.0 4 0 0 1 38 1 0.0 4 1 40 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 1 88 1 0.0 4 0 0 0 0 1 89 3 0.0 4 0 0 0 0 3 92 2 0.0 4 0 0 0 0 2 97 2 0.0 4 0 0 0 0 2 98 5 0.0 4 0 0 0 0 5 104 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 127 2 0.0 4 0 0 0 0 2 128 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 1 140 1 0.0 4 0 0 0 0 1 146 9 0.0 4 0 0 0 0 9 147 4 0.0 4 0 0 0 0 4 148 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 285 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.43 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 11,871 Reads with adapters: 2,336 (19.7%) Reads written (passing filters): 11,871 (100.0%) Total basepairs processed: 3,240,537 bp Total written (filtered): 3,212,947 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2336 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.8% C: 9.2% G: 84.0% T: 3.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 22 185.5 0 22 4 7 46.4 0 7 8 2 0.2 0 2 9 12 0.0 0 0 12 10 134 0.0 1 8 126 11 2119 0.0 1 88 2031 12 26 0.0 1 1 25 14 4 0.0 1 0 4 209 4 0.0 1 4 211 1 0.0 1 1 228 1 0.0 1 1 270 4 0.0 1 3 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.